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The map label for this gene is xthA [H]

Identifier: 85375417

GI number: 85375417

Start: 2610739

End: 2611527

Strand: Direct

Name: xthA [H]

Synonym: ELI_12950

Alternate gene names: 85375417

Gene position: 2610739-2611527 (Clockwise)

Preceding gene: 85375416

Following gene: 85375428

Centisome position: 85.53

GC content: 64.13

Gene sequence:

>789_bases
GTGAGAATTGCCACTTTCAATATCAACGGCATCAAGGCCCGCCTGCCGCGCCTCATCGAATGGCTCGAGGAAACGCGGCC
GACGGTCGCCTGCCTTCAGGAAATCAAGAGCCAGGACGACGGCTTTCCCGCCGACGAATTCGAGAAGATCGGCTATCACG
CCATCTGGCACGGGCAGAAAAGCTTCAACGGCGTCGCCATCCTGGCCGACGGGGTGAAGCCGGAAGAGACCCAGCGCGGA
CTCGGCGTGGACGGGCCCAAGGAAGGCGAGGGCGAGCAGGCGCGTTATCTCGAGGCCCACGTGAACGGCGTGCGCATCGC
TTGCCTTTACCTGCCCAACGGCAATCCGCATCCCGGGCCAAAATTCGACTACAAGCTCGCTTGGATGGAAAAGCTGCGCG
CCCGCATGCGCGAATTGTGGCAGAGCGAACAGCCGACGATCGTCACCGGCGATTTCAACGTCATCCCGCATGACGACGAT
GTCTGGTCGCCCAAGGCGATGCAGGACGATGCGCTGATGCAGCCGGAATCGCGCGATGCCTACCAGCGTCAGCTGGCCGA
CGGCTGGACCGATGCGGTCCGCACACTCAACCCGCGCGGCGGCGTGTGGACCTTTTGGGATTACCAGCGCGGTGCCTGGC
AGCGCGACCACGGCTTCCGCATCGACCATTGCCTGCTGTCGCCCGAACTGGCCGATCGCCTGACCGCAGTCGGCGTCGAC
AAGGACTATCGCGGCCGCGAGAAGGCCAGCGATCACACGCCGGTCTGGGTCGAACTTGCCGACGGCTGA

Upstream 100 bases:

>100_bases
GAAGGGCCGTACTTTCTTTTAGCGCAGTACCCGAAGGTACGAGCATGGCTTCGACAACCCCGGATCGGGTCCGGGGCCGA
ACCGTCCCGGGGGTGAGAGC

Downstream 100 bases:

>100_bases
CGACGGGCAATAGAAAACCCCTCCGCATCCCGTCGGACACGAAGAGGTTCCCTGCGACCCTGTCCTGCCGGAGCGTGTCC
GGGCAGGGCGGGCTGACTGA

Product: hypothetical protein

Products: NA

Alternate protein names: EXO III; Exonuclease III [H]

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MRIATFNINGIKARLPRLIEWLEETRPTVACLQEIKSQDDGFPADEFEKIGYHAIWHGQKSFNGVAILADGVKPEETQRG
LGVDGPKEGEGEQARYLEAHVNGVRIACLYLPNGNPHPGPKFDYKLAWMEKLRARMRELWQSEQPTIVTGDFNVIPHDDD
VWSPKAMQDDALMQPESRDAYQRQLADGWTDAVRTLNPRGGVWTFWDYQRGAWQRDHGFRIDHCLLSPELADRLTAVGVD
KDYRGREKASDHTPVWVELADG

Sequences:

>Translated_262_residues
MRIATFNINGIKARLPRLIEWLEETRPTVACLQEIKSQDDGFPADEFEKIGYHAIWHGQKSFNGVAILADGVKPEETQRG
LGVDGPKEGEGEQARYLEAHVNGVRIACLYLPNGNPHPGPKFDYKLAWMEKLRARMRELWQSEQPTIVTGDFNVIPHDDD
VWSPKAMQDDALMQPESRDAYQRQLADGWTDAVRTLNPRGGVWTFWDYQRGAWQRDHGFRIDHCLLSPELADRLTAVGVD
KDYRGREKASDHTPVWVELADG
>Mature_262_residues
MRIATFNINGIKARLPRLIEWLEETRPTVACLQEIKSQDDGFPADEFEKIGYHAIWHGQKSFNGVAILADGVKPEETQRG
LGVDGPKEGEGEQARYLEAHVNGVRIACLYLPNGNPHPGPKFDYKLAWMEKLRARMRELWQSEQPTIVTGDFNVIPHDDD
VWSPKAMQDDALMQPESRDAYQRQLADGWTDAVRTLNPRGGVWTFWDYQRGAWQRDHGFRIDHCLLSPELADRLTAVGVD
KDYRGREKASDHTPVWVELADG

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=267, Percent_Identity=27.7153558052434, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI18375503, Length=267, Percent_Identity=27.7153558052434, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI18375501, Length=267, Percent_Identity=27.7153558052434, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI1788046, Length=271, Percent_Identity=32.1033210332103, Blast_Score=131, Evalue=4e-32,
Organism=Drosophila melanogaster, GI221330655, Length=263, Percent_Identity=28.1368821292776, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI17136678, Length=263, Percent_Identity=28.1368821292776, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29863; Mature: 29863

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIATFNINGIKARLPRLIEWLEETRPTVACLQEIKSQDDGFPADEFEKIGYHAIWHGQK
CEEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHCCCEEEECCCC
SFNGVAILADGVKPEETQRGLGVDGPKEGEGEQARYLEAHVNGVRIACLYLPNGNPHPGP
CCCCEEEEECCCCCHHHHCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEECCCCCCCCCC
KFDYKLAWMEKLRARMRELWQSEQPTIVTGDFNVIPHDDDVWSPKAMQDDALMQPESRDA
CCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCEEECCCCCCCCCCCCCCCCCCCCCCHHH
YQRQLADGWTDAVRTLNPRGGVWTFWDYQRGAWQRDHGFRIDHCLLSPELADRLTAVGVD
HHHHHHCCHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCHHHEECCHHHHHHHHHCCCC
KDYRGREKASDHTPVWVELADG
CCCCCCCCCCCCCCEEEEECCC
>Mature Secondary Structure
MRIATFNINGIKARLPRLIEWLEETRPTVACLQEIKSQDDGFPADEFEKIGYHAIWHGQK
CEEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHCCCEEEECCCC
SFNGVAILADGVKPEETQRGLGVDGPKEGEGEQARYLEAHVNGVRIACLYLPNGNPHPGP
CCCCEEEEECCCCCHHHHCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEECCCCCCCCCC
KFDYKLAWMEKLRARMRELWQSEQPTIVTGDFNVIPHDDDVWSPKAMQDDALMQPESRDA
CCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCEEECCCCCCCCCCCCCCCCCCCCCCHHH
YQRQLADGWTDAVRTLNPRGGVWTFWDYQRGAWQRDHGFRIDHCLLSPELADRLTAVGVD
HHHHHHCCHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCHHHEECCHHHHHHHHHCCCC
KDYRGREKASDHTPVWVELADG
CCCCCCCCCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]