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The map label for this gene is mrcA [H]

Identifier: 85374179

GI number: 85374179

Start: 1398030

End: 1400531

Strand: Reverse

Name: mrcA [H]

Synonym: ELI_06760

Alternate gene names: 85374179

Gene position: 1400531-1398030 (Counterclockwise)

Preceding gene: 85374180

Following gene: 85374178

Centisome position: 45.88

GC content: 64.59

Gene sequence:

>2502_bases
ATGGCTGACGGTTCCTCGTTCGAAAACTTTCGCTACATCATCCGCCGCGAAGCGACGGGTGCCTGGGCCTTCCTGCGCCG
CAACTGGCAGGAGAAGCGGCTGTTCCGCTGGGGCGCGACCTTGCTCGGGCTTGCCATTGTCGCCTGGCTGATCGGCTGGG
CCCTGCTTGCGCGCAACCTTCCCGACGCGGAAACCCTGCTGGAATACGAGCCGCCGCTGCCCACGGTCGTGCGCGGGGTC
GATGGCGAGATCGTCCATTCCTATGCCCGCGAGCGGCGCGTTCAGTTGCAATATGCGGACTTCCCCGAACAGCTGATCGA
GGCGTATCTCGCCGCCGAGGACAAGACCTTCTTCAGCCACGGCGGGATCGATGCCGGCGGCCTTGTCGGCGCCGTGGTCG
ATTATGTCAGCAAGATGGGTTCTGGCGAACGTGCCGTCGGCGGGTCGACCATCACGCAGCAGGTGGCGAAGAACATCCTG
CTGTCGAACGAATATTCGATCACCCGCAAGCTCAAGGAAATGATGCTCGCGACCCGCATCGAAGGCGTGCTGAGCAAGCA
GGAAATCCTCGAGCTCTATCTCAACGAGATCCCGCTGGGGCGGCGCTCGTTCGGGGTACAGGCGGCAGCGCGCGCCTATT
TCGACAAGGATGTCGGCGATCTCGAGCTTCACGAATCGGCTTTCCTCGCCGTGCTGCCCAAGGCCCCCGAACGCTATGGC
CGCAAAGGGCAGGAACAGGCCGCGCTGGCCCGGCGCAATTTCGTGATCGACCAGATGGTCGACAATGGCTTCATCGAACA
GAGCGCGGCGACCATCGCCAAACAGCAACCGCTCGGCCTTGTTACCCAGCGCCGCCAACGCTCGGCCGACGCCGGCTATT
TCCTCGAGGAAGTGCGCCGACAGTTGATCGCAGAGTTCGGCGAGACCGTCGATGACGGCGACAACAGCGTCTATGGCGGC
GGGCTCTGGGTGCGTACCTCGCTCGATGTCGAAATGCAGGACGCCGCGCGCGACGCGCTGCGCGAAGGCCTGCTGCGGTA
TCACGGAAGCAAGGGCTGGCGCGGCCCGATCGCGACGATCGACATCAGCGAAGGCAATTGGCAAAGCCAGCTTGCCAGCT
CTTTCCTCGGAATCAATTACGAGAACTGGCGCATCGGTCTTGTGACGGAGCGCAGCGGCAGCTCGGCCACGGTCGGCTTC
GCGGACGGCGAAACCGCGCCGCTGAGCGGTCTGCCCACTGCGCTGAAGGCCGGCGATGTGATCGCCGTTTCGCCGCAAGG
CAATGGTTACCGCGTGCGCACCATTCCCGAAGCATCTGGTGGGGTGGTGGTGCAAGATCCGATGACCGGCCGTGTGCTGG
CCATGCAGGGCGGATTCGACAGCAGGCTGGGGGCGTTCAACCGCGCCACGCAGGCCGAACGGCAGCCGGGGTCGACCATC
AAGCCCTTCGTCTATGCCACGGGGCTGGACAACGGTTTCACCCCCGCTACCGAAGTCGATAATTCCAGCTATTGCTATTA
TCAGGGCTCGCGCCTGGGTGAGAAATGCTTTCGTGGCGGGCGCGGGGGGACCTATCCGCTGCGCTACGGGCTCGAGCAGT
CGCAGAACATCATGACCGTCCAGATCGCAATGGAATCGGGCATGCCCAATGTCATCAAGACGATCGAGAATGTCGGCATC
GGGTCCTACCAGCCTTATCCGGCGTTCGCGCTGGGGGCGGGTGAGACGACGGTTCTCAAGATGGTCAATGCCTATTCGGC
CCTGGCCAATCACGGCCGTCTCAACGATCCGACGCTGATCGACTATGTGCAGGACCGCAGCGGCAAGGTCATCTGGCGCG
CCGACAAGCGATCGTGCGAAGGCTGCAACATGGCCGAATGGGACGGCGGCGCGATGCCGCGGCTGCAGCCCTCGGGCAAG
CAGGCGATGGATGCGCGCACCGCTTTCCAGACCATGCACATGCTCGAAGGCGTGGTGACGCGCGGCACGGGCAAGCGCCT
GGCCGACCTCGAACTGCCGATCTTCGGCAAGACCGGCACCACTACTGGCCCGACCGATGTCTGGTTCGTCGGCGGCACGC
AGGATTATGTCATCGGTGCCTATGTCGGGTTCGACCAGCCGCGCAATCTCGGTGGCTGGGTCGAAGGCGGCAATACCGCC
TCGCCGATCATCAAGAGCGTCATCCAGGCCACCAGGGACCGCTGGGACGACGATCCGGCGATCGCTCCGCCGGGTGTGCG
GATGGTCCGCGTGGACCGGCGCACGGGCAAGCGGGTGTTCGACGGCTGGCCGGGCAATGGCGGCGACATCATCTGGGAAG
CTTTCAAGCCCGATACCGAGCCGCCACGCTCGACTCGCGCCGACGAAATCGCGGCCCAGCGCAAGCAGATCCTCGAACTG
ATCCGCCGCGGGAGCACCGCCGGCTCGCGCAGTGGCGAGCAGGAGGTCACCGGCGAAGCCCCGCCTGCCGATTTCGTGGA
AGACCAAGGCGGCATCTACTAG

Upstream 100 bases:

>100_bases
ATATATTTCGCAAGACAGAGTTAGAGCATGAGAACGGCGCATAATCACGCAATTTCGTATGGCCTGCGTTCAGTTGAGCG
TGTTATGAGCCCCGCCGATC

Downstream 100 bases:

>100_bases
ACAGTGTTCGACCGGCCGCTTGGCGACAGCGCGCGTTTGGGCCATTGGCCCGCGCGTGCACTTTCGCTAAGCCTCGCGTC
CAATCGATCCGAAGGGAATT

Product: membrane carboxypeptidase

Products: NA

Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 833; Mature: 832

Protein sequence:

>833_residues
MADGSSFENFRYIIRREATGAWAFLRRNWQEKRLFRWGATLLGLAIVAWLIGWALLARNLPDAETLLEYEPPLPTVVRGV
DGEIVHSYARERRVQLQYADFPEQLIEAYLAAEDKTFFSHGGIDAGGLVGAVVDYVSKMGSGERAVGGSTITQQVAKNIL
LSNEYSITRKLKEMMLATRIEGVLSKQEILELYLNEIPLGRRSFGVQAAARAYFDKDVGDLELHESAFLAVLPKAPERYG
RKGQEQAALARRNFVIDQMVDNGFIEQSAATIAKQQPLGLVTQRRQRSADAGYFLEEVRRQLIAEFGETVDDGDNSVYGG
GLWVRTSLDVEMQDAARDALREGLLRYHGSKGWRGPIATIDISEGNWQSQLASSFLGINYENWRIGLVTERSGSSATVGF
ADGETAPLSGLPTALKAGDVIAVSPQGNGYRVRTIPEASGGVVVQDPMTGRVLAMQGGFDSRLGAFNRATQAERQPGSTI
KPFVYATGLDNGFTPATEVDNSSYCYYQGSRLGEKCFRGGRGGTYPLRYGLEQSQNIMTVQIAMESGMPNVIKTIENVGI
GSYQPYPAFALGAGETTVLKMVNAYSALANHGRLNDPTLIDYVQDRSGKVIWRADKRSCEGCNMAEWDGGAMPRLQPSGK
QAMDARTAFQTMHMLEGVVTRGTGKRLADLELPIFGKTGTTTGPTDVWFVGGTQDYVIGAYVGFDQPRNLGGWVEGGNTA
SPIIKSVIQATRDRWDDDPAIAPPGVRMVRVDRRTGKRVFDGWPGNGGDIIWEAFKPDTEPPRSTRADEIAAQRKQILEL
IRRGSTAGSRSGEQEVTGEAPPADFVEDQGGIY

Sequences:

>Translated_833_residues
MADGSSFENFRYIIRREATGAWAFLRRNWQEKRLFRWGATLLGLAIVAWLIGWALLARNLPDAETLLEYEPPLPTVVRGV
DGEIVHSYARERRVQLQYADFPEQLIEAYLAAEDKTFFSHGGIDAGGLVGAVVDYVSKMGSGERAVGGSTITQQVAKNIL
LSNEYSITRKLKEMMLATRIEGVLSKQEILELYLNEIPLGRRSFGVQAAARAYFDKDVGDLELHESAFLAVLPKAPERYG
RKGQEQAALARRNFVIDQMVDNGFIEQSAATIAKQQPLGLVTQRRQRSADAGYFLEEVRRQLIAEFGETVDDGDNSVYGG
GLWVRTSLDVEMQDAARDALREGLLRYHGSKGWRGPIATIDISEGNWQSQLASSFLGINYENWRIGLVTERSGSSATVGF
ADGETAPLSGLPTALKAGDVIAVSPQGNGYRVRTIPEASGGVVVQDPMTGRVLAMQGGFDSRLGAFNRATQAERQPGSTI
KPFVYATGLDNGFTPATEVDNSSYCYYQGSRLGEKCFRGGRGGTYPLRYGLEQSQNIMTVQIAMESGMPNVIKTIENVGI
GSYQPYPAFALGAGETTVLKMVNAYSALANHGRLNDPTLIDYVQDRSGKVIWRADKRSCEGCNMAEWDGGAMPRLQPSGK
QAMDARTAFQTMHMLEGVVTRGTGKRLADLELPIFGKTGTTTGPTDVWFVGGTQDYVIGAYVGFDQPRNLGGWVEGGNTA
SPIIKSVIQATRDRWDDDPAIAPPGVRMVRVDRRTGKRVFDGWPGNGGDIIWEAFKPDTEPPRSTRADEIAAQRKQILEL
IRRGSTAGSRSGEQEVTGEAPPADFVEDQGGIY
>Mature_832_residues
ADGSSFENFRYIIRREATGAWAFLRRNWQEKRLFRWGATLLGLAIVAWLIGWALLARNLPDAETLLEYEPPLPTVVRGVD
GEIVHSYARERRVQLQYADFPEQLIEAYLAAEDKTFFSHGGIDAGGLVGAVVDYVSKMGSGERAVGGSTITQQVAKNILL
SNEYSITRKLKEMMLATRIEGVLSKQEILELYLNEIPLGRRSFGVQAAARAYFDKDVGDLELHESAFLAVLPKAPERYGR
KGQEQAALARRNFVIDQMVDNGFIEQSAATIAKQQPLGLVTQRRQRSADAGYFLEEVRRQLIAEFGETVDDGDNSVYGGG
LWVRTSLDVEMQDAARDALREGLLRYHGSKGWRGPIATIDISEGNWQSQLASSFLGINYENWRIGLVTERSGSSATVGFA
DGETAPLSGLPTALKAGDVIAVSPQGNGYRVRTIPEASGGVVVQDPMTGRVLAMQGGFDSRLGAFNRATQAERQPGSTIK
PFVYATGLDNGFTPATEVDNSSYCYYQGSRLGEKCFRGGRGGTYPLRYGLEQSQNIMTVQIAMESGMPNVIKTIENVGIG
SYQPYPAFALGAGETTVLKMVNAYSALANHGRLNDPTLIDYVQDRSGKVIWRADKRSCEGCNMAEWDGGAMPRLQPSGKQ
AMDARTAFQTMHMLEGVVTRGTGKRLADLELPIFGKTGTTTGPTDVWFVGGTQDYVIGAYVGFDQPRNLGGWVEGGNTAS
PIIKSVIQATRDRWDDDPAIAPPGVRMVRVDRRTGKRVFDGWPGNGGDIIWEAFKPDTEPPRSTRADEIAAQRKQILELI
RRGSTAGSRSGEQEVTGEAPPADFVEDQGGIY

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG5009

COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=839, Percent_Identity=30.9892729439809, Blast_Score=333, Evalue=2e-92,
Organism=Escherichia coli, GI1786343, Length=283, Percent_Identity=31.4487632508834, Blast_Score=109, Evalue=9e-25,
Organism=Escherichia coli, GI1788867, Length=192, Percent_Identity=32.2916666666667, Blast_Score=97, Evalue=5e-21,
Organism=Escherichia coli, GI1789601, Length=163, Percent_Identity=36.1963190184049, Blast_Score=83, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.-

Molecular weight: Translated: 91130; Mature: 90999

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADGSSFENFRYIIRREATGAWAFLRRNWQEKRLFRWGATLLGLAIVAWLIGWALLARNL
CCCCCCCHHHHHHHEECCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PDAETLLEYEPPLPTVVRGVDGEIVHSYARERRVQLQYADFPEQLIEAYLAAEDKTFFSH
CCHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHEEEEEECCCHHHHHHHHHHCCCCCHHHC
GGIDAGGLVGAVVDYVSKMGSGERAVGGSTITQQVAKNILLSNEYSITRKLKEMMLATRI
CCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
EGVLSKQEILELYLNEIPLGRRSFGVQAAARAYFDKDVGDLELHESAFLAVLPKAPERYG
HHHHHHHHHHHHHHHHCCCCCHHHCHHHHHHHHHCCCCCCCEECCCEEEEECCCCHHHHC
RKGQEQAALARRNFVIDQMVDNGFIEQSAATIAKQQPLGLVTQRRQRSADAGYFLEEVRR
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHH
QLIAEFGETVDDGDNSVYGGGLWVRTSLDVEMQDAARDALREGLLRYHGSKGWRGPIATI
HHHHHHCCCCCCCCCCEECCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
DISEGNWQSQLASSFLGINYENWRIGLVTERSGSSATVGFADGETAPLSGLPTALKAGDV
ECCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCEEEEECCCCCCCCCCCCHHHCCCCE
IAVSPQGNGYRVRTIPEASGGVVVQDPMTGRVLAMQGGFDSRLGAFNRATQAERQPGSTI
EEECCCCCCEEEEECCCCCCCEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCC
KPFVYATGLDNGFTPATEVDNSSYCYYQGSRLGEKCFRGGRGGTYPLRYGLEQSQNIMTV
CCEEEEECCCCCCCCCCCCCCCEEEEEECCHHHHHHHCCCCCCCCCEEECCCCCCCEEEE
QIAMESGMPNVIKTIENVGIGSYQPYPAFALGAGETTVLKMVNAYSALANHGRLNDPTLI
EEEECCCCCHHHHHHHHCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCCCCHHH
DYVQDRSGKVIWRADKRSCEGCNMAEWDGGAMPRLQPSGKQAMDARTAFQTMHMLEGVVT
HHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
RGTGKRLADLELPIFGKTGTTTGPTDVWFVGGTQDYVIGAYVGFDQPRNLGGWVEGGNTA
CCCCCEEEEEECEEECCCCCCCCCCEEEEECCCCCEEEEEEECCCCCCCCCCEECCCCCH
SPIIKSVIQATRDRWDDDPAIAPPGVRMVRVDRRTGKRVFDGWPGNGGDIIWEAFKPDTE
HHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCEEECCCCCCCCCEEEEECCCCCC
PPRSTRADEIAAQRKQILELIRRGSTAGSRSGEQEVTGEAPPADFVEDQGGIY
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCC
>Mature Secondary Structure 
ADGSSFENFRYIIRREATGAWAFLRRNWQEKRLFRWGATLLGLAIVAWLIGWALLARNL
CCCCCCHHHHHHHEECCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PDAETLLEYEPPLPTVVRGVDGEIVHSYARERRVQLQYADFPEQLIEAYLAAEDKTFFSH
CCHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHEEEEEECCCHHHHHHHHHHCCCCCHHHC
GGIDAGGLVGAVVDYVSKMGSGERAVGGSTITQQVAKNILLSNEYSITRKLKEMMLATRI
CCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
EGVLSKQEILELYLNEIPLGRRSFGVQAAARAYFDKDVGDLELHESAFLAVLPKAPERYG
HHHHHHHHHHHHHHHHCCCCCHHHCHHHHHHHHHCCCCCCCEECCCEEEEECCCCHHHHC
RKGQEQAALARRNFVIDQMVDNGFIEQSAATIAKQQPLGLVTQRRQRSADAGYFLEEVRR
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHH
QLIAEFGETVDDGDNSVYGGGLWVRTSLDVEMQDAARDALREGLLRYHGSKGWRGPIATI
HHHHHHCCCCCCCCCCEECCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
DISEGNWQSQLASSFLGINYENWRIGLVTERSGSSATVGFADGETAPLSGLPTALKAGDV
ECCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCEEEEECCCCCCCCCCCCHHHCCCCE
IAVSPQGNGYRVRTIPEASGGVVVQDPMTGRVLAMQGGFDSRLGAFNRATQAERQPGSTI
EEECCCCCCEEEEECCCCCCCEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCC
KPFVYATGLDNGFTPATEVDNSSYCYYQGSRLGEKCFRGGRGGTYPLRYGLEQSQNIMTV
CCEEEEECCCCCCCCCCCCCCCEEEEEECCHHHHHHHCCCCCCCCCEEECCCCCCCEEEE
QIAMESGMPNVIKTIENVGIGSYQPYPAFALGAGETTVLKMVNAYSALANHGRLNDPTLI
EEEECCCCCHHHHHHHHCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCCCCHHH
DYVQDRSGKVIWRADKRSCEGCNMAEWDGGAMPRLQPSGKQAMDARTAFQTMHMLEGVVT
HHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
RGTGKRLADLELPIFGKTGTTTGPTDVWFVGGTQDYVIGAYVGFDQPRNLGGWVEGGNTA
CCCCCEEEEEECEEECCCCCCCCCCEEEEECCCCCEEEEEEECCCCCCCCCCEECCCCCH
SPIIKSVIQATRDRWDDDPAIAPPGVRMVRVDRRTGKRVFDGWPGNGGDIIWEAFKPDTE
HHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCEEECCCCCCCCCEEEEECCCCCC
PPRSTRADEIAAQRKQILELIRRGSTAGSRSGEQEVTGEAPPADFVEDQGGIY
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]