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The map label for this gene is trmE

Identifier: 85375437

GI number: 85375437

Start: 2626584

End: 2627858

Strand: Direct

Name: trmE

Synonym: ELI_13050

Alternate gene names: 85375437

Gene position: 2626584-2627858 (Clockwise)

Preceding gene: 85375436

Following gene: 85375438

Centisome position: 86.05

GC content: 67.53

Gene sequence:

>1275_bases
GTGGAAACGATCTTCGCCCTCTCGAGCGGCGCGCCGCCCGCGGCGATCGGCGTGATACGGATCAGCGGGACCGAAGCGCG
GGGTGCGCTGGAGGCTTTGGCAGGCAGCGTGCCGGACGCGCGCAAGGCTGCGCTGCGGCGGTTGCGCGACGGCGAGGGAA
AGACGCTCGACGATGCGCTGGTGCTGTGGCTGCCGGGGCCGGACAACGCGACCGGCGAGGATTGCGTCGAGCTTCACTGC
CACGGGGGCAGGGCGGTGATCGCTGCAATCGAGCGTACACTCGGCACCATGCCGGGATTGCGCCGTGCCGAGCCCGGGGA
ATTCACGCGTCGCGCTTTTGCCAATGGCCGGATCGATCTGGCCGAGGCGGAGGGGCTGGCCGACCTGCTGTTCGCCGAAA
CCGAGCTGCAGCGGCAAGTGCTGCAGGCATCGGCGGGCGGACGGCTGTCCGAGCTGGTCGGTGGGTGGCGCGAGCGGGTG
CTGGCGCTGTCGGCGCAGGTTGAAAGTGCGCTCGATTTCTCCGACGAGGACGACGTCGACGAGCTTGCACCCGCCTTCTA
CACCGATGTTTCCACACTGGCGGACGAACTTGGCGAATGGCTCGAGCGCCCGCAGGTCGAACGGCTCCGCGACGGAGTAC
GCGTGGTGTTCGCCGGTCCGCCTAACGCCGGCAAGTCGACGCTTTTCAATGCATTGCTGCAGAGCGAAGCGGCGATCGTC
TCGCCGATCGCCGGTACGACCCGCGACGTGCTAGAACGCCCGGTTGCGTTTGGCGGCACCCCCTTCACCCTGATCGACAC
CGCCGGTCTGCACGAAGGTGGCGATGACAGTATTGAAAGGATCGGCATCGACCGCGCGCGTGCGGCTTTGCGCGATGCCG
ATATCGTGCTGTGGCTCGGGCCCGAAGGCGAAGGGCCCGACAATGCGTGGGAGATCGACGCGCAGAGCGATATCGACGAA
CGCACCGCCAAATCCGCGCCACGGCTACGATTGTCGGCGAAGACAGGGGAGGGGATCGATGCGCTCGTTCGCGACCTGCG
CGATGCCGCGCGCGACCTGCTTCCCAGGCCCGGCGACGTGGCGGTCAATGCGCGGCAGCACGCTCTGCTGTCCGAAGCGG
CTGGCGAACTCGCGGAAATCACGCGCGGGCAGGACTTGCTGATCACAGCCGAGCACCTCCGCACCGCTCGCAGCGCCTTC
GACCGCTTCACCGGCCGCGCGACCACCGAAGATATGCTCGATGCGCTTTTCGGCCGCTTCTGCATCGGAAAATAG

Upstream 100 bases:

>100_bases
GCTGTTCCAGAACTTCGTCGAAGGCAGTCTGGCCGCCGGCGGCATGCGGGCCGCAGGTTCGTCGAAGAAGTCCAAGGACT
GAACGCCCCACCTGTTTCAC

Downstream 100 bases:

>100_bases
CCTGTTCCACGTGAAACACCCGCTGCAAATGGGGTAGCGAGCGGGAGCGCTACATTGTATGGGCCGCGCCATGCACGATT
TCGACATCCTTGTGGTCGGC

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
METIFALSSGAPPAAIGVIRISGTEARGALEALAGSVPDARKAALRRLRDGEGKTLDDALVLWLPGPDNATGEDCVELHC
HGGRAVIAAIERTLGTMPGLRRAEPGEFTRRAFANGRIDLAEAEGLADLLFAETELQRQVLQASAGGRLSELVGGWRERV
LALSAQVESALDFSDEDDVDELAPAFYTDVSTLADELGEWLERPQVERLRDGVRVVFAGPPNAGKSTLFNALLQSEAAIV
SPIAGTTRDVLERPVAFGGTPFTLIDTAGLHEGGDDSIERIGIDRARAALRDADIVLWLGPEGEGPDNAWEIDAQSDIDE
RTAKSAPRLRLSAKTGEGIDALVRDLRDAARDLLPRPGDVAVNARQHALLSEAAGELAEITRGQDLLITAEHLRTARSAF
DRFTGRATTEDMLDALFGRFCIGK

Sequences:

>Translated_424_residues
METIFALSSGAPPAAIGVIRISGTEARGALEALAGSVPDARKAALRRLRDGEGKTLDDALVLWLPGPDNATGEDCVELHC
HGGRAVIAAIERTLGTMPGLRRAEPGEFTRRAFANGRIDLAEAEGLADLLFAETELQRQVLQASAGGRLSELVGGWRERV
LALSAQVESALDFSDEDDVDELAPAFYTDVSTLADELGEWLERPQVERLRDGVRVVFAGPPNAGKSTLFNALLQSEAAIV
SPIAGTTRDVLERPVAFGGTPFTLIDTAGLHEGGDDSIERIGIDRARAALRDADIVLWLGPEGEGPDNAWEIDAQSDIDE
RTAKSAPRLRLSAKTGEGIDALVRDLRDAARDLLPRPGDVAVNARQHALLSEAAGELAEITRGQDLLITAEHLRTARSAF
DRFTGRATTEDMLDALFGRFCIGK
>Mature_424_residues
METIFALSSGAPPAAIGVIRISGTEARGALEALAGSVPDARKAALRRLRDGEGKTLDDALVLWLPGPDNATGEDCVELHC
HGGRAVIAAIERTLGTMPGLRRAEPGEFTRRAFANGRIDLAEAEGLADLLFAETELQRQVLQASAGGRLSELVGGWRERV
LALSAQVESALDFSDEDDVDELAPAFYTDVSTLADELGEWLERPQVERLRDGVRVVFAGPPNAGKSTLFNALLQSEAAIV
SPIAGTTRDVLERPVAFGGTPFTLIDTAGLHEGGDDSIERIGIDRARAALRDADIVLWLGPEGEGPDNAWEIDAQSDIDE
RTAKSAPRLRLSAKTGEGIDALVRDLRDAARDLLPRPGDVAVNARQHALLSEAAGELAEITRGQDLLITAEHLRTARSAF
DRFTGRATTEDMLDALFGRFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=462, Percent_Identity=42.2077922077922, Blast_Score=281, Evalue=6e-76,
Organism=Homo sapiens, GI193082993, Length=443, Percent_Identity=43.3408577878104, Blast_Score=281, Evalue=1e-75,
Organism=Homo sapiens, GI193082988, Length=494, Percent_Identity=39.6761133603239, Blast_Score=270, Evalue=2e-72,
Organism=Escherichia coli, GI2367268, Length=455, Percent_Identity=37.5824175824176, Blast_Score=240, Evalue=1e-64,
Organism=Escherichia coli, GI87082120, Length=95, Percent_Identity=35.7894736842105, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17507259, Length=449, Percent_Identity=32.293986636971, Blast_Score=201, Evalue=7e-52,
Organism=Saccharomyces cerevisiae, GI6323665, Length=491, Percent_Identity=33.1975560081466, Blast_Score=239, Evalue=6e-64,
Organism=Drosophila melanogaster, GI45550813, Length=303, Percent_Identity=43.5643564356436, Blast_Score=236, Evalue=3e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_ERYLH (Q2N6I9)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_459499.1
- ProteinModelPortal:   Q2N6I9
- STRING:   Q2N6I9
- GeneID:   3870232
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_13050
- NMPDR:   fig|314225.3.peg.414
- eggNOG:   COG0486
- HOGENOM:   HBG746795
- OMA:   LIDNAMV
- PhylomeDB:   Q2N6I9
- ProtClustDB:   PRK05291
- BioCyc:   ELIT314225:ELI_13050-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- PRINTS:   PR00326
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 45308; Mature: 45308

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: NA

Important sites: BINDING 20-20 BINDING 77-77 BINDING 117-117 BINDING 424-424

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METIFALSSGAPPAAIGVIRISGTEARGALEALAGSVPDARKAALRRLRDGEGKTLDDAL
CCCEEEECCCCCCCEEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEECCEE
VLWLPGPDNATGEDCVELHCHGGRAVIAAIERTLGTMPGLRRAEPGEFTRRAFANGRIDL
EEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCEEE
AEAEGLADLLFAETELQRQVLQASAGGRLSELVGGWRERVLALSAQVESALDFSDEDDVD
HHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
ELAPAFYTDVSTLADELGEWLERPQVERLRDGVRVVFAGPPNAGKSTLFNALLQSEAAIV
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCHHHH
SPIAGTTRDVLERPVAFGGTPFTLIDTAGLHEGGDDSIERIGIDRARAALRDADIVLWLG
CCCCCCHHHHHHCCHHCCCCCEEEEECCCCCCCCCHHHHHHCHHHHHHHHCCCCEEEEEC
PEGEGPDNAWEIDAQSDIDERTAKSAPRLRLSAKTGEGIDALVRDLRDAARDLLPRPGDV
CCCCCCCCCEEECCCCCHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCE
AVNARQHALLSEAAGELAEITRGQDLLITAEHLRTARSAFDRFTGRATTEDMLDALFGRF
EECHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
CIGK
HCCC
>Mature Secondary Structure
METIFALSSGAPPAAIGVIRISGTEARGALEALAGSVPDARKAALRRLRDGEGKTLDDAL
CCCEEEECCCCCCCEEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEECCEE
VLWLPGPDNATGEDCVELHCHGGRAVIAAIERTLGTMPGLRRAEPGEFTRRAFANGRIDL
EEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCEEE
AEAEGLADLLFAETELQRQVLQASAGGRLSELVGGWRERVLALSAQVESALDFSDEDDVD
HHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
ELAPAFYTDVSTLADELGEWLERPQVERLRDGVRVVFAGPPNAGKSTLFNALLQSEAAIV
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCHHHH
SPIAGTTRDVLERPVAFGGTPFTLIDTAGLHEGGDDSIERIGIDRARAALRDADIVLWLG
CCCCCCHHHHHHCCHHCCCCCEEEEECCCCCCCCCHHHHHHCHHHHHHHHCCCCEEEEEC
PEGEGPDNAWEIDAQSDIDERTAKSAPRLRLSAKTGEGIDALVRDLRDAARDLLPRPGDV
CCCCCCCCCEEECCCCCHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCE
AVNARQHALLSEAAGELAEITRGQDLLITAEHLRTARSAFDRFTGRATTEDMLDALFGRF
EECHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
CIGK
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA