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The map label for this gene is mrdA [H]

Identifier: 85373982

GI number: 85373982

Start: 1192062

End: 1194107

Strand: Reverse

Name: mrdA [H]

Synonym: ELI_05775

Alternate gene names: 85373982

Gene position: 1194107-1192062 (Counterclockwise)

Preceding gene: 85373983

Following gene: 85373981

Centisome position: 39.12

GC content: 63.98

Gene sequence:

>2046_bases
ATGGCTCTCGGCTCGTCGCACCTTCCCGTCAGTTCCAATACCCTGAAAGATCGCTTCGACCGCCGCAGTTTCACGCTCGG
CACACTCGGTCTCGGCATCGGCACGCTGCTCGCGGTGCGGATGGGCTATATCGCGATCGCGGAAAATGAACGTTACGCGG
TCGAAAGCGAGAGCAACCGCGTCAACCTGACGCTGATCCCGCCGCGGCGCGGCTGGCTGCTCGACCGCAACGGCGCCCCC
CTCGCCTCCAACCGCGCCGACTTTCGTGTCGATATCATCCCCGAGCGGATGACCGACAAGATGGAGACCTTGAACGAACT
GGGTGAGTTGCTCGATCTCGACGCAAACCGCATGCGCGACCTGCGCAAGGAAATCGAGGAATCGCGCGGCTATGCACCGA
TCGAGATTGCCAGCGGCCTCGACTATGAGCAGTTCGCTGCCGTCTCGCTGCGCGTCCCCGACATGCCCGGCGTCGTTCCG
CAGCGGGGTTTTTCTCGCTTCTATCCCACCGGCCCCTCGGTGGGACACCTGCTCGGCTATGTCGGCCCCGCGTCGGCGGA
GGAGTATGAAGAGGATCGCAATCCGCTGCTGATTACGCCCGGCTACAAGATCGGCAAGGACGGGCTGGAAAAGCAATTCG
AGCAAACCCTGCGCGGCGAGCCCGGGGCGCGCCGGGTCGAGGTAACCGCCTCGGGCCGTATCGTGCGAGACCTCAGCACC
CGGCCGGATGTGCAAGGCAATCCCGTCAAGCTGACCATCGACGGTCCGCTTCAGGACTATGCCGCGCGCCGGATCGGTCT
CGAAAGCGGTTCGGTCGTCGTGATGGATTGCGACACGGGCGACCTGCTCTGCATGGCTTCGATGCCGAGTTTCGACCCCA
ACAGTTTCTCCGAAGGCATCGGCAGCGTCGAATACGCCATGCTGCGCGAGGACGAGCGCGTGCCGCTCCGCAACAAGGTG
CTCAAGGGCCTTTATCCGCCCGGATCGACGATCAAGCCGATGCACTGCATGGAGTTCCTGCAGGAAGGCATCAGCCCCGA
TGAGACCATCGTGTGCGGCGGCGGCCGGCGGATCGGCAATCGCTTCTTCAACTGCTGGAGCAACCACGGCGTCGTCGACA
TGGCCAAGGCCATCTACCAGAGCTGCGACAGTTATTTCTATCATTTCGCCCAGCAGGTCGGTTTTGAGAAAATCGCCGCC
TTCGCCAAGAAGATGGGGCTGGGCCAGGAATTTCCTTTACCCGTGACGAGCCAGTTCTACGGCACCGTTCCGAGCCCCGC
ATGGAAGGAAGAGAAGTACGGCGATCCCTGGCGCGCCTTCGATACAGTCAATGCCAGTATCGGTCAGGGGTATTATCTCG
CCAGCCCGCTGCAATTGGCGGTCATGTCTGCGCGTCTCGCAACCGGCAAGGCACTGATGCCGCGCCTGACCCTCGACGGC
AAAACACCCCAACCCGCCAGTCTCGGCTTTTCGCAGGATCATATCGCACATGTCCGGCAGGCGATGAGCGACGTCGTCAA
CGGTCCCGGTACGGCCGGACGTGCGCGGCTGCCGCTCGAAGAGGTCAAAATGGCGGGCAAGACAGGGACGGCGCAGGTGG
TCAGCCTGAGCATATCCGACGGCCGTTCGGGCCCGTGGAAATATCGCGACCATGGCCTGTTCGTATTCTTCGCGCCGCTC
GACAAGCCGAAATATGCTGGCGCCGTGGTGATCGAGCACGGCGGTGGATCGGGCGCCGCCTACCCCATCGCGCGTGACGT
GATGACGTTCCTGTTCGACCCGGCGAAGGGTCTTGAGGCGCTGAATGCGCTGGAAGAGCAATGGGGCGGCACTGCCCAGC
AGCGCCTGGTTCGCAAATATGCCGCCTATGCCGCCGAACGCGGTGCCGAGGTCGAGCCGGCGCCCCGGCGCGCCGAAGAG
ATCTTCGACCGGGTCGACGCCGAAGCGCGCATGGCCGCGCAACAGGCGGAGGCTCTGGGGCAAGAAGCCAGCATCAGTCG
CAACGAGAGCGCGCGCGGCACCGAGCCGGCCGGGGGGAACCGGTGA

Upstream 100 bases:

>100_bases
TGCCGCAGGCCTTGCTGTCGATTTTCGTATTCCCCATTATCGCTGTCATGGTGGCAGGTCTCGACAGGCTCCGGCTCTTG
CGCGTGAGGGTTATCGGCTG

Downstream 100 bases:

>100_bases
ATTCGGTCATCCCCGCGCCTATCGCGCGCCAGCCTTGGGGCATGCTTATCCCGCTGATATTGCTGACCTCCTTCGGTGGG
GCAGTGCTTTTCTCGGCTGC

Product: cell division protein

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 681; Mature: 680

Protein sequence:

>681_residues
MALGSSHLPVSSNTLKDRFDRRSFTLGTLGLGIGTLLAVRMGYIAIAENERYAVESESNRVNLTLIPPRRGWLLDRNGAP
LASNRADFRVDIIPERMTDKMETLNELGELLDLDANRMRDLRKEIEESRGYAPIEIASGLDYEQFAAVSLRVPDMPGVVP
QRGFSRFYPTGPSVGHLLGYVGPASAEEYEEDRNPLLITPGYKIGKDGLEKQFEQTLRGEPGARRVEVTASGRIVRDLST
RPDVQGNPVKLTIDGPLQDYAARRIGLESGSVVVMDCDTGDLLCMASMPSFDPNSFSEGIGSVEYAMLREDERVPLRNKV
LKGLYPPGSTIKPMHCMEFLQEGISPDETIVCGGGRRIGNRFFNCWSNHGVVDMAKAIYQSCDSYFYHFAQQVGFEKIAA
FAKKMGLGQEFPLPVTSQFYGTVPSPAWKEEKYGDPWRAFDTVNASIGQGYYLASPLQLAVMSARLATGKALMPRLTLDG
KTPQPASLGFSQDHIAHVRQAMSDVVNGPGTAGRARLPLEEVKMAGKTGTAQVVSLSISDGRSGPWKYRDHGLFVFFAPL
DKPKYAGAVVIEHGGGSGAAYPIARDVMTFLFDPAKGLEALNALEEQWGGTAQQRLVRKYAAYAAERGAEVEPAPRRAEE
IFDRVDAEARMAAQQAEALGQEASISRNESARGTEPAGGNR

Sequences:

>Translated_681_residues
MALGSSHLPVSSNTLKDRFDRRSFTLGTLGLGIGTLLAVRMGYIAIAENERYAVESESNRVNLTLIPPRRGWLLDRNGAP
LASNRADFRVDIIPERMTDKMETLNELGELLDLDANRMRDLRKEIEESRGYAPIEIASGLDYEQFAAVSLRVPDMPGVVP
QRGFSRFYPTGPSVGHLLGYVGPASAEEYEEDRNPLLITPGYKIGKDGLEKQFEQTLRGEPGARRVEVTASGRIVRDLST
RPDVQGNPVKLTIDGPLQDYAARRIGLESGSVVVMDCDTGDLLCMASMPSFDPNSFSEGIGSVEYAMLREDERVPLRNKV
LKGLYPPGSTIKPMHCMEFLQEGISPDETIVCGGGRRIGNRFFNCWSNHGVVDMAKAIYQSCDSYFYHFAQQVGFEKIAA
FAKKMGLGQEFPLPVTSQFYGTVPSPAWKEEKYGDPWRAFDTVNASIGQGYYLASPLQLAVMSARLATGKALMPRLTLDG
KTPQPASLGFSQDHIAHVRQAMSDVVNGPGTAGRARLPLEEVKMAGKTGTAQVVSLSISDGRSGPWKYRDHGLFVFFAPL
DKPKYAGAVVIEHGGGSGAAYPIARDVMTFLFDPAKGLEALNALEEQWGGTAQQRLVRKYAAYAAERGAEVEPAPRRAEE
IFDRVDAEARMAAQQAEALGQEASISRNESARGTEPAGGNR
>Mature_680_residues
ALGSSHLPVSSNTLKDRFDRRSFTLGTLGLGIGTLLAVRMGYIAIAENERYAVESESNRVNLTLIPPRRGWLLDRNGAPL
ASNRADFRVDIIPERMTDKMETLNELGELLDLDANRMRDLRKEIEESRGYAPIEIASGLDYEQFAAVSLRVPDMPGVVPQ
RGFSRFYPTGPSVGHLLGYVGPASAEEYEEDRNPLLITPGYKIGKDGLEKQFEQTLRGEPGARRVEVTASGRIVRDLSTR
PDVQGNPVKLTIDGPLQDYAARRIGLESGSVVVMDCDTGDLLCMASMPSFDPNSFSEGIGSVEYAMLREDERVPLRNKVL
KGLYPPGSTIKPMHCMEFLQEGISPDETIVCGGGRRIGNRFFNCWSNHGVVDMAKAIYQSCDSYFYHFAQQVGFEKIAAF
AKKMGLGQEFPLPVTSQFYGTVPSPAWKEEKYGDPWRAFDTVNASIGQGYYLASPLQLAVMSARLATGKALMPRLTLDGK
TPQPASLGFSQDHIAHVRQAMSDVVNGPGTAGRARLPLEEVKMAGKTGTAQVVSLSISDGRSGPWKYRDHGLFVFFAPLD
KPKYAGAVVIEHGGGSGAAYPIARDVMTFLFDPAKGLEALNALEEQWGGTAQQRLVRKYAAYAAERGAEVEPAPRRAEEI
FDRVDAEARMAAQQAEALGQEASISRNESARGTEPAGGNR

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=598, Percent_Identity=30.4347826086957, Blast_Score=287, Evalue=2e-78,
Organism=Escherichia coli, GI1786272, Length=563, Percent_Identity=24.1563055062167, Blast_Score=127, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 74430; Mature: 74299

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALGSSHLPVSSNTLKDRFDRRSFTLGTLGLGIGTLLAVRMGYIAIAENERYAVESESNR
CCCCCCCCCCCCCHHHHHHCCCCEEEHHHHHHHHHHHHHHHCEEEEECCCEEEEECCCCE
VNLTLIPPRRGWLLDRNGAPLASNRADFRVDIIPERMTDKMETLNELGELLDLDANRMRD
EEEEEECCCCCEEECCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCHHHHHH
LRKEIEESRGYAPIEIASGLDYEQFAAVSLRVPDMPGVVPQRGFSRFYPTGPSVGHLLGY
HHHHHHHHCCCCCEEECCCCCHHHHEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHC
VGPASAEEYEEDRNPLLITPGYKIGKDGLEKQFEQTLRGEPGARRVEVTASGRIVRDLST
CCCCCHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCEEECCCC
RPDVQGNPVKLTIDGPLQDYAARRIGLESGSVVVMDCDTGDLLCMASMPSFDPNSFSEGI
CCCCCCCCEEEEECCCHHHHHHHHCCCCCCCEEEEECCCCCEEEEECCCCCCCCCHHHHC
GSVEYAMLREDERVPLRNKVLKGLYPPGSTIKPMHCMEFLQEGISPDETIVCGGGRRIGN
CCHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCHHHHH
RFFNCWSNHGVVDMAKAIYQSCDSYFYHFAQQVGFEKIAAFAKKMGLGQEFPLPVTSQFY
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCHHHC
GTVPSPAWKEEKYGDPWRAFDTVNASIGQGYYLASPLQLAVMSARLATGKALMPRLTLDG
CCCCCCCCCCCCCCCCHHHHHHCCCCCCCCEEECCHHHHHHHHHHHHCCCHHCCEEEECC
KTPQPASLGFSQDHIAHVRQAMSDVVNGPGTAGRARLPLEEVKMAGKTGTAQVVSLSISD
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCEEEEEEEECC
GRSGPWKYRDHGLFVFFAPLDKPKYAGAVVIEHGGGSGAAYPIARDVMTFLFDPAKGLEA
CCCCCCEECCCCEEEEEECCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCHHHHHH
LNALEEQWGGTAQQRLVRKYAAYAAERGAEVEPAPRRAEEIFDRVDAEARMAAQQAEALG
HHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHCC
QEASISRNESARGTEPAGGNR
CHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ALGSSHLPVSSNTLKDRFDRRSFTLGTLGLGIGTLLAVRMGYIAIAENERYAVESESNR
CCCCCCCCCCCCHHHHHHCCCCEEEHHHHHHHHHHHHHHHCEEEEECCCEEEEECCCCE
VNLTLIPPRRGWLLDRNGAPLASNRADFRVDIIPERMTDKMETLNELGELLDLDANRMRD
EEEEEECCCCCEEECCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCHHHHHH
LRKEIEESRGYAPIEIASGLDYEQFAAVSLRVPDMPGVVPQRGFSRFYPTGPSVGHLLGY
HHHHHHHHCCCCCEEECCCCCHHHHEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHC
VGPASAEEYEEDRNPLLITPGYKIGKDGLEKQFEQTLRGEPGARRVEVTASGRIVRDLST
CCCCCHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCEEECCCC
RPDVQGNPVKLTIDGPLQDYAARRIGLESGSVVVMDCDTGDLLCMASMPSFDPNSFSEGI
CCCCCCCCEEEEECCCHHHHHHHHCCCCCCCEEEEECCCCCEEEEECCCCCCCCCHHHHC
GSVEYAMLREDERVPLRNKVLKGLYPPGSTIKPMHCMEFLQEGISPDETIVCGGGRRIGN
CCHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCHHHHH
RFFNCWSNHGVVDMAKAIYQSCDSYFYHFAQQVGFEKIAAFAKKMGLGQEFPLPVTSQFY
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCHHHC
GTVPSPAWKEEKYGDPWRAFDTVNASIGQGYYLASPLQLAVMSARLATGKALMPRLTLDG
CCCCCCCCCCCCCCCCHHHHHHCCCCCCCCEEECCHHHHHHHHHHHHCCCHHCCEEEECC
KTPQPASLGFSQDHIAHVRQAMSDVVNGPGTAGRARLPLEEVKMAGKTGTAQVVSLSISD
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCEEEEEEEECC
GRSGPWKYRDHGLFVFFAPLDKPKYAGAVVIEHGGGSGAAYPIARDVMTFLFDPAKGLEA
CCCCCCEECCCCEEEEEECCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCHHHHHH
LNALEEQWGGTAQQRLVRKYAAYAAERGAEVEPAPRRAEEIFDRVDAEARMAAQQAEALG
HHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHCC
QEASISRNESARGTEPAGGNR
CHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677609; 8930920 [H]