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The map label for this gene is pyrE

Identifier: 85373371

GI number: 85373371

Start: 580646

End: 581227

Strand: Reverse

Name: pyrE

Synonym: ELI_02720

Alternate gene names: 85373371

Gene position: 581227-580646 (Counterclockwise)

Preceding gene: 85373387

Following gene: 85373370

Centisome position: 19.04

GC content: 65.64

Gene sequence:

>582_bases
ATGACCGACGAAGAAGTACTGTCCGAATTCCGCGCCAGCGAAGCCCTGCTCGAAGGCCATTTCATGCTCTCCTCGGGCCG
CCACAGCGGGCACTACCTGCAATGTGCGCGGGTGCTGATGAACCCCGAGCGTGCCGGGCGGCTGGCCATGGCACTGGCGC
AGAAACTGCCGCGCGAATTGCGAGGCGAAATCGATGTCGTTGTCTCGCCGGCGATGGGCGGGCTTATTATCGGCCACGAA
ATGGGCCGCGCGCTGGGCAAGGACGCGATCTTCCTCGAACGGCCCGACGGCGAATTCCATTTGCGCCGCGGGTTCCGGCT
GGAGCCGGGGGCCAAGGTTCTGATGGTGGAAGACGTCGTCACCACCGGCCTCTCGAGCCGCGAAGCGATCGAAGCGGTGA
AGCGCGAAGGCGGCGAAGTCGTCGCGGAAGTTTCGCTGGTCGACCGCTCGGCGGGCGAAGCCGACCTTGGCGTGCCCTAC
TATGCGCTGGTGGAGATCAACTTCCCGACCTTTGCCAACGGCGAAGTGCCCGAGGGACTGGCCAGGATCCCCGTCACCAA
GCCGGGCAGCCGGGCGAAGTGA

Upstream 100 bases:

>100_bases
CACAAGCGTCCCTAGGGAGAAAAATACACAAGCCCGGTTCGCTCCTTTGCAACCATCTTGTAACCCGTCCCAACGACCAG
GGAAATACCGCTTTTCTGCC

Downstream 100 bases:

>100_bases
CTCAGCCGCTCCGCCACCATGCTTTGAGATTGGGCGTGAACATCGATCACGTCGCCACCATCCGCAATGCACGCGGCGGG
GACCATCCCGACCCGGTTCG

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase

Number of amino acids: Translated: 193; Mature: 192

Protein sequence:

>193_residues
MTDEEVLSEFRASEALLEGHFMLSSGRHSGHYLQCARVLMNPERAGRLAMALAQKLPRELRGEIDVVVSPAMGGLIIGHE
MGRALGKDAIFLERPDGEFHLRRGFRLEPGAKVLMVEDVVTTGLSSREAIEAVKREGGEVVAEVSLVDRSAGEADLGVPY
YALVEINFPTFANGEVPEGLARIPVTKPGSRAK

Sequences:

>Translated_193_residues
MTDEEVLSEFRASEALLEGHFMLSSGRHSGHYLQCARVLMNPERAGRLAMALAQKLPRELRGEIDVVVSPAMGGLIIGHE
MGRALGKDAIFLERPDGEFHLRRGFRLEPGAKVLMVEDVVTTGLSSREAIEAVKREGGEVVAEVSLVDRSAGEADLGVPY
YALVEINFPTFANGEVPEGLARIPVTKPGSRAK
>Mature_192_residues
TDEEVLSEFRASEALLEGHFMLSSGRHSGHYLQCARVLMNPERAGRLAMALAQKLPRELRGEIDVVVSPAMGGLIIGHEM
GRALGKDAIFLERPDGEFHLRRGFRLEPGAKVLMVEDVVTTGLSSREAIEAVKREGGEVVAEVSLVDRSAGEADLGVPYY
ALVEINFPTFANGEVPEGLARIPVTKPGSRAK

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP)

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRE_ERYLH (Q2NCF5)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_457433.1
- ProteinModelPortal:   Q2NCF5
- SMR:   Q2NCF5
- STRING:   Q2NCF5
- GeneID:   3871772
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_02720
- NMPDR:   fig|314225.3.peg.2539
- eggNOG:   COG0461
- HOGENOM:   HBG404341
- OMA:   FYLQSAK
- ProtClustDB:   PRK00455
- BioCyc:   ELIT314225:ELI_02720-MONOMER
- HAMAP:   MF_01208
- InterPro:   IPR023031
- InterPro:   IPR006273
- InterPro:   IPR000836
- TIGRFAMs:   TIGR01367

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.4.2.10

Molecular weight: Translated: 20921; Mature: 20790

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: BINDING 121-121 BINDING 149-149

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDEEVLSEFRASEALLEGHFMLSSGRHSGHYLQCARVLMNPERAGRLAMALAQKLPREL
CCHHHHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHCCHHH
RGEIDVVVSPAMGGLIIGHEMGRALGKDAIFLERPDGEFHLRRGFRLEPGAKVLMVEDVV
CCCEEEEECCCCCCEEEHHHHHHHHCCCEEEEECCCCCEEECCCCEECCCCEEEEEHHHH
TTGLSSREAIEAVKREGGEVVAEVSLVDRSAGEADLGVPYYALVEINFPTFANGEVPEGL
HCCCCCHHHHHHHHHCCCCEEEEEEEHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCH
ARIPVTKPGSRAK
HCCCCCCCCCCCC
>Mature Secondary Structure 
TDEEVLSEFRASEALLEGHFMLSSGRHSGHYLQCARVLMNPERAGRLAMALAQKLPREL
CHHHHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHCCHHH
RGEIDVVVSPAMGGLIIGHEMGRALGKDAIFLERPDGEFHLRRGFRLEPGAKVLMVEDVV
CCCEEEEECCCCCCEEEHHHHHHHHCCCEEEEECCCCCEEECCCCEECCCCEEEEEHHHH
TTGLSSREAIEAVKREGGEVVAEVSLVDRSAGEADLGVPYYALVEINFPTFANGEVPEGL
HCCCCCHHHHHHHHHCCCCEEEEEEEHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCH
ARIPVTKPGSRAK
HCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA