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The map label for this gene is 85373333

Identifier: 85373333

GI number: 85373333

Start: 548145

End: 549665

Strand: Reverse

Name: 85373333

Synonym: ELI_02530

Alternate gene names: NA

Gene position: 549665-548145 (Counterclockwise)

Preceding gene: 85373334

Following gene: 85373332

Centisome position: 18.01

GC content: 65.61

Gene sequence:

>1521_bases
ATGCCATCATCGCCCACCATCACCCGCCAGCCACCCGTCCGCACGACGCTCGAGCCGTCGAACCATCCCTATCTCTCCGG
CGCGTGGACGCCGTTGCATGAAGAGGTCGATGTCGATGCGCTCGACGTGATCGAGGGCGAAATTCCTGCCGACATCGACG
GCATCTACCTGCGCAATACCGAGAACCAGGTGCACCAGCCGCTGGGGCGGCATCATCCGTTCGACGGGGATGCGATGATC
CACCAGGTCGGCATTTCCGGCGGCATGGCGAGCTATCGCAACCGCTTCGTGCGCACACAGTGTTTCGAGGCGGAGCAGGT
GGCGGGGCGCAGCCTGTGGGGCGGGTTGATGGACCCGGCGGGCACCAGCGAGCGGCCCGGTTTCGGGGCGCATGGCGGCC
TCAAGGATACCGGCAGCACCGACATCATCGTCCATGCCGGTGTCGCCTATGCCACGCTCTATCAATGCGGCGAGGCGTGG
ATGCTCGATCCGGAGACGCTGGAGAATCTCGGCAAGGCGCCATGGGGCCCGCTCGACGGGATTTCCGCGCATCCCAAGGT
CGACGAAGCGACCGGCGAGCTGATGTTCTTCAACTATTCGAAGCACGCGCCCTACATGCATTACGGCGTGGTCGACCGCG
CCGGTCGCCTCGCGCATTACGTGCCGGTGCCGCTGCCCGGTCCGCGCCTGCCGCACGACATGGCGATCACGAAAAACTGG
TCGATCCTCAACGACATGCCGCTGTTCTGGGACGAGGACCTGCTGCAGCGCGATATCCATGCGGCGCGGCTGCACGAGGG
GCTGCCGACGCGCTTTGCGCTGATCCCGCGGCATGGCGGGCCGGAGGACATCCGCTGGTTCGAGGCCGAGCCGACCTTCG
TGCTGCACTGGACCAATGCCTGGGAAGAGACGGCGGAGGACGGCTCCGTCGAAGTGGTGCTGGAGGGCTATCACCAGTCC
AACCCGATGCCCCAGCCGCTCGAGGAATTCGGCCAGTATGCGCATATGATGGCCTATGTCGACGAGCACAGTTTCGAGTG
CCGCCTGCACCGCTGGCGCTTCAACCTTGCAACCGGGGAGACGCGCGAGGAGCGGCTGAACGAACGGATCGTCGAGTTCG
GCACGATCAATCCGCGCTTCGCGATGCGGCCGAGCCGCTATGTCTGGTCGACGACGACCAAGCCGGGCTGGTTCCTGTTC
AACGGCTATGTCCGCCACGACACGCAGACCGGCGAGGAGCAGGTCTATGAACTGCCCGAGGGGGTCTATGCGAGCGAGAG
CCCGATGGTGCCGCGTCAAGGTGGCACGGACGAGGACGATGGGTACTTGGTGACGCTCCTGATCGACGAGAATAGCGGCT
CCAGCGAGTGCGCGATCCTCGATGCCTCGGACATCACCAAGGGGCCTATCTGTCGCCTGGCGCTGCCGCACAAGATCTGC
AGCGGGACGCATTCGACCTGGGTCGAGCGCAGCCAGCTCGAGGCCGACCGCGCCTTCCACCGCGCGCGGTTCGCCGCCTA
G

Upstream 100 bases:

>100_bases
GCGGATGAGAAGAAGTGACGTAGGTAGCGGAGACCTAGCCTCCCTTCCCCTCCCTCCCCCCGCGCGCTACCCTCTTGCCC
GAGAGAGGACCCGCCGCGCC

Downstream 100 bases:

>100_bases
ATTTGCGCGCAAGACTGGCGAAACAGCCCCGCCATTGGCAAGCGCGCAGTTTCGTTTTGCAACCGAGCCGGACCACCCCT
AGGCATGGCGCAAGCAGGAA

Product: hypothetical protein

Products: NA

Alternate protein names: ACO; 8'-apo-beta-carotenal 15,15'-oxygenase; Diox1 [H]

Number of amino acids: Translated: 506; Mature: 505

Protein sequence:

>506_residues
MPSSPTITRQPPVRTTLEPSNHPYLSGAWTPLHEEVDVDALDVIEGEIPADIDGIYLRNTENQVHQPLGRHHPFDGDAMI
HQVGISGGMASYRNRFVRTQCFEAEQVAGRSLWGGLMDPAGTSERPGFGAHGGLKDTGSTDIIVHAGVAYATLYQCGEAW
MLDPETLENLGKAPWGPLDGISAHPKVDEATGELMFFNYSKHAPYMHYGVVDRAGRLAHYVPVPLPGPRLPHDMAITKNW
SILNDMPLFWDEDLLQRDIHAARLHEGLPTRFALIPRHGGPEDIRWFEAEPTFVLHWTNAWEETAEDGSVEVVLEGYHQS
NPMPQPLEEFGQYAHMMAYVDEHSFECRLHRWRFNLATGETREERLNERIVEFGTINPRFAMRPSRYVWSTTTKPGWFLF
NGYVRHDTQTGEEQVYELPEGVYASESPMVPRQGGTDEDDGYLVTLLIDENSGSSECAILDASDITKGPICRLALPHKIC
SGTHSTWVERSQLEADRAFHRARFAA

Sequences:

>Translated_506_residues
MPSSPTITRQPPVRTTLEPSNHPYLSGAWTPLHEEVDVDALDVIEGEIPADIDGIYLRNTENQVHQPLGRHHPFDGDAMI
HQVGISGGMASYRNRFVRTQCFEAEQVAGRSLWGGLMDPAGTSERPGFGAHGGLKDTGSTDIIVHAGVAYATLYQCGEAW
MLDPETLENLGKAPWGPLDGISAHPKVDEATGELMFFNYSKHAPYMHYGVVDRAGRLAHYVPVPLPGPRLPHDMAITKNW
SILNDMPLFWDEDLLQRDIHAARLHEGLPTRFALIPRHGGPEDIRWFEAEPTFVLHWTNAWEETAEDGSVEVVLEGYHQS
NPMPQPLEEFGQYAHMMAYVDEHSFECRLHRWRFNLATGETREERLNERIVEFGTINPRFAMRPSRYVWSTTTKPGWFLF
NGYVRHDTQTGEEQVYELPEGVYASESPMVPRQGGTDEDDGYLVTLLIDENSGSSECAILDASDITKGPICRLALPHKIC
SGTHSTWVERSQLEADRAFHRARFAA
>Mature_505_residues
PSSPTITRQPPVRTTLEPSNHPYLSGAWTPLHEEVDVDALDVIEGEIPADIDGIYLRNTENQVHQPLGRHHPFDGDAMIH
QVGISGGMASYRNRFVRTQCFEAEQVAGRSLWGGLMDPAGTSERPGFGAHGGLKDTGSTDIIVHAGVAYATLYQCGEAWM
LDPETLENLGKAPWGPLDGISAHPKVDEATGELMFFNYSKHAPYMHYGVVDRAGRLAHYVPVPLPGPRLPHDMAITKNWS
ILNDMPLFWDEDLLQRDIHAARLHEGLPTRFALIPRHGGPEDIRWFEAEPTFVLHWTNAWEETAEDGSVEVVLEGYHQSN
PMPQPLEEFGQYAHMMAYVDEHSFECRLHRWRFNLATGETREERLNERIVEFGTINPRFAMRPSRYVWSTTTKPGWFLFN
GYVRHDTQTGEEQVYELPEGVYASESPMVPRQGGTDEDDGYLVTLLIDENSGSSECAILDASDITKGPICRLALPHKICS
GTHSTWVERSQLEADRAFHRARFAA

Specific function: Cleaves a number of carotenals and carotenols in the all-trans configuration at the 15-15' double bond producing retinal or retinol, respectively. Also shows activity toward lycopenals and the corresponding alcohols. Does not cleave beta- carotene or lyco

COG id: COG3670

COG function: function code Q; Lignostilbene-alpha,beta-dioxygenase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carotenoid oxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004294 [H]

Pfam domain/function: PF03055 RPE65 [H]

EC number: =1.14.99.41 [H]

Molecular weight: Translated: 56874; Mature: 56743

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSSPTITRQPPVRTTLEPSNHPYLSGAWTPLHEEVDVDALDVIEGEIPADIDGIYLRNT
CCCCCCCCCCCCCCEECCCCCCCEECCCCCCCCCCCCCCHHHHHCCCCCCCCCCEEEECC
ENQVHQPLGRHHPFDGDAMIHQVGISGGMASYRNRFVRTQCFEAEQVAGRSLWGGLMDPA
CHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCHHHHHCHHHHCCCCCCC
GTSERPGFGAHGGLKDTGSTDIIVHAGVAYATLYQCGEAWMLDPETLENLGKAPWGPLDG
CCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHCCCCEEECHHHHHHHCCCCCCCCCC
ISAHPKVDEATGELMFFNYSKHAPYMHYGVVDRAGRLAHYVPVPLPGPRLPHDMAITKNW
CCCCCCCCCCCCCEEEEECCCCCCEEEECHHHHCCCEEEEECCCCCCCCCCCCEEEECCC
SILNDMPLFWDEDLLQRDIHAARLHEGLPTRFALIPRHGGPEDIRWFEAEPTFVLHWTNA
CHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEECCCCEEEEECCC
WEETAEDGSVEVVLEGYHQSNPMPQPLEEFGQYAHMMAYVDEHSFECRLHRWRFNLATGE
HHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCC
TREERLNERIVEFGTINPRFAMRPSRYVWSTTTKPGWFLFNGYVRHDTQTGEEQVYELPE
HHHHHHHHHHHHHCCCCCCEEECCCCEEEECCCCCCEEEEECEEECCCCCCHHHHHHCCC
GVYASESPMVPRQGGTDEDDGYLVTLLIDENSGSSECAILDASDITKGPICRLALPHKIC
CCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCEEEEECCCCCCCCEEEEECCHHHH
SGTHSTWVERSQLEADRAFHRARFAA
CCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PSSPTITRQPPVRTTLEPSNHPYLSGAWTPLHEEVDVDALDVIEGEIPADIDGIYLRNT
CCCCCCCCCCCCCEECCCCCCCEECCCCCCCCCCCCCCHHHHHCCCCCCCCCCEEEECC
ENQVHQPLGRHHPFDGDAMIHQVGISGGMASYRNRFVRTQCFEAEQVAGRSLWGGLMDPA
CHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCHHHHHCHHHHCCCCCCC
GTSERPGFGAHGGLKDTGSTDIIVHAGVAYATLYQCGEAWMLDPETLENLGKAPWGPLDG
CCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHCCCCEEECHHHHHHHCCCCCCCCCC
ISAHPKVDEATGELMFFNYSKHAPYMHYGVVDRAGRLAHYVPVPLPGPRLPHDMAITKNW
CCCCCCCCCCCCCEEEEECCCCCCEEEECHHHHCCCEEEEECCCCCCCCCCCCEEEECCC
SILNDMPLFWDEDLLQRDIHAARLHEGLPTRFALIPRHGGPEDIRWFEAEPTFVLHWTNA
CHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEECCCCEEEEECCC
WEETAEDGSVEVVLEGYHQSNPMPQPLEEFGQYAHMMAYVDEHSFECRLHRWRFNLATGE
HHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCC
TREERLNERIVEFGTINPRFAMRPSRYVWSTTTKPGWFLFNGYVRHDTQTGEEQVYELPE
HHHHHHHHHHHHHCCCCCCEEECCCCEEEECCCCCCEEEEECEEECCCCCCHHHHHHCCC
GVYASESPMVPRQGGTDEDDGYLVTLLIDENSGSSECAILDASDITKGPICRLALPHKIC
CCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCEEEEECCCCCCCCEEEEECCHHHH
SGTHSTWVERSQLEADRAFHRARFAA
CCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]