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The map label for this gene is amiC [H]

Identifier: 85374180

GI number: 85374180

Start: 1400608

End: 1401471

Strand: Reverse

Name: amiC [H]

Synonym: ELI_06765

Alternate gene names: 85374180

Gene position: 1401471-1400608 (Counterclockwise)

Preceding gene: 85374181

Following gene: 85374179

Centisome position: 45.91

GC content: 65.97

Gene sequence:

>864_bases
ATGTCGCTGCGCATCCAACTCTGGCTGATATTCCTGTTGCCCGTCCTGCTGGTCGGCGGCCTGGTCGTGTTCGGCCTGAC
GATCCCCGTCCCGCATCTGGGGCGCGATTATGTGCTCGACCTGTTGCTGCCCGATGCCGACGATCCGGTTGACCTGCCGA
CGGTCTATGGCCCGCCCGACCGGTCGCGCCCGCTGGTGGTGATCGACGCAGGGCACGGCGGCAAGGATCCGGGCGCGAGC
GGGGCGGGCTTGCGCGAAAAGACCGTCGTGCTAGGGCTTGCGCAGGCGCTCAAGGACGAATTGCTGGAGCAGGGCGGCAT
TCGTGTCGCGCTGACACGGGAAGACGATACTTTCCTGGTGCTCGACGAGCGACCGGAGATTGCGCGGCGGCTGGATGCCG
ATCTCTTTATCTCGATCCATGCCGATTCTGCTGGAGAGGTGAGCGGTGTCAGCGGCGCAAGCATCTACACTCTGTCGAGC
GAGGCCTCGAGCGAAGCGGCCGCGCGGTTTGCCGAGCGGGAGAACAACGCCGACCGACTGAACGGCGTCGAGGTCGACGG
GCAGAGCGACGTCGTCAGCAACATCCTCGTCGAGCTTTCGCAGCGGCGGGTGCAGGAAAATTCGGCCGAGTTCGCGGGCC
TCGTCGTGCGCGAAGGGCGGGGCAATCTGCGCTTCCACCCGCAGGCGCGCCGGTCAGCTGCCCTTGCCGTGCTGCGCGCA
CCCGATGTGCCGGCCGTACTGTACGAATCCGGCTATATCACCAACCCGGCCGATGCCGCGCGACTCTCTTCGCCGGAGGG
GCGCAAAGCCTTCGCCGAGACCATGGCGCGGGCGATCCGCATATATTTCGCAAGACAGAGTTAG

Upstream 100 bases:

>100_bases
GATGCGTGTCTCCGGTCGAAGCGGGTGCTAGCATCGCGGGAGAAGTCCGGCAACTATATTGCGCACTTAGCCCCGGCCGC
CCTAACAGGGTGCTAAGAAC

Downstream 100 bases:

>100_bases
AGCATGAGAACGGCGCATAATCACGCAATTTCGTATGGCCTGCGTTCAGTTGAGCGTGTTATGAGCCCCGCCGATCATGG
CTGACGGTTCCTCGTTCGAA

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MSLRIQLWLIFLLPVLLVGGLVVFGLTIPVPHLGRDYVLDLLLPDADDPVDLPTVYGPPDRSRPLVVIDAGHGGKDPGAS
GAGLREKTVVLGLAQALKDELLEQGGIRVALTREDDTFLVLDERPEIARRLDADLFISIHADSAGEVSGVSGASIYTLSS
EASSEAAARFAERENNADRLNGVEVDGQSDVVSNILVELSQRRVQENSAEFAGLVVREGRGNLRFHPQARRSAALAVLRA
PDVPAVLYESGYITNPADAARLSSPEGRKAFAETMARAIRIYFARQS

Sequences:

>Translated_287_residues
MSLRIQLWLIFLLPVLLVGGLVVFGLTIPVPHLGRDYVLDLLLPDADDPVDLPTVYGPPDRSRPLVVIDAGHGGKDPGAS
GAGLREKTVVLGLAQALKDELLEQGGIRVALTREDDTFLVLDERPEIARRLDADLFISIHADSAGEVSGVSGASIYTLSS
EASSEAAARFAERENNADRLNGVEVDGQSDVVSNILVELSQRRVQENSAEFAGLVVREGRGNLRFHPQARRSAALAVLRA
PDVPAVLYESGYITNPADAARLSSPEGRKAFAETMARAIRIYFARQS
>Mature_286_residues
SLRIQLWLIFLLPVLLVGGLVVFGLTIPVPHLGRDYVLDLLLPDADDPVDLPTVYGPPDRSRPLVVIDAGHGGKDPGASG
AGLREKTVVLGLAQALKDELLEQGGIRVALTREDDTFLVLDERPEIARRLDADLFISIHADSAGEVSGVSGASIYTLSSE
ASSEAAARFAERENNADRLNGVEVDGQSDVVSNILVELSQRRVQENSAEFAGLVVREGRGNLRFHPQARRSAALAVLRAP
DVPAVLYESGYITNPADAARLSSPEGRKAFAETMARAIRIYFARQS

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=223, Percent_Identity=36.7713004484305, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI87082163, Length=232, Percent_Identity=34.9137931034483, Blast_Score=130, Evalue=9e-32,
Organism=Escherichia coli, GI1790611, Length=225, Percent_Identity=34.6666666666667, Blast_Score=123, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 30898; Mature: 30767

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLRIQLWLIFLLPVLLVGGLVVFGLTIPVPHLGRDYVLDLLLPDADDPVDLPTVYGPPD
CCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEEEECCCCCCCCCCCCCCCCCC
RSRPLVVIDAGHGGKDPGASGAGLREKTVVLGLAQALKDELLEQGGIRVALTREDDTFLV
CCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEE
LDERPEIARRLDADLFISIHADSAGEVSGVSGASIYTLSSEASSEAAARFAERENNADRL
EECCHHHHHHCCCEEEEEEECCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCHHC
NGVEVDGQSDVVSNILVELSQRRVQENSAEFAGLVVREGRGNLRFHPQARRSAALAVLRA
CCEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEECCHHHHHHEEEEEEC
PDVPAVLYESGYITNPADAARLSSPEGRKAFAETMARAIRIYFARQS
CCCCEEEECCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHEEEECC
>Mature Secondary Structure 
SLRIQLWLIFLLPVLLVGGLVVFGLTIPVPHLGRDYVLDLLLPDADDPVDLPTVYGPPD
CEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEEEECCCCCCCCCCCCCCCCCC
RSRPLVVIDAGHGGKDPGASGAGLREKTVVLGLAQALKDELLEQGGIRVALTREDDTFLV
CCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEE
LDERPEIARRLDADLFISIHADSAGEVSGVSGASIYTLSSEASSEAAARFAERENNADRL
EECCHHHHHHCCCEEEEEEECCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCHHC
NGVEVDGQSDVVSNILVELSQRRVQENSAEFAGLVVREGRGNLRFHPQARRSAALAVLRA
CCEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEECCHHHHHHEEEEEEC
PDVPAVLYESGYITNPADAARLSSPEGRKAFAETMARAIRIYFARQS
CCCCEEEECCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]