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The map label for this gene is gspE [C]

Identifier: 85373310

GI number: 85373310

Start: 519839

End: 521326

Strand: Reverse

Name: gspE [C]

Synonym: ELI_02415

Alternate gene names: 85373310

Gene position: 521326-519839 (Counterclockwise)

Preceding gene: 85373315

Following gene: 85373308

Centisome position: 17.08

GC content: 62.5

Gene sequence:

>1488_bases
ATGGCATCCGGCAGCCGTCCTGCATTCGGCGTCGCGCGCCCCATGAAAGGCGGCGCAAAGGCCAAGGCCGATCCCGGTGG
CGAGCAATTCCCGCCGCTACCGGGCGAAACCGAGGGCAAGGAAGACAGCGCATCGGCACCCACCCCAAGGGCAGGTGCCG
ACGACGCGCTGACGCGGCTGGCCGATCGTGCCAACCATATCCACGAAAAAACGGAAATCGGCGGGTTCGAAGCCAGCGTC
CACAAGATCAAGGAGCAGGTCCTCCCCCGCCTGCTCGAACGTGTCGATCCGGAAGCCGCCGCTACGCTGTCCAAGGAAGA
GCTGTCGGAAGAGTTCCGGCCGATCATCATGGAAGTGCTGGCCGAGCTGAAGGTCACGCTCAACCGCCGCGAGCAATTCG
CGCTCGAGAAGGTCCTGATCGACGAGCTGCTCGGCTTCGGGCCGCTGGAAGAGCTGCTGCAGGATCCCGACGTGTCGGAC
ATCATGGTCAATGGTCCGGACCAGACCTACATCGAGAAAAAGGGCAAGCTGGTGCTCGCCCCGATCCAGTTCCGCGACGA
GAGCCACCTGTTCCAGATCGCACAGCGCATCGTGAACCAGGTCGGCCGCCGCGTCGACCAGACCACGCCTCTGGCAGACG
CCCGCCTCAAGGACGGCAGCCGTGTGAACGTCATCGTGCCGCCGCTTTCGCTGCGCGGTACCGCGATCTCGATCCGTAAA
TTCTCCGAAAAGCCGATTACATTGGACATGCTCAAGGACTTCGGTTCGATGAGCGACAAGATGTGCACCGCACTCAAGAT
CGCGGGCGCCTGCCGCATGAACATCGTCATCTCGGGCGGTACCGGTTCGGGTAAGACGACCATGCTCAACGCCCTGTCGA
AGATGATCGACCCGGGCGAGCGCGTGCTGACGATCGAGGATGCCGCCGAGCTTCGTCTGCAGCAGCCGCACTGGCTGCCG
CTCGAAACGCGTCCGCCGAACCTCGAAGGACAGGGCGCGATCACCATCGGCGATCTCGTGAAGAACGCCCTGCGTATGCG
CCCCGACCGGATCATCCTGGGTGAGATTCGCGGCGCGGAATGTTTCGACCTTCTCGCCGCCATGAACACCGGTCACGACG
GCTCGATGTGTACGCTTCACGCCAACAGCCCGCGCGAGTGCCTCGGTCGTATGGAAAACATGATCCTGATGGGCGACATC
AAGATCCCGAAGGAAGCCATCTCGCGCCAGATCGCCGAATCGGTCGACCTCATCGTGCAGGTCAAGCGCCTCCGCGACGG
TTCGCGTCGCACCACCGACATCACCGAGGTGATCGGGATGGAAGGCGACGTGATCGTGACGCAGAACCTGTTCAAGTTCG
AATACCTGGACGAAAGCGAGGACGGCAAAATCCTCGGCGAATTCCGTTCCTCGGGCCTGCGTCCGTATACGCTCGAGAAA
GCCCGTCAGTTCGGGTTCGACCAGGCTTACTTGGAGGCTTGTCTCTAG

Upstream 100 bases:

>100_bases
AACCTCTTCGTCGTTGGGCGGAGAGAGCGGGTCGCGCGCTGTGATTTATCATAGGGGACAGTGAATGAGTGCTTTTGGAC
GCAAAAACGGGCCTGGTGGC

Downstream 100 bases:

>100_bases
GGTCCTCGTTTTTCGCACCCACATCCGTGCGTGCGTCCTCGCTAGATGTGCGGCAAGCCGCACCCGCTGCGGGCGGGCGG
TCGCCCTTGCGGCCCCTCCG

Product: pilus assembly protein CpaF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MASGSRPAFGVARPMKGGAKAKADPGGEQFPPLPGETEGKEDSASAPTPRAGADDALTRLADRANHIHEKTEIGGFEASV
HKIKEQVLPRLLERVDPEAAATLSKEELSEEFRPIIMEVLAELKVTLNRREQFALEKVLIDELLGFGPLEELLQDPDVSD
IMVNGPDQTYIEKKGKLVLAPIQFRDESHLFQIAQRIVNQVGRRVDQTTPLADARLKDGSRVNVIVPPLSLRGTAISIRK
FSEKPITLDMLKDFGSMSDKMCTALKIAGACRMNIVISGGTGSGKTTMLNALSKMIDPGERVLTIEDAAELRLQQPHWLP
LETRPPNLEGQGAITIGDLVKNALRMRPDRIILGEIRGAECFDLLAAMNTGHDGSMCTLHANSPRECLGRMENMILMGDI
KIPKEAISRQIAESVDLIVQVKRLRDGSRRTTDITEVIGMEGDVIVTQNLFKFEYLDESEDGKILGEFRSSGLRPYTLEK
ARQFGFDQAYLEACL

Sequences:

>Translated_495_residues
MASGSRPAFGVARPMKGGAKAKADPGGEQFPPLPGETEGKEDSASAPTPRAGADDALTRLADRANHIHEKTEIGGFEASV
HKIKEQVLPRLLERVDPEAAATLSKEELSEEFRPIIMEVLAELKVTLNRREQFALEKVLIDELLGFGPLEELLQDPDVSD
IMVNGPDQTYIEKKGKLVLAPIQFRDESHLFQIAQRIVNQVGRRVDQTTPLADARLKDGSRVNVIVPPLSLRGTAISIRK
FSEKPITLDMLKDFGSMSDKMCTALKIAGACRMNIVISGGTGSGKTTMLNALSKMIDPGERVLTIEDAAELRLQQPHWLP
LETRPPNLEGQGAITIGDLVKNALRMRPDRIILGEIRGAECFDLLAAMNTGHDGSMCTLHANSPRECLGRMENMILMGDI
KIPKEAISRQIAESVDLIVQVKRLRDGSRRTTDITEVIGMEGDVIVTQNLFKFEYLDESEDGKILGEFRSSGLRPYTLEK
ARQFGFDQAYLEACL
>Mature_494_residues
ASGSRPAFGVARPMKGGAKAKADPGGEQFPPLPGETEGKEDSASAPTPRAGADDALTRLADRANHIHEKTEIGGFEASVH
KIKEQVLPRLLERVDPEAAATLSKEELSEEFRPIIMEVLAELKVTLNRREQFALEKVLIDELLGFGPLEELLQDPDVSDI
MVNGPDQTYIEKKGKLVLAPIQFRDESHLFQIAQRIVNQVGRRVDQTTPLADARLKDGSRVNVIVPPLSLRGTAISIRKF
SEKPITLDMLKDFGSMSDKMCTALKIAGACRMNIVISGGTGSGKTTMLNALSKMIDPGERVLTIEDAAELRLQQPHWLPL
ETRPPNLEGQGAITIGDLVKNALRMRPDRIILGEIRGAECFDLLAAMNTGHDGSMCTLHANSPRECLGRMENMILMGDIK
IPKEAISRQIAESVDLIVQVKRLRDGSRRTTDITEVIGMEGDVIVTQNLFKFEYLDESEDGKILGEFRSSGLRPYTLEKA
RQFGFDQAYLEACL

Specific function: Involved In A General Secretion Pathway (Gsp) For The Export Of Proteins (By Similarity). [C]

COG id: COG4962

COG function: function code U; Flp pilus assembly protein, ATPase CpaF

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022399
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 54458; Mature: 54327

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00227 TUBULIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASGSRPAFGVARPMKGGAKAKADPGGEQFPPLPGETEGKEDSASAPTPRAGADDALTRL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
ADRANHIHEKTEIGGFEASVHKIKEQVLPRLLERVDPEAAATLSKEELSEEFRPIIMEVL
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
AELKVTLNRREQFALEKVLIDELLGFGPLEELLQDPDVSDIMVNGPDQTYIEKKGKLVLA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCEEEECCCCHHHHHCCCCEEEE
PIQFRDESHLFQIAQRIVNQVGRRVDQTTPLADARLKDGSRVNVIVPPLSLRGTAISIRK
EEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCEEEEEECCCCCCCCEEEEEE
FSEKPITLDMLKDFGSMSDKMCTALKIAGACRMNIVISGGTGSGKTTMLNALSKMIDPGE
CCCCCEEHHHHHHHHCHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCC
RVLTIEDAAELRLQQPHWLPLETRPPNLEGQGAITIGDLVKNALRMRPDRIILGEIRGAE
EEEEEECHHHHHCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHCCCCEEEEECCCCHH
CFDLLAAMNTGHDGSMCTLHANSPRECLGRMENMILMGDIKIPKEAISRQIAESVDLIVQ
HHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHHHHHH
VKRLRDGSRRTTDITEVIGMEGDVIVTQNLFKFEYLDESEDGKILGEFRSSGLRPYTLEK
HHHHCCCCCCCHHHHHHHCCCCCEEEECCCEEEEECCCCCCCCCHHHHHHCCCCCHHHHH
ARQFGFDQAYLEACL
HHHCCCCHHHHHHCC
>Mature Secondary Structure 
ASGSRPAFGVARPMKGGAKAKADPGGEQFPPLPGETEGKEDSASAPTPRAGADDALTRL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
ADRANHIHEKTEIGGFEASVHKIKEQVLPRLLERVDPEAAATLSKEELSEEFRPIIMEVL
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
AELKVTLNRREQFALEKVLIDELLGFGPLEELLQDPDVSDIMVNGPDQTYIEKKGKLVLA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCEEEECCCCHHHHHCCCCEEEE
PIQFRDESHLFQIAQRIVNQVGRRVDQTTPLADARLKDGSRVNVIVPPLSLRGTAISIRK
EEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCEEEEEECCCCCCCCEEEEEE
FSEKPITLDMLKDFGSMSDKMCTALKIAGACRMNIVISGGTGSGKTTMLNALSKMIDPGE
CCCCCEEHHHHHHHHCHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCC
RVLTIEDAAELRLQQPHWLPLETRPPNLEGQGAITIGDLVKNALRMRPDRIILGEIRGAE
EEEEEECHHHHHCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHCCCCEEEEECCCCHH
CFDLLAAMNTGHDGSMCTLHANSPRECLGRMENMILMGDIKIPKEAISRQIAESVDLIVQ
HHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHHHHHH
VKRLRDGSRRTTDITEVIGMEGDVIVTQNLFKFEYLDESEDGKILGEFRSSGLRPYTLEK
HHHHCCCCCCCHHHHHHHCCCCCEEEECCCEEEEECCCCCCCCCHHHHHHCCCCCHHHHH
ARQFGFDQAYLEACL
HHHCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218036; 9634230 [H]