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The map label for this gene is ycaI [H]

Identifier: 85374134

GI number: 85374134

Start: 1354714

End: 1356939

Strand: Reverse

Name: ycaI [H]

Synonym: ELI_06535

Alternate gene names: 85374134

Gene position: 1356939-1354714 (Counterclockwise)

Preceding gene: 85374138

Following gene: 85374122

Centisome position: 44.45

GC content: 64.91

Gene sequence:

>2226_bases
GTGGCTTCCTTTCCAATCGAGATCATGGCGACCGGTAGCGCCACTGATCCGCCCCCACTGATTCCGGTGGCAGGCGACCT
GGCGCAAGGTCATGTTGCATTGCAGCAACGCCCTTGGCATTCACGCTGGCGCTTGTCCAGCATAGGCGATTTCGCCGAAC
GTGCGCTGGGAGCTTCCGGCTTCGACAGAGCGCCGTGGATCGCGGTCAGTCTGTGTTTCGGCATAAGCCTGTGGTTCCTG
CTCGATAATCCATGGCAGTGGAGCGCTTTGATCGGTTCGTGTGGACTGGCTGCGCTCGGCGGTTGGCTGATGATCCGGCA
GACCGATCTCAAGCAATTCGGCATGGCTGTGGTGGCGGTTTCGCTTGCTGTCGGATTCGGGACAGGTCTCATCTGGGCCC
GGTCTGCCACGATCGGCGCGGAGGCGCTGCCGGCTCCGCAAGTGCAATGGTACCAGGGGAGGGTTCTCGAACGCGAGGAA
CAGCCTTCGCGCGACCGGATCAGGCTGGTGCTGGCTGTGCGCGATGCCGAAGCCGGAGAAGCACGCAAGATCCGCATAAA
TGTGCCACTGGAGCGGGCCCGGGATGACCTGCGCGAAAACGCGGTCGTCCGGTTTCGCGCGAGGTTGATGCCACCCGCAC
CGCCGATGGTGCCGGGGGCGTACAATTTCGCAAGAAGAGCGTGGTTCGATGGCTATGCTGCGACGGGATCGGTGCTCGGC
GAGATCGAATTGGTCGAACCTCCACCTCCACCCACCGGACTGGCTGCGGTGCAGCGCTTTCTTTCGGGCCACGTGCGCGA
CCGGGTTGATGGTTCGGCAGGGACTATCGCAGCGGCCTTTGCCAGCGGCGACAGGGGGGCGATTACGGAGGCGGACGAAG
AGGCGATGCGCGATGCAGGGCTGACGCACCTGCTTTCCATCAGCGGCTTGCATGTGAGCGCGATCATCGCCGCCGCTTAC
ATCCTCTCGCTCAAGCTCTTGGCGCTGTGGCCGTGGCTGGCCTTGCGGGTCCGACTGCCGCTGGTGGCTGCGGGCATCGG
CGCGCTGGCCGGGATCGGCTACACGCTCCTGACGGGTGCTGAAGTACCCACAGTGCGGAGCTGTGCGGCAGCGGTGCTGG
TGCTGATTGCACTCGCGCTCGGGCGCGAGCCGCTGTCGCTGCGGATGGTGGCAGTGGCAGCAGTGTTCGTGCTGCTGTTG
TGGCCGGAGTCGCTGGTCGGGCCGAGTTTCCAGATGAGCTTTGCGGCTGTGATCGCCATCGTGGCGCTGCACAATGCAGA
GCCGGTCAAACGCTTCCTCGCGCCGCGCGAAGAGGGCTGGTTTGCACATGTCGGTCGGCGCGCGCTGATGCTGCTCGTTA
CCGGGTTGGTGATCGAGATCGCTTTGATGCCGATCGTGCTGTTCCATTTCCATCGGGCTGGCGTCTACGGGGCGCTCGCC
AATGTCATTGCGATCCCATTGGTGACCTTCATTTCTATGCCGCTGATTGCGATAGCATTGGCGCTGGACGCTGTCGGACT
GGGTGAGCCGGTATGGTGGTTGGTCGGGCAGTCGCTCGATCTGCTGCTGGGCATTGCGCATTTCACTTCGAGCCAGCCAG
GCGCGGTCAAACTGATGCCGCAGATGGGGCTGGGGACGCTGTCGCTGTTCGTGGCGGGCGGATTGTGGCTCGCCTTGTGG
AAGGGACGCCGCCGATTGCTTGGCTTCGCACCGGTTGCGGCGGGGACCCTTCTGCTGCTGCTCACGCCAGTGCCCGACAT
CCTGATCTCTGGTGATGGTCGGCACGTGGGCATCGCCGAGGACAATCGGCTGATCATGCTGCGGGAGAGCCGATCGGACT
ATGCAAGGGACAACCTGATGGAGCTTGCCGGCATGTCGGGCGAGCCGGTGACGATTACGGACTGGCCGGGCGCACAGTGC
AGCAGGGACTTCTGCACGGTGACCATCCAGCGTGGCGGGCGGGACTGGCATGTGCTGATGGCGCGGAGCCGGGACCGGGT
CGAGGAGCGGGCGCTGGCGGCGGCATGCGAACGCTCCCACATCGTGATTGCCGATAGGTGGTTGCCCCGCTCTTGCCGGC
CGCAATGGCTCAAGGCGGATCGGCGGTTCCTGGGCCAGTCCGGGGGCCTGGCGCTGTTTCTGGAGGAGGAGCGGTTCACC
AGCGTGGCCCAGGGGGAGGGCGAGCATGGCTGGTGGAGAGGTCGGGAGAAGGGGGCGCGGCGGTAG

Upstream 100 bases:

>100_bases
GCGGTGCGCGCGCCGCCCAGGTGCAAATATCCGGTAGGTGATGGGGCGAAACGGGTGACGACATTACTCGTTCCAGCGTC
ATCTTTACTTGCCATCCTGC

Downstream 100 bases:

>100_bases
GCGGGGAAGCCTGCTGCGAGCGGGATCGCAGTTGCGGGGACAGTGCCGCGCCCCCTAGCTTGTGCAGATCAGTGGTATCG
GCGCAGCAAACCGGCGAGCT

Product: membrane metal-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 741; Mature: 740

Protein sequence:

>741_residues
MASFPIEIMATGSATDPPPLIPVAGDLAQGHVALQQRPWHSRWRLSSIGDFAERALGASGFDRAPWIAVSLCFGISLWFL
LDNPWQWSALIGSCGLAALGGWLMIRQTDLKQFGMAVVAVSLAVGFGTGLIWARSATIGAEALPAPQVQWYQGRVLEREE
QPSRDRIRLVLAVRDAEAGEARKIRINVPLERARDDLRENAVVRFRARLMPPAPPMVPGAYNFARRAWFDGYAATGSVLG
EIELVEPPPPPTGLAAVQRFLSGHVRDRVDGSAGTIAAAFASGDRGAITEADEEAMRDAGLTHLLSISGLHVSAIIAAAY
ILSLKLLALWPWLALRVRLPLVAAGIGALAGIGYTLLTGAEVPTVRSCAAAVLVLIALALGREPLSLRMVAVAAVFVLLL
WPESLVGPSFQMSFAAVIAIVALHNAEPVKRFLAPREEGWFAHVGRRALMLLVTGLVIEIALMPIVLFHFHRAGVYGALA
NVIAIPLVTFISMPLIAIALALDAVGLGEPVWWLVGQSLDLLLGIAHFTSSQPGAVKLMPQMGLGTLSLFVAGGLWLALW
KGRRRLLGFAPVAAGTLLLLLTPVPDILISGDGRHVGIAEDNRLIMLRESRSDYARDNLMELAGMSGEPVTITDWPGAQC
SRDFCTVTIQRGGRDWHVLMARSRDRVEERALAAACERSHIVIADRWLPRSCRPQWLKADRRFLGQSGGLALFLEEERFT
SVAQGEGEHGWWRGREKGARR

Sequences:

>Translated_741_residues
MASFPIEIMATGSATDPPPLIPVAGDLAQGHVALQQRPWHSRWRLSSIGDFAERALGASGFDRAPWIAVSLCFGISLWFL
LDNPWQWSALIGSCGLAALGGWLMIRQTDLKQFGMAVVAVSLAVGFGTGLIWARSATIGAEALPAPQVQWYQGRVLEREE
QPSRDRIRLVLAVRDAEAGEARKIRINVPLERARDDLRENAVVRFRARLMPPAPPMVPGAYNFARRAWFDGYAATGSVLG
EIELVEPPPPPTGLAAVQRFLSGHVRDRVDGSAGTIAAAFASGDRGAITEADEEAMRDAGLTHLLSISGLHVSAIIAAAY
ILSLKLLALWPWLALRVRLPLVAAGIGALAGIGYTLLTGAEVPTVRSCAAAVLVLIALALGREPLSLRMVAVAAVFVLLL
WPESLVGPSFQMSFAAVIAIVALHNAEPVKRFLAPREEGWFAHVGRRALMLLVTGLVIEIALMPIVLFHFHRAGVYGALA
NVIAIPLVTFISMPLIAIALALDAVGLGEPVWWLVGQSLDLLLGIAHFTSSQPGAVKLMPQMGLGTLSLFVAGGLWLALW
KGRRRLLGFAPVAAGTLLLLLTPVPDILISGDGRHVGIAEDNRLIMLRESRSDYARDNLMELAGMSGEPVTITDWPGAQC
SRDFCTVTIQRGGRDWHVLMARSRDRVEERALAAACERSHIVIADRWLPRSCRPQWLKADRRFLGQSGGLALFLEEERFT
SVAQGEGEHGWWRGREKGARR
>Mature_740_residues
ASFPIEIMATGSATDPPPLIPVAGDLAQGHVALQQRPWHSRWRLSSIGDFAERALGASGFDRAPWIAVSLCFGISLWFLL
DNPWQWSALIGSCGLAALGGWLMIRQTDLKQFGMAVVAVSLAVGFGTGLIWARSATIGAEALPAPQVQWYQGRVLEREEQ
PSRDRIRLVLAVRDAEAGEARKIRINVPLERARDDLRENAVVRFRARLMPPAPPMVPGAYNFARRAWFDGYAATGSVLGE
IELVEPPPPPTGLAAVQRFLSGHVRDRVDGSAGTIAAAFASGDRGAITEADEEAMRDAGLTHLLSISGLHVSAIIAAAYI
LSLKLLALWPWLALRVRLPLVAAGIGALAGIGYTLLTGAEVPTVRSCAAAVLVLIALALGREPLSLRMVAVAAVFVLLLW
PESLVGPSFQMSFAAVIAIVALHNAEPVKRFLAPREEGWFAHVGRRALMLLVTGLVIEIALMPIVLFHFHRAGVYGALAN
VIAIPLVTFISMPLIAIALALDAVGLGEPVWWLVGQSLDLLLGIAHFTSSQPGAVKLMPQMGLGTLSLFVAGGLWLALWK
GRRRLLGFAPVAAGTLLLLLTPVPDILISGDGRHVGIAEDNRLIMLRESRSDYARDNLMELAGMSGEPVTITDWPGAQCS
RDFCTVTIQRGGRDWHVLMARSRDRVEERALAAACERSHIVIADRWLPRSCRPQWLKADRRFLGQSGGLALFLEEERFTS
VAQGEGEHGWWRGREKGARR

Specific function: Unknown

COG id: COG0658

COG function: function code R; Predicted membrane metal-binding protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To B.subtilis ComEC, N.gonorrhoeae ComA, and H.influenzae Rec2 [H]

Homologues:

Organism=Escherichia coli, GI87081801, Length=244, Percent_Identity=29.0983606557377, Blast_Score=80, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR004477
- InterPro:   IPR004797 [H]

Pfam domain/function: PF03772 Competence; PF00753 Lactamase_B [H]

