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The map label for this gene is bkdA1 [H]

Identifier: 85373858

GI number: 85373858

Start: 1067895

End: 1069184

Strand: Direct

Name: bkdA1 [H]

Synonym: ELI_05155

Alternate gene names: 85373858

Gene position: 1067895-1069184 (Clockwise)

Preceding gene: 85373857

Following gene: 85373859

Centisome position: 34.99

GC content: 63.26

Gene sequence:

>1290_bases
ATGGTCGACCGGCCTGCGGGGTCCAATAAACCCCATCTCGAACTGCATGTGCCCGAGCCGAAGTTCCGGCCCGGCGACCA
GGCGGATTTCTCGCACATCGACATCGGCGAAGCCGGTGCGCAGCCGCGCCCCGATGAAGCCTGCGACGCGAAGGAAACCC
TGCCGCTCTGCTACGACCTGATCCGGGTGCTGGGCGAGGATAACAAGGCGCATGGCGCTTGGGATCCCAAGCTCGACCCT
GAGACTTTGCGCCAGATGCTGCAACATTTTGCCACCGTGCGCGCGTTTGACGAGCGCATGTTCCGCGGGCAGCGGCAGGG
CAAGACCAGCTTCTACATGAAGTGCACCGGCGAAGAAGCGACAAGCGTGTCCGCCGCCATGGCGCTCGCCAGCGACGACA
TGGTCTTCCCGAGCTATCGCCAGCAGGGTGTGCTGATCGCGCGCGGCTACCCGATGATCGAGATGATCAATCAGATCTAT
TCGAACAAGGCCGACAAGCTGAAGGGTCGCCAGCTGCCGATCATGTATTCGAGCCGGGAGCACAGCTTCTTCTCGATTTC
CGGCAACCTCGCCACGCAAACGCCGCAAGCGGTCGGTTGGGCGATGGCGAGCGCGATCAAGAACGACAGCCGGATTGCTG
CGACGTGGGTGGGTGAAGGCTCGACGGCGGAAGGTGACTTTCACTCCGCCTGCACCTTCGCGACAGTCTACAATGCGCCG
GTCATCATCAACGTAATCAACAACCAGTGGGCGATCTCGAGCTTTTCGGGCTTTGCCGGGGCTGAGCGCACGACATTTGC
AGCCCGTGCGCTGGGATATGGTCTGGCCGGCCTCCGCGTCGATGGCAACGACCCGCTCGCGGTCTATGCCGCCGAGCGCT
GGGCCGCCAACCGCGCGCGCGCCAACCAGGGACCGACGCTGATCGAGTTCTTCACCTATCGCGCCGAGGGGCACTCCACT
TCGGATGATCCCAGCGGATATCGCAGCGCGCAGGAACGCGAGGAATGGCCGCTGGGCGATCCGATCAACCGTTTGAAAAA
CCACCTGATCGAGCTCGGTGAGTGGGACGAGGATCGGCAGGCGGCGATGGACAAGGAAGCGGCGGAGAAGGTCAAGGCAA
CCACCAAGGAGGCCGAGAAGAACGGCATCCTCGGCCACGGCCTGCACCATCCGTTCCGCACCATGTTCCAGGACGTTTAC
GAAGAGCTGCCCTGGCACCTCGAAGAGCAGGCCGCGCAGGCCAAGCGCGAACGCGAGATCAAATGGCCCGGTGAGGAGCC
CTGGTCATGA

Upstream 100 bases:

>100_bases
CCGGGTCCATAGCCTTGACCATAAGCTCTGCGCGCAATAAATGTTCGCGCAATTGTAAGAATGCATCTGCCTGATTTTTC
GGCAGAGGGGAGAGTGTCGA

Downstream 100 bases:

>100_bases
GCGACGCCTTAAAAACACACGCGAGGGAAAAGCCTGTGGCCGAGCAGGGCACCGAACGCCGCCTCAACATGATCGAGGCG
ATCAACGATGCGCTCGACAT

Product: 2-oxoisovalerate dehydrogenase (alpha subunit)

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain; BCKDH E1-alpha [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MVDRPAGSNKPHLELHVPEPKFRPGDQADFSHIDIGEAGAQPRPDEACDAKETLPLCYDLIRVLGEDNKAHGAWDPKLDP
ETLRQMLQHFATVRAFDERMFRGQRQGKTSFYMKCTGEEATSVSAAMALASDDMVFPSYRQQGVLIARGYPMIEMINQIY
SNKADKLKGRQLPIMYSSREHSFFSISGNLATQTPQAVGWAMASAIKNDSRIAATWVGEGSTAEGDFHSACTFATVYNAP
VIINVINNQWAISSFSGFAGAERTTFAARALGYGLAGLRVDGNDPLAVYAAERWAANRARANQGPTLIEFFTYRAEGHST
SDDPSGYRSAQEREEWPLGDPINRLKNHLIELGEWDEDRQAAMDKEAAEKVKATTKEAEKNGILGHGLHHPFRTMFQDVY
EELPWHLEEQAAQAKREREIKWPGEEPWS

Sequences:

>Translated_429_residues
MVDRPAGSNKPHLELHVPEPKFRPGDQADFSHIDIGEAGAQPRPDEACDAKETLPLCYDLIRVLGEDNKAHGAWDPKLDP
ETLRQMLQHFATVRAFDERMFRGQRQGKTSFYMKCTGEEATSVSAAMALASDDMVFPSYRQQGVLIARGYPMIEMINQIY
SNKADKLKGRQLPIMYSSREHSFFSISGNLATQTPQAVGWAMASAIKNDSRIAATWVGEGSTAEGDFHSACTFATVYNAP
VIINVINNQWAISSFSGFAGAERTTFAARALGYGLAGLRVDGNDPLAVYAAERWAANRARANQGPTLIEFFTYRAEGHST
SDDPSGYRSAQEREEWPLGDPINRLKNHLIELGEWDEDRQAAMDKEAAEKVKATTKEAEKNGILGHGLHHPFRTMFQDVY
EELPWHLEEQAAQAKREREIKWPGEEPWS
>Mature_429_residues
MVDRPAGSNKPHLELHVPEPKFRPGDQADFSHIDIGEAGAQPRPDEACDAKETLPLCYDLIRVLGEDNKAHGAWDPKLDP
ETLRQMLQHFATVRAFDERMFRGQRQGKTSFYMKCTGEEATSVSAAMALASDDMVFPSYRQQGVLIARGYPMIEMINQIY
SNKADKLKGRQLPIMYSSREHSFFSISGNLATQTPQAVGWAMASAIKNDSRIAATWVGEGSTAEGDFHSACTFATVYNAP
VIINVINNQWAISSFSGFAGAERTTFAARALGYGLAGLRVDGNDPLAVYAAERWAANRARANQGPTLIEFFTYRAEGHST
SDDPSGYRSAQEREEWPLGDPINRLKNHLIELGEWDEDRQAAMDKEAAEKVKATTKEAEKNGILGHGLHHPFRTMFQDVY
EELPWHLEEQAAQAKREREIKWPGEEPWS

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCKDHA family [H]

Homologues:

Organism=Homo sapiens, GI11386135, Length=354, Percent_Identity=40.6779661016949, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI258645172, Length=352, Percent_Identity=40.625, Blast_Score=243, Evalue=3e-64,
Organism=Homo sapiens, GI4885543, Length=271, Percent_Identity=28.7822878228782, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI291084742, Length=294, Percent_Identity=27.891156462585, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI4505685, Length=272, Percent_Identity=29.4117647058824, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI291084744, Length=272, Percent_Identity=28.3088235294118, Blast_Score=102, Evalue=9e-22,
Organism=Homo sapiens, GI291084757, Length=272, Percent_Identity=26.8382352941176, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI86563357, Length=340, Percent_Identity=40, Blast_Score=236, Evalue=1e-62,
Organism=Caenorhabditis elegans, GI86563355, Length=340, Percent_Identity=40, Blast_Score=236, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI17536047, Length=330, Percent_Identity=27.2727272727273, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI32564172, Length=330, Percent_Identity=27.2727272727273, Blast_Score=119, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6321026, Length=295, Percent_Identity=28.4745762711864, Blast_Score=118, Evalue=1e-27,
Organism=Drosophila melanogaster, GI21355903, Length=366, Percent_Identity=36.6120218579235, Blast_Score=226, Evalue=3e-59,
Organism=Drosophila melanogaster, GI24639748, Length=353, Percent_Identity=28.6118980169972, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24639740, Length=315, Percent_Identity=27.6190476190476, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24639744, Length=330, Percent_Identity=27.5757575757576, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI28571106, Length=330, Percent_Identity=27.5757575757576, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24639746, Length=307, Percent_Identity=27.6872964169381, Blast_Score=98, Evalue=9e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017
- InterPro:   IPR022593 [H]

Pfam domain/function: PF00676 E1_dh; PF12573 OxoDH_E1alpha_N [H]

EC number: =1.2.4.4 [H]

Molecular weight: Translated: 47879; Mature: 47879

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDRPAGSNKPHLELHVPEPKFRPGDQADFSHIDIGEAGAQPRPDEACDAKETLPLCYDL
CCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHH
IRVLGEDNKAHGAWDPKLDPETLRQMLQHFATVRAFDERMFRGQRQGKTSFYMKCTGEEA
HHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCH
TSVSAAMALASDDMVFPSYRQQGVLIARGYPMIEMINQIYSNKADKLKGRQLPIMYSSRE
HHHHHHHHHCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHCCCHHHCCCCCCCEEECCCC
HSFFSISGNLATQTPQAVGWAMASAIKNDSRIAATWVGEGSTAEGDFHSACTFATVYNAP
CCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHEEECCC
VIINVINNQWAISSFSGFAGAERTTFAARALGYGLAGLRVDGNDPLAVYAAERWAANRAR
EEEEEECCCEEECCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCEEEEEHHHHHHHHHC
ANQGPTLIEFFTYRAEGHSTSDDPSGYRSAQEREEWPLGDPINRLKNHLIELGEWDEDRQ
CCCCCEEEEEEEEECCCCCCCCCCCHHHCHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHH
AAMDKEAAEKVKATTKEAEKNGILGHGLHHPFRTMFQDVYEELPWHLEEQAAQAKREREI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
KWPGEEPWS
CCCCCCCCC
>Mature Secondary Structure
MVDRPAGSNKPHLELHVPEPKFRPGDQADFSHIDIGEAGAQPRPDEACDAKETLPLCYDL
CCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHH
IRVLGEDNKAHGAWDPKLDPETLRQMLQHFATVRAFDERMFRGQRQGKTSFYMKCTGEEA
HHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCH
TSVSAAMALASDDMVFPSYRQQGVLIARGYPMIEMINQIYSNKADKLKGRQLPIMYSSRE
HHHHHHHHHCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHCCCHHHCCCCCCCEEECCCC
HSFFSISGNLATQTPQAVGWAMASAIKNDSRIAATWVGEGSTAEGDFHSACTFATVYNAP
CCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHEEECCC
VIINVINNQWAISSFSGFAGAERTTFAARALGYGLAGLRVDGNDPLAVYAAERWAANRAR
EEEEEECCCEEECCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCEEEEEHHHHHHHHHC
ANQGPTLIEFFTYRAEGHSTSDDPSGYRSAQEREEWPLGDPINRLKNHLIELGEWDEDRQ
CCCCCEEEEEEEEECCCCCCCCCCCHHHCHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHH
AAMDKEAAEKVKATTKEAEKNGILGHGLHHPFRTMFQDVYEELPWHLEEQAAQAKREREI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
KWPGEEPWS
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]