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The map label for this gene is pdhB [H]

Identifier: 85374107

GI number: 85374107

Start: 1329894

End: 1331282

Strand: Reverse

Name: pdhB [H]

Synonym: ELI_06400

Alternate gene names: 85374107

Gene position: 1331282-1329894 (Counterclockwise)

Preceding gene: 85374108

Following gene: 85374100

Centisome position: 43.61

GC content: 62.85

Gene sequence:

>1389_bases
ATGGCGATTGAACTCAAGATGCCGGCGCTCTCGCCGACGATGGAAGAGGGCACGCTCGCCAAGTGGCTGAAGGCCGAAGG
CGACGAGATCGTCGCGGGCGACATCATTGCCGAGATCGAGACCGACAAGGCGACGATGGAATTCGAAGCGGTCGACGAAG
GCACGCTCGGCAAGATTCTCGTCGAAGAGGGTACCGAGAACGTGAAGGTCGGCACGGTGATCGCCATGCTCGCCGCTGAC
GGTGAAGATGTTTCCGACGTCGAAGCGCCAGCCGAGAGCGCGCCGGTCGACGATGTTCCGGGCGAGGGCAAGGATGTCGG
CCAGGACGACGCCGATGGCTCGATCACGCCGGACAAGCCTAAGCGCGAGCCCAAGGCCGATCCGGAAATCCCGGAAGGCA
CCAACATGGTCTCCGTGTCGGTCCGCGAAGCCCTGCGCGATGCGATGGCCGAGGAAATGCGCCGCGACGAACGTGTGTTC
GTGATGGGCGAGGAAGTCGCCGAGTATCAGGGCGCCTACAAGGTGACCCAGGGGCTTCTCGACGAGTTCGGACCCAAGCG
CGTGATCGATACGCCGATCACCGAATACGGCTTCGCTGGCATCGGCACCGGCGCGGCGATGGGCGGCCTGCGCCCGATCG
TCGAATTCATGACCTTCAACTTCGCGATGCAGGCGATCGACCATATCGTGAACTCTGCCGCAAAGACTAACTACATGTCC
GGCGGCCAGATGCGCTGCCCGGTCGTGTTTCGCGGGCCCAATGGAGCGGCCAGCCGCGTCGGCGCGCAGCACAGCCAGAA
TTATGGCCCATGGTACGCCAGCGTGCCGGGCCTGATCGTCATCGCGCCCTATGATTCATCGGATGCCAAGGGCCTGATGA
AGGCCGCGATCCGCTGCGAAGATCCGGTGGTGTTTCTCGAGAACGAGCTGGTCTACGGACGCAGTTTCGAACTGCCCGAA
CTCGACGATCACGTCCTGCCGATCGGCAAGGCGCGGATCATGCGCGAAGGATTGGACGTCACCATCGTCGCCTATTCGAT
CGCCGTCGGCTTCGCGCTGGAAGCGGCTGAGCAACTGGCGGAAGAAGGCATCGACGCCGAAGTCATCGACTTGCGCACGC
TGCGTCCGCTCGACAAGGAAGCGATTCTGACCTCGCTCGCCAAGACCAACCGGCTGATCATCGCCGAAGAAGGCTGGCCG
ACCTGCTCGATCGCTTCGGAGATCGCAGCGATCTGCATGGAAGAAGGGTTCGACCATCTCGATGCGCCGGTGCTGCGGGT
AACGGACGAGGACGTGCCGTTGCCCTATGCTGCCAACCTGGAAAAGCTGGCGTTGATCGACGCGCCGCGGATCGTGAAGG
CGGCGAAGAAGGTCTGTTACGTCGACTGA

Upstream 100 bases:

>100_bases
CCGCGCGCAGGTTTCCGAAGCGGCCGACTTCGCCGAGAACTCGCCCGAGCCCGATCCGAGCGAACTCTACACCGATGTCC
TGGTGGAGGAGTATTGAGCC

Downstream 100 bases:

>100_bases
CGATGCGAATTCGGGCTAGCCGGGCGTCGCGCTGTACACATCGCAGCGCGACACCGCAGGCGTGCCGAAATCGGTGCGCG
TGTAGATTTCCGTCCGATCA

Product: pyruvate dehydrogenase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MAIELKMPALSPTMEEGTLAKWLKAEGDEIVAGDIIAEIETDKATMEFEAVDEGTLGKILVEEGTENVKVGTVIAMLAAD
GEDVSDVEAPAESAPVDDVPGEGKDVGQDDADGSITPDKPKREPKADPEIPEGTNMVSVSVREALRDAMAEEMRRDERVF
VMGEEVAEYQGAYKVTQGLLDEFGPKRVIDTPITEYGFAGIGTGAAMGGLRPIVEFMTFNFAMQAIDHIVNSAAKTNYMS
GGQMRCPVVFRGPNGAASRVGAQHSQNYGPWYASVPGLIVIAPYDSSDAKGLMKAAIRCEDPVVFLENELVYGRSFELPE
LDDHVLPIGKARIMREGLDVTIVAYSIAVGFALEAAEQLAEEGIDAEVIDLRTLRPLDKEAILTSLAKTNRLIIAEEGWP
TCSIASEIAAICMEEGFDHLDAPVLRVTDEDVPLPYAANLEKLALIDAPRIVKAAKKVCYVD

Sequences:

>Translated_462_residues
MAIELKMPALSPTMEEGTLAKWLKAEGDEIVAGDIIAEIETDKATMEFEAVDEGTLGKILVEEGTENVKVGTVIAMLAAD
GEDVSDVEAPAESAPVDDVPGEGKDVGQDDADGSITPDKPKREPKADPEIPEGTNMVSVSVREALRDAMAEEMRRDERVF
VMGEEVAEYQGAYKVTQGLLDEFGPKRVIDTPITEYGFAGIGTGAAMGGLRPIVEFMTFNFAMQAIDHIVNSAAKTNYMS
GGQMRCPVVFRGPNGAASRVGAQHSQNYGPWYASVPGLIVIAPYDSSDAKGLMKAAIRCEDPVVFLENELVYGRSFELPE
LDDHVLPIGKARIMREGLDVTIVAYSIAVGFALEAAEQLAEEGIDAEVIDLRTLRPLDKEAILTSLAKTNRLIIAEEGWP
TCSIASEIAAICMEEGFDHLDAPVLRVTDEDVPLPYAANLEKLALIDAPRIVKAAKKVCYVD
>Mature_461_residues
AIELKMPALSPTMEEGTLAKWLKAEGDEIVAGDIIAEIETDKATMEFEAVDEGTLGKILVEEGTENVKVGTVIAMLAADG
EDVSDVEAPAESAPVDDVPGEGKDVGQDDADGSITPDKPKREPKADPEIPEGTNMVSVSVREALRDAMAEEMRRDERVFV
MGEEVAEYQGAYKVTQGLLDEFGPKRVIDTPITEYGFAGIGTGAAMGGLRPIVEFMTFNFAMQAIDHIVNSAAKTNYMSG
GQMRCPVVFRGPNGAASRVGAQHSQNYGPWYASVPGLIVIAPYDSSDAKGLMKAAIRCEDPVVFLENELVYGRSFELPEL
DDHVLPIGKARIMREGLDVTIVAYSIAVGFALEAAEQLAEEGIDAEVIDLRTLRPLDKEAILTSLAKTNRLIIAEEGWPT
CSIASEIAAICMEEGFDHLDAPVLRVTDEDVPLPYAANLEKLALIDAPRIVKAAKKVCYVD

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156564403, Length=325, Percent_Identity=55.6923076923077, Blast_Score=372, Evalue=1e-103,
Organism=Homo sapiens, GI291084858, Length=325, Percent_Identity=52.6153846153846, Blast_Score=340, Evalue=1e-93,
Organism=Homo sapiens, GI4557353, Length=336, Percent_Identity=33.6309523809524, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI34101272, Length=336, Percent_Identity=33.6309523809524, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI203098753, Length=93, Percent_Identity=50.5376344086022, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI203098816, Length=93, Percent_Identity=50.5376344086022, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI31711992, Length=81, Percent_Identity=50.6172839506173, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI260898739, Length=58, Percent_Identity=56.8965517241379, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1786622, Length=319, Percent_Identity=22.884012539185, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17538422, Length=324, Percent_Identity=61.1111111111111, Blast_Score=397, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17506935, Length=329, Percent_Identity=34.3465045592705, Blast_Score=165, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI17560088, Length=79, Percent_Identity=50.6329113924051, Blast_Score=89, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6319698, Length=330, Percent_Identity=60.3030303030303, Blast_Score=408, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6324258, Length=78, Percent_Identity=56.4102564102564, Blast_Score=90, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6321632, Length=89, Percent_Identity=43.8202247191011, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI21358145, Length=324, Percent_Identity=58.0246913580247, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24650940, Length=324, Percent_Identity=58.0246913580247, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI160714832, Length=322, Percent_Identity=35.0931677018634, Blast_Score=181, Evalue=1e-45,
Organism=Drosophila melanogaster, GI160714828, Length=322, Percent_Identity=35.0931677018634, Blast_Score=180, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24650943, Length=91, Percent_Identity=65.9340659340659, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24650945, Length=91, Percent_Identity=65.9340659340659, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI20129315, Length=83, Percent_Identity=49.3975903614458, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24582497, Length=67, Percent_Identity=49.2537313432836, Blast_Score=72, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR011053
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 49750; Mature: 49619

Theoretical pI: Translated: 4.11; Mature: 4.11

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIELKMPALSPTMEEGTLAKWLKAEGDEIVAGDIIAEIETDKATMEFEAVDEGTLGKIL
CEEEEECCCCCCCCCCCHHHHHHHCCCCEEEECCEEEEEECCCCEEEEEECCCCCCCEEE
VEEGTENVKVGTVIAMLAADGEDVSDVEAPAESAPVDDVPGEGKDVGQDDADGSITPDKP
EECCCCCEEHHHEEEEEECCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
KREPKADPEIPEGTNMVSVSVREALRDAMAEEMRRDERVFVMGEEVAEYQGAYKVTQGLL
CCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHCCHHHHHHHHH
DEFGPKRVIDTPITEYGFAGIGTGAAMGGLRPIVEFMTFNFAMQAIDHIVNSAAKTNYMS
HHCCCCCEECCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GGQMRCPVVFRGPNGAASRVGAQHSQNYGPWYASVPGLIVIAPYDSSDAKGLMKAAIRCE
CCCEECCEEEECCCCHHHHHCCCCCCCCCCEEECCCCEEEEEECCCCCHHHHHHHHHHCC
DPVVFLENELVYGRSFELPELDDHVLPIGKARIMREGLDVTIVAYSIAVGFALEAAEQLA
CCEEEEECCEEECCCCCCCCCCCCCCCCHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHH
EEGIDAEVIDLRTLRPLDKEAILTSLAKTNRLIIAEEGWPTCSIASEIAAICMEEGFDHL
HCCCCEEEEEHHHCCCCCHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCC
DAPVLRVTDEDVPLPYAANLEKLALIDAPRIVKAAKKVCYVD
CCCEEEECCCCCCCCCCCCCCCEEEECCHHHHHHHHHHCCCC
>Mature Secondary Structure 
AIELKMPALSPTMEEGTLAKWLKAEGDEIVAGDIIAEIETDKATMEFEAVDEGTLGKIL
EEEEECCCCCCCCCCCHHHHHHHCCCCEEEECCEEEEEECCCCEEEEEECCCCCCCEEE
VEEGTENVKVGTVIAMLAADGEDVSDVEAPAESAPVDDVPGEGKDVGQDDADGSITPDKP
EECCCCCEEHHHEEEEEECCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
KREPKADPEIPEGTNMVSVSVREALRDAMAEEMRRDERVFVMGEEVAEYQGAYKVTQGLL
CCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHCCHHHHHHHHH
DEFGPKRVIDTPITEYGFAGIGTGAAMGGLRPIVEFMTFNFAMQAIDHIVNSAAKTNYMS
HHCCCCCEECCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GGQMRCPVVFRGPNGAASRVGAQHSQNYGPWYASVPGLIVIAPYDSSDAKGLMKAAIRCE
CCCEECCEEEECCCCHHHHHCCCCCCCCCCEEECCCCEEEEEECCCCCHHHHHHHHHHCC
DPVVFLENELVYGRSFELPELDDHVLPIGKARIMREGLDVTIVAYSIAVGFALEAAEQLA
CCEEEEECCEEECCCCCCCCCCCCCCCCHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHH
EEGIDAEVIDLRTLRPLDKEAILTSLAKTNRLIIAEEGWPTCSIASEIAAICMEEGFDHL
HCCCCEEEEEHHHCCCCCHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCC
DAPVLRVTDEDVPLPYAANLEKLALIDAPRIVKAAKKVCYVD
CCCEEEECCCCCCCCCCCCCCCEEEECCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]