Click here to switch to the map view.

The map label for this gene is pdhA [H]

Identifier: 85374108

GI number: 85374108

Start: 1331286

End: 1332383

Strand: Reverse

Name: pdhA [H]

Synonym: ELI_06405

Alternate gene names: 85374108

Gene position: 1332383-1331286 (Counterclockwise)

Preceding gene: 85374109

Following gene: 85374107

Centisome position: 43.65

GC content: 60.66

Gene sequence:

>1098_bases
TTGGCCAAAGCCAGCACGCGAAAGACGCCCGCGAAGAAGAAATCGTCCGTCAAGCCCAATCCAGCAGCGACCGACGAGGA
TTTCGTCCTGCACAGCCTCCAGGAGGAGTTCGAGAAGAACAAGCGCTACAAGGCGAGCGACAAGGAGATGCTCGATTTCT
ACGAGCAGATGCTCCTCATCCGCCGGTTCGAGGAGCGGGCGGGGCAGCTCTACGGTCTCGGCCTGATCGGCGGTTTCTGT
CATCTTTATATCGGTCAGGAAGCTGTAGCGATCGGCCTTCAGAGTGCGCTCGACGGGGACAAGGACAGCGTCATCACCGG
CTATCGCGACCATGGCCACATGCTCGCCTATGGCATCGATCCCAAAGTTATCATGGCCGAGCTGACCGGCCGCGAAGCCG
GTATCTCCAAGGGCAAGGGCGGCTCGATGCACATGTTCAGCACCGAGCATAAATTCTACGGCGGCCACGGAATTGTCGGC
GCGCAGGTGGCGCTGGGCGGCGGGCTCGCCTTTGCGCACAAATACAATGAAGACGGCGGCATTTGCCTGGCTTATTTCGG
CGATGGGGCGGCCAACCAGGGGCAGGTGTACGAGACCTTCAACATGGCGGCGCTCTGGAACCTGCCGATCGTCTTCGTGG
TCGAGGACAACCAGTATGCGATGGGTACGTCGACCAAGCGTTCCAGTGCGGAGACGCGCTTCTACCGGCGCGGCACATCG
TTCCGCATTCCGGGGATGGAAGTGAACGGGATGGATGTGCTGGAAGTACGCGCCGCGGCCGAGATCGCATTCAAGCATGT
GCGCGAAGGCAACGGCCCGGTGCTGATGGAATGCAATACCTATCGCTATCGCGGGCACTCGATGTCCGACCCGGCCAAGT
ACCGCACGCGCGAAGAAGTGCAGGACCAACGCGATCATCACGATCCGATAGAAGGCCTGAAGAAAGCGCTGATCGAGCAG
GGCAAGAGCGAGGACGACCTGAAAGCCATCGACAAGGCGATCCGCGCGCAGGTTTCCGAAGCGGCCGACTTCGCCGAGAA
CTCGCCCGAGCCCGATCCGAGCGAACTCTACACCGATGTCCTGGTGGAGGAGTATTGA

Upstream 100 bases:

>100_bases
CGGACGCGCACAGGCGTTGCGCGGCGGGCGCGCTTGCCCTTATAGGCTGGCGCGGCCGCTGCTTCCTTGAGGACGGCCTA
ACTTTTGGAGGAATGCCCCC

Downstream 100 bases:

>100_bases
GCCATGGCGATTGAACTCAAGATGCCGGCGCTCTCGCCGACGATGGAAGAGGGCACGCTCGCCAAGTGGCTGAAGGCCGA
AGGCGACGAGATCGTCGCGG

Product: pyruvate dehydrogenase E1 component alpha subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MAKASTRKTPAKKKSSVKPNPAATDEDFVLHSLQEEFEKNKRYKASDKEMLDFYEQMLLIRRFEERAGQLYGLGLIGGFC
HLYIGQEAVAIGLQSALDGDKDSVITGYRDHGHMLAYGIDPKVIMAELTGREAGISKGKGGSMHMFSTEHKFYGGHGIVG
AQVALGGGLAFAHKYNEDGGICLAYFGDGAANQGQVYETFNMAALWNLPIVFVVEDNQYAMGTSTKRSSAETRFYRRGTS
FRIPGMEVNGMDVLEVRAAAEIAFKHVREGNGPVLMECNTYRYRGHSMSDPAKYRTREEVQDQRDHHDPIEGLKKALIEQ
GKSEDDLKAIDKAIRAQVSEAADFAENSPEPDPSELYTDVLVEEY

Sequences:

>Translated_365_residues
MAKASTRKTPAKKKSSVKPNPAATDEDFVLHSLQEEFEKNKRYKASDKEMLDFYEQMLLIRRFEERAGQLYGLGLIGGFC
HLYIGQEAVAIGLQSALDGDKDSVITGYRDHGHMLAYGIDPKVIMAELTGREAGISKGKGGSMHMFSTEHKFYGGHGIVG
AQVALGGGLAFAHKYNEDGGICLAYFGDGAANQGQVYETFNMAALWNLPIVFVVEDNQYAMGTSTKRSSAETRFYRRGTS
FRIPGMEVNGMDVLEVRAAAEIAFKHVREGNGPVLMECNTYRYRGHSMSDPAKYRTREEVQDQRDHHDPIEGLKKALIEQ
GKSEDDLKAIDKAIRAQVSEAADFAENSPEPDPSELYTDVLVEEY
>Mature_364_residues
AKASTRKTPAKKKSSVKPNPAATDEDFVLHSLQEEFEKNKRYKASDKEMLDFYEQMLLIRRFEERAGQLYGLGLIGGFCH
LYIGQEAVAIGLQSALDGDKDSVITGYRDHGHMLAYGIDPKVIMAELTGREAGISKGKGGSMHMFSTEHKFYGGHGIVGA
QVALGGGLAFAHKYNEDGGICLAYFGDGAANQGQVYETFNMAALWNLPIVFVVEDNQYAMGTSTKRSSAETRFYRRGTSF
RIPGMEVNGMDVLEVRAAAEIAFKHVREGNGPVLMECNTYRYRGHSMSDPAKYRTREEVQDQRDHHDPIEGLKKALIEQG
KSEDDLKAIDKAIRAQVSEAADFAENSPEPDPSELYTDVLVEEY

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4885543, Length=310, Percent_Identity=51.6129032258064, Blast_Score=314, Evalue=8e-86,
Organism=Homo sapiens, GI291084742, Length=308, Percent_Identity=51.2987012987013, Blast_Score=298, Evalue=5e-81,
Organism=Homo sapiens, GI4505685, Length=308, Percent_Identity=51.2987012987013, Blast_Score=298, Evalue=5e-81,
Organism=Homo sapiens, GI291084744, Length=315, Percent_Identity=50.1587301587302, Blast_Score=291, Evalue=6e-79,
Organism=Homo sapiens, GI291084757, Length=308, Percent_Identity=45.4545454545455, Blast_Score=246, Evalue=3e-65,
Organism=Homo sapiens, GI11386135, Length=320, Percent_Identity=31.5625, Blast_Score=131, Evalue=9e-31,
Organism=Homo sapiens, GI258645172, Length=325, Percent_Identity=31.6923076923077, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI32564172, Length=323, Percent_Identity=49.2260061919505, Blast_Score=311, Evalue=3e-85,
Organism=Caenorhabditis elegans, GI17536047, Length=323, Percent_Identity=49.2260061919505, Blast_Score=311, Evalue=4e-85,
Organism=Caenorhabditis elegans, GI86563357, Length=289, Percent_Identity=30.7958477508651, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI86563355, Length=289, Percent_Identity=30.7958477508651, Blast_Score=115, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6321026, Length=325, Percent_Identity=49.8461538461538, Blast_Score=319, Evalue=5e-88,
Organism=Drosophila melanogaster, GI24639744, Length=339, Percent_Identity=50.7374631268437, Blast_Score=337, Evalue=9e-93,
Organism=Drosophila melanogaster, GI28571106, Length=339, Percent_Identity=50.7374631268437, Blast_Score=337, Evalue=9e-93,
Organism=Drosophila melanogaster, GI24639740, Length=339, Percent_Identity=50.7374631268437, Blast_Score=336, Evalue=1e-92,
Organism=Drosophila melanogaster, GI24639746, Length=306, Percent_Identity=53.2679738562092, Blast_Score=325, Evalue=3e-89,
Organism=Drosophila melanogaster, GI24639748, Length=317, Percent_Identity=50.788643533123, Blast_Score=302, Evalue=2e-82,
Organism=Drosophila melanogaster, GI21355903, Length=284, Percent_Identity=27.8169014084507, Blast_Score=102, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017
- InterPro:   IPR017597 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 40474; Mature: 40343

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKASTRKTPAKKKSSVKPNPAATDEDFVLHSLQEEFEKNKRYKASDKEMLDFYEQMLLI
CCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
RRFEERAGQLYGLGLIGGFCHLYIGQEAVAIGLQSALDGDKDSVITGYRDHGHMLAYGID
HHHHHHCCCEEEHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEECCCCCCCEEEECCC
PKVIMAELTGREAGISKGKGGSMHMFSTEHKFYGGHGIVGAQVALGGGLAFAHKYNEDGG
CCEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEEEECCCEEEEEEECCCCC
ICLAYFGDGAANQGQVYETFNMAALWNLPIVFVVEDNQYAMGTSTKRSSAETRFYRRGTS
EEEEEECCCCCCCCCEEEHCCEEEEECCCEEEEEECCCEEECCCCCCCHHHHHHHHCCCC
FRIPGMEVNGMDVLEVRAAAEIAFKHVREGNGPVLMECNTYRYRGHSMSDPAKYRTREEV
EECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCEEECCCCCCCCHHHHHHHHH
QDQRDHHDPIEGLKKALIEQGKSEDDLKAIDKAIRAQVSEAADFAENSPEPDPSELYTDV
HHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
LVEEY
HHHCC
>Mature Secondary Structure 
AKASTRKTPAKKKSSVKPNPAATDEDFVLHSLQEEFEKNKRYKASDKEMLDFYEQMLLI
CCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
RRFEERAGQLYGLGLIGGFCHLYIGQEAVAIGLQSALDGDKDSVITGYRDHGHMLAYGID
HHHHHHCCCEEEHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEECCCCCCCEEEECCC
PKVIMAELTGREAGISKGKGGSMHMFSTEHKFYGGHGIVGAQVALGGGLAFAHKYNEDGG
CCEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEEEECCCEEEEEEECCCCC
ICLAYFGDGAANQGQVYETFNMAALWNLPIVFVVEDNQYAMGTSTKRSSAETRFYRRGTS
EEEEEECCCCCCCCCEEEHCCEEEEECCCEEEEEECCCEEECCCCCCCHHHHHHHHCCCC
FRIPGMEVNGMDVLEVRAAAEIAFKHVREGNGPVLMECNTYRYRGHSMSDPAKYRTREEV
EECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCEEECCCCCCCCHHHHHHHHH
QDQRDHHDPIEGLKKALIEQGKSEDDLKAIDKAIRAQVSEAADFAENSPEPDPSELYTDV
HHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
LVEEY
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]