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The map label for this gene is pyrG

Identifier: 85374119

GI number: 85374119

Start: 1340540

End: 1342174

Strand: Reverse

Name: pyrG

Synonym: ELI_06460

Alternate gene names: 85374119

Gene position: 1342174-1340540 (Counterclockwise)

Preceding gene: 85374120

Following gene: 85374117

Centisome position: 43.97

GC content: 63.24

Gene sequence:

>1635_bases
ATGGCGCGGTATATTTTCATCACCGGCGGCGTGGTCTCCTCGCTCGGAAAAGGTCTCATGGCGGCATCGCTCGGCGCGCT
GCTGCAGGCGCGCGGCTACAAGGTCCGCATCCGTAAGTTCGACCCCTATCTGAACGTCGATCCGGGCACGATGAGCCCGT
ACCAGCACGGCGAGGTCTATGTGACCGACGACGGGGCCGAGACGGATCTCGACTTAGGGCACTACGAGCGCTTCACCGGC
GTTTCTGCGCGCCAGAGCGACAACATCACCTCGGGCCGGGTCTATCAGGACATCATCGCCAAGGAACGCCGCGGTGACTA
CCTCGGCGCCACGGTGCAGGTGATCCCGCACGTTACCGACGCGATCAAGAATTTCGCGCTCGCCGACCAGGACGATCTCG
ATTTCATCCTTTGCGAGATCGGCGGGACCGTGGGCGACATCGAGTCCCTGCCTTTCATGGAGGCGATCCGCCAGCTCAGG
AACGAGCTGGAGCCGATGCAGACGCTCAGCGTCCACGTGACGCTGGTACCCTACATTGCGGCTGCGGGTGAGTTGAAGAC
CAAGCCGACCCAGCATTCGGTACGCGAACTCGCGAGCCTCGGGATCAAGCCCGATGTGCTGCTCTGCCGCGCCGAACATC
CGCTGCCCGATGGCGAGCGGCAGAAGATCGCCAATTTCTGCAACGTACGCAAAGAAGCCGTGATCCCCGCGCTTGACGCA
CCGTCGATCTATTCGGTGCCGCTGCAATATCACGAGGAAGGCCTCGATGCCGAGGTTTTGCGCGGCTTCGGAATCGTGGA
TGCGCCTGCGCCCGACCTGTCGGCATGGGACGATGTTACCGACCGTTATTTCAATCCCGAAGGCGAAGTGACGATCGGCG
TGGTCGGCAAATATGTCGGCCTCCAGGATGCGTACAAGTCGCTGAACGAGGCACTGGTCCACGGCGGGCTCGCCAACCGC
GTGAAGGTCAATATCCGCTGGATCGATGCCGAGGTCTTCGAAGAGGACGATGCGGCGATCGCCGCCAAGCTCGAGCCCCT
GCACGGGATCCTCGTACCCGGCGGCTTCGGCGAACGGGGCAGTGAAGGCAAGATCGCCAGCGTGCGCTTCGCGCGCGAAC
GCAAAGTGCCCTTCTTCGGCATTTGTCTCGGCATGCAGATGGCCTGCATCGAAGGCGCGCGCGAGGCGGGGCACGCGCTA
GCGTCCTCGACCGAATTCGGCGAAACCGAGGAGCCGGTCGTCGGCATCATCACCGAATGGATGACCGAGGAGGGCCTCGA
AAAGCGTGAAGCAGGGGGCGATCTCGGTGGCACGATGCGGCTGGGAGCGTACGAAGCCAAGCTCGACGGCAACAGCCACG
TGAGCGCGATCTACGGCGGCGCCACCGAGATTTCCGAGCGGCACCGCCATCGCTACGAGGTCAATGCGGCCTATCGCGAT
GCGCTGGAAGGGCAGGGGTTGATCTTTTCAGGCATGTCGCCCGACGGCCTTCTGCCCGAAATCGTCGAACGGCCCGATCA
CCCCTGGTTCGTCGGCGTGCAGTTCCATCCGGAGCTCAAATCCAAGCCATTCGACCCGCACCCGCTGTTTTCCGGCTTCA
TCAAGGCCGCGCTGCACCAGTCCCGTCTGGTCTAG

Upstream 100 bases:

>100_bases
ACCGAATAAGCTCCAAATAGCGCAGACATGTGGATTGCGGGCGTTTGCCTGCCCAATCCGCTTGCGCCGAATCCAGTTTC
ACGACTAAGGCCCAACTCCC

Downstream 100 bases:

>100_bases
GCAATTAACCTAGTCGCTTTACCCGCGAATCTGTCGCGTTTCTGCAAGTTTCGGTATGGCGGACCTACTAAAGTAGGCCA
ATGGTTACCATTTTTACCGA

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase

Number of amino acids: Translated: 544; Mature: 543

Protein sequence:

>544_residues
MARYIFITGGVVSSLGKGLMAASLGALLQARGYKVRIRKFDPYLNVDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFTG
VSARQSDNITSGRVYQDIIAKERRGDYLGATVQVIPHVTDAIKNFALADQDDLDFILCEIGGTVGDIESLPFMEAIRQLR
NELEPMQTLSVHVTLVPYIAAAGELKTKPTQHSVRELASLGIKPDVLLCRAEHPLPDGERQKIANFCNVRKEAVIPALDA
PSIYSVPLQYHEEGLDAEVLRGFGIVDAPAPDLSAWDDVTDRYFNPEGEVTIGVVGKYVGLQDAYKSLNEALVHGGLANR
VKVNIRWIDAEVFEEDDAAIAAKLEPLHGILVPGGFGERGSEGKIASVRFARERKVPFFGICLGMQMACIEGAREAGHAL
ASSTEFGETEEPVVGIITEWMTEEGLEKREAGGDLGGTMRLGAYEAKLDGNSHVSAIYGGATEISERHRHRYEVNAAYRD
ALEGQGLIFSGMSPDGLLPEIVERPDHPWFVGVQFHPELKSKPFDPHPLFSGFIKAALHQSRLV

Sequences:

>Translated_544_residues
MARYIFITGGVVSSLGKGLMAASLGALLQARGYKVRIRKFDPYLNVDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFTG
VSARQSDNITSGRVYQDIIAKERRGDYLGATVQVIPHVTDAIKNFALADQDDLDFILCEIGGTVGDIESLPFMEAIRQLR
NELEPMQTLSVHVTLVPYIAAAGELKTKPTQHSVRELASLGIKPDVLLCRAEHPLPDGERQKIANFCNVRKEAVIPALDA
PSIYSVPLQYHEEGLDAEVLRGFGIVDAPAPDLSAWDDVTDRYFNPEGEVTIGVVGKYVGLQDAYKSLNEALVHGGLANR
VKVNIRWIDAEVFEEDDAAIAAKLEPLHGILVPGGFGERGSEGKIASVRFARERKVPFFGICLGMQMACIEGAREAGHAL
ASSTEFGETEEPVVGIITEWMTEEGLEKREAGGDLGGTMRLGAYEAKLDGNSHVSAIYGGATEISERHRHRYEVNAAYRD
ALEGQGLIFSGMSPDGLLPEIVERPDHPWFVGVQFHPELKSKPFDPHPLFSGFIKAALHQSRLV
>Mature_543_residues
ARYIFITGGVVSSLGKGLMAASLGALLQARGYKVRIRKFDPYLNVDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFTGV
SARQSDNITSGRVYQDIIAKERRGDYLGATVQVIPHVTDAIKNFALADQDDLDFILCEIGGTVGDIESLPFMEAIRQLRN
ELEPMQTLSVHVTLVPYIAAAGELKTKPTQHSVRELASLGIKPDVLLCRAEHPLPDGERQKIANFCNVRKEAVIPALDAP
SIYSVPLQYHEEGLDAEVLRGFGIVDAPAPDLSAWDDVTDRYFNPEGEVTIGVVGKYVGLQDAYKSLNEALVHGGLANRV
KVNIRWIDAEVFEEDDAAIAAKLEPLHGILVPGGFGERGSEGKIASVRFARERKVPFFGICLGMQMACIEGAREAGHALA
SSTEFGETEEPVVGIITEWMTEEGLEKREAGGDLGGTMRLGAYEAKLDGNSHVSAIYGGATEISERHRHRYEVNAAYRDA
LEGQGLIFSGMSPDGLLPEIVERPDHPWFVGVQFHPELKSKPFDPHPLFSGFIKAALHQSRLV

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI148491070, Length=561, Percent_Identity=45.0980392156863, Blast_Score=471, Evalue=1e-133,
Organism=Homo sapiens, GI28559085, Length=559, Percent_Identity=43.1127012522361, Blast_Score=447, Evalue=1e-126,
Organism=Homo sapiens, GI28559083, Length=559, Percent_Identity=43.1127012522361, Blast_Score=447, Evalue=1e-126,
Organism=Homo sapiens, GI221316689, Length=559, Percent_Identity=43.1127012522361, Blast_Score=447, Evalue=1e-126,
Organism=Escherichia coli, GI1789142, Length=539, Percent_Identity=57.5139146567718, Blast_Score=583, Evalue=1e-167,
Organism=Caenorhabditis elegans, GI25148299, Length=610, Percent_Identity=37.7049180327869, Blast_Score=384, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6322563, Length=569, Percent_Identity=41.6520210896309, Blast_Score=417, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6319432, Length=576, Percent_Identity=40.4513888888889, Blast_Score=412, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24664469, Length=557, Percent_Identity=44.165170556553, Blast_Score=435, Evalue=1e-122,
Organism=Drosophila melanogaster, GI21357815, Length=503, Percent_Identity=42.9423459244533, Blast_Score=368, Evalue=1e-102,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRG_ERYLH (Q2NAA7)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_458181.1
- HSSP:   P17812
- ProteinModelPortal:   Q2NAA7
- STRING:   Q2NAA7
- GeneID:   3869557
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_06460
- eggNOG:   COG0504
- HOGENOM:   HBG597806
- OMA:   TEWLRGN
- PhylomeDB:   Q2NAA7
- ProtClustDB:   PRK05380
- BioCyc:   ELIT314225:ELI_06460-MONOMER
- HAMAP:   MF_01227
- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991
- TIGRFAMs:   TIGR00337

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase

EC number: =6.3.4.2

Molecular weight: Translated: 59465; Mature: 59334

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 382-382 ACT_SITE 516-516 ACT_SITE 518-518

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARYIFITGGVVSSLGKGLMAASLGALLQARGYKVRIRKFDPYLNVDPGTMSPYQHGEVY
CCEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCEEECCCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFTGVSARQSDNITSGRVYQDIIAKERRGDYLGATVQVIPHVTD
EECCCCCCCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHH
AIKNFALADQDDLDFILCEIGGTVGDIESLPFMEAIRQLRNELEPMQTLSVHVTLVPYIA
HHHHCCCCCCCCCCEEEEECCCCCCCHHCCCHHHHHHHHHHHCCHHHEEEEEEEEEHHHH
AAGELKTKPTQHSVRELASLGIKPDVLLCRAEHPLPDGERQKIANFCNVRKEAVIPALDA
HCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
PSIYSVPLQYHEEGLDAEVLRGFGIVDAPAPDLSAWDDVTDRYFNPEGEVTIGVVGKYVG
CCEEECCHHHHHCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEHHHHCC
LQDAYKSLNEALVHGGLANRVKVNIRWIDAEVFEEDDAAIAAKLEPLHGILVPGGFGERG
HHHHHHHHHHHHHHCCCCCEEEEEEEEEEHHHHCCCCCEEEEEECCCCEEEECCCCCCCC
SEGKIASVRFARERKVPFFGICLGMQMACIEGAREAGHALASSTEFGETEEPVVGIITEW
CCCCEEEEEEHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
MTEEGLEKREAGGDLGGTMRLGAYEAKLDGNSHVSAIYGGATEISERHRHRYEVNAAYRD
HHHHCHHHHHCCCCCCCEEEECEEEEECCCCCCEEEEECCHHHHHHHHHHHEEECHHHHH
ALEGQGLIFSGMSPDGLLPEIVERPDHPWFVGVQFHPELKSKPFDPHPLFSGFIKAALHQ
HCCCCCEEEECCCCCCCCHHHHCCCCCCEEEEEEECCHHCCCCCCCCHHHHHHHHHHHHH
SRLV
HCCC
>Mature Secondary Structure 
ARYIFITGGVVSSLGKGLMAASLGALLQARGYKVRIRKFDPYLNVDPGTMSPYQHGEVY
CEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCEEECCCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFTGVSARQSDNITSGRVYQDIIAKERRGDYLGATVQVIPHVTD
EECCCCCCCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHH
AIKNFALADQDDLDFILCEIGGTVGDIESLPFMEAIRQLRNELEPMQTLSVHVTLVPYIA
HHHHCCCCCCCCCCEEEEECCCCCCCHHCCCHHHHHHHHHHHCCHHHEEEEEEEEEHHHH
AAGELKTKPTQHSVRELASLGIKPDVLLCRAEHPLPDGERQKIANFCNVRKEAVIPALDA
HCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
PSIYSVPLQYHEEGLDAEVLRGFGIVDAPAPDLSAWDDVTDRYFNPEGEVTIGVVGKYVG
CCEEECCHHHHHCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEHHHHCC
LQDAYKSLNEALVHGGLANRVKVNIRWIDAEVFEEDDAAIAAKLEPLHGILVPGGFGERG
HHHHHHHHHHHHHHCCCCCEEEEEEEEEEHHHHCCCCCEEEEEECCCCEEEECCCCCCCC
SEGKIASVRFARERKVPFFGICLGMQMACIEGAREAGHALASSTEFGETEEPVVGIITEW
CCCCEEEEEEHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
MTEEGLEKREAGGDLGGTMRLGAYEAKLDGNSHVSAIYGGATEISERHRHRYEVNAAYRD
HHHHCHHHHHCCCCCCCEEEECEEEEECCCCCCEEEEECCHHHHHHHHHHHEEECHHHHH
ALEGQGLIFSGMSPDGLLPEIVERPDHPWFVGVQFHPELKSKPFDPHPLFSGFIKAALHQ
HCCCCCEEEECCCCCCCCHHHHCCCCCCEEEEEEECCHHCCCCCCCCHHHHHHHHHHHHH
SRLV
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA