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The map label for this gene is rimO

Identifier: 85372941

GI number: 85372941

Start: 148572

End: 149960

Strand: Reverse

Name: rimO

Synonym: ELI_00570

Alternate gene names: 85372941

Gene position: 149960-148572 (Counterclockwise)

Preceding gene: 85372946

Following gene: 85372940

Centisome position: 4.91

GC content: 66.02

Gene sequence:

>1389_bases
ATGAGCACAATCACTTCCGCGATCCCCGACCAGAAGAAGGTCGGCATGGTTTCCCTCGGCTGCCCCAAGGCGCTGGTCGA
TTCCGAGCGCATCCTCACGCGGCTGCGCGCCGATGGCTACGCCATGAGCGCGGACTACGCCGGGGCCGACGTGGTGCTGG
TCAATACCTGCGGATTCCTTGATTCCGCCAAGGAGGAGAGCCTGGCCGCGATCGGCGAGGCCATTGCCGAGAACGGCCGC
GTGATCGTGACCGGCTGCATGGGCGAGGAAGCCGATGCGATCCGTGCCGCGCACCCGCAGGTGCTGGCGGTGACCGGGGC
GCATCAATACGAGGCGGTGGTCGGGGCGGTGCACGAACATGCCCCGCCGAGCCAGGGTCCATACGTCGATCTGATCCCGC
AGCCGTCAGAGGCCGACATCAAGCTGACGCCGCGGCACTACAGCTATCTCAAGATTTCCGAGGGCTGCAATCACTCCTGC
GCCTTCTGCATCATCCCGCAATTGCGCGGGAAACTGGCCAGCCGACGGATCGACGCAGTGCTGCGCGAGGCGGAGAAACT
GGTCGCGGCGGGTACGAAGGAATTACTGGTGATCAGCCAGGACACCTCGGCCTATGGCGTCGATACCCGGCACGAGGCGA
AGTCCTGGAAAGGTCGCGAGGTCCGAGCGCACATGACCGACCTCGCCCGCGAGCTGGGCCAGCTCGACACCGGAGACGGA
ACCCCGCCCTGGGTCCGGCTGCACTACGTCTACCCCTACCCCCATGTCGATGCGGTGATCCCGCTGATGGCGGAGGGGCT
GCTGACGCCTTATCTCGACATTCCTTTCCAGCATGCCAGCCCTAAAGTGCTGAAGGCGATGAAGCGCCCGGCCAATGAAG
CCAAGGTGCTCGAGAGGCTCAAGGGCTGGCGCGCAATCTGTCCCGAGATCGCCGTGCGTTCGAGCTTCGTCGTCGGCTTC
CCCGGCGAGACCGAGGACGATTTCCGGTACCTGCTCGAATGGCTGGAAGAAGCCCGGCTCGACCGCGTCGGCGCATTCCG
TTTCGAACCGGTCGAAGGTGCCGCGGCCAATGCGCTGCCCGATCCGGTGCCCGAAGAGGTCAAGGAAGAGCGGTACGCCC
GCATAATGGAACTGACCGCCCGCATCAGCGCGGAGAAGCTCCAGCGCAAAATCGGCCGGGCCCTGCCAGTCATCATCGAC
GAGGTCGGCGAGCCGGACGAGGACGGCGACATCGGTGCCACCGGCCGCAGCCAGGCCGACGCGCCGGAGATCGACGGTGC
GGTCTACCTGCGCAATGTCCCCGCCACGCTGGCCGCCGGCGACATCGTGCAGGTAACGGTCGAGGATGCCGACGAGCACG
ACCTGTTCGGCGCGATCGCGCGAGTCTGA

Upstream 100 bases:

>100_bases
AGCCAGTTCACATCCAGCAAGTTCATAATAATCCCCTCCCTCGACCCTCGATCGAGGGGATAGCGCATTTGCGCCATCAG
GTGTAGAGGGCCGCGCAATC

Downstream 100 bases:

>100_bases
TAGAACGGGAACATCGGGGCGACAAAGGTGGCAGCAGATGTGCACCCCGTGCGACGCTGTCGTTTATGAATTTCGCGGCA
GCGCATGAACGCAAGCATGG

Product: ribosomal protein S12 methylthiotransferase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MSTITSAIPDQKKVGMVSLGCPKALVDSERILTRLRADGYAMSADYAGADVVLVNTCGFLDSAKEESLAAIGEAIAENGR
VIVTGCMGEEADAIRAAHPQVLAVTGAHQYEAVVGAVHEHAPPSQGPYVDLIPQPSEADIKLTPRHYSYLKISEGCNHSC
AFCIIPQLRGKLASRRIDAVLREAEKLVAAGTKELLVISQDTSAYGVDTRHEAKSWKGREVRAHMTDLARELGQLDTGDG
TPPWVRLHYVYPYPHVDAVIPLMAEGLLTPYLDIPFQHASPKVLKAMKRPANEAKVLERLKGWRAICPEIAVRSSFVVGF
PGETEDDFRYLLEWLEEARLDRVGAFRFEPVEGAAANALPDPVPEEVKEERYARIMELTARISAEKLQRKIGRALPVIID
EVGEPDEDGDIGATGRSQADAPEIDGAVYLRNVPATLAAGDIVQVTVEDADEHDLFGAIARV

Sequences:

>Translated_462_residues
MSTITSAIPDQKKVGMVSLGCPKALVDSERILTRLRADGYAMSADYAGADVVLVNTCGFLDSAKEESLAAIGEAIAENGR
VIVTGCMGEEADAIRAAHPQVLAVTGAHQYEAVVGAVHEHAPPSQGPYVDLIPQPSEADIKLTPRHYSYLKISEGCNHSC
AFCIIPQLRGKLASRRIDAVLREAEKLVAAGTKELLVISQDTSAYGVDTRHEAKSWKGREVRAHMTDLARELGQLDTGDG
TPPWVRLHYVYPYPHVDAVIPLMAEGLLTPYLDIPFQHASPKVLKAMKRPANEAKVLERLKGWRAICPEIAVRSSFVVGF
PGETEDDFRYLLEWLEEARLDRVGAFRFEPVEGAAANALPDPVPEEVKEERYARIMELTARISAEKLQRKIGRALPVIID
EVGEPDEDGDIGATGRSQADAPEIDGAVYLRNVPATLAAGDIVQVTVEDADEHDLFGAIARV
>Mature_461_residues
STITSAIPDQKKVGMVSLGCPKALVDSERILTRLRADGYAMSADYAGADVVLVNTCGFLDSAKEESLAAIGEAIAENGRV
IVTGCMGEEADAIRAAHPQVLAVTGAHQYEAVVGAVHEHAPPSQGPYVDLIPQPSEADIKLTPRHYSYLKISEGCNHSCA
FCIIPQLRGKLASRRIDAVLREAEKLVAAGTKELLVISQDTSAYGVDTRHEAKSWKGREVRAHMTDLARELGQLDTGDGT
PPWVRLHYVYPYPHVDAVIPLMAEGLLTPYLDIPFQHASPKVLKAMKRPANEAKVLERLKGWRAICPEIAVRSSFVVGFP
GETEDDFRYLLEWLEEARLDRVGAFRFEPVEGAAANALPDPVPEEVKEERYARIMELTARISAEKLQRKIGRALPVIIDE
VGEPDEDGDIGATGRSQADAPEIDGAVYLRNVPATLAAGDIVQVTVEDADEHDLFGAIARV

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI93277076, Length=409, Percent_Identity=26.4058679706601, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI28872784, Length=428, Percent_Identity=25.2336448598131, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI28872782, Length=428, Percent_Identity=25.2336448598131, Blast_Score=122, Evalue=7e-28,
Organism=Escherichia coli, GI1787057, Length=454, Percent_Identity=60.7929515418502, Blast_Score=557, Evalue=1e-160,
Organism=Escherichia coli, GI1786882, Length=469, Percent_Identity=25.3731343283582, Blast_Score=137, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17553146, Length=474, Percent_Identity=24.2616033755274, Blast_Score=111, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI71996771, Length=262, Percent_Identity=25.1908396946565, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21356207, Length=491, Percent_Identity=23.8289205702648, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI19922432, Length=411, Percent_Identity=25.3041362530414, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_ERYLH (Q2NDN5)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_457003.1
- STRING:   Q2NDN5
- GeneID:   3870991
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_00570
- NMPDR:   fig|314225.3.peg.2978
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- PhylomeDB:   Q2NDN5
- ProtClustDB:   PRK14862
- BioCyc:   ELIT314225:ELI_00570-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 50226; Mature: 50094

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTITSAIPDQKKVGMVSLGCPKALVDSERILTRLRADGYAMSADYAGADVVLVNTCGFL
CCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEECCCC
DSAKEESLAAIGEAIAENGRVIVTGCMGEEADAIRAAHPQVLAVTGAHQYEAVVGAVHEH
CCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHCCCCEEEEECCHHHHHHHHHHHHC
APPSQGPYVDLIPQPSEADIKLTPRHYSYLKISEGCNHSCAFCIIPQLRGKLASRRIDAV
CCCCCCCEEEECCCCCCCCEEECCCCCEEEEECCCCCCCEEEEEEHHHHHHHHHHHHHHH
LREAEKLVAAGTKELLVISQDTSAYGVDTRHEAKSWKGREVRAHMTDLARELGQLDTGDG
HHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHCCCCCCCC
TPPWVRLHYVYPYPHVDAVIPLMAEGLLTPYLDIPFQHASPKVLKAMKRPANEAKVLERL
CCCEEEEEEECCCCCHHHHHHHHHHHHCCCHHCCCCCCCCHHHHHHHHCCCHHHHHHHHH
KGWRAICPEIAVRSSFVVGFPGETEDDFRYLLEWLEEARLDRVGAFRFEPVEGAAANALP
HHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCC
DPVPEEVKEERYARIMELTARISAEKLQRKIGRALPVIIDEVGEPDEDGDIGATGRSQAD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCC
APEIDGAVYLRNVPATLAAGDIVQVTVEDADEHDLFGAIARV
CCCCCCEEEEECCCCHHCCCCEEEEEECCCCCHHHHHHHHCC
>Mature Secondary Structure 
STITSAIPDQKKVGMVSLGCPKALVDSERILTRLRADGYAMSADYAGADVVLVNTCGFL
CCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEECCCC
DSAKEESLAAIGEAIAENGRVIVTGCMGEEADAIRAAHPQVLAVTGAHQYEAVVGAVHEH
CCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHCCCCEEEEECCHHHHHHHHHHHHC
APPSQGPYVDLIPQPSEADIKLTPRHYSYLKISEGCNHSCAFCIIPQLRGKLASRRIDAV
CCCCCCCEEEECCCCCCCCEEECCCCCEEEEECCCCCCCEEEEEEHHHHHHHHHHHHHHH
LREAEKLVAAGTKELLVISQDTSAYGVDTRHEAKSWKGREVRAHMTDLARELGQLDTGDG
HHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHCCCCCCCC
TPPWVRLHYVYPYPHVDAVIPLMAEGLLTPYLDIPFQHASPKVLKAMKRPANEAKVLERL
CCCEEEEEEECCCCCHHHHHHHHHHHHCCCHHCCCCCCCCHHHHHHHHCCCHHHHHHHHH
KGWRAICPEIAVRSSFVVGFPGETEDDFRYLLEWLEEARLDRVGAFRFEPVEGAAANALP
HHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCC
DPVPEEVKEERYARIMELTARISAEKLQRKIGRALPVIIDEVGEPDEDGDIGATGRSQAD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCC
APEIDGAVYLRNVPATLAAGDIVQVTVEDADEHDLFGAIARV
CCCCCCEEEEECCCCHHCCCCEEEEEECCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA