Click here to switch to the map view.

The map label for this gene is nth [H]

Identifier: 85375167

GI number: 85375167

Start: 2355237

End: 2355914

Strand: Reverse

Name: nth [H]

Synonym: ELI_11700

Alternate gene names: 85375167

Gene position: 2355914-2355237 (Counterclockwise)

Preceding gene: 85375169

Following gene: 85375166

Centisome position: 77.18

GC content: 67.26

Gene sequence:

>678_bases
ATGCTGCGCCGCTTGCAGGCGGCACTGATTGCGCGGTTTGGGCGGATCGTGCGCCCCGCTGACATGCGTCGGCCGCCCGA
GTGGGTGCTGGTGCATGGGGTGATCGGGGCGCAGACCAAGACGGCGGCTTCCAATGCTTCGACCGATGGCCTGCTGGCGG
AATGCGGGTCATGGGAAGCAGTCGCGGCGGCGCCGGTTGAAGAACTGGAAGTGCGGCTGCAGCGGCAGACCTTTCCCTCG
GTCGCGGCGCAAAGGCTGAAGGACTGCCTCAATGCCATCATCGCCGAGCGCGGCGCGGTGGATTTGCGGCACCTGTCCAA
TCTCGCGATCGAGGATGCGATGGAATGGCTCGAGCGCCTGCCCGGGGTCGCGCGCAAGAACAGCGCCGGGGTGATGAATG
CGAGCCTCTTCGAGCGCAAGGCGATGGTCGTCGACGGGCACCACCGCCGCATTATGCAGCGGATGGGTATGGTCCCGCCC
AAGGCCGATACCGCCAGGACCTACGATGCGTTGATGCCGATCGTGCCCGAAGAATGGAGCGCGGCGGACATGGACGAGCA
TCACCTGCTGCTGAAGAAACTCGGCCAGACCCATTGCCGCCCGCGTGCGCCGCACTGCGAGGGTTGCCCGGTACGCGCGG
ATTGCCGGACCGGAACCGGAGCGCGCGCCGCTCGCTGA

Upstream 100 bases:

>100_bases
TATTGTCGACCCTGAGGCCTTGCCTCCCTTCGACAGCCCTCCTGTCATCGGTTACGGGGCTGACATGCAACTCCCCCTCG
GCTCAGACCCACGCACCAAT

Downstream 100 bases:

>100_bases
AGCGGTATGGCCAGGCTCCTCTCCGACAGCGACGTCGAAACCGTATTCGAGCGACTGCGCGAGGCGATGCCGGGGCGGAC
AAAGAATGCGAAAGGCCCCA

Product: endonuclease III family protein

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MLRRLQAALIARFGRIVRPADMRRPPEWVLVHGVIGAQTKTAASNASTDGLLAECGSWEAVAAAPVEELEVRLQRQTFPS
VAAQRLKDCLNAIIAERGAVDLRHLSNLAIEDAMEWLERLPGVARKNSAGVMNASLFERKAMVVDGHHRRIMQRMGMVPP
KADTARTYDALMPIVPEEWSAADMDEHHLLLKKLGQTHCRPRAPHCEGCPVRADCRTGTGARAAR

Sequences:

>Translated_225_residues
MLRRLQAALIARFGRIVRPADMRRPPEWVLVHGVIGAQTKTAASNASTDGLLAECGSWEAVAAAPVEELEVRLQRQTFPS
VAAQRLKDCLNAIIAERGAVDLRHLSNLAIEDAMEWLERLPGVARKNSAGVMNASLFERKAMVVDGHHRRIMQRMGMVPP
KADTARTYDALMPIVPEEWSAADMDEHHLLLKKLGQTHCRPRAPHCEGCPVRADCRTGTGARAAR
>Mature_225_residues
MLRRLQAALIARFGRIVRPADMRRPPEWVLVHGVIGAQTKTAASNASTDGLLAECGSWEAVAAAPVEELEVRLQRQTFPS
VAAQRLKDCLNAIIAERGAVDLRHLSNLAIEDAMEWLERLPGVARKNSAGVMNASLFERKAMVVDGHHRRIMQRMGMVPP
KADTARTYDALMPIVPEEWSAADMDEHHLLLKKLGQTHCRPRAPHCEGCPVRADCRTGTGARAAR

Specific function: Has Both An Apurinic And/Or Apyrimidinic Endonuclease Activity And A DNA N-Glycosylase Activity. Incises Damaged DNA At Cytosines, Thymines And Guanines. Acts On A Damaged Strand, 5' From The Damaged Site. Required For The Repair Of Both Oxidative DNA Da

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24820; Mature: 24820

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRRLQAALIARFGRIVRPADMRRPPEWVLVHGVIGAQTKTAASNASTDGLLAECGSWEA
CHHHHHHHHHHHHHHHCCCHHCCCCCCEEEEECHHCCCHHHHCCCCCCCCHHHHCCCCCH
VAAAPVEELEVRLQRQTFPSVAAQRLKDCLNAIIAERGAVDLRHLSNLAIEDAMEWLERL
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
PGVARKNSAGVMNASLFERKAMVVDGHHRRIMQRMGMVPPKADTARTYDALMPIVPEEWS
CCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCC
AADMDEHHLLLKKLGQTHCRPRAPHCEGCPVRADCRTGTGARAAR
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLRRLQAALIARFGRIVRPADMRRPPEWVLVHGVIGAQTKTAASNASTDGLLAECGSWEA
CHHHHHHHHHHHHHHHCCCHHCCCCCCEEEEECHHCCCHHHHCCCCCCCCHHHHCCCCCH
VAAAPVEELEVRLQRQTFPSVAAQRLKDCLNAIIAERGAVDLRHLSNLAIEDAMEWLERL
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
PGVARKNSAGVMNASLFERKAMVVDGHHRRIMQRMGMVPPKADTARTYDALMPIVPEEWS
CCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCC
AADMDEHHLLLKKLGQTHCRPRAPHCEGCPVRADCRTGTGARAAR
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7704260; 8760912; 9384377 [H]