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The map label for this gene is nth [H]

Identifier: 85375166

GI number: 85375166

Start: 2354556

End: 2355230

Strand: Reverse

Name: nth [H]

Synonym: ELI_11695

Alternate gene names: 85375166

Gene position: 2355230-2354556 (Counterclockwise)

Preceding gene: 85375167

Following gene: 85375162

Centisome position: 77.16

GC content: 65.48

Gene sequence:

>675_bases
ATGGCCAGGCTCCTCTCCGACAGCGACGTCGAAACCGTATTCGAGCGACTGCGCGAGGCGATGCCGGGGCGGACAAAGAA
TGCGAAAGGCCCCAAGGGCCAGCCCGACGCCTATCGCAGCTGCATTTCCTGCATGCTCTCGGCGCAATCGCTCGACAGCA
ACACCGCCAAGGCGGCCCGCGCGCTGTTTGCGCTCGCGACCAGCCCGGAAGAAATGCTCGCGCTCGACGACGAAGCGATC
GCGCAGGCGATCAAGCCCTGCGGGCTCTACAACATGAAGACGCGAAACATCCGCAAGTTCAACCAGGCGCTGCTGGCCGA
GCATCGCGGGGTGGTCCCCGACACGCGCGAAGGGCTGTTGTCGCTGCCCGGCATCGGCCGCAAATGCGCGGACATCGTCA
TGAGCTTCACCTTCGGCAAGGACGTGATCGCGGTCGACACGCATGTGCATCGCGTCTGCAACCGCATCGGCCTGACCGAC
GCGAAGACCGCCGACAAGACCGCCGAACAGTTGGAGGAGCGCGCGCCCAGATGGGCCCATGCCGACGGGCATTTCTGGCT
CATCCAGTTCGGCAAGAGGGTCTGTACCAGCCGCGCGCCCAAGTGCGAGCGGTGCCCGGTGAGCGACCTCTGCTTATGGT
ACGCCGAGCAGCAGGCGCAGGCCGCTACCAGCTAA

Upstream 100 bases:

>100_bases
CCAGACCCATTGCCGCCCGCGTGCGCCGCACTGCGAGGGTTGCCCGGTACGCGCGGATTGCCGGACCGGAACCGGAGCGC
GCGCCGCTCGCTGAAGCGGT

Downstream 100 bases:

>100_bases
ACCCCGCCTCGATCGCGCGCTGTTCCTTCGCGGTAGTTTCGCGGCCGAGCACTTCATTGCGATGCGGGAAGCGGCCGAAA
CGCTCGATCATCGCGCGATG

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MARLLSDSDVETVFERLREAMPGRTKNAKGPKGQPDAYRSCISCMLSAQSLDSNTAKAARALFALATSPEEMLALDDEAI
AQAIKPCGLYNMKTRNIRKFNQALLAEHRGVVPDTREGLLSLPGIGRKCADIVMSFTFGKDVIAVDTHVHRVCNRIGLTD
AKTADKTAEQLEERAPRWAHADGHFWLIQFGKRVCTSRAPKCERCPVSDLCLWYAEQQAQAATS

Sequences:

>Translated_224_residues
MARLLSDSDVETVFERLREAMPGRTKNAKGPKGQPDAYRSCISCMLSAQSLDSNTAKAARALFALATSPEEMLALDDEAI
AQAIKPCGLYNMKTRNIRKFNQALLAEHRGVVPDTREGLLSLPGIGRKCADIVMSFTFGKDVIAVDTHVHRVCNRIGLTD
AKTADKTAEQLEERAPRWAHADGHFWLIQFGKRVCTSRAPKCERCPVSDLCLWYAEQQAQAATS
>Mature_223_residues
ARLLSDSDVETVFERLREAMPGRTKNAKGPKGQPDAYRSCISCMLSAQSLDSNTAKAARALFALATSPEEMLALDDEAIA
QAIKPCGLYNMKTRNIRKFNQALLAEHRGVVPDTREGLLSLPGIGRKCADIVMSFTFGKDVIAVDTHVHRVCNRIGLTDA
KTADKTAEQLEERAPRWAHADGHFWLIQFGKRVCTSRAPKCERCPVSDLCLWYAEQQAQAATS

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=179, Percent_Identity=34.6368715083799, Blast_Score=104, Evalue=8e-23,
Organism=Escherichia coli, GI1787920, Length=203, Percent_Identity=39.4088669950739, Blast_Score=150, Evalue=8e-38,
Organism=Escherichia coli, GI1789331, Length=133, Percent_Identity=29.3233082706767, Blast_Score=64, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17554540, Length=169, Percent_Identity=33.7278106508876, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324530, Length=195, Percent_Identity=29.7435897435897, Blast_Score=86, Evalue=5e-18,
Organism=Drosophila melanogaster, GI45550361, Length=177, Percent_Identity=27.683615819209, Blast_Score=87, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24710; Mature: 24578

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
4.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARLLSDSDVETVFERLREAMPGRTKNAKGPKGQPDAYRSCISCMLSAQSLDSNTAKAAR
CCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHH
ALFALATSPEEMLALDDEAIAQAIKPCGLYNMKTRNIRKFNQALLAEHRGVVPDTREGLL
HHHHHHCCHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHH
SLPGIGRKCADIVMSFTFGKDVIAVDTHVHRVCNRIGLTDAKTADKTAEQLEERAPRWAH
CCCCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCC
ADGHFWLIQFGKRVCTSRAPKCERCPVSDLCLWYAEQQAQAATS
CCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ARLLSDSDVETVFERLREAMPGRTKNAKGPKGQPDAYRSCISCMLSAQSLDSNTAKAAR
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHH
ALFALATSPEEMLALDDEAIAQAIKPCGLYNMKTRNIRKFNQALLAEHRGVVPDTREGLL
HHHHHHCCHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHH
SLPGIGRKCADIVMSFTFGKDVIAVDTHVHRVCNRIGLTDAKTADKTAEQLEERAPRWAH
CCCCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCC
ADGHFWLIQFGKRVCTSRAPKCERCPVSDLCLWYAEQQAQAATS
CCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12522265 [H]