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The map label for this gene is ywlF [H]

Identifier: 85373958

GI number: 85373958

Start: 1169258

End: 1169704

Strand: Reverse

Name: ywlF [H]

Synonym: ELI_05655

Alternate gene names: 85373958

Gene position: 1169704-1169258 (Counterclockwise)

Preceding gene: 85373959

Following gene: 85373957

Centisome position: 38.32

GC content: 65.1

Gene sequence:

>447_bases
ATGGCGACGCGCATGAAGATAGCGATCGCATCCGACCACGCCGCCGTCGAGCTCAAGGCCGAACTGCGCGACTGGCTCAT
CGAGCAGGGCCACGAGGTTGCCGACCTCGGACCGGACACGACCGACAGCGTCGATTATCCCGACTTCGGCTACAAGCTTG
CGGGCGTTGTTGCCGATGGCACAGCTGAGCGCGGTGTCGCGCTGTGCGGTAGCGGGATCGGCATTTCCATGTCGGTCAAT
CGCAATCCGGCGGTGCGTTGCGCACTGGTCGGCGAGCCGCTTTCCGCCGCCCTCTCGCGCGAACACAACGATGCAAATGT
CATCGCCATGGGCGCGCGCCTGACGGGCAGCGACATGGCCAAGGCCTGCCTCACCGCCTTCCTCGAAACCGAATTCGGCG
GCGGTCGTCACCAGCGCCGCGTCGACAAACTCTCCAACCCGAACTGA

Upstream 100 bases:

>100_bases
GGCGTCTGCCGTTCCGACTGTCCGAAATACGGAGCGCTCCCGGCGTCCTGATTGAAGATAAGGCCTCCGGACGGGGTCGC
ACGAATCGCCCCGCTCGCCT

Downstream 100 bases:

>100_bases
GATCCGCCATGAGCACAGCCGAACAGACCCAGTCCGACATCATGCACCGTTTCTGGCACGACGATCTTGCTGCCGCCGAT
CCGGAAATCGCCGAAGCCGT

Product: ribose 5-phosphate isomerase

Products: D-ribulose 5-phosphate

Alternate protein names: NA

Number of amino acids: Translated: 148; Mature: 147

Protein sequence:

>148_residues
MATRMKIAIASDHAAVELKAELRDWLIEQGHEVADLGPDTTDSVDYPDFGYKLAGVVADGTAERGVALCGSGIGISMSVN
RNPAVRCALVGEPLSAALSREHNDANVIAMGARLTGSDMAKACLTAFLETEFGGGRHQRRVDKLSNPN

Sequences:

>Translated_148_residues
MATRMKIAIASDHAAVELKAELRDWLIEQGHEVADLGPDTTDSVDYPDFGYKLAGVVADGTAERGVALCGSGIGISMSVN
RNPAVRCALVGEPLSAALSREHNDANVIAMGARLTGSDMAKACLTAFLETEFGGGRHQRRVDKLSNPN
>Mature_147_residues
ATRMKIAIASDHAAVELKAELRDWLIEQGHEVADLGPDTTDSVDYPDFGYKLAGVVADGTAERGVALCGSGIGISMSVNR
NPAVRCALVGEPLSAALSREHNDANVIAMGARLTGSDMAKACLTAFLETEFGGGRHQRRVDKLSNPN

Specific function: In Addition To Its Activity On D-Ribose 5-Phosphate It Probably Also Has Activity On D-Allose 6-Phosphate. [C]

COG id: COG0698

COG function: function code G; Ribose 5-phosphate isomerase RpiB

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lacAB/rpiB family [H]

Homologues:

Organism=Escherichia coli, GI1790528, Length=140, Percent_Identity=41.4285714285714, Blast_Score=125, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003500
- InterPro:   IPR004785 [H]

Pfam domain/function: PF02502 LacAB_rpiB [H]

EC number: 5.3.1.6

Molecular weight: Translated: 15624; Mature: 15493

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATRMKIAIASDHAAVELKAELRDWLIEQGHEVADLGPDTTDSVDYPDFGYKLAGVVADG
CCCEEEEEEECCCCEEEEHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCEEEEEEEECC
TAERGVALCGSGIGISMSVNRNPAVRCALVGEPLSAALSREHNDANVIAMGARLTGSDMA
CCCCCEEEECCCCEEEEEECCCCCEEEEEECCCHHHHHHCCCCCCEEEEECCCCCCHHHH
KACLTAFLETEFGGGRHQRRVDKLSNPN
HHHHHHHHHHCCCCCHHHHHHHHCCCCC
>Mature Secondary Structure 
ATRMKIAIASDHAAVELKAELRDWLIEQGHEVADLGPDTTDSVDYPDFGYKLAGVVADG
CCEEEEEEECCCCEEEEHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCEEEEEEEECC
TAERGVALCGSGIGISMSVNRNPAVRCALVGEPLSAALSREHNDANVIAMGARLTGSDMA
CCCCCEEEECCCCEEEEEECCCCCEEEEEECCCHHHHHHCCCCCCEEEEECCCCCCHHHH
KACLTAFLETEFGGGRHQRRVDKLSNPN
HHHHHHHHHHCCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 4.4 {D-ribose} 0.83 {D-ribose} [C]

Substrates: D-ribose 5-phosphate

Specific reaction: D-ribose 5-phosphate = D-ribulose 5-phosphate

General reaction: Intramolecular oxidoreduction; Isomerization [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]