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The map label for this gene is tgt

Identifier: 85373948

GI number: 85373948

Start: 1160959

End: 1162101

Strand: Direct

Name: tgt

Synonym: ELI_05605

Alternate gene names: 85373948

Gene position: 1160959-1162101 (Clockwise)

Preceding gene: 85373947

Following gene: 85373949

Centisome position: 38.03

GC content: 63.87

Gene sequence:

>1143_bases
ATGACCAAACCCCGCTTCGACTTCACGATCCACGCGACCGACGGCAAGGCGCGCACCGGCACCATCGCCATGCAGCGCGG
CGAAATTCGCACGCCAGCCTTCATGCCGGTGGGGACGGCTGGAACGGTCAAGGCGATGAAGCCCGAGAGCGTCCGCGCCG
CTGGCGCCGACATTATCCTCGGCAATACTTATCACCTGATGCTGCGGCCGGGCGCTGAGCGCGTTGCGCGGCTCGGTGGG
CTGCACACCTTCATGAACTGGCAGCGCCCGATCCTGACCGACAGCGGCGGCTACCAGGTGATGAGCCTGTCCGACCTGCG
CAAACTGACCGAACAAGGCGTCGAGTTCCGCAGCCATATCGACGGTTCCAAACACATGCTCACACCCGAGCGTTCGATGG
AGATCCAGCGCCTGCTCGGCTCCGATATCGTCATGGCGTTCGATGAATGTCCGCGCGCCGACCAGCCGCGCGATGTGATT
GCCGAAAGCATGGAAATGTCGATGCGCTGGGCTAAGCGCAGCCGCGACGGGTTCGACGGCGGGGCAGAGCACGCCGCCCG
TTCGGCACTGTTCGGTATCCAGCAAGGCGCGCTCGATGAGCAATTGCGCAAGATCAGCGCCGAAAGGCTCACCGATATCG
GCTTCGACGGCTATGCTATCGGCGGACTGGCTGTGGGCGAGGGGCAGGAAGCGATGTTTGCAACGCTCGATTTTGCACCA
GACCAGCTGCCGGAGGATCGCCCGCGTTATTTGATGGGTGTCGGCAAGCCGGACGATCTGGTCGGCGCAGTCGAGCGCGG
GGTCGATATGTTCGATTGCGTGTTGCCGAGCCGCTCGGGCCGCAACGGGCAGGCCTTCACCTGGAATGGCCCCATCAACC
TGCGCAACGCGCGTTTCGCCGAAGACCAGGAGCCGCTCGACAGCCGCTGCGCCTGCCCCACCTGCGGCACCTATTCGCGC
GCCTACATCCACCACCTTATCAAGTCGCAGGAAATCCTCGGCGCGATGCTGATGACCGAGCACAATATCGGCTTCTACCA
GCAGTTGATGCAGGCGATGCGCGATGCGATCGGCGAAGGGCGGTTTGCTGCCTTCGCTGCGGATTTCCGGCGCGACTACC
TGGGCGGCGGCGACACTGCATAA

Upstream 100 bases:

>100_bases
TTCCTTGCCGTCTGCGTCGGTATCATCGCCTTCATCTTCAAGACCGGGTGGCGGCTTCGGGACTAGTTTCCGCGCGGCAC
ACGCGCTAGGCGCGCCGCCA

Downstream 100 bases:

>100_bases
TCCCTCTTTTCAAATGCTTGGTCCCAGCTAAACTGGGCCAGCTTAACGGGGAACAGGGTCTGTTTATGAAATACTGCTCG
TCACTTGCTGCGCTTGCCGC

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MTKPRFDFTIHATDGKARTGTIAMQRGEIRTPAFMPVGTAGTVKAMKPESVRAAGADIILGNTYHLMLRPGAERVARLGG
LHTFMNWQRPILTDSGGYQVMSLSDLRKLTEQGVEFRSHIDGSKHMLTPERSMEIQRLLGSDIVMAFDECPRADQPRDVI
AESMEMSMRWAKRSRDGFDGGAEHAARSALFGIQQGALDEQLRKISAERLTDIGFDGYAIGGLAVGEGQEAMFATLDFAP
DQLPEDRPRYLMGVGKPDDLVGAVERGVDMFDCVLPSRSGRNGQAFTWNGPINLRNARFAEDQEPLDSRCACPTCGTYSR
AYIHHLIKSQEILGAMLMTEHNIGFYQQLMQAMRDAIGEGRFAAFAADFRRDYLGGGDTA

Sequences:

>Translated_380_residues
MTKPRFDFTIHATDGKARTGTIAMQRGEIRTPAFMPVGTAGTVKAMKPESVRAAGADIILGNTYHLMLRPGAERVARLGG
LHTFMNWQRPILTDSGGYQVMSLSDLRKLTEQGVEFRSHIDGSKHMLTPERSMEIQRLLGSDIVMAFDECPRADQPRDVI
AESMEMSMRWAKRSRDGFDGGAEHAARSALFGIQQGALDEQLRKISAERLTDIGFDGYAIGGLAVGEGQEAMFATLDFAP
DQLPEDRPRYLMGVGKPDDLVGAVERGVDMFDCVLPSRSGRNGQAFTWNGPINLRNARFAEDQEPLDSRCACPTCGTYSR
AYIHHLIKSQEILGAMLMTEHNIGFYQQLMQAMRDAIGEGRFAAFAADFRRDYLGGGDTA
>Mature_379_residues
TKPRFDFTIHATDGKARTGTIAMQRGEIRTPAFMPVGTAGTVKAMKPESVRAAGADIILGNTYHLMLRPGAERVARLGGL
HTFMNWQRPILTDSGGYQVMSLSDLRKLTEQGVEFRSHIDGSKHMLTPERSMEIQRLLGSDIVMAFDECPRADQPRDVIA
ESMEMSMRWAKRSRDGFDGGAEHAARSALFGIQQGALDEQLRKISAERLTDIGFDGYAIGGLAVGEGQEAMFATLDFAPD
QLPEDRPRYLMGVGKPDDLVGAVERGVDMFDCVLPSRSGRNGQAFTWNGPINLRNARFAEDQEPLDSRCACPTCGTYSRA
YIHHLIKSQEILGAMLMTEHNIGFYQQLMQAMRDAIGEGRFAAFAADFRRDYLGGGDTA

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=361, Percent_Identity=43.4903047091413, Blast_Score=309, Evalue=3e-84,
Organism=Escherichia coli, GI1786607, Length=362, Percent_Identity=57.182320441989, Blast_Score=421, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17544680, Length=369, Percent_Identity=41.7344173441734, Blast_Score=278, Evalue=4e-75,
Organism=Caenorhabditis elegans, GI25146502, Length=196, Percent_Identity=25, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI19920510, Length=367, Percent_Identity=42.7792915531335, Blast_Score=310, Evalue=1e-84,
Organism=Drosophila melanogaster, GI21355311, Length=155, Percent_Identity=27.0967741935484, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 41893; Mature: 41762

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKPRFDFTIHATDGKARTGTIAMQRGEIRTPAFMPVGTAGTVKAMKPESVRAAGADIIL
CCCCCEEEEEEECCCCCCCCEEEEECCCCCCCCEEECCCCCCEECCCCCHHHHCCCEEEE
GNTYHLMLRPGAERVARLGGLHTFMNWQRPILTDSGGYQVMSLSDLRKLTEQGVEFRSHI
CCEEEEEECCCHHHHHHHCCHHHHHCCCCCEEECCCCEEEEEHHHHHHHHHHHHHHHHHC
DGSKHMLTPERSMEIQRLLGSDIVMAFDECPRADQPRDVIAESMEMSMRWAKRSRDGFDG
CCCCCCCCCHHHHHHHHHHCCHHEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
GAEHAARSALFGIQQGALDEQLRKISAERLTDIGFDGYAIGGLAVGEGQEAMFATLDFAP
CHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCEEECCEEECCCCCEEEEEECCCC
DQLPEDRPRYLMGVGKPDDLVGAVERGVDMFDCVLPSRSGRNGQAFTWNGPINLRNARFA
CCCCCCCCCEEEECCCCHHHHHHHHHCCHHHHHHCCCCCCCCCCEEEECCCEECCCCCCC
EDQEPLDSRCACPTCGTYSRAYIHHLIKSQEILGAMLMTEHNIGFYQQLMQAMRDAIGEG
CCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
RFAAFAADFRRDYLGGGDTA
CHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TKPRFDFTIHATDGKARTGTIAMQRGEIRTPAFMPVGTAGTVKAMKPESVRAAGADIIL
CCCCEEEEEEECCCCCCCCEEEEECCCCCCCCEEECCCCCCEECCCCCHHHHCCCEEEE
GNTYHLMLRPGAERVARLGGLHTFMNWQRPILTDSGGYQVMSLSDLRKLTEQGVEFRSHI
CCEEEEEECCCHHHHHHHCCHHHHHCCCCCEEECCCCEEEEEHHHHHHHHHHHHHHHHHC
DGSKHMLTPERSMEIQRLLGSDIVMAFDECPRADQPRDVIAESMEMSMRWAKRSRDGFDG
CCCCCCCCCHHHHHHHHHHCCHHEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
GAEHAARSALFGIQQGALDEQLRKISAERLTDIGFDGYAIGGLAVGEGQEAMFATLDFAP
CHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCEEECCEEECCCCCEEEEEECCCC
DQLPEDRPRYLMGVGKPDDLVGAVERGVDMFDCVLPSRSGRNGQAFTWNGPINLRNARFA
CCCCCCCCCEEEECCCCHHHHHHHHHCCHHHHHHCCCCCCCCCCEEEECCCEECCCCCCC
EDQEPLDSRCACPTCGTYSRAYIHHLIKSQEILGAMLMTEHNIGFYQQLMQAMRDAIGEG
CCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
RFAAFAADFRRDYLGGGDTA
CHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA