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The map label for this gene is mnmG

Identifier: 85375438

GI number: 85375438

Start: 2627917

End: 2629782

Strand: Direct

Name: mnmG

Synonym: ELI_13055

Alternate gene names: 85375438

Gene position: 2627917-2629782 (Clockwise)

Preceding gene: 85375437

Following gene: 85375439

Centisome position: 86.09

GC content: 66.99

Gene sequence:

>1866_bases
ATGGGCCGCGCCATGCACGATTTCGACATCCTTGTGGTCGGCGGCGGACACGCCGGCGTCGAGGCGGCCTGCGCGGCGGC
ACGGATGGGCGTGCGCACCGCGCTGGTGAGCTTCGACCCGGCCAGGATTGGCGCGATGAGTTGCAATCCGGCGATCGGCG
GGCTGGGCAAAGGGCATCTGGTACGCGAGGTTGATGCGTTCGACGGTGTGATCGCCCGCGCGGCCGATGCGGCGGCGATT
CATTACCGCATGCTCAACCGTTCGAAGGGCAGCGCCGTGTGGGGTCCGCGCGTACAGGCGGATCGCAAGCTCTTCCATGC
TGCGGTCCAGGCCATCGTCGCGACGCAGGAGAACCTGACTGTCGTCCCGGGCGAGGTCGCGGCGCTCCGCTTCGAAGGCG
CGAGGGTGACGGGGATCGAGCTGGCGGATGGCACTGCGCTGTCTGGGCAGGCAGTGATCCTGTGCACCGGCACCTTTCTC
GGCGGAAAGCTCTATCGCGGCGAAGAGATCATGGTCGGCGGGCGGATCGGCGAGGATTCTGCCTCGCGCCTGGCCGAACA
GATGTATGGGCTGGAGCTGCCGATGGCGCGGCTCAAGACCGGGACACCGCCGCGGCTCGACGGGCGAACGATCGACTGGG
CAGCACTGGAAGAACAGCCTTCCGACGGCGAGGACTGGACGCTGTCGGCGCTGACGCCCGCGCGGAGCAACCCCGAAATC
TTCTGCGGTATCACGCGCACCAATGCGCGAGCGCACGACATTATCCGCGCCAATCTCAACCGTTCTCCGCTGTTTTCGGG
CGCGATCGACGCGCAGGGGCCGCGCTATTGCCCCTCGATCGAGGACAAGATCCACCGGTTCGGCGATCGGGACGGGCACC
AGGTGTTCCTCGAGCCCGAAGGTCTCGACACCCACCTGGTCTATCCCAACGGCATCAGCACCTCGCTGCCGGTCGATGTG
CAACTCGGCATGTTGCGAGCCATGGACGGGTTGTTTCACGTGGAACTGGTCGAGCCGGGCTATGCGGTCGAATACGACCA
TATAGAGCCGCGTGCGCTGACCTCGGGCCTGCGGCTCAAGGATGTCGAGGGCCTCTATTGCGCCGGACAGATCAATGGCA
CCACCGGTTACGAGGAGGCGGCGGCGCAGGGATTGATCGCGGGCCTGAATGCGGCTTGCGTGGTGCTCGACCGCGAAGCA
CCCGCGCTCGACCGGGCGACCAGCTACATGGCGGTGATGGTCGACGATCTTACGTTGCACGGGGTCAGCGAGCCCTATCG
CATGCTCACCGCCCGGGCCGAGTATCGCCTGCGCCTGCGCGCCAACAATGCGACCGCGCGCCTTACGCCCCTGGCGATTG
AGCTGGGGTGTGTCGGTGGCGAGCGTGCCGAATGGTTTGCGCGGCGCGAAGAAAAGCGCGCACGTCTCGATGCGGCGCTC
GATAGTCAGGCCAGCGCCAAACAGATGCAGGATCTCGGCCTGCCGGTGAAGAGCGACTCCGGCAAGATGGCGCTGCGCGA
GTGGCTCCGCTTCCCGACCATCGACTTCCCCGATCTTGCGCCCCTGGTTCCGGAAGTGGACACAGGGACCGAGTTGGCGG
CCGAACTGGCCGAGGACGCCGCCTATGCGCCCTATCTCGCGCGGCAGGATGCCGAATTGCGCGAGTTGAAGGCCAACGAA
GCGATTACCCTGGCTGCGGATTTCCCATTTGCCGAAGTGCCTGGCCTGTCCAGCGAGATGGTCGAGCGCCTGACGGCGGC
GCGGCCGGAAACGCTCGCAGCGGCCGGGCGCGTGCCGGGCGTTACGCCAGCAGCCCTGGCGGCTGTCCTCGTCCATACGC
GCAAACGGGGCAGGGCCGCCGCGTGA

Upstream 100 bases:

>100_bases
ATGCTCGATGCGCTTTTCGGCCGCTTCTGCATCGGAAAATAGCCTGTTCCACGTGAAACACCCGCTGCAAATGGGGTAGC
GAGCGGGAGCGCTACATTGT

Downstream 100 bases:

>100_bases
TCGCAACCGAAGAGCAGGCGCGCGCATTCGTGGCGGAGCGGTGCGATGCGGCAGGGATGGAGCGTATCGAGGCACTGGTT
GCGGCGCTGAGAAGCGAGAA

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A

Number of amino acids: Translated: 621; Mature: 620

Protein sequence:

>621_residues
MGRAMHDFDILVVGGGHAGVEAACAAARMGVRTALVSFDPARIGAMSCNPAIGGLGKGHLVREVDAFDGVIARAADAAAI
HYRMLNRSKGSAVWGPRVQADRKLFHAAVQAIVATQENLTVVPGEVAALRFEGARVTGIELADGTALSGQAVILCTGTFL
GGKLYRGEEIMVGGRIGEDSASRLAEQMYGLELPMARLKTGTPPRLDGRTIDWAALEEQPSDGEDWTLSALTPARSNPEI
FCGITRTNARAHDIIRANLNRSPLFSGAIDAQGPRYCPSIEDKIHRFGDRDGHQVFLEPEGLDTHLVYPNGISTSLPVDV
QLGMLRAMDGLFHVELVEPGYAVEYDHIEPRALTSGLRLKDVEGLYCAGQINGTTGYEEAAAQGLIAGLNAACVVLDREA
PALDRATSYMAVMVDDLTLHGVSEPYRMLTARAEYRLRLRANNATARLTPLAIELGCVGGERAEWFARREEKRARLDAAL
DSQASAKQMQDLGLPVKSDSGKMALREWLRFPTIDFPDLAPLVPEVDTGTELAAELAEDAAYAPYLARQDAELRELKANE
AITLAADFPFAEVPGLSSEMVERLTAARPETLAAAGRVPGVTPAALAAVLVHTRKRGRAAA

Sequences:

