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The map label for this gene is yafV [C]

Identifier: 85374116

GI number: 85374116

Start: 1338404

End: 1339207

Strand: Reverse

Name: yafV [C]

Synonym: ELI_06445

Alternate gene names: 85374116

Gene position: 1339207-1338404 (Counterclockwise)

Preceding gene: 85374117

Following gene: 85374115

Centisome position: 43.87

GC content: 63.43

Gene sequence:

>804_bases
ATGACCTCGGGGATCGATCCTGAGGCCAATTTCGCCGCGATTGAGCAGGCGGCGCGGGAAGCGAGTGCGGGCGGGGCGGA
AATGCTGTTCACGCCTGAGATGAGCCTGTTGCTCGATCGCGATCGCAAGCGCGCGGCGCAGCATATCGCTCCGCAGGCCC
AATCCCCATACCCTGAGCGGCTGCAAGCCATAGCCCGTGAGGCAGGGCTGTGGCTGACTGTGGGCGCACCGGTCAGGGTC
GAAGACGATCGTTACGCCAATCGACAATGGTTGTTCTACCCGAGGGGCGAGAGCCCAGTGCATTACGACAAGATCCACAT
GTTCGATGTCGATCTTGCCGATGGCGAGAGTTGGCGTGAATCGAACGCCTACCGTCCGGGTGAGGAAGTCGTATTGGTCG
ACGACACGCCCGTGGGACGGCTTGGCCTGACGATTTGCTATGACCTGCGCTTTCCGGCGCTATTTGAAGAGCTGGGCAAT
GCGCGGGCCGATTGCATCGCGATCCCTGCGGCTTTCACGCGACCGACCGGCGCGGCGCATTGGCACACGCTGGTCCGCGC
CCGCGCCATCGAAGCGAGCGCCTTCGTTGTCGCAGCGGCGCAGGTCGGCGAGCACGAAGACGGTCGAAAAACTTATGGTC
ACAGCCTGGTAGTCGATCCGTGGGGCGAGGTCCTGCTCGACATGAGCGGAGACAAACCGGGCCTGGGCTTCGCCGATATC
GATTTGGCGCGCATCGAGGAAGTGCGGCGACAGGTCCCCAGCCTTGCCAACCGCCGCCCGATCCCTCAATCCACCGCGCA
ATGA

Upstream 100 bases:

>100_bases
GTTCCGACGAGCTTCACGCGTTGGAGCGTGAAGGCAAACTCGATCCGCTGCTGGCAGGCTGAGGGCAGTCAGGTCGATGC
CTAGGATAGCCGTCCTGCAA

Downstream 100 bases:

>100_bases
TCGTCTACGACCTTTCCTGCTCGAGCAGCCATCGGTTCGAGGTCTGGTTCCGCTCCAGCGCGGACTTTGCCGAGCAGCGC
GAGAAGGGCTGGCTGCAATG

Product: nitrilase 1 like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MTSGIDPEANFAAIEQAAREASAGGAEMLFTPEMSLLLDRDRKRAAQHIAPQAQSPYPERLQAIAREAGLWLTVGAPVRV
EDDRYANRQWLFYPRGESPVHYDKIHMFDVDLADGESWRESNAYRPGEEVVLVDDTPVGRLGLTICYDLRFPALFEELGN
ARADCIAIPAAFTRPTGAAHWHTLVRARAIEASAFVVAAAQVGEHEDGRKTYGHSLVVDPWGEVLLDMSGDKPGLGFADI
DLARIEEVRRQVPSLANRRPIPQSTAQ

Sequences:

>Translated_267_residues
MTSGIDPEANFAAIEQAAREASAGGAEMLFTPEMSLLLDRDRKRAAQHIAPQAQSPYPERLQAIAREAGLWLTVGAPVRV
EDDRYANRQWLFYPRGESPVHYDKIHMFDVDLADGESWRESNAYRPGEEVVLVDDTPVGRLGLTICYDLRFPALFEELGN
ARADCIAIPAAFTRPTGAAHWHTLVRARAIEASAFVVAAAQVGEHEDGRKTYGHSLVVDPWGEVLLDMSGDKPGLGFADI
DLARIEEVRRQVPSLANRRPIPQSTAQ
>Mature_266_residues
TSGIDPEANFAAIEQAAREASAGGAEMLFTPEMSLLLDRDRKRAAQHIAPQAQSPYPERLQAIAREAGLWLTVGAPVRVE
DDRYANRQWLFYPRGESPVHYDKIHMFDVDLADGESWRESNAYRPGEEVVLVDDTPVGRLGLTICYDLRFPALFEELGNA
RADCIAIPAAFTRPTGAAHWHTLVRARAIEASAFVVAAAQVGEHEDGRKTYGHSLVVDPWGEVLLDMSGDKPGLGFADID
LARIEEVRRQVPSLANRRPIPQSTAQ

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI297632350, Length=265, Percent_Identity=39.2452830188679, Blast_Score=181, Evalue=7e-46,
Organism=Homo sapiens, GI297632348, Length=265, Percent_Identity=39.2452830188679, Blast_Score=181, Evalue=8e-46,
Organism=Homo sapiens, GI5031947, Length=265, Percent_Identity=39.2452830188679, Blast_Score=181, Evalue=9e-46,
Organism=Homo sapiens, GI9910460, Length=245, Percent_Identity=31.8367346938775, Blast_Score=135, Evalue=3e-32,
Organism=Homo sapiens, GI297632346, Length=186, Percent_Identity=33.8709677419355, Blast_Score=99, Evalue=4e-21,
Organism=Escherichia coli, GI1786412, Length=169, Percent_Identity=33.1360946745562, Blast_Score=64, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17556280, Length=264, Percent_Identity=37.8787878787879, Blast_Score=177, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6322335, Length=290, Percent_Identity=31.0344827586207, Blast_Score=145, Evalue=9e-36,
Organism=Saccharomyces cerevisiae, GI6323383, Length=219, Percent_Identity=31.5068493150685, Blast_Score=122, Evalue=7e-29,
Organism=Drosophila melanogaster, GI17933642, Length=262, Percent_Identity=36.6412213740458, Blast_Score=168, Evalue=3e-42,
Organism=Drosophila melanogaster, GI21355835, Length=202, Percent_Identity=32.1782178217822, Blast_Score=118, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 29470; Mature: 29339

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSGIDPEANFAAIEQAAREASAGGAEMLFTPEMSLLLDRDRKRAAQHIAPQAQSPYPER
CCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCHHHHHCCHHHHHHHHCCCCCCCCCHHH
LQAIAREAGLWLTVGAPVRVEDDRYANRQWLFYPRGESPVHYDKIHMFDVDLADGESWRE
HHHHHHHCCEEEEECCCEEECCCCCCCCEEEEEECCCCCCEECEEEEEEEECCCCCCHHH
SNAYRPGEEVVLVDDTPVGRLGLTICYDLRFPALFEELGNARADCIAIPAAFTRPTGAAH
CCCCCCCCCEEEECCCCCCCCCEEEEEECCCHHHHHHHCCCCCCEEEECCHHCCCCCCHH
WHTLVRARAIEASAFVVAAAQVGEHEDGRKTYGHSLVVDPWGEVLLDMSGDKPGLGFADI
HHHHHHHHHHHHHHHEEEHHHHCCCCCCCHHHCCEEEECCHHHEEEECCCCCCCCCCCCC
DLARIEEVRRQVPSLANRRPIPQSTAQ
HHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TSGIDPEANFAAIEQAAREASAGGAEMLFTPEMSLLLDRDRKRAAQHIAPQAQSPYPER
CCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCHHHHHCCHHHHHHHHCCCCCCCCCHHH
LQAIAREAGLWLTVGAPVRVEDDRYANRQWLFYPRGESPVHYDKIHMFDVDLADGESWRE
HHHHHHHCCEEEEECCCEEECCCCCCCCEEEEEECCCCCCEECEEEEEEEECCCCCCHHH
SNAYRPGEEVVLVDDTPVGRLGLTICYDLRFPALFEELGNARADCIAIPAAFTRPTGAAH
CCCCCCCCCEEEECCCCCCCCCEEEEEECCCHHHHHHHCCCCCCEEEECCHHCCCCCCHH
WHTLVRARAIEASAFVVAAAQVGEHEDGRKTYGHSLVVDPWGEVLLDMSGDKPGLGFADI
HHHHHHHHHHHHHHHEEEHHHHCCCCCCCHHHCCEEEECCHHHEEEECCCCCCCCCCCCC
DLARIEEVRRQVPSLANRRPIPQSTAQ
HHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]