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The map label for this gene is pgk

Identifier: 85373870

GI number: 85373870

Start: 1080099

End: 1081298

Strand: Direct

Name: pgk

Synonym: ELI_05215

Alternate gene names: 85373870

Gene position: 1080099-1081298 (Clockwise)

Preceding gene: 85373869

Following gene: 85373871

Centisome position: 35.39

GC content: 63.92

Gene sequence:

>1200_bases
GTGCCGACATTCAAGACCCTGGACGACCTTGGCGATATCACCGGCAAGGTCGCGCTGGTGCGCGTCGATCTCAACCTGCC
GATGAAGGATGGTTCGGCGAGCGACGTCACCCGTGTCGAAGCCGTCAAGCCGACCATTCTCGAACTGAGCGAGAAAGGCG
CGAAAGTCCTGCTCCTCGCCCATTTCGGGCGTCCGGGCGGGAAGCGCTCGTCGGTCCTTTCGACCAGCATGGTGGTGGGC
GATGTCGAAGGCGTGCTCGGCAAGGAACTGATGTTCATTCCCGAGGTCTCGGGACCGGTTGTCGAGCAATCGATCGGCAT
CCTGCGCAATGGCGACATCGGCGTGCTCGACAATACGCGTTTCTGGCCCGGCGAGACGGACAATGATCCTGATTTTGCCA
AGGCCATCGCCGCGCACGGCGATTTCTATGTCAACGACGCGTTTTCCGCCGCACATCGCGCCCATGCGACGACAGAAGGC
ATCACGCATTTCCTGCCATCCTATGCAGGCCGCGCGATGGAGAGGGAATTGAAAGCGCTCGATTCCGCACTCGGTAATCC
CGAAGCGCCGGTCGCGGCCGTCGTCGGCGGGGCCAAGGTTTCGACCAAGCTCGACGTGCTCAAAAACCTGGTCGGGCGCG
TCCAGCACCTGATCATCGGCGGCGGCATGGCCAACACCTTTCTTGCCGCGCGCGGGGTCGATGTCGGCAAGAGCTTGTGC
GAGCACGACCTCACCGACACCGCCAGTGCCATCATGGACGAGGCCGATCACGCCGGCTGCACGGTGCACCTGCCCTATGA
CGTCGTGGTGGCGAAGGAGTTCGCGGCCAATCCGCCGAGCGTGCGGACCTGCAATGTCCACGAGGTCGCCGCGGATGAAA
TGATCCTCGACGTCGGTCCGCAGGCGACCGAGGCGCTGGGCGATGTGCTCAAGACCTGCCGCACGCTGGTATGGAACGGC
CCCCTCGGTGCCTTTGAAATCGAGCCCTTCGATGCGGCGACAGTTGCATTGGCAAAGACCGCGGCCGCGCTGACGCAGGA
TGGCTCGCTCGTCTCCGTCGCAGGCGGAGGCGACACCGTGGCGGCGCTGGCGCATGCGGGCGTCACAGAAGATGTGACTT
ACGTTTCTACCGCAGGCGGGGCCTTCCTCGAGTGGATGGAGGGCAAGGAACTGCCGGGGGTCAAGGCACTGAGCGCGTGA

Upstream 100 bases:

>100_bases
CTCGCCCGCGTCGTAAGTTGGTATGATAACGAATGGGGCTTCTCCAACCGCATGATCGACACCGCCGGTGTCATGGCGGG
GCTGCTTTAAGGAGGCTTTC

Downstream 100 bases:

>100_bases
GCGAGAACGAAGCTGTAGGTTCCAGTGGCGGGCTGGTCCCGATCACCGAGGGCGAATGGGCCGGGTGGTCGACCTGGCAG
GGCGATGCCTTCGAACAGCG

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MPTFKTLDDLGDITGKVALVRVDLNLPMKDGSASDVTRVEAVKPTILELSEKGAKVLLLAHFGRPGGKRSSVLSTSMVVG
DVEGVLGKELMFIPEVSGPVVEQSIGILRNGDIGVLDNTRFWPGETDNDPDFAKAIAAHGDFYVNDAFSAAHRAHATTEG
ITHFLPSYAGRAMERELKALDSALGNPEAPVAAVVGGAKVSTKLDVLKNLVGRVQHLIIGGGMANTFLAARGVDVGKSLC
EHDLTDTASAIMDEADHAGCTVHLPYDVVVAKEFAANPPSVRTCNVHEVAADEMILDVGPQATEALGDVLKTCRTLVWNG
PLGAFEIEPFDAATVALAKTAAALTQDGSLVSVAGGGDTVAALAHAGVTEDVTYVSTAGGAFLEWMEGKELPGVKALSA

Sequences:

>Translated_399_residues
MPTFKTLDDLGDITGKVALVRVDLNLPMKDGSASDVTRVEAVKPTILELSEKGAKVLLLAHFGRPGGKRSSVLSTSMVVG
DVEGVLGKELMFIPEVSGPVVEQSIGILRNGDIGVLDNTRFWPGETDNDPDFAKAIAAHGDFYVNDAFSAAHRAHATTEG
ITHFLPSYAGRAMERELKALDSALGNPEAPVAAVVGGAKVSTKLDVLKNLVGRVQHLIIGGGMANTFLAARGVDVGKSLC
EHDLTDTASAIMDEADHAGCTVHLPYDVVVAKEFAANPPSVRTCNVHEVAADEMILDVGPQATEALGDVLKTCRTLVWNG
PLGAFEIEPFDAATVALAKTAAALTQDGSLVSVAGGGDTVAALAHAGVTEDVTYVSTAGGAFLEWMEGKELPGVKALSA
>Mature_398_residues
PTFKTLDDLGDITGKVALVRVDLNLPMKDGSASDVTRVEAVKPTILELSEKGAKVLLLAHFGRPGGKRSSVLSTSMVVGD
VEGVLGKELMFIPEVSGPVVEQSIGILRNGDIGVLDNTRFWPGETDNDPDFAKAIAAHGDFYVNDAFSAAHRAHATTEGI
THFLPSYAGRAMERELKALDSALGNPEAPVAAVVGGAKVSTKLDVLKNLVGRVQHLIIGGGMANTFLAARGVDVGKSLCE
HDLTDTASAIMDEADHAGCTVHLPYDVVVAKEFAANPPSVRTCNVHEVAADEMILDVGPQATEALGDVLKTCRTLVWNGP
LGAFEIEPFDAATVALAKTAAALTQDGSLVSVAGGGDTVAALAHAGVTEDVTYVSTAGGAFLEWMEGKELPGVKALSA

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI4505763, Length=412, Percent_Identity=36.4077669902913, Blast_Score=244, Evalue=9e-65,
Organism=Homo sapiens, GI31543397, Length=412, Percent_Identity=36.6504854368932, Blast_Score=238, Evalue=6e-63,
Organism=Escherichia coli, GI1789294, Length=388, Percent_Identity=41.7525773195876, Blast_Score=271, Evalue=4e-74,
Organism=Caenorhabditis elegans, GI17508823, Length=410, Percent_Identity=37.0731707317073, Blast_Score=257, Evalue=9e-69,
Organism=Saccharomyces cerevisiae, GI6319857, Length=410, Percent_Identity=34.6341463414634, Blast_Score=228, Evalue=2e-60,
Organism=Drosophila melanogaster, GI17136394, Length=405, Percent_Identity=35.5555555555556, Blast_Score=237, Evalue=1e-62,
Organism=Drosophila melanogaster, GI24581203, Length=405, Percent_Identity=35.0617283950617, Blast_Score=229, Evalue=3e-60,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 41487; Mature: 41355

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTFKTLDDLGDITGKVALVRVDLNLPMKDGSASDVTRVEAVKPTILELSEKGAKVLLLA
CCCCCCHHHHHHCCCEEEEEEEEECCCCCCCCCCCCHHHHHCCCHHHHHHCCCCEEEEEE
HFGRPGGKRSSVLSTSMVVGDVEGVLGKELMFIPEVSGPVVEQSIGILRNGDIGVLDNTR
ECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHCCEEECCCEEEECCCC
FWPGETDNDPDFAKAIAAHGDFYVNDAFSAAHRAHATTEGITHFLPSYAGRAMERELKAL
CCCCCCCCCHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DSALGNPEAPVAAVVGGAKVSTKLDVLKNLVGRVQHLIIGGGMANTFLAARGVDVGKSLC
HHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHH
EHDLTDTASAIMDEADHAGCTVHLPYDVVVAKEFAANPPSVRTCNVHEVAADEMILDVGP
HHCHHHHHHHHHHCCCCCCEEEECCCCEEEEHHHCCCCCCCEECCHHHHHHHHEEEECCC
QATEALGDVLKTCRTLVWNGPLGAFEIEPFDAATVALAKTAAALTQDGSLVSVAGGGDTV
HHHHHHHHHHHHHHHHEECCCCCCEEECCCCHHHHHHHHHHHHHCCCCCEEEEECCCCHH
AALAHAGVTEDVTYVSTAGGAFLEWMEGKELPGVKALSA
HHHHHCCCCCCCEEEECCCCHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
PTFKTLDDLGDITGKVALVRVDLNLPMKDGSASDVTRVEAVKPTILELSEKGAKVLLLA
CCCCCHHHHHHCCCEEEEEEEEECCCCCCCCCCCCHHHHHCCCHHHHHHCCCCEEEEEE
HFGRPGGKRSSVLSTSMVVGDVEGVLGKELMFIPEVSGPVVEQSIGILRNGDIGVLDNTR
ECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHCCEEECCCEEEECCCC
FWPGETDNDPDFAKAIAAHGDFYVNDAFSAAHRAHATTEGITHFLPSYAGRAMERELKAL
CCCCCCCCCHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DSALGNPEAPVAAVVGGAKVSTKLDVLKNLVGRVQHLIIGGGMANTFLAARGVDVGKSLC
HHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHH
EHDLTDTASAIMDEADHAGCTVHLPYDVVVAKEFAANPPSVRTCNVHEVAADEMILDVGP
HHCHHHHHHHHHHCCCCCCEEEECCCCEEEEHHHCCCCCCCEECCHHHHHHHHEEEECCC
QATEALGDVLKTCRTLVWNGPLGAFEIEPFDAATVALAKTAAALTQDGSLVSVAGGGDTV
HHHHHHHHHHHHHHHHEECCCCCCEEECCCCHHHHHHHHHHHHHCCCCCEEEEECCCCHH
AALAHAGVTEDVTYVSTAGGAFLEWMEGKELPGVKALSA
HHHHHCCCCCCCEEEECCCCHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA