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The map label for this gene is treC [C]

Identifier: 85373456

GI number: 85373456

Start: 655809

End: 657212

Strand: Reverse

Name: treC [C]

Synonym: ELI_03145

Alternate gene names: 85373456

Gene position: 657212-655809 (Counterclockwise)

Preceding gene: 85373457

Following gene: 85373455

Centisome position: 21.53

GC content: 63.18

Gene sequence:

>1404_bases
ATGCGTAAATACGCCCTTCCCGCCCTCGCTGCGCTGCTTGTCGGCGGAGCGGCTTTCGCGATGACCGAGGGCGTGTACGA
GCCGGAAGCGAATGCGTGGGAGCCGCAACAATATGTCGAGCTGACCAATCCCGAATGGTCGAAAGATGCGGTTCTCTACC
AGATCAACACGCGCCACTTCACGCCGGAAGGCACCTTCGCCGCAGCGCAGGAAGAGCTGCCGCGTCTGAAGGAACTCGGC
GTCGACATATTGTGGCTGATGCCGATCCATCCGATCGGCGAGGTCAACCGCAAGGGCACGCTGGGCAGCCCCTACTCGGT
CAAGGACTATTACGGGGTGAACCCCGAATTCGGGACCGAGGAAGAGTTTCGCACCTTCGTCGATGCGGCGCACGAGCAAG
GCTTCAAGGTCATCCTCGACCTCGTCGCCAACCACACCGCCTGGGACCACCCGCTCGCCGAGGAACACCCCGACTGGTAC
GAGAAGACCTGGGACGGCGATTTCCGCCCTACGCCTTGGTGGGACTGGTCCGACATCATCGATCTCGACTGGTCCAAGCC
CGGCGTGCGCGAACATGTCGGCAGGGCGATGGAGATGTGGGTCCGGGATTACGGGATCGACGGTTTCCGCGCCGATGTCG
CGGGCTATGTCCCGGTCGATTTCTGGGAGACCATGCGCGCAAGGCTCGATGCCATCCGCCCGGTTTTCATGCTCGGCGAA
GTCCAGCAAAGCGCATGGCACCGCGCCGCGTTCGATGCGACTTATGCATGGGACTGGCACAACACCACCAAGCGCGTTGC
CAAGGGCGATGGCAACGCCACCAGCTTCTACGGCTACTATGCCGAGAACGAGAGCCTGTGGCCGCGCGAGGCCATGCGCA
TGACCTATATCGAGAACCACGATGCCAACGCGTGGGAAGGCACGCTGTACGAAAACTACGGCGACGCGCTGCAGATCATG
ACCGCGCTCAGTTTCACCGGCGAAGGCCTGCCGCTGATCCATAACGGCATGGAAGCCTGCAACGCCAAGCGGCTGGAATT
CTTCGAGCGCGACCCGATCGACTGGGACCAGAACCCTGACTGTACCTATGGTGACCTGCTCAAGGACCTGATCGCCTTCC
GCGATGCCAATCCGGCGCTGCACAACGGCAAATGGGGGGCGCGGATGCACAGGGTCGAAAACAGCGCGCCCGAGCAGCTG
TTCGGCTGGGTGCGCGAGCAGGACGGCAACAAGGTCGTCGGCCTGTTCAATATGAGCACACGGCCGGTGAACGCGACGCT
GAGCGACGGGATCGCAGCGGGCACCTACACGGCATTCCGCGAGGAGGGCGAGGCGACCCTGGCCGAAGGCGACACCGTCA
CCCTCCCGGCCTGGGGCTTCCGCCTGCTGTCGGCAAAGAAGTAG

Upstream 100 bases:

>100_bases
ATCTGGACGATGCTGTTCGCCGCCGCGAGCTGGCTGATCGCCGCTGCCGCGATGCTGCGGGTGCAGGCGGCCCTTCCCTC
CACTTCACCGGAGACTGCCG

Downstream 100 bases:

>100_bases
CGGATATTTGCATGTCGGCCCTGCCGCGCTAACGCTGGCGCGCATGACCGATCACCAACCGCACGTCACCTCCGCCCGTT
CATGGTTGTTCGCCCCCGGC

Product: alpha-amylase, putative

Products: NA

Alternate protein names: CDase; Cyclomaltodextrin hydrolase, decycling [H]

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MRKYALPALAALLVGGAAFAMTEGVYEPEANAWEPQQYVELTNPEWSKDAVLYQINTRHFTPEGTFAAAQEELPRLKELG
VDILWLMPIHPIGEVNRKGTLGSPYSVKDYYGVNPEFGTEEEFRTFVDAAHEQGFKVILDLVANHTAWDHPLAEEHPDWY
EKTWDGDFRPTPWWDWSDIIDLDWSKPGVREHVGRAMEMWVRDYGIDGFRADVAGYVPVDFWETMRARLDAIRPVFMLGE
VQQSAWHRAAFDATYAWDWHNTTKRVAKGDGNATSFYGYYAENESLWPREAMRMTYIENHDANAWEGTLYENYGDALQIM
TALSFTGEGLPLIHNGMEACNAKRLEFFERDPIDWDQNPDCTYGDLLKDLIAFRDANPALHNGKWGARMHRVENSAPEQL
FGWVREQDGNKVVGLFNMSTRPVNATLSDGIAAGTYTAFREEGEATLAEGDTVTLPAWGFRLLSAKK

Sequences:

>Translated_467_residues
MRKYALPALAALLVGGAAFAMTEGVYEPEANAWEPQQYVELTNPEWSKDAVLYQINTRHFTPEGTFAAAQEELPRLKELG
VDILWLMPIHPIGEVNRKGTLGSPYSVKDYYGVNPEFGTEEEFRTFVDAAHEQGFKVILDLVANHTAWDHPLAEEHPDWY
EKTWDGDFRPTPWWDWSDIIDLDWSKPGVREHVGRAMEMWVRDYGIDGFRADVAGYVPVDFWETMRARLDAIRPVFMLGE
VQQSAWHRAAFDATYAWDWHNTTKRVAKGDGNATSFYGYYAENESLWPREAMRMTYIENHDANAWEGTLYENYGDALQIM
TALSFTGEGLPLIHNGMEACNAKRLEFFERDPIDWDQNPDCTYGDLLKDLIAFRDANPALHNGKWGARMHRVENSAPEQL
FGWVREQDGNKVVGLFNMSTRPVNATLSDGIAAGTYTAFREEGEATLAEGDTVTLPAWGFRLLSAKK
>Mature_467_residues
MRKYALPALAALLVGGAAFAMTEGVYEPEANAWEPQQYVELTNPEWSKDAVLYQINTRHFTPEGTFAAAQEELPRLKELG
VDILWLMPIHPIGEVNRKGTLGSPYSVKDYYGVNPEFGTEEEFRTFVDAAHEQGFKVILDLVANHTAWDHPLAEEHPDWY
EKTWDGDFRPTPWWDWSDIIDLDWSKPGVREHVGRAMEMWVRDYGIDGFRADVAGYVPVDFWETMRARLDAIRPVFMLGE
VQQSAWHRAAFDATYAWDWHNTTKRVAKGDGNATSFYGYYAENESLWPREAMRMTYIENHDANAWEGTLYENYGDALQIM
TALSFTGEGLPLIHNGMEACNAKRLEFFERDPIDWDQNPDCTYGDLLKDLIAFRDANPALHNGKWGARMHRVENSAPEQL
FGWVREQDGNKVVGLFNMSTRPVNATLSDGIAAGTYTAFREEGEATLAEGDTVTLPAWGFRLLSAKK

Specific function: Hydrolyzes cyclodextrins. Can also act on linear maltodextrins, with the exception of maltose [H]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1790687, Length=211, Percent_Identity=29.3838862559242, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI2367229, Length=224, Percent_Identity=26.3392857142857, Blast_Score=62, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI32565753, Length=209, Percent_Identity=24.8803827751196, Blast_Score=66, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6321731, Length=215, Percent_Identity=26.9767441860465, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6319776, Length=215, Percent_Identity=26.9767441860465, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6322245, Length=217, Percent_Identity=27.1889400921659, Blast_Score=74, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6322241, Length=215, Percent_Identity=26.046511627907, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6322021, Length=215, Percent_Identity=26.046511627907, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324416, Length=215, Percent_Identity=26.046511627907, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6321726, Length=215, Percent_Identity=26.046511627907, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24586599, Length=223, Percent_Identity=27.3542600896861, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24586591, Length=213, Percent_Identity=32.3943661971831, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24586597, Length=217, Percent_Identity=28.110599078341, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24586587, Length=215, Percent_Identity=27.906976744186, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI45549022, Length=214, Percent_Identity=25.2336448598131, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24586589, Length=212, Percent_Identity=30.6603773584906, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24583745, Length=247, Percent_Identity=26.7206477732794, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24586593, Length=214, Percent_Identity=26.1682242990654, Blast_Score=66, Evalue=4e-11,
Organism=Drosophila melanogaster, GI221330053, Length=214, Percent_Identity=25.7009345794392, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02903 Alpha-amylase_N [H]

EC number: =3.2.1.54 [H]

Molecular weight: Translated: 53170; Mature: 53170

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKYALPALAALLVGGAAFAMTEGVYEPEANAWEPQQYVELTNPEWSKDAVLYQINTRHF
CCCHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCHHHEEECCCCCCCCEEEEEEECEEE
TPEGTFAAAQEELPRLKELGVDILWLMPIHPIGEVNRKGTLGSPYSVKDYYGVNPEFGTE
CCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
EEFRTFVDAAHEQGFKVILDLVANHTAWDHPLAEEHPDWYEKTWDGDFRPTPWWDWSDII
HHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCEE
DLDWSKPGVREHVGRAMEMWVRDYGIDGFRADVAGYVPVDFWETMRARLDAIRPVFMLGE
ECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VQQSAWHRAAFDATYAWDWHNTTKRVAKGDGNATSFYGYYAENESLWPREAMRMTYIENH
HHHHHHHHHHCCCEEEECCCCHHHHHHCCCCCCEEEEEEEECCCCCCHHHHHHHHHEECC
DANAWEGTLYENYGDALQIMTALSFTGEGLPLIHNGMEACNAKRLEFFERDPIDWDQNPD
CCCCCCCCHHHCHHHHHHHHHHHHHCCCCCCHHHCCHHHHCCHHHHHHHCCCCCCCCCCC
CTYGDLLKDLIAFRDANPALHNGKWGARMHRVENSAPEQLFGWVREQDGNKVVGLFNMST
CCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEEECCC
RPVNATLSDGIAAGTYTAFREEGEATLAEGDTVTLPAWGFRLLSAKK
CCCCCHHHCCCCCCHHHHHHHCCCEEEECCCEEEECHHHHHHHCCCC
>Mature Secondary Structure
MRKYALPALAALLVGGAAFAMTEGVYEPEANAWEPQQYVELTNPEWSKDAVLYQINTRHF
CCCHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCHHHEEECCCCCCCCEEEEEEECEEE
TPEGTFAAAQEELPRLKELGVDILWLMPIHPIGEVNRKGTLGSPYSVKDYYGVNPEFGTE
CCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
EEFRTFVDAAHEQGFKVILDLVANHTAWDHPLAEEHPDWYEKTWDGDFRPTPWWDWSDII
HHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCEE
DLDWSKPGVREHVGRAMEMWVRDYGIDGFRADVAGYVPVDFWETMRARLDAIRPVFMLGE
ECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VQQSAWHRAAFDATYAWDWHNTTKRVAKGDGNATSFYGYYAENESLWPREAMRMTYIENH
HHHHHHHHHHCCCEEEECCCCHHHHHHCCCCCCEEEEEEEECCCCCCHHHHHHHHHEECC
DANAWEGTLYENYGDALQIMTALSFTGEGLPLIHNGMEACNAKRLEFFERDPIDWDQNPD
CCCCCCCCHHHCHHHHHHHHHHHHHCCCCCCHHHCCHHHHCCHHHHHHHCCCCCCCCCCC
CTYGDLLKDLIAFRDANPALHNGKWGARMHRVENSAPEQLFGWVREQDGNKVVGLFNMST
CCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEEECCC
RPVNATLSDGIAAGTYTAFREEGEATLAEGDTVTLPAWGFRLLSAKK
CCCCCHHHCCCCCCHHHHHHHCCCEEEECCCEEEECHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7763728 [H]