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The map label for this gene is birA [H]

Identifier: 85374146

GI number: 85374146

Start: 1369308

End: 1370018

Strand: Reverse

Name: birA [H]

Synonym: ELI_06595

Alternate gene names: 85374146

Gene position: 1370018-1369308 (Counterclockwise)

Preceding gene: 85374147

Following gene: 85374145

Centisome position: 44.88

GC content: 65.68

Gene sequence:

>711_bases
TTGATTCAGACCGTCGCCGAAACCGGCTCGACTAACGCCGACCTGATTTCGCGCATCTGCGGTGGTGATCATGTGCCGGA
AGGGCACTGGCTGGTCGCAGACCGCCAGACAGCCGGCCGAGGACGGCAGGGGCGGACCTGGTTCGATGGTTCGGGCAATT
TCATGGGCTCGACGGTCGTGCATCGGGCGCCGCAGGACCCGCTCGCGCATTCGCTTTCGCTGGTTGCAGGGTTGGCGCTT
TACGAGGCGCTCGCATCGCATGTGTCGGACCCGTCGGGACTGATGCTCAAATGGCCCAACGACGTGCTCCTGCGCGGAGC
CAAGCTGTGCGGTGTTCTTCTGGAGCGCGCAGGCGACGCTGTCGTGATCGGCATCGGGGTTAACCTCGCCGCTGCGCCCG
ACTTACCCGATCGCGAAACTATTGCGCTTTCGCAACTCGGTCCGGCTCCCGACCGCGATCTTTTTGCTGCAGCGCTGGCC
GGAGCGCTGGATCGTGAACTCGAGCGCTGGCGCGCTTATGGTCTCGATCCGCTGATCCGCCGCTGGCTGGTTGGCGGGAC
GCCGGAGGGCACTGCGCTTTCGGTTCATGAACCCGACGGCAGCAGGCTCAGCGGCCGCTTTGCCGGGCTCGACGCCGAGG
GCACGCTGCAACTGCGCTTGGACGACGGGACCATCCGTGCCATTCACGCCGGCGATGTGATGCTCGCCTGA

Upstream 100 bases:

>100_bases
TGATATTGGCGGCGGTGGTCGTTTCGCCACTCGGTTACCTGCTGACGGGCTGGCTCGGCGGGCTGACCGATAGCGCCGCG
TCGGCGCTGTTCATCGCCTC

Downstream 100 bases:

>100_bases
AGGAGCCGAGAGAGACATGCTGCTAGCCGCCGATGTCGGGAACACCAATGTCGTCTTCGCGCTGTTCGAAGGCCGCGACA
TCCGCGCCCGCTGGCGTATC

Product: biotin protein ligase

Products: NA

Alternate protein names: Biotin--protein ligase [H]

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MIQTVAETGSTNADLISRICGGDHVPEGHWLVADRQTAGRGRQGRTWFDGSGNFMGSTVVHRAPQDPLAHSLSLVAGLAL
YEALASHVSDPSGLMLKWPNDVLLRGAKLCGVLLERAGDAVVIGIGVNLAAAPDLPDRETIALSQLGPAPDRDLFAAALA
GALDRELERWRAYGLDPLIRRWLVGGTPEGTALSVHEPDGSRLSGRFAGLDAEGTLQLRLDDGTIRAIHAGDVMLA

Sequences:

>Translated_236_residues
MIQTVAETGSTNADLISRICGGDHVPEGHWLVADRQTAGRGRQGRTWFDGSGNFMGSTVVHRAPQDPLAHSLSLVAGLAL
YEALASHVSDPSGLMLKWPNDVLLRGAKLCGVLLERAGDAVVIGIGVNLAAAPDLPDRETIALSQLGPAPDRDLFAAALA
GALDRELERWRAYGLDPLIRRWLVGGTPEGTALSVHEPDGSRLSGRFAGLDAEGTLQLRLDDGTIRAIHAGDVMLA
>Mature_236_residues
MIQTVAETGSTNADLISRICGGDHVPEGHWLVADRQTAGRGRQGRTWFDGSGNFMGSTVVHRAPQDPLAHSLSLVAGLAL
YEALASHVSDPSGLMLKWPNDVLLRGAKLCGVLLERAGDAVVIGIGVNLAAAPDLPDRETIALSQLGPAPDRDLFAAALA
GALDRELERWRAYGLDPLIRRWLVGGTPEGTALSVHEPDGSRLSGRFAGLDAEGTLQLRLDDGTIRAIHAGDVMLA

Specific function: Activates biotin to form biotinyl-5'-adenylate and transfers the biotin moiety to biotin-accepting proteins [H]

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=237, Percent_Identity=33.7552742616034, Blast_Score=94, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004408
- InterPro:   IPR003142
- InterPro:   IPR004143 [H]

Pfam domain/function: PF02237 BPL_C; PF03099 BPL_LipA_LipB [H]

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 24919; Mature: 24919

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQTVAETGSTNADLISRICGGDHVPEGHWLVADRQTAGRGRQGRTWFDGSGNFMGSTVV
CCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCEEECCCCCCCCCHHH
HRAPQDPLAHSLSLVAGLALYEALASHVSDPSGLMLKWPNDVLLRGAKLCGVLLERAGDA
HCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHCCCCE
VVIGIGVNLAAAPDLPDRETIALSQLGPAPDRDLFAAALAGALDRELERWRAYGLDPLIR
EEEEECCCEEECCCCCCCCEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
RWLVGGTPEGTALSVHEPDGSRLSGRFAGLDAEGTLQLRLDDGTIRAIHAGDVMLA
HHHCCCCCCCCEEEEECCCCCCCCCEEECCCCCCEEEEEECCCEEEEEECCCEEEC
>Mature Secondary Structure
MIQTVAETGSTNADLISRICGGDHVPEGHWLVADRQTAGRGRQGRTWFDGSGNFMGSTVV
CCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCEEECCCCCCCCCHHH
HRAPQDPLAHSLSLVAGLALYEALASHVSDPSGLMLKWPNDVLLRGAKLCGVLLERAGDA
HCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHCCCCE
VVIGIGVNLAAAPDLPDRETIALSQLGPAPDRDLFAAALAGALDRELERWRAYGLDPLIR
EEEEECCCEEECCCCCCCCEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
RWLVGGTPEGTALSVHEPDGSRLSGRFAGLDAEGTLQLRLDDGTIRAIHAGDVMLA
HHHCCCCCCCCEEEEECCCCCCCCCEEECCCCCCEEEEEECCCEEEEEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8422400 [H]