Click here to switch to the map view.

The map label for this gene is phoQ [H]

Identifier: 85373557

GI number: 85373557

Start: 753451

End: 754947

Strand: Direct

Name: phoQ [H]

Synonym: ELI_03650

Alternate gene names: 85373557

Gene position: 753451-754947 (Clockwise)

Preceding gene: 85373556

Following gene: 85373560

Centisome position: 24.68

GC content: 64.73

Gene sequence:

>1497_bases
GTGGACTCGGCTACAGCCTCGACGACCCGGCCGACGCGCCGCGGGCCTGACGACGCGTCCGATGCCGCACTCGAAGAGGG
CGCGGTGACTGGCGCGCCGGATACGGGCGACGCCGCGCATCCGCTGACGCCAGAGGAGCAGGCGGAGGCTCCACACACCG
GCAGTCTTGCGCGCCGGATGATGCTGATTTCGGCGGCATGGATCCTGGTACTGCTGCTTGGCGGAGGCATCGCCCTCGAT
CGCACCCTCACCAATCTGGTCGAGCGCAATTTCGACGAGCAGCTCGAATATGTGCAAACGGCGCTGCTCTCATCGGCAGA
GATCGGCCCGGATGGAGAAGTTCTTTTGTATCGTGCGCTGGGCGACCAGCGCTTTCTCGAGCCAAACAGCGGCGTCTATT
GGCAGATCAGCGGGGAGGGGCACGAACCCTTCCCGAGTCGCAGCCTGTGGGATCGCAGCCTGCAGGTGCAGGGCGATCAC
ATCGACGACGATCCCCACTATTACGACAGCAACCAGTTTGCCGACGAGCCGCTGAGGGTTGTCGAACGCTCGGTGATCCT
GCCGGGAAGCGACACACGGTGGACCTTTACCGTCGCCTCTGCGCGCGACGAACTCGACGCGCAGATCACGCGAATTCGTT
CGATATTGTTCTGGAGCTTTGTCGTGCTGGCCGTCGGCCTGCTCGTGCTGGCGGCGCTGCAAAGCTATTACGGTTTGCGT
CCGCTGCGGCGAGTACGGCTGGCCATCCAGCGCATCCGTTCGACCGGGCGCAACCGTGTGACCGATCCGCTGCCGCTGGA
AGTCCAGCCGCTGGTCGAAGAACTCAACGCATTGCTGGAGCATTCGGAGAAACAGGCGGAGGAGGCGCGGACCCATGCCG
GCAACCTCGCCCATGCGCTCAAGACCCCGCTGACGGTGCTCACCAACGCCGCCACGGCCCAATCGCCCGATCTCAGTACC
GCCGTGATGCGCGAGACGCGCACGATGCAGCGCCATGTCGATCACCACCTGGCGCGCGCCCGCGCGGTCGGGCGGCGGGC
GGTCGGCCATGCGCGCACATCGGTGTGGCAGAGCGCGCAAGCGGTGCAGCGTGCGGTCGAGCGGCTTTATCCGGACAGCC
GTTTCGACATCGACGGCAACAAGGAAGTCGAGGTCGCGATAGAGCGGCAGGATCTCGACGAAATACTCGGCAATCTCATC
GAGAATGCAGCCAAGTACGGCGGCGGTACGGTATTCGTCACGATCGATGCCGAACCTGCGGTCGAAGCGTGCGTCATCTG
GGTCGAAGACGATGGAACAGGCATTCCCGAAGACGAGCGCGATCGCATCTTCGATCGCGGTGTGCGGCTCGACACGGGCA
AGCCGGGTACCGGCCTCGGCCTCGCTATCGTGCGCGATGTTGCAGAGATTTACGGCGGCGGTGTCGATCTGGCGGAAAGC
GAAGACCTGGGCGGGCTGCTCGTGCGATTATCTCTCCCGCGCGCGGGCCAAGTTTAG

Upstream 100 bases:

>100_bases
CATATCTACGATCAGGATTTCGACCGCGATTCCAATACGATCGAGGTTTTTGTAACGCGCATCCGCAAGAAATTGGGCGC
AGAAGTGATTACGACGATCC

Downstream 100 bases:

>100_bases
GATTTTCAGACGAACAAAGAAAAGGGGCCGGAAAACCGGCCCCTTTGAACTTCGAGTTAGCCTCGCTTACGGGCTGGTCG
GACGTTCGTCGCCAGCAGCA

Product: two-component signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MDSATASTTRPTRRGPDDASDAALEEGAVTGAPDTGDAAHPLTPEEQAEAPHTGSLARRMMLISAAWILVLLLGGGIALD
RTLTNLVERNFDEQLEYVQTALLSSAEIGPDGEVLLYRALGDQRFLEPNSGVYWQISGEGHEPFPSRSLWDRSLQVQGDH
IDDDPHYYDSNQFADEPLRVVERSVILPGSDTRWTFTVASARDELDAQITRIRSILFWSFVVLAVGLLVLAALQSYYGLR
PLRRVRLAIQRIRSTGRNRVTDPLPLEVQPLVEELNALLEHSEKQAEEARTHAGNLAHALKTPLTVLTNAATAQSPDLST
AVMRETRTMQRHVDHHLARARAVGRRAVGHARTSVWQSAQAVQRAVERLYPDSRFDIDGNKEVEVAIERQDLDEILGNLI
ENAAKYGGGTVFVTIDAEPAVEACVIWVEDDGTGIPEDERDRIFDRGVRLDTGKPGTGLGLAIVRDVAEIYGGGVDLAES
EDLGGLLVRLSLPRAGQV

Sequences:

>Translated_498_residues
MDSATASTTRPTRRGPDDASDAALEEGAVTGAPDTGDAAHPLTPEEQAEAPHTGSLARRMMLISAAWILVLLLGGGIALD
RTLTNLVERNFDEQLEYVQTALLSSAEIGPDGEVLLYRALGDQRFLEPNSGVYWQISGEGHEPFPSRSLWDRSLQVQGDH
IDDDPHYYDSNQFADEPLRVVERSVILPGSDTRWTFTVASARDELDAQITRIRSILFWSFVVLAVGLLVLAALQSYYGLR
PLRRVRLAIQRIRSTGRNRVTDPLPLEVQPLVEELNALLEHSEKQAEEARTHAGNLAHALKTPLTVLTNAATAQSPDLST
AVMRETRTMQRHVDHHLARARAVGRRAVGHARTSVWQSAQAVQRAVERLYPDSRFDIDGNKEVEVAIERQDLDEILGNLI
ENAAKYGGGTVFVTIDAEPAVEACVIWVEDDGTGIPEDERDRIFDRGVRLDTGKPGTGLGLAIVRDVAEIYGGGVDLAES
EDLGGLLVRLSLPRAGQV
>Mature_498_residues
MDSATASTTRPTRRGPDDASDAALEEGAVTGAPDTGDAAHPLTPEEQAEAPHTGSLARRMMLISAAWILVLLLGGGIALD
RTLTNLVERNFDEQLEYVQTALLSSAEIGPDGEVLLYRALGDQRFLEPNSGVYWQISGEGHEPFPSRSLWDRSLQVQGDH
IDDDPHYYDSNQFADEPLRVVERSVILPGSDTRWTFTVASARDELDAQITRIRSILFWSFVVLAVGLLVLAALQSYYGLR
PLRRVRLAIQRIRSTGRNRVTDPLPLEVQPLVEELNALLEHSEKQAEEARTHAGNLAHALKTPLTVLTNAATAQSPDLST
AVMRETRTMQRHVDHHLARARAVGRRAVGHARTSVWQSAQAVQRAVERLYPDSRFDIDGNKEVEVAIERQDLDEILGNLI
ENAAKYGGGTVFVTIDAEPAVEACVIWVEDDGTGIPEDERDRIFDRGVRLDTGKPGTGLGLAIVRDVAEIYGGGVDLAES
EDLGGLLVRLSLPRAGQV

Specific function: Member of the two-component regulatory system phoQ/phoP involved in virulence, adaptation to low Mg(2+) environments and the control of acid resistance genes. In presence of low periplasmic Mg(2+) concentrations, phoQ functions as a membrane- associated p

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787374, Length=279, Percent_Identity=31.1827956989247, Blast_Score=125, Evalue=9e-30,
Organism=Escherichia coli, GI1790861, Length=302, Percent_Identity=29.1390728476821, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1786912, Length=258, Percent_Identity=29.8449612403101, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1786783, Length=318, Percent_Identity=25.4716981132075, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1789808, Length=238, Percent_Identity=27.7310924369748, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787894, Length=238, Percent_Identity=29.4117647058824, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR015014
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF08918 PhoQ_Sensor [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 54526; Mature: 54526

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSATASTTRPTRRGPDDASDAALEEGAVTGAPDTGDAAHPLTPEEQAEAPHTGSLARRM
CCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCHHHCCCCCHHHHHHHH
MLISAAWILVLLLGGGIALDRTLTNLVERNFDEQLEYVQTALLSSAEIGPDGEVLLYRAL
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEC
GDQRFLEPNSGVYWQISGEGHEPFPSRSLWDRSLQVQGDHIDDDPHYYDSNQFADEPLRV
CCCCCCCCCCCEEEEEECCCCCCCCCCHHHCCCCEEECCCCCCCCCCCCCCCCCHHHHHH
VERSVILPGSDTRWTFTVASARDELDAQITRIRSILFWSFVVLAVGLLVLAALQSYYGLR
HHHHEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PLRRVRLAIQRIRSTGRNRVTDPLPLEVQPLVEELNALLEHSEKQAEEARTHAGNLAHAL
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KTPLTVLTNAATAQSPDLSTAVMRETRTMQRHVDHHLARARAVGRRAVGHARTSVWQSAQ
HCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVQRAVERLYPDSRFDIDGNKEVEVAIERQDLDEILGNLIENAAKYGGGTVFVTIDAEPA
HHHHHHHHHCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCC
VEACVIWVEDDGTGIPEDERDRIFDRGVRLDTGKPGTGLGLAIVRDVAEIYGGGVDLAES
CEEEEEEEECCCCCCCCHHHHHHHHCCCEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
EDLGGLLVRLSLPRAGQV
CCCCCEEEEEECCCCCCC
>Mature Secondary Structure
MDSATASTTRPTRRGPDDASDAALEEGAVTGAPDTGDAAHPLTPEEQAEAPHTGSLARRM
CCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCHHHCCCCCHHHHHHHH
MLISAAWILVLLLGGGIALDRTLTNLVERNFDEQLEYVQTALLSSAEIGPDGEVLLYRAL
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEC
GDQRFLEPNSGVYWQISGEGHEPFPSRSLWDRSLQVQGDHIDDDPHYYDSNQFADEPLRV
CCCCCCCCCCCEEEEEECCCCCCCCCCHHHCCCCEEECCCCCCCCCCCCCCCCCHHHHHH
VERSVILPGSDTRWTFTVASARDELDAQITRIRSILFWSFVVLAVGLLVLAALQSYYGLR
HHHHEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PLRRVRLAIQRIRSTGRNRVTDPLPLEVQPLVEELNALLEHSEKQAEEARTHAGNLAHAL
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KTPLTVLTNAATAQSPDLSTAVMRETRTMQRHVDHHLARARAVGRRAVGHARTSVWQSAQ
HCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVQRAVERLYPDSRFDIDGNKEVEVAIERQDLDEILGNLIENAAKYGGGTVFVTIDAEPA
HHHHHHHHHCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCC
VEACVIWVEDDGTGIPEDERDRIFDRGVRLDTGKPGTGLGLAIVRDVAEIYGGGVDLAES
CEEEEEEEECCCCCCCCHHHHHHHHCCCEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
EDLGGLLVRLSLPRAGQV
CCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]