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The map label for this gene is thyA [H]

Identifier: 85373857

GI number: 85373857

Start: 1066805

End: 1067788

Strand: Direct

Name: thyA [H]

Synonym: ELI_05150

Alternate gene names: 85373857

Gene position: 1066805-1067788 (Clockwise)

Preceding gene: 85373856

Following gene: 85373858

Centisome position: 34.95

GC content: 59.76

Gene sequence:

>984_bases
ATGGCAAGCGTCTCGATTCCGTCCGAGCTCATGGGGCCCAGCAACTCGGAACGCCATTCCGAATGGCAATATCTCGACCT
GATGCGGCGCATCTGGGCGGAGGGCAGCGAGCGGGTCGATCGCACGGGTGTCGGCACGCGTTCGGTGTTCGGGGCACAAT
TGCGGTTCGACCTGGCCGATGGGGCTATGCCGCTGCTCACAACGAAGCGGGTCTACTGGAAAACCGCGACGCGCGAGATG
CTGTGGTTCCTGACCGGTGACACGAATATCCGCCCGCTGGTGCTGCAGGGAGTCAAGATCTGGAATGAGTGGCCGCATGC
CCGCTATGTTAACGAAACGGGCGAGGACATTGCGCTCGACGATTTCATCCAGCGCATCGCCGATGACGAGGCCTTTGCTG
AACGCTGGGGGGATCTCGGCCCGGTCTATGGCAAGCAATGGGTCGATTGGCCGACCTATCGTTATCGCAAGGACGGGCTG
TACGAGAAAAGCGAAGGCATCAACCAGATCGCCGAGGTTGTCGAAAGCCTCAAGACCAATCCTGGCAGCCGCCGCCACAT
CATCGAAGGCTGGAATGTCGCCGAGCTTGACCGCATGGCGCTGCCGCCCTGCCACAAGACCTATCAATTTCACGTCGCTA
AGGACGATCAAGGGCGCGATCGCCTGAATTGTCTGCTTTACCAGCGCAGTTGCGATGTCGCGCTCGGTCTGCCTTTCAAC
CTGTGGTCGGCAGCGCTGTTGCAGCGCATGATGGCGCAGCAGACCGACCTGGAACCGGGCGAGCTGGTCTGGATGGGCGG
AGACACGCATCTCTACCTCAATCACGAGCACTTGATCACCGAGCAGCTGAATCGGGAACCGCAAGGCCATCCCACGCTTG
AAATAGTAAGGCGGCCCGACAGCATATTCGACTACCGGATCGAGGATTTCGTGGTGCATAATTACGAACCGCACCCTCCG
ATCAAAGCACCTGTTGCGGTCTGA

Upstream 100 bases:

>100_bases
CCCGCATGGCCAATCGCCTGTGCGGGTCGAGGCAAGGCGCGCCCTATAGGTGTGGATGGGCGGGGTGGCAAGCGGGCAAT
TGCAAGGTAAGAGACTTTGC

Downstream 100 bases:

>100_bases
CCCGATCCGGGTCCATAGCCTTGACCATAAGCTCTGCGCGCAATAAATGTTCGCGCAATTGTAAGAATGCATCTGCCTGA
TTTTTCGGCAGAGGGGAGAG

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MASVSIPSELMGPSNSERHSEWQYLDLMRRIWAEGSERVDRTGVGTRSVFGAQLRFDLADGAMPLLTTKRVYWKTATREM
LWFLTGDTNIRPLVLQGVKIWNEWPHARYVNETGEDIALDDFIQRIADDEAFAERWGDLGPVYGKQWVDWPTYRYRKDGL
YEKSEGINQIAEVVESLKTNPGSRRHIIEGWNVAELDRMALPPCHKTYQFHVAKDDQGRDRLNCLLYQRSCDVALGLPFN
LWSAALLQRMMAQQTDLEPGELVWMGGDTHLYLNHEHLITEQLNREPQGHPTLEIVRRPDSIFDYRIEDFVVHNYEPHPP
IKAPVAV

Sequences:

>Translated_327_residues
MASVSIPSELMGPSNSERHSEWQYLDLMRRIWAEGSERVDRTGVGTRSVFGAQLRFDLADGAMPLLTTKRVYWKTATREM
LWFLTGDTNIRPLVLQGVKIWNEWPHARYVNETGEDIALDDFIQRIADDEAFAERWGDLGPVYGKQWVDWPTYRYRKDGL
YEKSEGINQIAEVVESLKTNPGSRRHIIEGWNVAELDRMALPPCHKTYQFHVAKDDQGRDRLNCLLYQRSCDVALGLPFN
LWSAALLQRMMAQQTDLEPGELVWMGGDTHLYLNHEHLITEQLNREPQGHPTLEIVRRPDSIFDYRIEDFVVHNYEPHPP
IKAPVAV
>Mature_326_residues
ASVSIPSELMGPSNSERHSEWQYLDLMRRIWAEGSERVDRTGVGTRSVFGAQLRFDLADGAMPLLTTKRVYWKTATREML
WFLTGDTNIRPLVLQGVKIWNEWPHARYVNETGEDIALDDFIQRIADDEAFAERWGDLGPVYGKQWVDWPTYRYRKDGLY
EKSEGINQIAEVVESLKTNPGSRRHIIEGWNVAELDRMALPPCHKTYQFHVAKDDQGRDRLNCLLYQRSCDVALGLPFNL
WSAALLQRMMAQQTDLEPGELVWMGGDTHLYLNHEHLITEQLNREPQGHPTLEIVRRPDSIFDYRIEDFVVHNYEPHPPI
KAPVAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=335, Percent_Identity=41.4925373134328, Blast_Score=244, Evalue=7e-65,
Organism=Escherichia coli, GI1789191, Length=305, Percent_Identity=51.4754098360656, Blast_Score=294, Evalue=6e-81,
Organism=Caenorhabditis elegans, GI71993377, Length=325, Percent_Identity=40.3076923076923, Blast_Score=229, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6324648, Length=317, Percent_Identity=37.2239747634069, Blast_Score=196, Evalue=3e-51,
Organism=Drosophila melanogaster, GI17137556, Length=321, Percent_Identity=42.6791277258567, Blast_Score=253, Evalue=1e-67,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 37858; Mature: 37727

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASVSIPSELMGPSNSERHSEWQYLDLMRRIWAEGSERVDRTGVGTRSVFGAQLRFDLAD
CCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHCCEEEEEECC
GAMPLLTTKRVYWKTATREMLWFLTGDTNIRPLVLQGVKIWNEWPHARYVNETGEDIALD
CCCCHHCCCHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCEECCCCCCCCCHH
DFIQRIADDEAFAERWGDLGPVYGKQWVDWPTYRYRKDGLYEKSEGINQIAEVVESLKTN
HHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCHHCCHHHHHHHHHHHHCC
PGSRRHIIEGWNVAELDRMALPPCHKTYQFHVAKDDQGRDRLNCLLYQRSCDVALGLPFN
CCCCCEEECCCCHHHHHHHCCCCCCCCEEEEEECCCCCCCHHEEEEEECCCCEEECCCHH
LWSAALLQRMMAQQTDLEPGELVWMGGDTHLYLNHEHLITEQLNREPQGHPTLEIVRRPD
HHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEECCHHHHHHHHCCCCCCCCCEEEEECCC
SIFDYRIEDFVVHNYEPHPPIKAPVAV
HHHHHHHHHEEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
ASVSIPSELMGPSNSERHSEWQYLDLMRRIWAEGSERVDRTGVGTRSVFGAQLRFDLAD
CCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHCCEEEEEECC
GAMPLLTTKRVYWKTATREMLWFLTGDTNIRPLVLQGVKIWNEWPHARYVNETGEDIALD
CCCCHHCCCHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCEECCCCCCCCCHH
DFIQRIADDEAFAERWGDLGPVYGKQWVDWPTYRYRKDGLYEKSEGINQIAEVVESLKTN
HHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCHHCCHHHHHHHHHHHHCC
PGSRRHIIEGWNVAELDRMALPPCHKTYQFHVAKDDQGRDRLNCLLYQRSCDVALGLPFN
CCCCCEEECCCCHHHHHHHCCCCCCCCEEEEEECCCCCCCHHEEEEEECCCCEEECCCHH
LWSAALLQRMMAQQTDLEPGELVWMGGDTHLYLNHEHLITEQLNREPQGHPTLEIVRRPD
HHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEECCHHHHHHHHCCCCCCCCCEEEEECCC
SIFDYRIEDFVVHNYEPHPPIKAPVAV
HHHHHHHHHEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA