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The map label for this gene is wcaJ [H]

Identifier: 85373890

GI number: 85373890

Start: 1097973

End: 1099358

Strand: Direct

Name: wcaJ [H]

Synonym: ELI_05315

Alternate gene names: 85373890

Gene position: 1097973-1099358 (Clockwise)

Preceding gene: 85373889

Following gene: 85373891

Centisome position: 35.97

GC content: 60.1

Gene sequence:

>1386_bases
ATGATCCGCCTGTTCAAACATTATATCCCGTATGCCGTAGTACTGATGGGGCTCATCGACCTCGTCCTGCTGGTAATGGC
GGCGGAAATTGCGTGGCACGTGCGGGCTGCGCAAGTCGAGATGTCGCTTGGGACCTTGCTCGGGCGCACCTCGCTGCTGA
TGTCCTTCGCCCTGGTGGTTTGGCTCGCCATGATCTCGGTCGGGATCTACGGCACTGAGGCGGTGCGCTCGATGCGCTTC
GCGGCGGCCCGGTTGCTGGTGGCCATCAGCCTCGGCGTAATCGCGCATTCAGTACTCGATTTCCTCCTGCCAGGCGTCAC
CTTATGGCGATCGACGCTGGCCTACGCGGTGGGCCTTTCCTTTGTTGCCCTGATGGGCAATCGCATCGTGATCTCGAAAA
TGCTCGGCATGGACGCTTTTCGCAGGCGCATCGTTGTCCTCGGCGCGGGGCAGCGTGCTCAGCGATTGGCGGAACTGGCG
AAGCGCCCGGAAAGCGGATTCCAGATCGTTGCCAATATCGCGATGTCGGAAGCGGGCCGCACCATGCATCATGCCATCGC
TCGCGACGGTATCGCGGACCTCGGACAATTCGTCGCCGAACAACGCGCCACCGAAGTCGTGCTCGCAATCGAGGAGCGGC
GCAACTCGCTTCCCTTGAAGGATCTGGTGCGGGTCAAGACCAAGGGCGTCCATGTCAACGAATTCAGCAGTTTCATGGAG
CGGGAAACCGGGCGCGTCGATCTCGATACGCTCAATCCTAGCTGGCTCATCTTCTCCGACGGCTTCAGTTCGGGGCGCGC
GATCTCGAGCGTCGCCAAGCGTGCGTTCGATATTGTCGCCAGCGGATTACTGCTGCTGATCACCTTCCCGATAATCGGCC
TGTTTGCAGTGCTCGTGAAGCTGGACAGCAAGGGTCCTGCCTTCTTCCGCCAGCAGCGCGTCGGGCTCTACGGCGAGACC
TTCGACGTCATCAAGCTCCGTTCCATGCGCACGGATGCCGAGAAGGACGGCGCGAAATGGGCATCGAAGGACGATCCGCG
TATTACCCGGCTTGGCAAGTTCATCCGCAAGGTACGGATCGACGAATTGCCTCAGACCTGGAGCGTTCTCAAAGGCGAGA
TGAGCTTTGTCGGACCGCGGCCAGAGGTTCCGGCGTTCGTGGCCGAATTGGACAGGCAATTGCCCTATTATGCCGAGCGC
CACATGGTAAAGCCGGGCATCACGGGCTGGGCGCAGATCAACTATCCCTATGGCGCCAGTATCGAAGATTCCCGGCACAA
GCTCGAATACGATCTCTACTACGCGAAGAATTACACGCCGTTCCTGGATCTGCTGATTCTGCTTCAGACGCTCAGGGTGA
TCCTGTGGCCGGACGGTGCGCGATGA

Upstream 100 bases:

>100_bases
GCCCGATGACAGGCGATCCGCACGCGGTGTAAAATTTACCGACATTTCATCTTATGCCCCTAGTCGGTGATCCCTCGCAG
AAGGGAAACCGACCAGGGCC

Downstream 100 bases:

>100_bases
TGACGGGCTCCGGCTTGTGGGCATTTCTCGTCATGCTGGTGCACTTGCTGAGTGCGGCGGGTTGCATCGTGGCGGCCGCC
TGGGTGATCAAGCGCCCGCT

Product: sugar transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MIRLFKHYIPYAVVLMGLIDLVLLVMAAEIAWHVRAAQVEMSLGTLLGRTSLLMSFALVVWLAMISVGIYGTEAVRSMRF
AAARLLVAISLGVIAHSVLDFLLPGVTLWRSTLAYAVGLSFVALMGNRIVISKMLGMDAFRRRIVVLGAGQRAQRLAELA
KRPESGFQIVANIAMSEAGRTMHHAIARDGIADLGQFVAEQRATEVVLAIEERRNSLPLKDLVRVKTKGVHVNEFSSFME
RETGRVDLDTLNPSWLIFSDGFSSGRAISSVAKRAFDIVASGLLLLITFPIIGLFAVLVKLDSKGPAFFRQQRVGLYGET
FDVIKLRSMRTDAEKDGAKWASKDDPRITRLGKFIRKVRIDELPQTWSVLKGEMSFVGPRPEVPAFVAELDRQLPYYAER
HMVKPGITGWAQINYPYGASIEDSRHKLEYDLYYAKNYTPFLDLLILLQTLRVILWPDGAR

Sequences:

>Translated_461_residues
MIRLFKHYIPYAVVLMGLIDLVLLVMAAEIAWHVRAAQVEMSLGTLLGRTSLLMSFALVVWLAMISVGIYGTEAVRSMRF
AAARLLVAISLGVIAHSVLDFLLPGVTLWRSTLAYAVGLSFVALMGNRIVISKMLGMDAFRRRIVVLGAGQRAQRLAELA
KRPESGFQIVANIAMSEAGRTMHHAIARDGIADLGQFVAEQRATEVVLAIEERRNSLPLKDLVRVKTKGVHVNEFSSFME
RETGRVDLDTLNPSWLIFSDGFSSGRAISSVAKRAFDIVASGLLLLITFPIIGLFAVLVKLDSKGPAFFRQQRVGLYGET
FDVIKLRSMRTDAEKDGAKWASKDDPRITRLGKFIRKVRIDELPQTWSVLKGEMSFVGPRPEVPAFVAELDRQLPYYAER
HMVKPGITGWAQINYPYGASIEDSRHKLEYDLYYAKNYTPFLDLLILLQTLRVILWPDGAR
>Mature_461_residues
MIRLFKHYIPYAVVLMGLIDLVLLVMAAEIAWHVRAAQVEMSLGTLLGRTSLLMSFALVVWLAMISVGIYGTEAVRSMRF
AAARLLVAISLGVIAHSVLDFLLPGVTLWRSTLAYAVGLSFVALMGNRIVISKMLGMDAFRRRIVVLGAGQRAQRLAELA
KRPESGFQIVANIAMSEAGRTMHHAIARDGIADLGQFVAEQRATEVVLAIEERRNSLPLKDLVRVKTKGVHVNEFSSFME
RETGRVDLDTLNPSWLIFSDGFSSGRAISSVAKRAFDIVASGLLLLITFPIIGLFAVLVKLDSKGPAFFRQQRVGLYGET
FDVIKLRSMRTDAEKDGAKWASKDDPRITRLGKFIRKVRIDELPQTWSVLKGEMSFVGPRPEVPAFVAELDRQLPYYAER
HMVKPGITGWAQINYPYGASIEDSRHKLEYDLYYAKNYTPFLDLLILLQTLRVILWPDGAR

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=330, Percent_Identity=32.4242424242424, Blast_Score=148, Evalue=7e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 51498; Mature: 51498

Theoretical pI: Translated: 10.25; Mature: 10.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRLFKHYIPYAVVLMGLIDLVLLVMAAEIAWHVRAAQVEMSLGTLLGRTSLLMSFALVV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WLAMISVGIYGTEAVRSMRFAAARLLVAISLGVIAHSVLDFLLPGVTLWRSTLAYAVGLS
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
FVALMGNRIVISKMLGMDAFRRRIVVLGAGQRAQRLAELAKRPESGFQIVANIAMSEAGR
HHHHHCCHHHHHHHHCHHHHHCEEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCH
TMHHAIARDGIADLGQFVAEQRATEVVLAIEERRNSLPLKDLVRVKTKGVHVNEFSSFME
HHHHHHHHCCHHHHHHHHHHHHHHHHHHEEHHHCCCCCHHHHHHHHHCCCCHHHHHHHHH
RETGRVDLDTLNPSWLIFSDGFSSGRAISSVAKRAFDIVASGLLLLITFPIIGLFAVLVK
HCCCCEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDSKGPAFFRQQRVGLYGETFDVIKLRSMRTDAEKDGAKWASKDDPRITRLGKFIRKVRI
HCCCCCHHHHHHHCCCCCCCHHHHHHHHHCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHH
DELPQTWSVLKGEMSFVGPRPEVPAFVAELDRQLPYYAERHMVKPGITGWAQINYPYGAS
HCCCHHHHHHHCHHHHCCCCCCCCHHHHHHHHCCCHHHHHCCCCCCCCCEEEECCCCCCC
IEDSRHKLEYDLYYAKNYTPFLDLLILLQTLRVILWPDGAR
CCCCCHHEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MIRLFKHYIPYAVVLMGLIDLVLLVMAAEIAWHVRAAQVEMSLGTLLGRTSLLMSFALVV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WLAMISVGIYGTEAVRSMRFAAARLLVAISLGVIAHSVLDFLLPGVTLWRSTLAYAVGLS
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
FVALMGNRIVISKMLGMDAFRRRIVVLGAGQRAQRLAELAKRPESGFQIVANIAMSEAGR
HHHHHCCHHHHHHHHCHHHHHCEEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCH
TMHHAIARDGIADLGQFVAEQRATEVVLAIEERRNSLPLKDLVRVKTKGVHVNEFSSFME
HHHHHHHHCCHHHHHHHHHHHHHHHHHHEEHHHCCCCCHHHHHHHHHCCCCHHHHHHHHH
RETGRVDLDTLNPSWLIFSDGFSSGRAISSVAKRAFDIVASGLLLLITFPIIGLFAVLVK
HCCCCEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDSKGPAFFRQQRVGLYGETFDVIKLRSMRTDAEKDGAKWASKDDPRITRLGKFIRKVRI
HCCCCCHHHHHHHCCCCCCCHHHHHHHHHCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHH
DELPQTWSVLKGEMSFVGPRPEVPAFVAELDRQLPYYAERHMVKPGITGWAQINYPYGAS
HCCCHHHHHHHCHHHHCCCCCCCCHHHHHHHHCCCHHHHHCCCCCCCCCEEEECCCCCCC
IEDSRHKLEYDLYYAKNYTPFLDLLILLQTLRVILWPDGAR
CCCCCHHEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]