EC number: NA

Molecular weight: Translated: 80214; Mature: 80082

Theoretical pI: Translated: 8.87; Mature: 8.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASFPIEIMATGSATDPPPLIPVAGDLAQGHVALQQRPWHSRWRLSSIGDFAERALGASG
CCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHCCCCC
FDRAPWIAVSLCFGISLWFLLDNPWQWSALIGSCGLAALGGWLMIRQTDLKQFGMAVVAV
CCCCCHHHHHHHHHHHEEEEECCCCCHHHHHHCCCHHHHCCEEEEEEHHHHHHHHHHHHH
SLAVGFGTGLIWARSATIGAEALPAPQVQWYQGRVLEREEQPSRDRIRLVLAVRDAEAGE
HHHHHHCCCEEEEECCCCCCCCCCCCCCCHHCCCEECCCCCCCCCCEEEEEEEECCCCCC
ARKIRINVPLERARDDLRENAVVRFRARLMPPAPPMVPGAYNFARRAWFDGYAATGSVLG
CEEEEEECCHHHHHHHHHHHHEEEHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEE
EIELVEPPPPPTGLAAVQRFLSGHVRDRVDGSAGTIAAAFASGDRGAITEADEEAMRDAG
EEEEECCCCCCCHHHHHHHHHHCHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHCC
LTHLLSISGLHVSAIIAAAYILSLKLLALWPWLALRVRLPLVAAGIGALAGIGYTLLTGA
CHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCC
EVPTVRSCAAAVLVLIALALGREPLSLRMVAVAAVFVLLLWPESLVGPSFQMSFAAVIAI
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHH
VALHNAEPVKRFLAPREEGWFAHVGRRALMLLVTGLVIEIALMPIVLFHFHRAGVYGALA
HHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NVIAIPLVTFISMPLIAIALALDAVGLGEPVWWLVGQSLDLLLGIAHFTSSQPGAVKLMP
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHCCCCCCEEEECC
QMGLGTLSLFVAGGLWLALWKGRRRLLGFAPVAAGTLLLLLTPVPDILISGDGRHVGIAE
CCCCHHHHHHHHHHHHHHHHHCCHHHEEECHHHHHHHHHEECCCCCEEECCCCCEEEEEC
DNRLIMLRESRSDYARDNLMELAGMSGEPVTITDWPGAQCSRDFCTVTIQRGGRDWHVLM
CCEEEEEECCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCEEEEEEECCCCEEEEEE
ARSRDRVEERALAAACERSHIVIADRWLPRSCRPQWLKADRRFLGQSGGLALFLEEERFT
ECCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEEHHHHH
SVAQGEGEHGWWRGREKGARR
HHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ASFPIEIMATGSATDPPPLIPVAGDLAQGHVALQQRPWHSRWRLSSIGDFAERALGASG
CCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHCCCCC
FDRAPWIAVSLCFGISLWFLLDNPWQWSALIGSCGLAALGGWLMIRQTDLKQFGMAVVAV
CCCCCHHHHHHHHHHHEEEEECCCCCHHHHHHCCCHHHHCCEEEEEEHHHHHHHHHHHHH
SLAVGFGTGLIWARSATIGAEALPAPQVQWYQGRVLEREEQPSRDRIRLVLAVRDAEAGE
HHHHHHCCCEEEEECCCCCCCCCCCCCCCHHCCCEECCCCCCCCCCEEEEEEEECCCCCC
ARKIRINVPLERARDDLRENAVVRFRARLMPPAPPMVPGAYNFARRAWFDGYAATGSVLG
CEEEEEECCHHHHHHHHHHHHEEEHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEE
EIELVEPPPPPTGLAAVQRFLSGHVRDRVDGSAGTIAAAFASGDRGAITEADEEAMRDAG
EEEEECCCCCCCHHHHHHHHHHCHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHCC
LTHLLSISGLHVSAIIAAAYILSLKLLALWPWLALRVRLPLVAAGIGALAGIGYTLLTGA
CHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCC
EVPTVRSCAAAVLVLIALALGREPLSLRMVAVAAVFVLLLWPESLVGPSFQMSFAAVIAI
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHH
VALHNAEPVKRFLAPREEGWFAHVGRRALMLLVTGLVIEIALMPIVLFHFHRAGVYGALA
HHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NVIAIPLVTFISMPLIAIALALDAVGLGEPVWWLVGQSLDLLLGIAHFTSSQPGAVKLMP
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHCCCCCCEEEECC
QMGLGTLSLFVAGGLWLALWKGRRRLLGFAPVAAGTLLLLLTPVPDILISGDGRHVGIAE
CCCCHHHHHHHHHHHHHHHHHCCHHHEEECHHHHHHHHHEECCCCCEEECCCCCEEEEEC
DNRLIMLRESRSDYARDNLMELAGMSGEPVTITDWPGAQCSRDFCTVTIQRGGRDWHVLM
CCEEEEEECCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCEEEEEEECCCCEEEEEE
ARSRDRVEERALAAACERSHIVIADRWLPRSCRPQWLKADRRFLGQSGGLALFLEEERFT
ECCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEEHHHHH
SVAQGEGEHGWWRGREKGARR
HHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 8094880 [H]