>Translated_621_residues
MGRAMHDFDILVVGGGHAGVEAACAAARMGVRTALVSFDPARIGAMSCNPAIGGLGKGHLVREVDAFDGVIARAADAAAI
HYRMLNRSKGSAVWGPRVQADRKLFHAAVQAIVATQENLTVVPGEVAALRFEGARVTGIELADGTALSGQAVILCTGTFL
GGKLYRGEEIMVGGRIGEDSASRLAEQMYGLELPMARLKTGTPPRLDGRTIDWAALEEQPSDGEDWTLSALTPARSNPEI
FCGITRTNARAHDIIRANLNRSPLFSGAIDAQGPRYCPSIEDKIHRFGDRDGHQVFLEPEGLDTHLVYPNGISTSLPVDV
QLGMLRAMDGLFHVELVEPGYAVEYDHIEPRALTSGLRLKDVEGLYCAGQINGTTGYEEAAAQGLIAGLNAACVVLDREA
PALDRATSYMAVMVDDLTLHGVSEPYRMLTARAEYRLRLRANNATARLTPLAIELGCVGGERAEWFARREEKRARLDAAL
DSQASAKQMQDLGLPVKSDSGKMALREWLRFPTIDFPDLAPLVPEVDTGTELAAELAEDAAYAPYLARQDAELRELKANE
AITLAADFPFAEVPGLSSEMVERLTAARPETLAAAGRVPGVTPAALAAVLVHTRKRGRAAA
>Mature_620_residues
GRAMHDFDILVVGGGHAGVEAACAAARMGVRTALVSFDPARIGAMSCNPAIGGLGKGHLVREVDAFDGVIARAADAAAIH
YRMLNRSKGSAVWGPRVQADRKLFHAAVQAIVATQENLTVVPGEVAALRFEGARVTGIELADGTALSGQAVILCTGTFLG
GKLYRGEEIMVGGRIGEDSASRLAEQMYGLELPMARLKTGTPPRLDGRTIDWAALEEQPSDGEDWTLSALTPARSNPEIF
CGITRTNARAHDIIRANLNRSPLFSGAIDAQGPRYCPSIEDKIHRFGDRDGHQVFLEPEGLDTHLVYPNGISTSLPVDVQ
LGMLRAMDGLFHVELVEPGYAVEYDHIEPRALTSGLRLKDVEGLYCAGQINGTTGYEEAAAQGLIAGLNAACVVLDREAP
ALDRATSYMAVMVDDLTLHGVSEPYRMLTARAEYRLRLRANNATARLTPLAIELGCVGGERAEWFARREEKRARLDAALD
SQASAKQMQDLGLPVKSDSGKMALREWLRFPTIDFPDLAPLVPEVDTGTELAAELAEDAAYAPYLARQDAELRELKANEA
ITLAADFPFAEVPGLSSEMVERLTAARPETLAAAGRVPGVTPAALAAVLVHTRKRGRAAA

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family

Homologues:

Organism=Homo sapiens, GI74024895, Length=638, Percent_Identity=40.7523510971787, Blast_Score=437, Evalue=1e-122,
Organism=Homo sapiens, GI19882217, Length=663, Percent_Identity=39.2156862745098, Blast_Score=423, Evalue=1e-118,
Organism=Homo sapiens, GI183227703, Length=678, Percent_Identity=38.3480825958702, Blast_Score=417, Evalue=1e-116,
Organism=Escherichia coli, GI2367273, Length=620, Percent_Identity=46.7741935483871, Blast_Score=528, Evalue=1e-151,
Organism=Caenorhabditis elegans, GI17534255, Length=620, Percent_Identity=37.9032258064516, Blast_Score=421, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6321202, Length=620, Percent_Identity=40.1612903225806, Blast_Score=457, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24658174, Length=633, Percent_Identity=42.8120063191153, Blast_Score=440, Evalue=1e-123,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMG_ERYLH (Q2N6I8)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_459500.1
- ProteinModelPortal:   Q2N6I8
- STRING:   Q2N6I8
- GeneID:   3870233
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_13055
- NMPDR:   fig|314225.3.peg.413
- eggNOG:   COG0445
- HOGENOM:   HBG284774
- OMA:   GIQFRVL
- ProtClustDB:   PRK05192
- BioCyc:   ELIT314225:ELI_13055-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00129
- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00136

Pfam domain/function: PF01134 GIDA

EC number: NA

Molecular weight: Translated: 66485; Mature: 66354

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2; PS00606 B_KETOACYL_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRAMHDFDILVVGGGHAGVEAACAAARMGVRTALVSFDPARIGAMSCNPAIGGLGKGHL
CCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHEEECCHHHCCCEECCCCCCCCCCCCE
VREVDAFDGVIARAADAAAIHYRMLNRSKGSAVWGPRVQADRKLFHAAVQAIVATQENLT
EEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCE
VVPGEVAALRFEGARVTGIELADGTALSGQAVILCTGTFLGGKLYRGEEIMVGGRIGEDS
ECCCCEEEEEECCCEEEEEEECCCCCCCCCEEEEEECCCCCCEEECCCEEEECCCCCCHH
ASRLAEQMYGLELPMARLKTGTPPRLDGRTIDWAALEEQPSDGEDWTLSALTPARSNPEI
HHHHHHHHHCCCCCHHHHCCCCCCCCCCCEEEEHHHCCCCCCCCCEEEEEECCCCCCCCE
FCGITRTNARAHDIIRANLNRSPLFSGAIDAQGPRYCPSIEDKIHRFGDRDGHQVFLEPE
EEEEECCCCHHHHHHEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEECC
GLDTHLVYPNGISTSLPVDVQLGMLRAMDGLFHVELVEPGYAVEYDHIEPRALTSGLRLK
CCCEEEECCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCEEEECCCCCHHHHCCCEEE
DVEGLYCAGQINGTTGYEEAAAQGLIAGLNAACVVLDREAPALDRATSYMAVMVDDLTLH
CCCCEEEEEEECCCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHEEEEEEECEEEC
GVSEPYRMLTARAEYRLRLRANNATARLTPLAIELGCVGGERAEWFARREEKRARLDAAL
CCCCHHHHHHHCCEEEEEEEECCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
DSQASAKQMQDLGLPVKSDSGKMALREWLRFPTIDFPDLAPLVPEVDTGTELAAELAEDA
CCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
AYAPYLARQDAELRELKANEAITLAADFPFAEVPGLSSEMVERLTAARPETLAAAGRVPG
HCCCHHHCCCCHHHHHCCCCEEEEEECCCHHHCCCCCHHHHHHHHHCCCHHHHHCCCCCC
VTPAALAAVLVHTRKRGRAAA
CCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
GRAMHDFDILVVGGGHAGVEAACAAARMGVRTALVSFDPARIGAMSCNPAIGGLGKGHL
CCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHEEECCHHHCCCEECCCCCCCCCCCCE
VREVDAFDGVIARAADAAAIHYRMLNRSKGSAVWGPRVQADRKLFHAAVQAIVATQENLT
EEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCE
VVPGEVAALRFEGARVTGIELADGTALSGQAVILCTGTFLGGKLYRGEEIMVGGRIGEDS
ECCCCEEEEEECCCEEEEEEECCCCCCCCCEEEEEECCCCCCEEECCCEEEECCCCCCHH
ASRLAEQMYGLELPMARLKTGTPPRLDGRTIDWAALEEQPSDGEDWTLSALTPARSNPEI
HHHHHHHHHCCCCCHHHHCCCCCCCCCCCEEEEHHHCCCCCCCCCEEEEEECCCCCCCCE
FCGITRTNARAHDIIRANLNRSPLFSGAIDAQGPRYCPSIEDKIHRFGDRDGHQVFLEPE
EEEEECCCCHHHHHHEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEECC
GLDTHLVYPNGISTSLPVDVQLGMLRAMDGLFHVELVEPGYAVEYDHIEPRALTSGLRLK
CCCEEEECCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCEEEECCCCCHHHHCCCEEE
DVEGLYCAGQINGTTGYEEAAAQGLIAGLNAACVVLDREAPALDRATSYMAVMVDDLTLH
CCCCEEEEEEECCCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHEEEEEEECEEEC
GVSEPYRMLTARAEYRLRLRANNATARLTPLAIELGCVGGERAEWFARREEKRARLDAAL
CCCCHHHHHHHCCEEEEEEEECCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
DSQASAKQMQDLGLPVKSDSGKMALREWLRFPTIDFPDLAPLVPEVDTGTELAAELAEDA
CCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
AYAPYLARQDAELRELKANEAITLAADFPFAEVPGLSSEMVERLTAARPETLAAAGRVPG
HCCCHHHCCCCHHHHHCCCCEEEEEECCCHHHCCCCCHHHHHHHHHCCCHHHHHCCCCCC
VTPAALAAVLVHTRKRGRAAA
CCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA