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The map label for this gene is citE [H]

Identifier: 85373455

GI number: 85373455

Start: 654869

End: 655765

Strand: Reverse

Name: citE [H]

Synonym: ELI_03140

Alternate gene names: 85373455

Gene position: 655765-654869 (Counterclockwise)

Preceding gene: 85373456

Following gene: 85373450

Centisome position: 21.48

GC content: 68.67

Gene sequence:

>897_bases
ATGACCGATCACCAACCGCACGTCACCTCCGCCCGTTCATGGTTGTTCGCCCCCGGCGACAGCGAAAAGAAGATGGACAA
GGCCGCCGCGAGCGAGGCCGATATCGCCATCTTCGACCTGGAAGACGCGGTTGCGCCCGAGGCCAAGCCCGAAGCGCGCA
CCCTGGTCCATGACCGGATCGCGCGCGGGGACGACGACGCGCGCAGGCGGCTGTGGGTGCGGATCAACCCGCTCGACACC
GACATGGCGGCGCAGGACTTGGCCGCAATCATGCCCGCGCGGCCCGGTGGCCTGCTGCTGCCCAAGTCACGCGGGCGCGG
CGACCTCGAGCGGCTGGACGATTTTCTCACCGCGCTGGAAGCCGCCAACGGCATCGCGCTCGGCACTACCCCGGTGCTGG
TGCTCGCGACCGAAACGGCAGGTGCTGTGTTCCATTGCGGCGACTATTCCGGCGCGCCGCGGCTGGCGGGGCTGACCTGG
GGCGCGGAGGACCTGTCCGATGCGCTCGGGGCGAGCGACAATCGCGGGCCGGACGGTACCTACGATCACCCATATATCCT
CGCCCGCAGCCTGTGCCTACTGGGCGCGCGCCATGCCGGGGTGCAACCGGTCGAAACCATCCACGCCGACTTCCGCGATC
TCGACGGCCTCGAAGCGCGCGCCCGCGACGTGAAGCGGCAGGGCTACACCGGCATGCTGGCGATCCACCCGGCGCAGGTC
GAGGTCATCAACCGCGTCTTCACGCCGAGCGAGGAAGAACTCGCCGCCGCGCAAGAGATCGTCGATATCTTCGCCGCCAA
CCCCGGCGCGGGGACGATCGGGCATAACGGCAAGATGCTGGACCGGCCGCATCTGGATAGAGCGAAGGGGTTGTTACGGG
CGGCTGGGCGGATTTAG

Upstream 100 bases:

>100_bases
GGCGACACCGTCACCCTCCCGGCCTGGGGCTTCCGCCTGCTGTCGGCAAAGAAGTAGCGGATATTTGCATGTCGGCCCTG
CCGCGCTAACGCTGGCGCGC

Downstream 100 bases:

>100_bases
CCGCGACCGTCATCCCAGCGAAAGCTGGGATCTCTCTCAATCAAACGAGAGTACCGTAAAGGTCACTCCACTCCGCATTG
GCTTTCTCGATTAGCTCAAC

Product: putative citrate lyase beta chain

Products: NA

Alternate protein names: Citrase beta chain; Citrate (pro-3S)-lyase subunit beta; Citryl-CoA lyase subunit [H]

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MTDHQPHVTSARSWLFAPGDSEKKMDKAAASEADIAIFDLEDAVAPEAKPEARTLVHDRIARGDDDARRRLWVRINPLDT
DMAAQDLAAIMPARPGGLLLPKSRGRGDLERLDDFLTALEAANGIALGTTPVLVLATETAGAVFHCGDYSGAPRLAGLTW
GAEDLSDALGASDNRGPDGTYDHPYILARSLCLLGARHAGVQPVETIHADFRDLDGLEARARDVKRQGYTGMLAIHPAQV
EVINRVFTPSEEELAAAQEIVDIFAANPGAGTIGHNGKMLDRPHLDRAKGLLRAAGRI

Sequences:

>Translated_298_residues
MTDHQPHVTSARSWLFAPGDSEKKMDKAAASEADIAIFDLEDAVAPEAKPEARTLVHDRIARGDDDARRRLWVRINPLDT
DMAAQDLAAIMPARPGGLLLPKSRGRGDLERLDDFLTALEAANGIALGTTPVLVLATETAGAVFHCGDYSGAPRLAGLTW
GAEDLSDALGASDNRGPDGTYDHPYILARSLCLLGARHAGVQPVETIHADFRDLDGLEARARDVKRQGYTGMLAIHPAQV
EVINRVFTPSEEELAAAQEIVDIFAANPGAGTIGHNGKMLDRPHLDRAKGLLRAAGRI
>Mature_297_residues
TDHQPHVTSARSWLFAPGDSEKKMDKAAASEADIAIFDLEDAVAPEAKPEARTLVHDRIARGDDDARRRLWVRINPLDTD
MAAQDLAAIMPARPGGLLLPKSRGRGDLERLDDFLTALEAANGIALGTTPVLVLATETAGAVFHCGDYSGAPRLAGLTWG
AEDLSDALGASDNRGPDGTYDHPYILARSLCLLGARHAGVQPVETIHADFRDLDGLEARARDVKRQGYTGMLAIHPAQVE
VINRVFTPSEEELAAAQEIVDIFAANPGAGTIGHNGKMLDRPHLDRAKGLLRAAGRI

Specific function: Represents a citryl-ACP lyase [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. Citrate lyase beta subunit subfamily [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=304, Percent_Identity=29.9342105263158, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI87081764, Length=297, Percent_Identity=29.6296296296296, Blast_Score=110, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17557458, Length=303, Percent_Identity=26.0726072607261, Blast_Score=99, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR006475
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.3.6; =4.1.3.34 [H]

Molecular weight: Translated: 31924; Mature: 31793

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDHQPHVTSARSWLFAPGDSEKKMDKAAASEADIAIFDLEDAVAPEAKPEARTLVHDRI
CCCCCCCCCCCCCEEECCCCCHHHHHHHHCCCCCEEEEECHHHCCCCCCCHHHHHHHHHH
ARGDDDARRRLWVRINPLDTDMAAQDLAAIMPARPGGLLLPKSRGRGDLERLDDFLTALE
HCCCCHHHEEEEEEECCCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHH
AANGIALGTTPVLVLATETAGAVFHCGDYSGAPRLAGLTWGAEDLSDALGASDNRGPDGT
HCCCEEECCCCEEEEEECCCCEEEECCCCCCCCCCEEEECCHHHHHHHHCCCCCCCCCCC
YDHPYILARSLCLLGARHAGVQPVETIHADFRDLDGLEARARDVKRQGYTGMLAIHPAQV
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEECHHHH
EVINRVFTPSEEELAAAQEIVDIFAANPGAGTIGHNGKMLDRPHLDRAKGLLRAAGRI
HHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TDHQPHVTSARSWLFAPGDSEKKMDKAAASEADIAIFDLEDAVAPEAKPEARTLVHDRI
CCCCCCCCCCCCEEECCCCCHHHHHHHHCCCCCEEEEECHHHCCCCCCCHHHHHHHHHH
ARGDDDARRRLWVRINPLDTDMAAQDLAAIMPARPGGLLLPKSRGRGDLERLDDFLTALE
HCCCCHHHEEEEEEECCCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHH
AANGIALGTTPVLVLATETAGAVFHCGDYSGAPRLAGLTWGAEDLSDALGASDNRGPDGT
HCCCEEECCCCEEEEEECCCCEEEECCCCCCCCCCEEEECCHHHHHHHHCCCCCCCCCCC
YDHPYILARSLCLLGARHAGVQPVETIHADFRDLDGLEARARDVKRQGYTGMLAIHPAQV
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEECHHHH
EVINRVFTPSEEELAAAQEIVDIFAANPGAGTIGHNGKMLDRPHLDRAKGLLRAAGRI
HHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]