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The map label for this gene is xthA [H]

Identifier: 85375221

GI number: 85375221

Start: 2415703

End: 2416500

Strand: Direct

Name: xthA [H]

Synonym: ELI_11970

Alternate gene names: 85375221

Gene position: 2415703-2416500 (Clockwise)

Preceding gene: 85375219

Following gene: 85375222

Centisome position: 79.14

GC content: 60.9

Gene sequence:

>798_bases
ATGGTTTCCGTCACCACCTGGAATATCAATTCCGTCCGCCTGCGCATGCCGATCGTCGAGCGATTCATCGACCAGGAAGC
GCCGGACGTGCTGTGCCTGCAGGAGATCAAGTGCCAGGAACACCAGTTTCCCTACGAGGCCTTTCGCAAGCTTGGCTACG
AGCATTTCGCGGTGCACGGGCAGAAGGGCTATCACGGCGTGGCGACGGTCGGGAAGGTCCCCTTCACCGAATTCTCCCGC
CATGACTGGCAGGACAATGGCGAAGCGCGCCATGTCGGGATCGAACTGACCGAAGGCCCAGCAAAGGGCACGGTGATCGA
GAATGTTTACGTCCCCGCCGGCGGCGATATTCCCGACCGCGAGCAGAACCTAAAATTCGGCCAGAAACTCGATTTCCTCG
AACGGATGACGCGCTGGGCGGACAAGGTCGATCGGCCGACGCTGATCGTCGGTGATTTCAACATCGCACCGCTGGAAAGC
GATGTCTGGAACCACAAGCAATTGCTCAAGGTCGTCAGCCACACGCCAGTCGAGGTCGAAACGCTGCAACGCTTCATGGA
CGCGCATGGCTGGAGCGATATCGGCCGCGAACACATCAGGGCGCCGGAACGTTATTACAGCTGGTGGAGCTATCGCTCGC
CCGACTGGCGCAAGAACGATCGCGGGCGGCGGCTCGATCATATGTGGGCGAGCCCGGAACTGGCGGCGCAGGCGACCGGG
CACCGGTTGCTCGAAGATGCTCGCAGCTGGGAGAAGCCGTCCGATCATATCCCGCTGACGACGGAGTTCACCTTCTGA

Upstream 100 bases:

>100_bases
GGATCGTGTCGCCGTGCTATCGCCCTTCGTTGTGACAGCAAAGCGAAAACCCGCCCGCGCTCCCTTGCTTGGGCGGGGCG
CGCTACCTAAGGCAAGCGCC

Downstream 100 bases:

>100_bases
ACGGGGCCGGGCCTTCTCCTACCCGCCGCGTGGCACAGGCGATCGACGGGCTTCGCCACGGCTGGCCGATCGCGCTGGCG
AAGGCGGGCACGCTGTTGCC

Product: exonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III [H]

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MVSVTTWNINSVRLRMPIVERFIDQEAPDVLCLQEIKCQEHQFPYEAFRKLGYEHFAVHGQKGYHGVATVGKVPFTEFSR
HDWQDNGEARHVGIELTEGPAKGTVIENVYVPAGGDIPDREQNLKFGQKLDFLERMTRWADKVDRPTLIVGDFNIAPLES
DVWNHKQLLKVVSHTPVEVETLQRFMDAHGWSDIGREHIRAPERYYSWWSYRSPDWRKNDRGRRLDHMWASPELAAQATG
HRLLEDARSWEKPSDHIPLTTEFTF

Sequences:

>Translated_265_residues
MVSVTTWNINSVRLRMPIVERFIDQEAPDVLCLQEIKCQEHQFPYEAFRKLGYEHFAVHGQKGYHGVATVGKVPFTEFSR
HDWQDNGEARHVGIELTEGPAKGTVIENVYVPAGGDIPDREQNLKFGQKLDFLERMTRWADKVDRPTLIVGDFNIAPLES
DVWNHKQLLKVVSHTPVEVETLQRFMDAHGWSDIGREHIRAPERYYSWWSYRSPDWRKNDRGRRLDHMWASPELAAQATG
HRLLEDARSWEKPSDHIPLTTEFTF
>Mature_265_residues
MVSVTTWNINSVRLRMPIVERFIDQEAPDVLCLQEIKCQEHQFPYEAFRKLGYEHFAVHGQKGYHGVATVGKVPFTEFSR
HDWQDNGEARHVGIELTEGPAKGTVIENVYVPAGGDIPDREQNLKFGQKLDFLERMTRWADKVDRPTLIVGDFNIAPLES
DVWNHKQLLKVVSHTPVEVETLQRFMDAHGWSDIGREHIRAPERYYSWWSYRSPDWRKNDRGRRLDHMWASPELAAQATG
HRLLEDARSWEKPSDHIPLTTEFTF

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=267, Percent_Identity=28.0898876404494, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI18375503, Length=267, Percent_Identity=28.0898876404494, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI18375501, Length=267, Percent_Identity=28.0898876404494, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1788046, Length=273, Percent_Identity=34.7985347985348, Blast_Score=119, Evalue=2e-28,
Organism=Drosophila melanogaster, GI221330655, Length=279, Percent_Identity=30.4659498207885, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI17136678, Length=279, Percent_Identity=30.4659498207885, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 30888; Mature: 30888

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSVTTWNINSVRLRMPIVERFIDQEAPDVLCLQEIKCQEHQFPYEAFRKLGYEHFAVHG
CCEEEEECCCCEEEECHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHCCCCEEEEEC
QKGYHGVATVGKVPFTEFSRHDWQDNGEARHVGIELTEGPAKGTVIENVYVPAGGDIPDR
CCCCCCHHHCCCCCCHHHHCCCCCCCCCEEEEEEEEECCCCCCCEEEEEEECCCCCCCCC
EQNLKFGQKLDFLERMTRWADKVDRPTLIVGDFNIAPLESDVWNHKQLLKVVSHTPVEVE
HHCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCHH
TLQRFMDAHGWSDIGREHIRAPERYYSWWSYRSPDWRKNDRGRRLDHMWASPELAAQATG
HHHHHHHHCCCHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHH
HRLLEDARSWEKPSDHIPLTTEFTF
HHHHHHHHHCCCCCCCCCEEEECCC
>Mature Secondary Structure
MVSVTTWNINSVRLRMPIVERFIDQEAPDVLCLQEIKCQEHQFPYEAFRKLGYEHFAVHG
CCEEEEECCCCEEEECHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHCCCCEEEEEC
QKGYHGVATVGKVPFTEFSRHDWQDNGEARHVGIELTEGPAKGTVIENVYVPAGGDIPDR
CCCCCCHHHCCCCCCHHHHCCCCCCCCCEEEEEEEEECCCCCCCEEEEEEECCCCCCCCC
EQNLKFGQKLDFLERMTRWADKVDRPTLIVGDFNIAPLESDVWNHKQLLKVVSHTPVEVE
HHCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCHH
TLQRFMDAHGWSDIGREHIRAPERYYSWWSYRSPDWRKNDRGRRLDHMWASPELAAQATG
HHHHHHHHCCCHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHH
HRLLEDARSWEKPSDHIPLTTEFTF
HHHHHHHHHCCCCCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]