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The map label for this gene is gap [H]

Identifier: 85373869

GI number: 85373869

Start: 1079081

End: 1080088

Strand: Direct

Name: gap [H]

Synonym: ELI_05210

Alternate gene names: 85373869

Gene position: 1079081-1080088 (Clockwise)

Preceding gene: 85373868

Following gene: 85373870

Centisome position: 35.35

GC content: 61.61

Gene sequence:

>1008_bases
ATGGCTACCAAAGTTGCCATCAACGGTTTCGGACGTATCGGCCGCCTCGTGGCGCGCGCCATTCTCGAGCGCGACGATCA
CGATCTCGACCTCGTCAGCATCAATGACCTTGCCGACACCAAATCCAACGCACTGCTGTTCCAGTACGATTCCACCCACG
GTCGCTTCCCCGGCACCGTCGAAGTCGGCGACAATGCCATCATCGTGAACGGCAAGAGCATTGCCGTGACCAGCGAGCGC
GATCCGGGCAACTTGCCGCATGGCGATCAGGGCATCGACATCGTCCTCGAATGCACCGGCTTCTTCCAGAGCCATGAGGC
TGCCGAACCGCACCTCAAGGCTGGCGCAAAGCGCGTTCTGATCTCGGCTCCGGCCAAGAATGTCTCGGCTACCATCGTCT
ACGGGGTGAATCACGATGTGCTGACCAGCGACGACGTGATCGTCTCCAACGCCAGCTGCACGACCAACTGCCTCTCACCG
ATGGCCAAGGTGCTGCACGACACGGTGGGCATCGAGCGCGGCTTCATGACGACGATCCACAGCTACACCAACGACCAGCG
CATGCTCGACCAGATGCATGGCGACATGCGCCGGGCCCGTGGCGGCGCTCAGAACATGATCCCGACCACGACGGGCGCTG
CCCGTGCAGTCGGCCTGGTCCTGCCGGAACTTGCCGGCAAGCTCGACGGCAGCTCTGTTCGCGTGCCGACGCCCAATGTG
AGCCTCGTCGACTTGGTCTTCACCCCGGGGCGGGACACCTGCGCCGAGGAACTGAACGACGCGCTTAAGGCCGCTGCAAA
AGGCAAGATGAAAGGTGTGCTCGACTACACCGATCAGCCGCTCGTCAGCTCGGACTTCAATCACTATCCGGCCAGCTCGA
CCATCGACAGCCTTGAAACCAGCGTGATGGAAGGCAAGCTCGCCCGCGTCGTAAGTTGGTATGATAACGAATGGGGCTTC
TCCAACCGCATGATCGACACCGCCGGTGTCATGGCGGGGCTGCTTTAA

Upstream 100 bases:

>100_bases
TCCGCTCCGGCAGGCGACCTGTTCGAAAAATTCGGTTTCACTGCGGACGCCATCGTCCCGCAAATACTCAACAAGATCAA
AGCATAAGCAGGAGTTCTTC

Downstream 100 bases:

>100_bases
GGAGGCTTTCGTGCCGACATTCAAGACCCTGGACGACCTTGGCGATATCACCGGCAAGGTCGCGCTGGTGCGCGTCGATC
TCAACCTGCCGATGAAGGAT

Product: glyceraldehyde 3-phosphate dehydrogenase

Products: NA

Alternate protein names: GAPDH [H]

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MATKVAINGFGRIGRLVARAILERDDHDLDLVSINDLADTKSNALLFQYDSTHGRFPGTVEVGDNAIIVNGKSIAVTSER
DPGNLPHGDQGIDIVLECTGFFQSHEAAEPHLKAGAKRVLISAPAKNVSATIVYGVNHDVLTSDDVIVSNASCTTNCLSP
MAKVLHDTVGIERGFMTTIHSYTNDQRMLDQMHGDMRRARGGAQNMIPTTTGAARAVGLVLPELAGKLDGSSVRVPTPNV
SLVDLVFTPGRDTCAEELNDALKAAAKGKMKGVLDYTDQPLVSSDFNHYPASSTIDSLETSVMEGKLARVVSWYDNEWGF
SNRMIDTAGVMAGLL

Sequences:

>Translated_335_residues
MATKVAINGFGRIGRLVARAILERDDHDLDLVSINDLADTKSNALLFQYDSTHGRFPGTVEVGDNAIIVNGKSIAVTSER
DPGNLPHGDQGIDIVLECTGFFQSHEAAEPHLKAGAKRVLISAPAKNVSATIVYGVNHDVLTSDDVIVSNASCTTNCLSP
MAKVLHDTVGIERGFMTTIHSYTNDQRMLDQMHGDMRRARGGAQNMIPTTTGAARAVGLVLPELAGKLDGSSVRVPTPNV
SLVDLVFTPGRDTCAEELNDALKAAAKGKMKGVLDYTDQPLVSSDFNHYPASSTIDSLETSVMEGKLARVVSWYDNEWGF
SNRMIDTAGVMAGLL
>Mature_334_residues
ATKVAINGFGRIGRLVARAILERDDHDLDLVSINDLADTKSNALLFQYDSTHGRFPGTVEVGDNAIIVNGKSIAVTSERD
PGNLPHGDQGIDIVLECTGFFQSHEAAEPHLKAGAKRVLISAPAKNVSATIVYGVNHDVLTSDDVIVSNASCTTNCLSPM
AKVLHDTVGIERGFMTTIHSYTNDQRMLDQMHGDMRRARGGAQNMIPTTTGAARAVGLVLPELAGKLDGSSVRVPTPNVS
LVDLVFTPGRDTCAEELNDALKAAAKGKMKGVLDYTDQPLVSSDFNHYPASSTIDSLETSVMEGKLARVVSWYDNEWGFS
NRMIDTAGVMAGLL

Specific function: Second phase of glycolysis; first step. [C]

COG id: COG0057

COG function: function code G; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI7669492, Length=331, Percent_Identity=47.1299093655589, Blast_Score=305, Evalue=4e-83,
Organism=Homo sapiens, GI7657116, Length=328, Percent_Identity=45.1219512195122, Blast_Score=275, Evalue=5e-74,
Organism=Escherichia coli, GI1788079, Length=327, Percent_Identity=51.6819571865443, Blast_Score=333, Evalue=7e-93,
Organism=Escherichia coli, GI1789295, Length=334, Percent_Identity=46.1077844311377, Blast_Score=303, Evalue=9e-84,
Organism=Caenorhabditis elegans, GI17534677, Length=336, Percent_Identity=47.0238095238095, Blast_Score=289, Evalue=1e-78,
Organism=Caenorhabditis elegans, GI17534679, Length=336, Percent_Identity=46.7261904761905, Blast_Score=289, Evalue=1e-78,
Organism=Caenorhabditis elegans, GI32566163, Length=336, Percent_Identity=47.6190476190476, Blast_Score=288, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI17568413, Length=336, Percent_Identity=47.6190476190476, Blast_Score=288, Evalue=3e-78,
Organism=Saccharomyces cerevisiae, GI6321631, Length=325, Percent_Identity=50.1538461538461, Blast_Score=314, Evalue=1e-86,
Organism=Saccharomyces cerevisiae, GI6322468, Length=325, Percent_Identity=49.8461538461538, Blast_Score=313, Evalue=3e-86,
Organism=Saccharomyces cerevisiae, GI6322409, Length=325, Percent_Identity=48.6153846153846, Blast_Score=308, Evalue=8e-85,
Organism=Drosophila melanogaster, GI85725000, Length=331, Percent_Identity=47.4320241691843, Blast_Score=301, Evalue=5e-82,
Organism=Drosophila melanogaster, GI22023983, Length=331, Percent_Identity=47.4320241691843, Blast_Score=301, Evalue=5e-82,
Organism=Drosophila melanogaster, GI17933600, Length=331, Percent_Identity=47.1299093655589, Blast_Score=300, Evalue=1e-81,
Organism=Drosophila melanogaster, GI18110149, Length=331, Percent_Identity=47.1299093655589, Blast_Score=300, Evalue=1e-81,
Organism=Drosophila melanogaster, GI19922412, Length=324, Percent_Identity=43.5185185185185, Blast_Score=275, Evalue=4e-74,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1840 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 740 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Min

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020831
- InterPro:   IPR020830
- InterPro:   IPR020829
- InterPro:   IPR020828
- InterPro:   IPR006424
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02800 Gp_dh_C; PF00044 Gp_dh_N [H]

EC number: =1.2.1.12 [H]

Molecular weight: Translated: 35868; Mature: 35737

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00071 GAPDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATKVAINGFGRIGRLVARAILERDDHDLDLVSINDLADTKSNALLFQYDSTHGRFPGTV
CCCEEEECCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEEEECCCCCCCCCEE
EVGDNAIIVNGKSIAVTSERDPGNLPHGDQGIDIVLECTGFFQSHEAAEPHLKAGAKRVL
EECCCEEEEECCEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHCCCCEEE
ISAPAKNVSATIVYGVNHDVLTSDDVIVSNASCTTNCLSPMAKVLHDTVGIERGFMTTIH
EECCCCCCEEEEEEECCCCEECCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHH
SYTNDQRMLDQMHGDMRRARGGAQNMIPTTTGAARAVGLVLPELAGKLDGSSVRVPTPNV
HHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCC
SLVDLVFTPGRDTCAEELNDALKAAAKGKMKGVLDYTDQPLVSSDFNHYPASSTIDSLET
EEEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHH
SVMEGKLARVVSWYDNEWGFSNRMIDTAGVMAGLL
HHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
ATKVAINGFGRIGRLVARAILERDDHDLDLVSINDLADTKSNALLFQYDSTHGRFPGTV
CCEEEECCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEEEECCCCCCCCCEE
EVGDNAIIVNGKSIAVTSERDPGNLPHGDQGIDIVLECTGFFQSHEAAEPHLKAGAKRVL
EECCCEEEEECCEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHCCCCEEE
ISAPAKNVSATIVYGVNHDVLTSDDVIVSNASCTTNCLSPMAKVLHDTVGIERGFMTTIH
EECCCCCCEEEEEEECCCCEECCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHH
SYTNDQRMLDQMHGDMRRARGGAQNMIPTTTGAARAVGLVLPELAGKLDGSSVRVPTPNV
HHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCC
SLVDLVFTPGRDTCAEELNDALKAAAKGKMKGVLDYTDQPLVSSDFNHYPASSTIDSLET
EEEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHH
SVMEGKLARVVSWYDNEWGFSNRMIDTAGVMAGLL
HHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3680173 [H]