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The map label for this gene is yngI [H]

Identifier: 85375292

GI number: 85375292

Start: 2489114

End: 2490652

Strand: Reverse

Name: yngI [H]

Synonym: ELI_12325

Alternate gene names: 85375292

Gene position: 2490652-2489114 (Counterclockwise)

Preceding gene: 85375293

Following gene: 85375291

Centisome position: 81.6

GC content: 63.29

Gene sequence:

>1539_bases
ATGCATCCGATTCAACATGCCGCCGCGCGCCCCGATCATCCGGCCGTCATTATGGCGGGAACCGGAGAGACCGTTACTTT
CGGCGAGATGGACGCAGCCTCGAACCGGTTTGCGCATTTGCTGCGCGCACGGGGCATCGGCCATGACGAGGCCTTCGCCG
TCCTGCTGGAGAACCGGGCCGAGTTCTACACGCTGATCTGGGGCTCGCAGCGCGCCGGCACGATGCTGGTGCCGATCTCC
ACCCGGCTGACCGCGCCCGAGATCGCCTATATCCTCAAAGACAGCGAAGCCAAGCTGCTGATCACATCGACGGCGTTCGA
CGATGTGATGCAGGGCATTCGTGCGGAATGCTCCGACCTGCCAGTTTTGATGATGGACGGCGAAGGCGACGAGGATTTCG
CCGCCGCGCTCGACGCGCAGCCATCCGAGCCGATCGACGACCAGCGCGCCGGGTTGGTGATGCTGTATTCCTCCGGCACC
ACCGGGCGCCCCAAGGGCATCCGGCCCGCGCCGCCGGAAGATCCCGATCCGCTGGCGGCCAATCCGTTGATGGGCCTCGC
GATCATGGGGGCGGGGATGCCGGCGGACGGCAGCATGGTCTACTTGTCGCCCGCGCCGCTCTATCATGCAGCGCCGATCG
GCTGGTCCTCCACCGTCCATCGCCTTGGCGGCACGGTGGTGATGATGGCCAAGTTCGATCCCGAGACCGCGCTCGAAGCG
ATCGAGAAGTACAAGGTCACCGACAGCCAGTGGGTACCGACGCATTTCGTGCGCATGCTCAAGCTGCCCGAGGAAATCCG
CACCAGATACGATCTCTCCAGCCACCAGCGCGCGCTCCATGCTGCCGCACCCTGCCCGGTGCCGATCAAGAAGCAGATGA
TCGAATGGTGGGGGCCGATCGTGAACGAATACTATGCCGGCTCGGAAGGGATCGGCATGACGCTGGTGAAGAGCGAGGAC
TGGTTGACCCATCCGGGCACTGTCGGTCGCGCGATCCACGGCACGCTCCACATCTGCGGGCCGGACGGGGAGGAAGTGCC
GGCAGGGACGGACGGGCTGATCTTTTTCGAAAACGATATCCTGCCGACCTATCACAACGATCCGGACAAGACGAAGGATG
CCATGCATCCCAAGGGGTGGATGACGCTGGGCGATATCGGCCATGTGGACGAGGACGGATTTCTCTATCTGACCGACCGC
AAGAGCCACATGATCATCAGCGGCGGCGTCAACATCTACCCGCAGGAGATCGAGAACCTGCTGATCAGCCATCCCAAGGT
GATGGACGCCGCCGTGATCGGCGCGCCGGACCCGGACCTCGGTGAAAAAGTCGTGGCCGTGGTGCAGCCCAAGGACATGA
GCGATGCGGGAGAGGCGTTCGAAGGCGAATTGCGCGATTATCTCGCGCCCAACCTCTCCCGGATCAAGATGCCCCGGCTG
TTCGATTTCCGTCCCGATCTGCCGCGCGAAGCCAATGGCAAACTCTACAAGCGCGAATTGCGGGATGAATTCGCGGCCAA
GGCCGAGGAGTCAACATGA

Upstream 100 bases:

>100_bases
CCGCCGCCTTTTTTGTGTTCGCAGGCGCAATCCGGCGGCGGAATCTCCTTGCCCGAAATCGGTTGCTGGATAAGACTTTC
TCTCATGGGAGAGAAAACAC

Downstream 100 bases:

>100_bases
TGGCGAGCGGAACGCCGATCCTGCCGCCTGACACTGCCCGTGAAGTGATCGATCCGCACAGCTACGCCGAGTGGGGGTCG
CTGCTCGACACATTCGACAG

Product: long-chain-fatty-acid--CoA ligase

Products: AMP; diphosphate; acyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MHPIQHAAARPDHPAVIMAGTGETVTFGEMDAASNRFAHLLRARGIGHDEAFAVLLENRAEFYTLIWGSQRAGTMLVPIS
TRLTAPEIAYILKDSEAKLLITSTAFDDVMQGIRAECSDLPVLMMDGEGDEDFAAALDAQPSEPIDDQRAGLVMLYSSGT
TGRPKGIRPAPPEDPDPLAANPLMGLAIMGAGMPADGSMVYLSPAPLYHAAPIGWSSTVHRLGGTVVMMAKFDPETALEA
IEKYKVTDSQWVPTHFVRMLKLPEEIRTRYDLSSHQRALHAAAPCPVPIKKQMIEWWGPIVNEYYAGSEGIGMTLVKSED
WLTHPGTVGRAIHGTLHICGPDGEEVPAGTDGLIFFENDILPTYHNDPDKTKDAMHPKGWMTLGDIGHVDEDGFLYLTDR
KSHMIISGGVNIYPQEIENLLISHPKVMDAAVIGAPDPDLGEKVVAVVQPKDMSDAGEAFEGELRDYLAPNLSRIKMPRL
FDFRPDLPREANGKLYKRELRDEFAAKAEEST

Sequences:

>Translated_512_residues
MHPIQHAAARPDHPAVIMAGTGETVTFGEMDAASNRFAHLLRARGIGHDEAFAVLLENRAEFYTLIWGSQRAGTMLVPIS
TRLTAPEIAYILKDSEAKLLITSTAFDDVMQGIRAECSDLPVLMMDGEGDEDFAAALDAQPSEPIDDQRAGLVMLYSSGT
TGRPKGIRPAPPEDPDPLAANPLMGLAIMGAGMPADGSMVYLSPAPLYHAAPIGWSSTVHRLGGTVVMMAKFDPETALEA
IEKYKVTDSQWVPTHFVRMLKLPEEIRTRYDLSSHQRALHAAAPCPVPIKKQMIEWWGPIVNEYYAGSEGIGMTLVKSED
WLTHPGTVGRAIHGTLHICGPDGEEVPAGTDGLIFFENDILPTYHNDPDKTKDAMHPKGWMTLGDIGHVDEDGFLYLTDR
KSHMIISGGVNIYPQEIENLLISHPKVMDAAVIGAPDPDLGEKVVAVVQPKDMSDAGEAFEGELRDYLAPNLSRIKMPRL
FDFRPDLPREANGKLYKRELRDEFAAKAEEST
>Mature_512_residues
MHPIQHAAARPDHPAVIMAGTGETVTFGEMDAASNRFAHLLRARGIGHDEAFAVLLENRAEFYTLIWGSQRAGTMLVPIS
TRLTAPEIAYILKDSEAKLLITSTAFDDVMQGIRAECSDLPVLMMDGEGDEDFAAALDAQPSEPIDDQRAGLVMLYSSGT
TGRPKGIRPAPPEDPDPLAANPLMGLAIMGAGMPADGSMVYLSPAPLYHAAPIGWSSTVHRLGGTVVMMAKFDPETALEA
IEKYKVTDSQWVPTHFVRMLKLPEEIRTRYDLSSHQRALHAAAPCPVPIKKQMIEWWGPIVNEYYAGSEGIGMTLVKSED
WLTHPGTVGRAIHGTLHICGPDGEEVPAGTDGLIFFENDILPTYHNDPDKTKDAMHPKGWMTLGDIGHVDEDGFLYLTDR
KSHMIISGGVNIYPQEIENLLISHPKVMDAAVIGAPDPDLGEKVVAVVQPKDMSDAGEAFEGELRDYLAPNLSRIKMPRL
FDFRPDLPREANGKLYKRELRDEFAAKAEEST

Specific function: Could Catalyze The Transfer Of CoA To Crotonobetaine Or Carnitine. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI42544132, Length=532, Percent_Identity=24.6240601503759, Blast_Score=144, Evalue=3e-34,
Organism=Homo sapiens, GI157311624, Length=541, Percent_Identity=26.8022181146026, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI157311622, Length=541, Percent_Identity=26.8022181146026, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI58082049, Length=538, Percent_Identity=26.9516728624535, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI38505220, Length=531, Percent_Identity=25.8003766478343, Blast_Score=136, Evalue=6e-32,
Organism=Homo sapiens, GI122937307, Length=533, Percent_Identity=26.4540337711069, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI115511026, Length=516, Percent_Identity=25.5813953488372, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI156151445, Length=534, Percent_Identity=24.9063670411985, Blast_Score=129, Evalue=7e-30,
Organism=Homo sapiens, GI187761345, Length=531, Percent_Identity=25.2354048964218, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI187761343, Length=531, Percent_Identity=25.2354048964218, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI40807357, Length=543, Percent_Identity=25.2302025782689, Blast_Score=93, Evalue=7e-19,
Organism=Homo sapiens, GI38524616, Length=547, Percent_Identity=25.7769652650823, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI8923896, Length=568, Percent_Identity=22.0070422535211, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI253970489, Length=386, Percent_Identity=24.0932642487047, Blast_Score=73, Evalue=8e-13,
Organism=Homo sapiens, GI13375727, Length=407, Percent_Identity=24.3243243243243, Blast_Score=66, Evalue=9e-11,
Organism=Escherichia coli, GI221142682, Length=513, Percent_Identity=26.9005847953216, Blast_Score=156, Evalue=3e-39,
Organism=Escherichia coli, GI1788107, Length=552, Percent_Identity=26.4492753623188, Blast_Score=155, Evalue=7e-39,
Organism=Escherichia coli, GI145693145, Length=521, Percent_Identity=24.3761996161228, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1786810, Length=523, Percent_Identity=24.8565965583174, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1790505, Length=551, Percent_Identity=20.6896551724138, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1786529, Length=533, Percent_Identity=22.1388367729831, Blast_Score=62, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17558820, Length=516, Percent_Identity=27.3255813953488, Blast_Score=161, Evalue=6e-40,
Organism=Caenorhabditis elegans, GI17559526, Length=515, Percent_Identity=28.9320388349515, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI32563687, Length=517, Percent_Identity=24.9516441005803, Blast_Score=147, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI17531443, Length=531, Percent_Identity=25.9887005649718, Blast_Score=145, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI71994703, Length=543, Percent_Identity=24.8618784530387, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI71994694, Length=543, Percent_Identity=24.8618784530387, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI71994690, Length=543, Percent_Identity=24.8618784530387, Blast_Score=135, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI17557194, Length=529, Percent_Identity=25.8979206049149, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17560308, Length=372, Percent_Identity=28.494623655914, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17538037, Length=530, Percent_Identity=24.7169811320755, Blast_Score=101, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17551278, Length=568, Percent_Identity=23.943661971831, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI115534440, Length=552, Percent_Identity=23.731884057971, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17560140, Length=514, Percent_Identity=23.5408560311284, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71985884, Length=154, Percent_Identity=30.5194805194805, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6319699, Length=375, Percent_Identity=27.4666666666667, Blast_Score=114, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6319264, Length=578, Percent_Identity=22.1453287197232, Blast_Score=80, Evalue=6e-16,
Organism=Drosophila melanogaster, GI21355181, Length=518, Percent_Identity=28.3783783783784, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24581924, Length=543, Percent_Identity=24.4935543278085, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI18859661, Length=533, Percent_Identity=25.515947467167, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI21358303, Length=319, Percent_Identity=26.6457680250784, Blast_Score=112, Evalue=7e-25,
Organism=Drosophila melanogaster, GI24648253, Length=508, Percent_Identity=23.6220472440945, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24648255, Length=508, Percent_Identity=23.8188976377953, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24653035, Length=529, Percent_Identity=23.062381852552, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI281365686, Length=317, Percent_Identity=24.6056782334385, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI161076582, Length=536, Percent_Identity=23.134328358209, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24648260, Length=518, Percent_Identity=24.1312741312741, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24656500, Length=514, Percent_Identity=22.1789883268482, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI21356441, Length=527, Percent_Identity=21.6318785578748, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21356947, Length=518, Percent_Identity=22.2007722007722, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI19922652, Length=534, Percent_Identity=20.7865168539326, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI20130357, Length=370, Percent_Identity=26.2162162162162, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24648257, Length=510, Percent_Identity=23.1372549019608, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI62472339, Length=563, Percent_Identity=21.8472468916519, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24667955, Length=544, Percent_Identity=21.875, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24667959, Length=380, Percent_Identity=23.6842105263158, Blast_Score=83, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3

Molecular weight: Translated: 56021; Mature: 56021

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHPIQHAAARPDHPAVIMAGTGETVTFGEMDAASNRFAHLLRARGIGHDEAFAVLLENRA
CCCCCCCCCCCCCCEEEEECCCCEEEECCCCHHHHHHHHHHHHCCCCCHHHHEEEECCCC
EFYTLIWGSQRAGTMLVPISTRLTAPEIAYILKDSEAKLLITSTAFDDVMQGIRAECSDL
EEEEEEECCCCCCEEEEEECCCCCCCCEEEEEECCCCEEEEEECHHHHHHHHHHHHHCCC
PVLMMDGEGDEDFAAALDAQPSEPIDDQRAGLVMLYSSGTTGRPKGIRPAPPEDPDPLAA
CEEEECCCCCHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCC
NPLMGLAIMGAGMPADGSMVYLSPAPLYHAAPIGWSSTVHRLGGTVVMMAKFDPETALEA
CCHHEEHEEECCCCCCCCEEEECCCCCCCCCCCCHHHHHHHCCCCEEEEEECCHHHHHHH
IEKYKVTDSQWVPTHFVRMLKLPEEIRTRYDLSSHQRALHAAAPCPVPIKKQMIEWWGPI
HHHHCCCCCCCCHHHHHHHHHCCHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHH
VNEYYAGSEGIGMTLVKSEDWLTHPGTVGRAIHGTLHICGPDGEEVPAGTDGLIFFENDI
HHHHHCCCCCCCEEEEECCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCEEEEECCC
LPTYHNDPDKTKDAMHPKGWMTLGDIGHVDEDGFLYLTDRKSHMIISGGVNIYPQEIENL
CCCCCCCCCCHHHCCCCCCCEEECCCCCCCCCCEEEEECCCCEEEEECCCEECHHHHHHH
LISHPKVMDAAVIGAPDPDLGEKVVAVVQPKDMSDAGEAFEGELRDYLAPNLSRIKMPRL
HHCCCCHHHEEEECCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHCCCCCE
FDFRPDLPREANGKLYKRELRDEFAAKAEEST
ECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MHPIQHAAARPDHPAVIMAGTGETVTFGEMDAASNRFAHLLRARGIGHDEAFAVLLENRA
CCCCCCCCCCCCCCEEEEECCCCEEEECCCCHHHHHHHHHHHHCCCCCHHHHEEEECCCC
EFYTLIWGSQRAGTMLVPISTRLTAPEIAYILKDSEAKLLITSTAFDDVMQGIRAECSDL
EEEEEEECCCCCCEEEEEECCCCCCCCEEEEEECCCCEEEEEECHHHHHHHHHHHHHCCC
PVLMMDGEGDEDFAAALDAQPSEPIDDQRAGLVMLYSSGTTGRPKGIRPAPPEDPDPLAA
CEEEECCCCCHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCC
NPLMGLAIMGAGMPADGSMVYLSPAPLYHAAPIGWSSTVHRLGGTVVMMAKFDPETALEA
CCHHEEHEEECCCCCCCCEEEECCCCCCCCCCCCHHHHHHHCCCCEEEEEECCHHHHHHH
IEKYKVTDSQWVPTHFVRMLKLPEEIRTRYDLSSHQRALHAAAPCPVPIKKQMIEWWGPI
HHHHCCCCCCCCHHHHHHHHHCCHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHH
VNEYYAGSEGIGMTLVKSEDWLTHPGTVGRAIHGTLHICGPDGEEVPAGTDGLIFFENDI
HHHHHCCCCCCCEEEEECCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCEEEEECCC
LPTYHNDPDKTKDAMHPKGWMTLGDIGHVDEDGFLYLTDRKSHMIISGGVNIYPQEIENL
CCCCCCCCCCHHHCCCCCCCEEECCCCCCCCCCEEEEECCCCEEEEECCCEECHHHHHHH
LISHPKVMDAAVIGAPDPDLGEKVVAVVQPKDMSDAGEAFEGELRDYLAPNLSRIKMPRL
HHCCCCHHHEEEECCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHCCCCCE
FDFRPDLPREANGKLYKRELRDEFAAKAEEST
ECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; long-chain carboxylic acid; CoA

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]