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The map label for this gene is yisS [H]

Identifier: 85375483

GI number: 85375483

Start: 2674293

End: 2675267

Strand: Direct

Name: yisS [H]

Synonym: ELI_13280

Alternate gene names: 85375483

Gene position: 2674293-2675267 (Clockwise)

Preceding gene: 85375482

Following gene: 85375484

Centisome position: 87.61

GC content: 61.54

Gene sequence:

>975_bases
GTGAGCGAAGCACCGACACCTCGGGTTGCACTGATCGGATGCGGACGTTGGGGCCGCAACATTGCGCGCGTCCTGGCCGG
GCTGGGCGCTCTCGAACTGATTGTCGATCCCATGGCGGACGCGTTATCGGATTATGCTTCGGAGCTTGGCGTGCGCGTAA
CTTCCGACATGACGCAGGCGTTCGCGGACGAAATCGACGCCGTCGCCATCGCCGCTCCGGCTGTCGACCACGGCAGGCTC
GCCAAACTGGCGCTGGAAGCCGGCAAGCCGGTTTATGTAGAAAAACCGCTTGCTCTCGACCTTTCGGAAGCTTCGGAGGT
CGCAGCGCTTGCTGCCGAGAAGGGATTGCCGTTGATGATCGGCCATCTCCTTCAATACCATCCGGCGTTCGTGACACTGA
AGCAAATGGTCGATCAGGGCGCAATCGGCGAGGTGCGTCACATCACCTCGCACCGATTGAACCCAGGTGCCATCCGAACC
GAGGAAAACGCGTTATGGAGCATGGGGCCGCACGATTTTTCCATGACGCTGGGTCTGGCCGGCGAAGAACCGATTTCGGT
CGCAACCCTGCCGGTCCGGGTCGTCAACCCCAACATCCCTGATCAGTACTTCGTCCAGTTACGTTTCCCGAGCGGCATTA
CGGCGCAGGTCGTCGCATCCTGGCTGTCGCCCTACAAAGAACACAAGTTGACCGTTCTCGGGACAGAGGGCGCGCTGGTC
TTCGAGGACACGGCCGCCGAACGCGATCGCAAGCTCGTGATCTATCGTGACTACGTTGATACCAGCGAAGCCACGCCACG
GTTCGCGAAGGGGCCCGGCGAACCGGTGAATTGGCCGGCGGAAGAGCCGCTGGAAAACGAAATGCTTGCTTTTCTGGACG
CAGTTCGCGGCGGCGACGGCTGCCGCACCGGACCGGAAGAAGCCATTCCGGTACTGCGTCTTCTGCATGCGGCGGAAGCA
GCCGCAGAGGCATGA

Upstream 100 bases:

>100_bases
CGGGCGAGGCAATACGTCATCATCTTGCAGTATCAGTACGAATATCGGTCCGGCGTCCTAATCGATTGCGCTTGGCCGCA
ACCGGAACAGGAAGCATGAC

Downstream 100 bases:

>100_bases
CTTGGCGTCTTTCCGACTTTGCTGGCATCGCCGGCCTGCGCGTCGAGCGTGACGGGTCGTTCGAAACTACCGGCAAGCTG
TCGACGCCTCTCGATGGCCT

Product: oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MSEAPTPRVALIGCGRWGRNIARVLAGLGALELIVDPMADALSDYASELGVRVTSDMTQAFADEIDAVAIAAPAVDHGRL
AKLALEAGKPVYVEKPLALDLSEASEVAALAAEKGLPLMIGHLLQYHPAFVTLKQMVDQGAIGEVRHITSHRLNPGAIRT
EENALWSMGPHDFSMTLGLAGEEPISVATLPVRVVNPNIPDQYFVQLRFPSGITAQVVASWLSPYKEHKLTVLGTEGALV
FEDTAAERDRKLVIYRDYVDTSEATPRFAKGPGEPVNWPAEEPLENEMLAFLDAVRGGDGCRTGPEEAIPVLRLLHAAEA
AAEA

Sequences:

>Translated_324_residues
MSEAPTPRVALIGCGRWGRNIARVLAGLGALELIVDPMADALSDYASELGVRVTSDMTQAFADEIDAVAIAAPAVDHGRL
AKLALEAGKPVYVEKPLALDLSEASEVAALAAEKGLPLMIGHLLQYHPAFVTLKQMVDQGAIGEVRHITSHRLNPGAIRT
EENALWSMGPHDFSMTLGLAGEEPISVATLPVRVVNPNIPDQYFVQLRFPSGITAQVVASWLSPYKEHKLTVLGTEGALV
FEDTAAERDRKLVIYRDYVDTSEATPRFAKGPGEPVNWPAEEPLENEMLAFLDAVRGGDGCRTGPEEAIPVLRLLHAAEA
AAEA
>Mature_323_residues
SEAPTPRVALIGCGRWGRNIARVLAGLGALELIVDPMADALSDYASELGVRVTSDMTQAFADEIDAVAIAAPAVDHGRLA
KLALEAGKPVYVEKPLALDLSEASEVAALAAEKGLPLMIGHLLQYHPAFVTLKQMVDQGAIGEVRHITSHRLNPGAIRTE
ENALWSMGPHDFSMTLGLAGEEPISVATLPVRVVNPNIPDQYFVQLRFPSGITAQVVASWLSPYKEHKLTVLGTEGALVF
EDTAAERDRKLVIYRDYVDTSEATPRFAKGPGEPVNWPAEEPLENEMLAFLDAVRGGDGCRTGPEEAIPVLRLLHAAEAA
AEA

Specific function: Unknown

COG id: COG0673

COG function: function code R; Predicted dehydrogenases and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfo/idh/mocA family [H]

Homologues:

Organism=Escherichia coli, GI87081947, Length=152, Percent_Identity=38.8157894736842, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1789848, Length=199, Percent_Identity=30.1507537688442, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI87082405, Length=251, Percent_Identity=23.5059760956175, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000683
- InterPro:   IPR004104 [H]

Pfam domain/function: PF01408 GFO_IDH_MocA; PF02894 GFO_IDH_MocA_C [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 34739; Mature: 34608

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEAPTPRVALIGCGRWGRNIARVLAGLGALELIVDPMADALSDYASELGVRVTSDMTQA
CCCCCCCCEEEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHH
FADEIDAVAIAAPAVDHGRLAKLALEAGKPVYVEKPLALDLSEASEVAALAAEKGLPLMI
HHHHHCEEEEECCCCCCCHHHHHHHHCCCEEEEECCCEECHHHHHHHHHHHHHCCCHHHH
GHLLQYHPAFVTLKQMVDQGAIGEVRHITSHRLNPGAIRTEENALWSMGPHDFSMTLGLA
HHHHHHCHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCEEECCCCEECCCCCCEEEEEECC
GEEPISVATLPVRVVNPNIPDQYFVQLRFPSGITAQVVASWLSPYKEHKLTVLGTEGALV
CCCCCEEEEEEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCEEEEEECCCCEE
FEDTAAERDRKLVIYRDYVDTSEATPRFAKGPGEPVNWPAEEPLENEMLAFLDAVRGGDG
EECCCCCCCCEEEEEECCCCCCCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
CRTGPEEAIPVLRLLHAAEAAAEA
CCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SEAPTPRVALIGCGRWGRNIARVLAGLGALELIVDPMADALSDYASELGVRVTSDMTQA
CCCCCCCEEEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHH
FADEIDAVAIAAPAVDHGRLAKLALEAGKPVYVEKPLALDLSEASEVAALAAEKGLPLMI
HHHHHCEEEEECCCCCCCHHHHHHHHCCCEEEEECCCEECHHHHHHHHHHHHHCCCHHHH
GHLLQYHPAFVTLKQMVDQGAIGEVRHITSHRLNPGAIRTEENALWSMGPHDFSMTLGLA
HHHHHHCHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCEEECCCCEECCCCCCEEEEEECC
GEEPISVATLPVRVVNPNIPDQYFVQLRFPSGITAQVVASWLSPYKEHKLTVLGTEGALV
CCCCCEEEEEEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCEEEEEECCCCEE
FEDTAAERDRKLVIYRDYVDTSEATPRFAKGPGEPVNWPAEEPLENEMLAFLDAVRGGDG
EECCCCCCCCEEEEEECCCCCCCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
CRTGPEEAIPVLRLLHAAEAAAEA
CCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353932; 9384377; 8407808 [H]