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The map label for this gene is epsD [H]

Identifier: 85375482

GI number: 85375482

Start: 2672700

End: 2673947

Strand: Direct

Name: epsD [H]

Synonym: ELI_13275

Alternate gene names: 85375482

Gene position: 2672700-2673947 (Clockwise)

Preceding gene: 85375480

Following gene: 85375483

Centisome position: 87.56

GC content: 60.82

Gene sequence:

>1248_bases
ATGGCGCGGGCGGGATGCTCCGTCACCGGCGTAGATATCTCCGAAGAGGTCGTCGTCACCATAAATCGCGGCGCTATCCA
CATCGAGGAAGTCGACCTCGACGGCCTGGTGAAGGGCGTTGTCAGCCGCGGCGCCTTGCGTGCGTCCACACACATCGTGC
CCGCCGACGTTTTCGTCATCGCAGTGCCGACGCCGTTCGCCAAAGACGGCAAGCATACGCCGGATACGTCTCACGTCATG
GCCGCTGCGACCGATATCGCTGCGGTTCTCAAGAAAGGCGACAGCATTATCCTGGAGTCGACCTGTCCTGTCGGCACGAC
CCATGCCATGCGCGACTTGCTTGCTGGGCTGCGCCCGGACCTCTCCATGCCCGGTCTCTCCGACGGCATTGCCGATATCG
CAATCTCCTATTGCCCGGAACGGGTACTCCCTGGCCGGATCATCGAAGAACTGGTTCATAACGATCGATCCATCGGCGGC
ATCACGCCGCGCTGTGCGCGCAAGGCGATCTCTCTCTACAAGCACTTCGTGAAGGGCGATTGTATCGCGACCGATGCCAA
ATCGGCCGAGATGACCAAGCTGGTCGAGAATGCCTATCGCGACGTCAACATCGCATTCGCAAACGAGCTGTCGATGATTG
CCGCGGCGATGAAGCTCGATGTGTGGGAAGTAATCAGGCTGGCGAACCGTCATCCGAGGGTCGATATCCTTGAGCCGGGC
CCCGGCGTAGGGGGACACTGCATTGCAGTAGATCCCTGGTTTATCGTGCATGGCGCGCCCGAAGAGGCGGTCCTGATCCG
CACAGGGCGCGAGGTGAACGACCGCAAGATGCATCACGTCACAGAGCGAGCCGCCCAGCTGATCGAGGCGCATCCCGACA
AACGTGTCGCGTGCCTCGGGCTTTCGTTCAAAGCCAATGTCGACGATTTTCGCGAAAGCCCGGCTCGTTACGTAGCGGCC
CGGCTCGCCGAGCGCTTCGGTGACAGGCTGAGTATCGTAGAGCCCTATGCGAACATGCTGCCCGCCGAGTTCGACGGTAC
CGGGGCCACGCTTGTCGACCTCGACAGTGCCATTGAGCAAAGCGAGATCATGATCGTCCTGGTCAATCACGACTTGTTCA
AGGCAGTGCCGTTCGACGAACGCGTCGACAAGATCGTTTACGATTTGCGGGGCATTTGGCCCGGGCACGGCGCGCCCGAT
CAGGAAGCGATCGGGGTTCCGGCAAAACTGCTACGAACAGGTACCTGA

Upstream 100 bases:

>100_bases
GACCGAACCTAACGAGGGATGGTTTTGAATGCGTAACGAGTCCGCACTTGAGGTATGTGTTGTCGGGTTAGGCTATATCG
GCCTTCCGACTGCCGCGGTG

Downstream 100 bases:

>100_bases
GTCCCATCTCGAGAAATCGCTAAAGTCGGTCACCCAAGTGTGGCGGCGCTTTTACTTTCGCAATCGATCATCGGCGCAGC
GAGGACGAGGTTTGCGTGGT

Product: UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase

Products: UDP-N-acetyl-D-mannosaminuronic-acid; NADH; Proton [C]

Alternate protein names: NA

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MARAGCSVTGVDISEEVVVTINRGAIHIEEVDLDGLVKGVVSRGALRASTHIVPADVFVIAVPTPFAKDGKHTPDTSHVM
AAATDIAAVLKKGDSIILESTCPVGTTHAMRDLLAGLRPDLSMPGLSDGIADIAISYCPERVLPGRIIEELVHNDRSIGG
ITPRCARKAISLYKHFVKGDCIATDAKSAEMTKLVENAYRDVNIAFANELSMIAAAMKLDVWEVIRLANRHPRVDILEPG
PGVGGHCIAVDPWFIVHGAPEEAVLIRTGREVNDRKMHHVTERAAQLIEAHPDKRVACLGLSFKANVDDFRESPARYVAA
RLAERFGDRLSIVEPYANMLPAEFDGTGATLVDLDSAIEQSEIMIVLVNHDLFKAVPFDERVDKIVYDLRGIWPGHGAPD
QEAIGVPAKLLRTGT

Sequences:

>Translated_415_residues
MARAGCSVTGVDISEEVVVTINRGAIHIEEVDLDGLVKGVVSRGALRASTHIVPADVFVIAVPTPFAKDGKHTPDTSHVM
AAATDIAAVLKKGDSIILESTCPVGTTHAMRDLLAGLRPDLSMPGLSDGIADIAISYCPERVLPGRIIEELVHNDRSIGG
ITPRCARKAISLYKHFVKGDCIATDAKSAEMTKLVENAYRDVNIAFANELSMIAAAMKLDVWEVIRLANRHPRVDILEPG
PGVGGHCIAVDPWFIVHGAPEEAVLIRTGREVNDRKMHHVTERAAQLIEAHPDKRVACLGLSFKANVDDFRESPARYVAA
RLAERFGDRLSIVEPYANMLPAEFDGTGATLVDLDSAIEQSEIMIVLVNHDLFKAVPFDERVDKIVYDLRGIWPGHGAPD
QEAIGVPAKLLRTGT
>Mature_414_residues
ARAGCSVTGVDISEEVVVTINRGAIHIEEVDLDGLVKGVVSRGALRASTHIVPADVFVIAVPTPFAKDGKHTPDTSHVMA
AATDIAAVLKKGDSIILESTCPVGTTHAMRDLLAGLRPDLSMPGLSDGIADIAISYCPERVLPGRIIEELVHNDRSIGGI
TPRCARKAISLYKHFVKGDCIATDAKSAEMTKLVENAYRDVNIAFANELSMIAAAMKLDVWEVIRLANRHPRVDILEPGP
GVGGHCIAVDPWFIVHGAPEEAVLIRTGREVNDRKMHHVTERAAQLIEAHPDKRVACLGLSFKANVDDFRESPARYVAAR
LAERFGDRLSIVEPYANMLPAEFDGTGATLVDLDSAIEQSEIMIVLVNHDLFKAVPFDERVDKIVYDLRGIWPGHGAPDQ
EAIGVPAKLLRTGT

Specific function: Probably involved in the synthesis of sugar components of EPS I, by converting NDP-N-acetyl-D-galactosamine into NDP-N- acetyl-D-galactosaminuronic acid [H]

COG id: COG0677

COG function: function code M; UDP-N-acetyl-D-mannosaminuronate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UDP-glucose/GDP-mannose dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4507813, Length=349, Percent_Identity=26.647564469914, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI296040438, Length=276, Percent_Identity=27.1739130434783, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI296040443, Length=217, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI48994968, Length=395, Percent_Identity=52.1518987341772, Blast_Score=403, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17560350, Length=352, Percent_Identity=25.5681818181818, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17136908, Length=349, Percent_Identity=28.3667621776504, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR016040
- InterPro:   IPR017476
- InterPro:   IPR014027
- InterPro:   IPR014026
- InterPro:   IPR014028
- InterPro:   IPR001732 [H]

Pfam domain/function: PF00984 UDPG_MGDP_dh; PF03720 UDPG_MGDP_dh_C; PF03721 UDPG_MGDP_dh_N [H]

EC number: 1.1.1.- [C]

Molecular weight: Translated: 44864; Mature: 44733

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARAGCSVTGVDISEEVVVTINRGAIHIEEVDLDGLVKGVVSRGALRASTHIVPADVFVI
CCCCCCEEECCCCCCEEEEEEECCEEEEEECCHHHHHHHHHHCCCHHHCCEEEECCEEEE
AVPTPFAKDGKHTPDTSHVMAAATDIAAVLKKGDSIILESTCPVGTTHAMRDLLAGLRPD
EECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHCCCC
LSMPGLSDGIADIAISYCPERVLPGRIIEELVHNDRSIGGITPRCARKAISLYKHFVKGD
CCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCC
CIATDAKSAEMTKLVENAYRDVNIAFANELSMIAAAMKLDVWEVIRLANRHPRVDILEPG
EEECCCCHHHHHHHHHHHHHCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCC
PGVGGHCIAVDPWFIVHGAPEEAVLIRTGREVNDRKMHHVTERAAQLIEAHPDKRVACLG
CCCCCEEEEECCEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEE
LSFKANVDDFRESPARYVAARLAERFGDRLSIVEPYANMLPAEFDGTGATLVDLDSAIEQ
EEEECCCHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHCCCCCCCCCCEEEECHHHHCC
SEIMIVLVNHDLFKAVPFDERVDKIVYDLRGIWPGHGAPDQEAIGVPAKLLRTGT
CCEEEEEECCCHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCC
>Mature Secondary Structure 
ARAGCSVTGVDISEEVVVTINRGAIHIEEVDLDGLVKGVVSRGALRASTHIVPADVFVI
CCCCCEEECCCCCCEEEEEEECCEEEEEECCHHHHHHHHHHCCCHHHCCEEEECCEEEE
AVPTPFAKDGKHTPDTSHVMAAATDIAAVLKKGDSIILESTCPVGTTHAMRDLLAGLRPD
EECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHCCCC
LSMPGLSDGIADIAISYCPERVLPGRIIEELVHNDRSIGGITPRCARKAISLYKHFVKGD
CCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCC
CIATDAKSAEMTKLVENAYRDVNIAFANELSMIAAAMKLDVWEVIRLANRHPRVDILEPG
EEECCCCHHHHHHHHHHHHHCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCC
PGVGGHCIAVDPWFIVHGAPEEAVLIRTGREVNDRKMHHVTERAAQLIEAHPDKRVACLG
CCCCCEEEEECCEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEE
LSFKANVDDFRESPARYVAARLAERFGDRLSIVEPYANMLPAEFDGTGATLVDLDSAIEQ
EEEECCCHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHCCCCCCCCCCEEEECHHHHCC
SEIMIVLVNHDLFKAVPFDERVDKIVYDLRGIWPGHGAPDQEAIGVPAKLLRTGT
CCEEEEEECCCHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-N-acetyl-D-mannosamine; NAD [C]

Specific reaction: UDP-N-acetyl-D-mannosamine + NAD = UDP-N-acetyl-D-mannosaminuronic-acid + NADH + Proton [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]