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The map label for this gene is nuoN

Identifier: 85374147

GI number: 85374147

Start: 1370015

End: 1371478

Strand: Reverse

Name: nuoN

Synonym: ELI_06600

Alternate gene names: 85374147

Gene position: 1371478-1370015 (Counterclockwise)

Preceding gene: 85374148

Following gene: 85374146

Centisome position: 44.93

GC content: 64.21

Gene sequence:

>1464_bases
ATGGAACTCAATGCATCCCTCACGCTGATCCTCCCCGAGATCGTCATGGCGATCTCGGCCATGGCGCTGATCCTGATCAC
TGCCTATGTCGGCGACAAGACCGCGCGGCTTGTAAGCATCCTTGCGGCGGCAACGCTGGGCGCTGCCGCCGTAATGGTCG
CACCCGCGCTGACCAGTGGGGCGAGCGGGCCCGATACCGTCGCGTTCGGCGGCCAGTTCCTTGCGGACAGCTTTGCCAGC
TTTGCCAAGATCCTGATCTACCTTTCGGCCATAGGCTGCCTGATGATCGCCCCGGCCTTCTTCGACCGGCTCAAGGCCAT
GCGCCCGGAATATCCGGTGCTCGTCCTGCTGGCGACGCTGGGCATGAGCATCATGGTCTCCGCCGGGGATCTGATCACGC
TTTATATCGGCCTGGAGCTCAACAGCCTCGCGGCCTATGTCCTCGCCAGCTTCCTGCGCAACGACACGCGTTCGGCGGAG
GCAGGCCTCAAGTACTTCGTCCTCGGCGCGTTAGCCTCGGGTATCCTGCTGTACGGCATGAGCCTCGTCTACGGCTTTAC
CGGAACGACCGACTTCGAAGGCGTGCGTGGGGCGCTCACCGGCGACATGTCGACCGGCGCGCTGTTCGGGGTGATCTTCG
TGCTCGCCGGTCTCGCCTTCAAGATCGCCGCCGTGCCGTTCCACATGTGGACGCCGGACGTTTACGAAGGCGCGCCGACA
CCGGTGACGACATTCTTCGCCACCGCGCCCAAGGTGGCCGCCGTTGCCCTGACCGCGCGCGTCGCGCTCAGCCCGTTCGG
CGAGCAGACCGAGGCGTGGCAGCAGATCGTGATCTTCGCGGCGCTGGCCTCTATCGTACTCGGCGCGCTGGGCGCGATCG
GCCAGACCAACCTCAAGCGACTGCTGGCCTATTCCTCGATCAACAATGTCGGCTTCATCCTGATCGGCCTGGCCGCATCG
ACCGTCGCGGGCCTCAGCGCCATGCTGACCTATCTCGCGATCTATGTCGTGATGGCGCTGGGCAGTTTTGTGGCGCTGCT
GATGCTGAAGGATGAGGACGGCACCCCGCTCGAAACCTTCGACGATATTGCCGGGCTCTCGACGACGCGTCCGGCGCTCG
CATGGTGTCTGCTGTTCCTGATGTTCAGCCTCGCCGGCATCCCGCCGCTGCTGGGCTTCTGGGGCAAGTTCGTGGTCTTT
CAGGCCGCGGTGCAGGCCGACATGGTGCTGCTGGCTGCGCTCGGTATCGCTGCCAGCGTCATCGGTGCGTTCTACTACAT
CAAGTTCGTCAAGGTCATGTTCTTCGATGACGCGGTCGACCGCGTGAAGGGCACTTCGGATACGGCCCATTGGGTGCTGC
TGATATTGGCGGCGGTGGTCGTTTCGCCACTCGGTTACCTGCTGACGGGCTGGCTCGGCGGGCTGACCGATAGCGCCGCG
TCGGCGCTGTTCATCGCCTCTTGA

Upstream 100 bases:

>100_bases
GCGCCGCGCCGGAAGGCGATGCGAAACTGGCGGCGGGTGAGCCACGGGCGGAGGCCGCCAATGCCGTCGCTTACGAGGCC
AAGGAAGGGGGAGCGCACTG

Downstream 100 bases:

>100_bases
TTCAGACCGTCGCCGAAACCGGCTCGACTAACGCCGACCTGATTTCGCGCATCTGCGGTGGTGATCATGTGCCGGAAGGG
CACTGGCTGGTCGCAGACCG

Product: NADH dehydrogenase subunit N

Products: NA

Alternate protein names: NADH dehydrogenase I subunit N; NDH-1 subunit N

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MELNASLTLILPEIVMAISAMALILITAYVGDKTARLVSILAAATLGAAAVMVAPALTSGASGPDTVAFGGQFLADSFAS
FAKILIYLSAIGCLMIAPAFFDRLKAMRPEYPVLVLLATLGMSIMVSAGDLITLYIGLELNSLAAYVLASFLRNDTRSAE
AGLKYFVLGALASGILLYGMSLVYGFTGTTDFEGVRGALTGDMSTGALFGVIFVLAGLAFKIAAVPFHMWTPDVYEGAPT
PVTTFFATAPKVAAVALTARVALSPFGEQTEAWQQIVIFAALASIVLGALGAIGQTNLKRLLAYSSINNVGFILIGLAAS
TVAGLSAMLTYLAIYVVMALGSFVALLMLKDEDGTPLETFDDIAGLSTTRPALAWCLLFLMFSLAGIPPLLGFWGKFVVF
QAAVQADMVLLAALGIAASVIGAFYYIKFVKVMFFDDAVDRVKGTSDTAHWVLLILAAVVVSPLGYLLTGWLGGLTDSAA
SALFIAS

Sequences:

>Translated_487_residues
MELNASLTLILPEIVMAISAMALILITAYVGDKTARLVSILAAATLGAAAVMVAPALTSGASGPDTVAFGGQFLADSFAS
FAKILIYLSAIGCLMIAPAFFDRLKAMRPEYPVLVLLATLGMSIMVSAGDLITLYIGLELNSLAAYVLASFLRNDTRSAE
AGLKYFVLGALASGILLYGMSLVYGFTGTTDFEGVRGALTGDMSTGALFGVIFVLAGLAFKIAAVPFHMWTPDVYEGAPT
PVTTFFATAPKVAAVALTARVALSPFGEQTEAWQQIVIFAALASIVLGALGAIGQTNLKRLLAYSSINNVGFILIGLAAS
TVAGLSAMLTYLAIYVVMALGSFVALLMLKDEDGTPLETFDDIAGLSTTRPALAWCLLFLMFSLAGIPPLLGFWGKFVVF
QAAVQADMVLLAALGIAASVIGAFYYIKFVKVMFFDDAVDRVKGTSDTAHWVLLILAAVVVSPLGYLLTGWLGGLTDSAA
SALFIAS
>Mature_487_residues
MELNASLTLILPEIVMAISAMALILITAYVGDKTARLVSILAAATLGAAAVMVAPALTSGASGPDTVAFGGQFLADSFAS
FAKILIYLSAIGCLMIAPAFFDRLKAMRPEYPVLVLLATLGMSIMVSAGDLITLYIGLELNSLAAYVLASFLRNDTRSAE
AGLKYFVLGALASGILLYGMSLVYGFTGTTDFEGVRGALTGDMSTGALFGVIFVLAGLAFKIAAVPFHMWTPDVYEGAPT
PVTTFFATAPKVAAVALTARVALSPFGEQTEAWQQIVIFAALASIVLGALGAIGQTNLKRLLAYSSINNVGFILIGLAAS
TVAGLSAMLTYLAIYVVMALGSFVALLMLKDEDGTPLETFDDIAGLSTTRPALAWCLLFLMFSLAGIPPLLGFWGKFVVF
QAAVQADMVLLAALGIAASVIGAFYYIKFVKVMFFDDAVDRVKGTSDTAHWVLLILAAVVVSPLGYLLTGWLGGLTDSAA
SALFIAS

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1007

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 2 (chain N)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family

Homologues:

Organism=Homo sapiens, GI251831108, Length=313, Percent_Identity=25.5591054313099, Blast_Score=76, Evalue=7e-14,
Organism=Escherichia coli, GI145693160, Length=384, Percent_Identity=38.5416666666667, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI1788614, Length=357, Percent_Identity=24.9299719887955, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1788827, Length=317, Percent_Identity=28.0757097791798, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI2367154, Length=265, Percent_Identity=26.0377358490566, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788831, Length=301, Percent_Identity=22.5913621262458, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI1788829, Length=270, Percent_Identity=27.7777777777778, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NUON_ERYLH (Q2NA79)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_458209.1
- STRING:   Q2NA79
- GeneID:   3870384
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_06600
- NMPDR:   fig|314225.3.peg.1731
- HOGENOM:   HBG747830
- OMA:   EYPVLVL
- PhylomeDB:   Q2NA79
- ProtClustDB:   PRK05777
- BioCyc:   ELIT314225:ELI_06600-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_00445
- InterPro:   IPR010096
- InterPro:   IPR001750
- TIGRFAMs:   TIGR01770

Pfam domain/function: PF00361 Oxidored_q1

EC number: =1.6.99.5

Molecular weight: Translated: 50817; Mature: 50817

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x13b09cf0)-; HASH(0xce3d150)-; HASH(0xd9645a0)-; HASH(0xe1c5088)-; HASH(0xd82c948)-; HASH(0x14a6a668)-; HASH(0xe34f1bc)-; HASH(0xe0d675c)-; HASH(0xdd6e284)-; HASH(0x14b54494)-; HASH(0xd257728)-; HASH(0xe7a2940)-; HASH(0xe57e22c)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELNASLTLILPEIVMAISAMALILITAYVGDKTARLVSILAAATLGAAAVMVAPALTSG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ASGPDTVAFGGQFLADSFASFAKILIYLSAIGCLMIAPAFFDRLKAMRPEYPVLVLLATL
CCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
GMSIMVSAGDLITLYIGLELNSLAAYVLASFLRNDTRSAEAGLKYFVLGALASGILLYGM
HHHHEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
SLVYGFTGTTDFEGVRGALTGDMSTGALFGVIFVLAGLAFKIAAVPFHMWTPDVYEGAPT
HHHHCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCC
PVTTFFATAPKVAAVALTARVALSPFGEQTEAWQQIVIFAALASIVLGALGAIGQTNLKR
CHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LLAYSSINNVGFILIGLAASTVAGLSAMLTYLAIYVVMALGSFVALLMLKDEDGTPLETF
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHH
DDIAGLSTTRPALAWCLLFLMFSLAGIPPLLGFWGKFVVFQAAVQADMVLLAALGIAASV
HHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGAFYYIKFVKVMFFDDAVDRVKGTSDTAHWVLLILAAVVVSPLGYLLTGWLGGLTDSAA
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
SALFIAS
CEEEECH
>Mature Secondary Structure
MELNASLTLILPEIVMAISAMALILITAYVGDKTARLVSILAAATLGAAAVMVAPALTSG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ASGPDTVAFGGQFLADSFASFAKILIYLSAIGCLMIAPAFFDRLKAMRPEYPVLVLLATL
CCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
GMSIMVSAGDLITLYIGLELNSLAAYVLASFLRNDTRSAEAGLKYFVLGALASGILLYGM
HHHHEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
SLVYGFTGTTDFEGVRGALTGDMSTGALFGVIFVLAGLAFKIAAVPFHMWTPDVYEGAPT
HHHHCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCC
PVTTFFATAPKVAAVALTARVALSPFGEQTEAWQQIVIFAALASIVLGALGAIGQTNLKR
CHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LLAYSSINNVGFILIGLAASTVAGLSAMLTYLAIYVVMALGSFVALLMLKDEDGTPLETF
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHH
DDIAGLSTTRPALAWCLLFLMFSLAGIPPLLGFWGKFVVFQAAVQADMVLLAALGIAASV
HHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGAFYYIKFVKVMFFDDAVDRVKGTSDTAHWVLLILAAVVVSPLGYLLTGWLGGLTDSAA
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
SALFIAS
CEEEECH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA