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The map label for this gene is ispE

Identifier: 85374211

GI number: 85374211

Start: 1435165

End: 1435986

Strand: Reverse

Name: ispE

Synonym: ELI_06920

Alternate gene names: 85374211

Gene position: 1435986-1435165 (Counterclockwise)

Preceding gene: 85374212

Following gene: 85374210

Centisome position: 47.04

GC content: 65.21

Gene sequence:

>822_bases
GTGATTACCGAAACCGCCTACGCAAAGATCAACCTTGCGCTGCACGTCCGCAGCAAGCGCGAGGATGGCTACCACGAGAT
CGAAACGCTGTTCGCCTTCGTCGATGCGGGCGATGTGCTGGTGGCGCGGGCGGCGGAGGCGGATCGGCTGGAGACGGTCG
GCGAATTTGCCGATGTGCTCGACAATCCCTTCGATAATCTCGTCGCGCGCACGATGTCGACGCTTCCCCGTGAGGGCGGG
CTGCATGTCACGCTGGAGAAGAACCTGCCCGTCGCAGCGGGGCTCGGCGGCGGATCGGCCGATGCAGGGGCGATGTTCCG
CATCATCGAGCAGATGCATGGGCTGCCTGATGACTGGGAAGAGAAGGCCATCCGCCTTGGCGCCGATGTGCCAGCCTGCG
TCGCCAGCGAGATGGCGATCGGGCGCGGGACCGGGACCGACCTCGAACCGGTCGAGAACGACATGGCGGGTATGGCGGTG
CTGCTGGTCAACCTGCGTGTGCCGCTGCCGACCGGGCCGGTCTTCAAGGCGTGGGCCGACACCGCAGGCGGTGAGGATCT
TGGGCCACTGCCCACAGGCACCGCGCGCGAAATCGCCCGCAAGGGGCGGAACGACCTGAGACCTCCCGCGGTCGCCATCT
GCCCTCCCATCGCGGACGTCCTTCAGGCGCTGGAGGCAACCGATCCGTGGATGTGCGAAATGTCTGGATCCGGGGCGACC
TGTTTTGCGTTGTATGATGAAACCGAAATGCGCGATAAGGCCGCGCGAGCCATCGCCGAACATCACCCGGGCTGGTGGCA
GATGACAGGAACTCTCCAATGA

Upstream 100 bases:

>100_bases
TCGAGATTTGCGATGCCAGGCGAGTGGAATTGAAGCGGAAAGCTCGCGCGCCCCTCCACCATCCTGCGGATGGTCCCCCT
CCCCAGATCGGGGAAGAAGG

Downstream 100 bases:

>100_bases
TTCTCGACGACAATCCCTATCGCCAGGTCGGCGAGCCGATTCCCGGCAAGATCGTATGCGTGGCCGACCACGCCTCGAAC
TTCGTGCCGGAGGATATCGA

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MITETAYAKINLALHVRSKREDGYHEIETLFAFVDAGDVLVARAAEADRLETVGEFADVLDNPFDNLVARTMSTLPREGG
LHVTLEKNLPVAAGLGGGSADAGAMFRIIEQMHGLPDDWEEKAIRLGADVPACVASEMAIGRGTGTDLEPVENDMAGMAV
LLVNLRVPLPTGPVFKAWADTAGGEDLGPLPTGTAREIARKGRNDLRPPAVAICPPIADVLQALEATDPWMCEMSGSGAT
CFALYDETEMRDKAARAIAEHHPGWWQMTGTLQ

Sequences:

>Translated_273_residues
MITETAYAKINLALHVRSKREDGYHEIETLFAFVDAGDVLVARAAEADRLETVGEFADVLDNPFDNLVARTMSTLPREGG
LHVTLEKNLPVAAGLGGGSADAGAMFRIIEQMHGLPDDWEEKAIRLGADVPACVASEMAIGRGTGTDLEPVENDMAGMAV
LLVNLRVPLPTGPVFKAWADTAGGEDLGPLPTGTAREIARKGRNDLRPPAVAICPPIADVLQALEATDPWMCEMSGSGAT
CFALYDETEMRDKAARAIAEHHPGWWQMTGTLQ
>Mature_273_residues
MITETAYAKINLALHVRSKREDGYHEIETLFAFVDAGDVLVARAAEADRLETVGEFADVLDNPFDNLVARTMSTLPREGG
LHVTLEKNLPVAAGLGGGSADAGAMFRIIEQMHGLPDDWEEKAIRLGADVPACVASEMAIGRGTGTDLEPVENDMAGMAV
LLVNLRVPLPTGPVFKAWADTAGGEDLGPLPTGTAREIARKGRNDLRPPAVAICPPIADVLQALEATDPWMCEMSGSGAT
CFALYDETEMRDKAARAIAEHHPGWWQMTGTLQ

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=277, Percent_Identity=29.9638989169675, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_ERYLH (Q2NA15)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_458273.1
- ProteinModelPortal:   Q2NA15
- SMR:   Q2NA15
- STRING:   Q2NA15
- GeneID:   3870896
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_06920
- NMPDR:   fig|314225.3.peg.1664
- eggNOG:   COG1947
- HOGENOM:   HBG734593
- OMA:   PAVMVNP
- ProtClustDB:   PRK14608
- BioCyc:   ELIT314225:ELI_06920-MONOMER
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 29217; Mature: 29217

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: NA

Important sites: ACT_SITE 9-9 ACT_SITE 129-129

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITETAYAKINLALHVRSKREDGYHEIETLFAFVDAGDVLVARAAEADRLETVGEFADVL
CCCCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHH
DNPFDNLVARTMSTLPREGGLHVTLEKNLPVAAGLGGGSADAGAMFRIIEQMHGLPDDWE
CCHHHHHHHHHHHHCCCCCCEEEEEECCCCEEECCCCCCCCHHHHHHHHHHHCCCCCCHH
EKAIRLGADVPACVASEMAIGRGTGTDLEPVENDMAGMAVLLVNLRVPLPTGPVFKAWAD
HHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHEEEEECCCCCCCHHHHHHC
TAGGEDLGPLPTGTAREIARKGRNDLRPPAVAICPPIADVLQALEATDPWMCEMSGSGAT
CCCCCCCCCCCCCHHHHHHHCCCCCCCCCCEEECCCHHHHHHHHHCCCCEEEEECCCCCE
CFALYDETEMRDKAARAIAEHHPGWWQMTGTLQ
EEEEECCHHHHHHHHHHHHHHCCCEEEEECCCC
>Mature Secondary Structure
MITETAYAKINLALHVRSKREDGYHEIETLFAFVDAGDVLVARAAEADRLETVGEFADVL
CCCCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHH
DNPFDNLVARTMSTLPREGGLHVTLEKNLPVAAGLGGGSADAGAMFRIIEQMHGLPDDWE
CCHHHHHHHHHHHHCCCCCCEEEEEECCCCEEECCCCCCCCHHHHHHHHHHHCCCCCCHH
EKAIRLGADVPACVASEMAIGRGTGTDLEPVENDMAGMAVLLVNLRVPLPTGPVFKAWAD
HHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHEEEEECCCCCCCHHHHHHC
TAGGEDLGPLPTGTAREIARKGRNDLRPPAVAICPPIADVLQALEATDPWMCEMSGSGAT
CCCCCCCCCCCCCHHHHHHHCCCCCCCCCCEEECCCHHHHHHHHHCCCCEEEEECCCCCE
CFALYDETEMRDKAARAIAEHHPGWWQMTGTLQ
EEEEECCHHHHHHHHHHHHHHCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA