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The map label for this gene is ispB [H]

Identifier: 85373560

GI number: 85373560

Start: 755772

End: 756794

Strand: Direct

Name: ispB [H]

Synonym: ELI_03665

Alternate gene names: 85373560

Gene position: 755772-756794 (Clockwise)

Preceding gene: 85373557

Following gene: 85373561

Centisome position: 24.76

GC content: 63.54

Gene sequence:

>1023_bases
ATGACTGCCGACATCATTCCTATCAGGTCTAACGGGGACGATGCTCCGCCCACCATCGAGCCGATGCTGCAACTGACGGC
GTCGGGAATGAATGCGGTCAACACCGTGATACTCGACCGCATGCAAAGCGAGATCCCGCTGATTCCGCGCCTTGCCGGGC
ACCTGATCTCCGGGGGCGGCAAACGCTTGCGGCCGATGTTGACGCTCGCGGGGGCCGAACTGGTCGGATACCAGGGCTCG
CGGCATCACAAACTCGCCGCTGCGGTCGAATTCATTCACACGGCCACGCTGCTGCATGACGACGTCGTCGACGGGTCGGA
GATGCGGCGCGGCAAGGCGGCGGCGAATATCATATTCGGCAATCCGGCGACGGTACTCGTCGGAGATTTCCTGTTCAGTC
GCGCCTTCGAACTCATGACCGAAGACGGCAGCCTGCGGGTTCTCAAAATCCTTTCCAATGCCAGCGCGGTCATTGCCGAA
GGCGAGGTTGCGCAGCTCAGCGCCCAGCGCAAGCTGGAGACGAGCGAGGAGCGCTATCTCGACATCATCGGCGCCAAAAC
CGCCGCCCTGTTCGCAGCAGCGAGCCGTATCTCCGCCGTGGTGGCCGAATGCGACGATTCGCACGAGCGCGCGCTCGACG
ATTACGGGCGCAATCTGGGTGTCGCGTTCCAGCTTGTCGATGACGCGATCGATTACGATTCCGATGCCGCCGAAATGGGC
AAGGACCAGGGCGACGATTTCCGCGAAGGCAAGATGACCCTGCCGGTCATTCTCGCCTATGCCCGCGGGAACGAGGACGA
GCGGGCTTTCTGGAAAGACGCGATCGGTGGCCACCGGGCGTCCGACGCGGACCTTGCACACGCCATCACGCTTATCGGTC
GGCACAATGCCCTAGAGGACACGCGCACGCGCGCCCGCCACTTCGCCCGGCGAGCGATCGATGCGATCTCGATCTTCCCG
GACAGCAAGGCCCGGCAGGCAATGGCGGAAGCGGCCCTGTTCGCTGTCGCTCGCGGCTATTGA

Upstream 100 bases:

>100_bases
GCGTGTCTCCCGTCCGGTCGGTCGATTCATTCATGCACGAAAGGCTAGCACCAAATGACCGCTTGCCAAGTCAATGAGCG
CACAGCATGGGAAACCAGCG

Downstream 100 bases:

>100_bases
GCGAGGCTGCCGACACCGATCTTCCCATCCGCGCTGCCCTGCCGGCATTGCGGGATGCGTTGGCGCGGTCGAATTCCGCC
GTGCTGGTCGCACCCCCCGG

Product: geranylgeranyl pyrophosphate synthase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MTADIIPIRSNGDDAPPTIEPMLQLTASGMNAVNTVILDRMQSEIPLIPRLAGHLISGGGKRLRPMLTLAGAELVGYQGS
RHHKLAAAVEFIHTATLLHDDVVDGSEMRRGKAAANIIFGNPATVLVGDFLFSRAFELMTEDGSLRVLKILSNASAVIAE
GEVAQLSAQRKLETSEERYLDIIGAKTAALFAAASRISAVVAECDDSHERALDDYGRNLGVAFQLVDDAIDYDSDAAEMG
KDQGDDFREGKMTLPVILAYARGNEDERAFWKDAIGGHRASDADLAHAITLIGRHNALEDTRTRARHFARRAIDAISIFP
DSKARQAMAEAALFAVARGY

Sequences:

>Translated_340_residues
MTADIIPIRSNGDDAPPTIEPMLQLTASGMNAVNTVILDRMQSEIPLIPRLAGHLISGGGKRLRPMLTLAGAELVGYQGS
RHHKLAAAVEFIHTATLLHDDVVDGSEMRRGKAAANIIFGNPATVLVGDFLFSRAFELMTEDGSLRVLKILSNASAVIAE
GEVAQLSAQRKLETSEERYLDIIGAKTAALFAAASRISAVVAECDDSHERALDDYGRNLGVAFQLVDDAIDYDSDAAEMG
KDQGDDFREGKMTLPVILAYARGNEDERAFWKDAIGGHRASDADLAHAITLIGRHNALEDTRTRARHFARRAIDAISIFP
DSKARQAMAEAALFAVARGY
>Mature_339_residues
TADIIPIRSNGDDAPPTIEPMLQLTASGMNAVNTVILDRMQSEIPLIPRLAGHLISGGGKRLRPMLTLAGAELVGYQGSR
HHKLAAAVEFIHTATLLHDDVVDGSEMRRGKAAANIIFGNPATVLVGDFLFSRAFELMTEDGSLRVLKILSNASAVIAEG
EVAQLSAQRKLETSEERYLDIIGAKTAALFAAASRISAVVAECDDSHERALDDYGRNLGVAFQLVDDAIDYDSDAAEMGK
DQGDDFREGKMTLPVILAYARGNEDERAFWKDAIGGHRASDADLAHAITLIGRHNALEDTRTRARHFARRAIDAISIFPD
SKARQAMAEAALFAVARGY

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=298, Percent_Identity=31.2080536912752, Blast_Score=128, Evalue=1e-29,
Organism=Escherichia coli, GI1789578, Length=323, Percent_Identity=47.3684210526316, Blast_Score=291, Evalue=4e-80,
Organism=Escherichia coli, GI1786623, Length=259, Percent_Identity=32.8185328185328, Blast_Score=92, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17505681, Length=303, Percent_Identity=29.7029702970297, Blast_Score=138, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6319475, Length=293, Percent_Identity=30.0341296928328, Blast_Score=127, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6325188, Length=224, Percent_Identity=24.1071428571429, Blast_Score=83, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24651612, Length=302, Percent_Identity=31.4569536423841, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI281365769, Length=266, Percent_Identity=24.812030075188, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24660002, Length=266, Percent_Identity=24.812030075188, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 36693; Mature: 36562

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTADIIPIRSNGDDAPPTIEPMLQLTASGMNAVNTVILDRMQSEIPLIPRLAGHLISGGG
CCCCEEEECCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCC
KRLRPMLTLAGAELVGYQGSRHHKLAAAVEFIHTATLLHDDVVDGSEMRRGKAAANIIFG
HHHHHHHHHHCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHEEEEEC
NPATVLVGDFLFSRAFELMTEDGSLRVLKILSNASAVIAEGEVAQLSAQRKLETSEERYL
CCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEECCHHHHHHHHHHCCCCHHHHH
DIIGAKTAALFAAASRISAVVAECDDSHERALDDYGRNLGVAFQLVDDAIDYDSDAAEMG
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHEEHHHHHCCCCCHHHCC
KDQGDDFREGKMTLPVILAYARGNEDERAFWKDAIGGHRASDADLAHAITLIGRHNALED
CCCCCCHHCCCEEEEHEEEECCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHH
TRTRARHFARRAIDAISIFPDSKARQAMAEAALFAVARGY
HHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TADIIPIRSNGDDAPPTIEPMLQLTASGMNAVNTVILDRMQSEIPLIPRLAGHLISGGG
CCCEEEECCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCC
KRLRPMLTLAGAELVGYQGSRHHKLAAAVEFIHTATLLHDDVVDGSEMRRGKAAANIIFG
HHHHHHHHHHCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHEEEEEC
NPATVLVGDFLFSRAFELMTEDGSLRVLKILSNASAVIAEGEVAQLSAQRKLETSEERYL
CCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEECCHHHHHHHHHHCCCCHHHHH
DIIGAKTAALFAAASRISAVVAECDDSHERALDDYGRNLGVAFQLVDDAIDYDSDAAEMG
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHEEHHHHHCCCCCHHHCC
KDQGDDFREGKMTLPVILAYARGNEDERAFWKDAIGGHRASDADLAHAITLIGRHNALED
CCCCCCHHCCCEEEEHEEEECCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHH
TRTRARHFARRAIDAISIFPDSKARQAMAEAALFAVARGY
HHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]