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The map label for this gene is pleC [H]

Identifier: 85374138

GI number: 85374138

Start: 1359913

End: 1361325

Strand: Reverse

Name: pleC [H]

Synonym: ELI_06555

Alternate gene names: 85374138

Gene position: 1361325-1359913 (Counterclockwise)

Preceding gene: 85374139

Following gene: 85374134

Centisome position: 44.6

GC content: 61.71

Gene sequence:

>1413_bases
ATGGCGGGTGTCCTGACAGTGGATCGACGCAAATCCACAGTTCTTGCCAAAGCGCGCACTGACGCCGGGGATCGTCTCGT
CGAAGCGGATGACCCGCTCGCGAGCCTGCAATCCAGGTGTGGAGGGGAAATTCCGGGCACCATTGCGATCCATGAACTGA
GGCAGCTCGTACGCAAGGCGCGAGAGATGGATCTGCGTCTTGCGCGGACCATTCGCGCATTCGATGGTGAGTCCCGCATC
ACGGCCTGGGTCGAAGTCGCTCCTTATAACGGCGAAGTGGAGCAAGGATGTGCGATCACCGTGCAGACATGGCAAGTGGC
GGTGCCGCAAGCCGAAAGTGAAGACGCCGCAGCTGCCCGGCGCGATGCGATCGACAATGCCCTCGCCGAATTGCATGCGC
GGCTCGATGCCGAACAGCGTATCATCACGATCGACACCGACGCCGACGACCTCCAGCCGCTGGTCAAGGCGATCAAGGCC
GACCCCGGACGGCGCTGGACCGACTATGTTTCGCTGCCCGGCATCGCTCATGCCCAGCCATTCCATTGGCGCCTTCTCGA
CCAGGCCCGCTGCGAGATCGAGGGCAGCCCACGGCAATGGACGGCGCGGCTCATTCCCATGGGGACACCCGAACCGGGGA
GTGAAGGCTTCGAGCTCTATCTCGTCGCCAACCGGGCCTGGGTTCAGCGATCCGACCGGAGGCGTGGGCAAAACAAGGAA
GTGCAGGCTCGCATGGGGCGCGATCTCTCGCCTGTGCTCCGCCAACCGGTTTCGCGGATCATCGCAAATGCCGAGACAAT
CCGTACCAAGCTCGCCGGTCCGCTTGCCGACGAGTATAGCGACTATGCCGCAGACATCGCCAATGCGGGGCAACATCTCC
TTTCGCTGATCGACGATCTTGCCGATCTTGAGATCATCGAATCCGAAGGCTTTTCTACCGCCCCGGACAAGATCGACCTG
GTGCAAGTGGTGCGGCAGGCCGCGGGAATTCTCGGCGTGACGGCGCAGGAAAAACAGATCGAAATACTCGTCCCGGACAA
GGATCAGTCATGCCCCGCGATCGGCGAATTCCGGAGGGTATTGCAGGTCCTGCTGAATCTGATCGGTAATGCCATCCGCT
ATTCGCCTGTCGGCTCCAGGATCCGCGTCGAACTCGCACGCAATGGAAAGGTTGCCGGCGCGCGGGTCATCGACGAAGGC
CCCGGCATCGACACGGAGCAACAGGCGGCGATCTTCGACAAATTCGAACGTCTCGGCCGCTCCGGCGATGGTGGCTCCGG
CCTTGGCCTCTATATCTCGCGCCGCCTCGCACGGGCGATGGGTGGCGACCTGAAAGTTAAAAGCACGCGCGGCGAAGGCG
CAACGTTTACGCTTACATTGCCGGTTTCGCCTGCAAAAAGATCAAAGCCCTAA

Upstream 100 bases:

>100_bases
AGGAGCAATTCCTCTATATTCATCCCGAGGTATCACTGCCCGAAGGATTTTCCCAGTTGAGGGTCGAGCGGGCGCAGGCG
ATGCTTGCAGCCTCGCCCGG

Downstream 100 bases:

>100_bases
CGGCGAAAGCGGCGCGACAGACTCAGAGCCACCCCGCCCAGTGCAAACACTCCCGCACCGATATAGGCCCACTGCTGCTT
GCCGGCGATATAGCTGGTTT

Product: two-component signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MAGVLTVDRRKSTVLAKARTDAGDRLVEADDPLASLQSRCGGEIPGTIAIHELRQLVRKAREMDLRLARTIRAFDGESRI
TAWVEVAPYNGEVEQGCAITVQTWQVAVPQAESEDAAAARRDAIDNALAELHARLDAEQRIITIDTDADDLQPLVKAIKA
DPGRRWTDYVSLPGIAHAQPFHWRLLDQARCEIEGSPRQWTARLIPMGTPEPGSEGFELYLVANRAWVQRSDRRRGQNKE
VQARMGRDLSPVLRQPVSRIIANAETIRTKLAGPLADEYSDYAADIANAGQHLLSLIDDLADLEIIESEGFSTAPDKIDL
VQVVRQAAGILGVTAQEKQIEILVPDKDQSCPAIGEFRRVLQVLLNLIGNAIRYSPVGSRIRVELARNGKVAGARVIDEG
PGIDTEQQAAIFDKFERLGRSGDGGSGLGLYISRRLARAMGGDLKVKSTRGEGATFTLTLPVSPAKRSKP

Sequences:

>Translated_470_residues
MAGVLTVDRRKSTVLAKARTDAGDRLVEADDPLASLQSRCGGEIPGTIAIHELRQLVRKAREMDLRLARTIRAFDGESRI
TAWVEVAPYNGEVEQGCAITVQTWQVAVPQAESEDAAAARRDAIDNALAELHARLDAEQRIITIDTDADDLQPLVKAIKA
DPGRRWTDYVSLPGIAHAQPFHWRLLDQARCEIEGSPRQWTARLIPMGTPEPGSEGFELYLVANRAWVQRSDRRRGQNKE
VQARMGRDLSPVLRQPVSRIIANAETIRTKLAGPLADEYSDYAADIANAGQHLLSLIDDLADLEIIESEGFSTAPDKIDL
VQVVRQAAGILGVTAQEKQIEILVPDKDQSCPAIGEFRRVLQVLLNLIGNAIRYSPVGSRIRVELARNGKVAGARVIDEG
PGIDTEQQAAIFDKFERLGRSGDGGSGLGLYISRRLARAMGGDLKVKSTRGEGATFTLTLPVSPAKRSKP
>Mature_469_residues
AGVLTVDRRKSTVLAKARTDAGDRLVEADDPLASLQSRCGGEIPGTIAIHELRQLVRKAREMDLRLARTIRAFDGESRIT
AWVEVAPYNGEVEQGCAITVQTWQVAVPQAESEDAAAARRDAIDNALAELHARLDAEQRIITIDTDADDLQPLVKAIKAD
PGRRWTDYVSLPGIAHAQPFHWRLLDQARCEIEGSPRQWTARLIPMGTPEPGSEGFELYLVANRAWVQRSDRRRGQNKEV
QARMGRDLSPVLRQPVSRIIANAETIRTKLAGPLADEYSDYAADIANAGQHLLSLIDDLADLEIIESEGFSTAPDKIDLV
QVVRQAAGILGVTAQEKQIEILVPDKDQSCPAIGEFRRVLQVLLNLIGNAIRYSPVGSRIRVELARNGKVAGARVIDEGP
GIDTEQQAAIFDKFERLGRSGDGGSGLGLYISRRLARAMGGDLKVKSTRGEGATFTLTLPVSPAKRSKP

Specific function: Member of the two-component regulatory system involved in the regulation of polar organelle development. PleC functions as a membrane-associated protein kinase that transfers phosphate to the response regulator pleD, leading to its activation [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788713, Length=246, Percent_Identity=30.0813008130081, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI87081816, Length=227, Percent_Identity=31.2775330396476, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI48994928, Length=220, Percent_Identity=29.5454545454545, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI145693157, Length=218, Percent_Identity=29.3577981651376, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786912, Length=240, Percent_Identity=31.25, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1789149, Length=232, Percent_Identity=27.5862068965517, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87082128, Length=220, Percent_Identity=26.8181818181818, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788549, Length=244, Percent_Identity=27.8688524590164, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788393, Length=231, Percent_Identity=23.3766233766234, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1787894, Length=142, Percent_Identity=35.2112676056338, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1790436, Length=216, Percent_Identity=28.2407407407407, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1786600, Length=177, Percent_Identity=28.8135593220339, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 51359; Mature: 51228

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGVLTVDRRKSTVLAKARTDAGDRLVEADDPLASLQSRCGGEIPGTIAIHELRQLVRKA
CCCCEEECCCCCCEEEECCCCCCCCEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
REMDLRLARTIRAFDGESRITAWVEVAPYNGEVEQGCAITVQTWQVAVPQAESEDAAAAR
HHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCEEEEEEEEEEECCCCCCCHHHHHH
RDAIDNALAELHARLDAEQRIITIDTDADDLQPLVKAIKADPGRRWTDYVSLPGIAHAQP
HHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCC
FHWRLLDQARCEIEGSPRQWTARLIPMGTPEPGSEGFELYLVANRAWVQRSDRRRGQNKE
CHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCEEEEEEECHHHHHHHHHHCCCCCH
VQARMGRDLSPVLRQPVSRIIANAETIRTKLAGPLADEYSDYAADIANAGQHLLSLIDDL
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ADLEIIESEGFSTAPDKIDLVQVVRQAAGILGVTAQEKQIEILVPDKDQSCPAIGEFRRV
HHHEEEECCCCCCCCCHHHHHHHHHHHCCEEEECCCCCEEEEEECCCCCCCCCHHHHHHH
LQVLLNLIGNAIRYSPVGSRIRVELARNGKVAGARVIDEGPGIDTEQQAAIFDKFERLGR
HHHHHHHHCCHHEECCCCCEEEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCC
SGDGGSGLGLYISRRLARAMGGDLKVKSTRGEGATFTLTLPVSPAKRSKP
CCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEEECCCCCCCCC
>Mature Secondary Structure 
AGVLTVDRRKSTVLAKARTDAGDRLVEADDPLASLQSRCGGEIPGTIAIHELRQLVRKA
CCCEEECCCCCCEEEECCCCCCCCEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
REMDLRLARTIRAFDGESRITAWVEVAPYNGEVEQGCAITVQTWQVAVPQAESEDAAAAR
HHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCEEEEEEEEEEECCCCCCCHHHHHH
RDAIDNALAELHARLDAEQRIITIDTDADDLQPLVKAIKADPGRRWTDYVSLPGIAHAQP
HHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCC
FHWRLLDQARCEIEGSPRQWTARLIPMGTPEPGSEGFELYLVANRAWVQRSDRRRGQNKE
CHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCEEEEEEECHHHHHHHHHHCCCCCH
VQARMGRDLSPVLRQPVSRIIANAETIRTKLAGPLADEYSDYAADIANAGQHLLSLIDDL
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ADLEIIESEGFSTAPDKIDLVQVVRQAAGILGVTAQEKQIEILVPDKDQSCPAIGEFRRV
HHHEEEECCCCCCCCCHHHHHHHHHHHCCEEEECCCCCEEEEEECCCCCCCCCHHHHHHH
LQVLLNLIGNAIRYSPVGSRIRVELARNGKVAGARVIDEGPGIDTEQQAAIFDKFERLGR
HHHHHHHHCCHHEECCCCCEEEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCC
SGDGGSGLGLYISRRLARAMGGDLKVKSTRGEGATFTLTLPVSPAKRSKP
CCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8421698; 11259647 [H]