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The map label for this gene is prfB [H]

Identifier: 85374178

GI number: 85374178

Start: 1396799

End: 1397926

Strand: Reverse

Name: prfB [H]

Synonym: ELI_06755

Alternate gene names: 85374178

Gene position: 1397926-1396799 (Counterclockwise)

Preceding gene: 85374179

Following gene: 85374177

Centisome position: 45.8

GC content: 63.12

Gene sequence:

>1128_bases
ATGCGTGCCGAAGGGCAGGCCAATATCGACCGTATCGAAGCCGCGCTCGCTCTGGTGCGCCAGTCTCTCGACTGGGAGCG
TGCATTGCGCCGCCTCGACGAGCTCAATGCACGGGTGCAGGACCCGGATCTCTGGAACGATCCCAAGGAAGCGCAGGCGA
TCAGTCGCGAGCAGAAGACTCTCGAAACGGCGGTCAAGACCGTCCGGGAGATCGGCTCGGAAATGGCGGATGCGATCGAG
TTCGTCGAGCTGGGCGAAGCCGAAGGCGACGAAGATGTGGTGAAGGAGGGCCTCGCGAGCCTGCAGAAGCTCGCCGACCG
TGCGGATGCAGACAAGGTCCAGGCGCTGCTCTCGGGCGAAGCCGACGGCAACGACACCTATATCGAAATCCACGCCGGTG
CCGGTGGTACGGAAAGCCAGGACTGGGCCGAAATGCTGCTGCGCATGTATGCGCGCTGGGCCGAGCGGCGCGGCTTCAAG
GTCGAGACGGTTGAATATCAGGCGGGCGACCAAGCCGGTATCAAGTCGGCGACGCTGCTGGTGAAGGGCGAGAATGCCTA
TGGCTACGCCAAGACCGAGAGCGGCGTGCACCGGCTCGTTCGCATCAGTCCATATGACAGCTCGGCACGGCGCCATACGT
CGTTCTCGAGCGTGTGGGTCTATCCGGTGATCGACGACAATATCGAGATCGAGATCAACGACAGCGATCTCAAGATCGAT
ACCTACCGCGCTTCGGGCGCTGGTGGCCAGCACGTCAACACAACGGATTCCGCTGTGCGCATCACCCACCAGCCGACCGG
GATCGTGGTCGCCAGCCAGAACGACCGCAGCCAGCACAAGAACCGCGCGACGGCGATGAATATGCTCAAGGCGCGGCTGT
TCGAACGCGAGATGGCGGAGCGTGAAGCGGCGGCGAGCGGCGAGTATCAGGAAAAGACCGAGATCGGCTGGGGTCACCAG
ATCCGCAGCTATGTCTTGCAGCCCTACCAGATGGTCAAGGATCTGCGCACCGGCGTCACCTCCAGCTCCCCCGATGATGT
GCTCGACGGCGCGCTCGACCCCTACATTTCCGCCGCCCTCGCACAGCGCGTGACGGGCGAGACAGTGGAAGTGGAAGACG
TCGAATGA

Upstream 100 bases:

>100_bases
GTGTTCGACCGGCCGCTTGGCGACAGCGCGCGTTTGGGCCATTGGCCCGCGCGTGCACTTTCGCTAAGCCTCGCGTCCAA
TCGATCCGAAGGGAATTTCC

Downstream 100 bases:

>100_bases
TCCGCTGCGCTGTCTTGTGCCTGCCCGTGCTGGCCATGCTGCCCGCATGCGAGCGGGTGCCCGGCAGCGACACCAGCGAC
CGGCTGGCCAGCGCCATTCA

Product: protein chain release factor B

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MRAEGQANIDRIEAALALVRQSLDWERALRRLDELNARVQDPDLWNDPKEAQAISREQKTLETAVKTVREIGSEMADAIE
FVELGEAEGDEDVVKEGLASLQKLADRADADKVQALLSGEADGNDTYIEIHAGAGGTESQDWAEMLLRMYARWAERRGFK
VETVEYQAGDQAGIKSATLLVKGENAYGYAKTESGVHRLVRISPYDSSARRHTSFSSVWVYPVIDDNIEIEINDSDLKID
TYRASGAGGQHVNTTDSAVRITHQPTGIVVASQNDRSQHKNRATAMNMLKARLFEREMAEREAAASGEYQEKTEIGWGHQ
IRSYVLQPYQMVKDLRTGVTSSSPDDVLDGALDPYISAALAQRVTGETVEVEDVE

Sequences:

>Translated_375_residues
MRAEGQANIDRIEAALALVRQSLDWERALRRLDELNARVQDPDLWNDPKEAQAISREQKTLETAVKTVREIGSEMADAIE
FVELGEAEGDEDVVKEGLASLQKLADRADADKVQALLSGEADGNDTYIEIHAGAGGTESQDWAEMLLRMYARWAERRGFK
VETVEYQAGDQAGIKSATLLVKGENAYGYAKTESGVHRLVRISPYDSSARRHTSFSSVWVYPVIDDNIEIEINDSDLKID
TYRASGAGGQHVNTTDSAVRITHQPTGIVVASQNDRSQHKNRATAMNMLKARLFEREMAEREAAASGEYQEKTEIGWGHQ
IRSYVLQPYQMVKDLRTGVTSSSPDDVLDGALDPYISAALAQRVTGETVEVEDVE
>Mature_375_residues
MRAEGQANIDRIEAALALVRQSLDWERALRRLDELNARVQDPDLWNDPKEAQAISREQKTLETAVKTVREIGSEMADAIE
FVELGEAEGDEDVVKEGLASLQKLADRADADKVQALLSGEADGNDTYIEIHAGAGGTESQDWAEMLLRMYARWAERRGFK
VETVEYQAGDQAGIKSATLLVKGENAYGYAKTESGVHRLVRISPYDSSARRHTSFSSVWVYPVIDDNIEIEINDSDLKID
TYRASGAGGQHVNTTDSAVRITHQPTGIVVASQNDRSQHKNRATAMNMLKARLFEREMAEREAAASGEYQEKTEIGWGHQ
IRSYVLQPYQMVKDLRTGVTSSSPDDVLDGALDPYISAALAQRVTGETVEVEDVE

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=237, Percent_Identity=39.662447257384, Blast_Score=158, Evalue=7e-39,
Organism=Homo sapiens, GI34577120, Length=220, Percent_Identity=37.2727272727273, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI166795305, Length=149, Percent_Identity=44.2953020134228, Blast_Score=125, Evalue=6e-29,
Organism=Escherichia coli, GI2367172, Length=343, Percent_Identity=51.8950437317784, Blast_Score=366, Evalue=1e-102,
Organism=Escherichia coli, GI1787462, Length=238, Percent_Identity=43.6974789915966, Blast_Score=187, Evalue=9e-49,
Organism=Caenorhabditis elegans, GI17542784, Length=243, Percent_Identity=33.3333333333333, Blast_Score=105, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6321295, Length=243, Percent_Identity=37.4485596707819, Blast_Score=134, Evalue=2e-32,
Organism=Drosophila melanogaster, GI19921226, Length=231, Percent_Identity=40.2597402597403, Blast_Score=161, Evalue=9e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 41546; Mature: 41546

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAEGQANIDRIEAALALVRQSLDWERALRRLDELNARVQDPDLWNDPKEAQAISREQKT
CCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
LETAVKTVREIGSEMADAIEFVELGEAEGDEDVVKEGLASLQKLADRADADKVQALLSGE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCC
ADGNDTYIEIHAGAGGTESQDWAEMLLRMYARWAERRGFKVETVEYQAGDQAGIKSATLL
CCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEE
VKGENAYGYAKTESGVHRLVRISPYDSSARRHTSFSSVWVYPVIDDNIEIEINDSDLKID
EECCCCCCCCCCHHHHEEEEEECCCCCCHHHCCCCCCEEEEEEECCCEEEEECCCCEEEE
TYRASGAGGQHVNTTDSAVRITHQPTGIVVASQNDRSQHKNRATAMNMLKARLFEREMAE
EEECCCCCCCCCCCCCCEEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
REAAASGEYQEKTEIGWGHQIRSYVLQPYQMVKDLRTGVTSSSPDDVLDGALDPYISAAL
HHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
AQRVTGETVEVEDVE
HHHHCCCEEEEECCC
>Mature Secondary Structure
MRAEGQANIDRIEAALALVRQSLDWERALRRLDELNARVQDPDLWNDPKEAQAISREQKT
CCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
LETAVKTVREIGSEMADAIEFVELGEAEGDEDVVKEGLASLQKLADRADADKVQALLSGE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCC
ADGNDTYIEIHAGAGGTESQDWAEMLLRMYARWAERRGFKVETVEYQAGDQAGIKSATLL
CCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEE
VKGENAYGYAKTESGVHRLVRISPYDSSARRHTSFSSVWVYPVIDDNIEIEINDSDLKID
EECCCCCCCCCCHHHHEEEEEECCCCCCHHHCCCCCCEEEEEEECCCEEEEECCCCEEEE
TYRASGAGGQHVNTTDSAVRITHQPTGIVVASQNDRSQHKNRATAMNMLKARLFEREMAE
EEECCCCCCCCCCCCCCEEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
REAAASGEYQEKTEIGWGHQIRSYVLQPYQMVKDLRTGVTSSSPDDVLDGALDPYISAAL
HHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
AQRVTGETVEVEDVE
HHHHCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA