Click here to switch to the map view.

The map label for this gene is sigK [H]

Identifier: 85372940

GI number: 85372940

Start: 147836

End: 148387

Strand: Reverse

Name: sigK [H]

Synonym: ELI_00565

Alternate gene names: 85372940

Gene position: 148387-147836 (Counterclockwise)

Preceding gene: 85372941

Following gene: 85372939

Centisome position: 4.86

GC content: 65.76

Gene sequence:

>552_bases
ATGACACAATCACGCCGCGAGGCGCTCAAACTGGCGATGGCAGGCATGGCGGCGGGCGACCGCGCCTCGCTCGATACGGT
CTATGAAATGACATCGTCGAAACTCTTCGCCTCCATCCTGCGCGTCGTCCGCGTCCGTGAGCGGGCCGAGGACCTGCTGC
AGGACACTTATGTCAAGGCATGGCAGCGGGCCGGCCGGTTCGATCCCGCCAAGGGCAGCCCGATCACCTGGCTGTGCACG
ATCGCGCGCAACACCGCGCTCAACGACATCCGCCGCGATGGCGTGGTGCGAAGCGAAGCGGACGACACCCTGCCCGACGT
CGCCGACGACGCGAAGCCGCAGGACGAATGGCTGTGCGACCTTGAAGATGCCGAAGCGCTGCAACGCTGTCTCGAGGGGC
TGGAGAAGGACCATCGCCGTTCGATCCGCCTCGCCTTCTTCGAAGGGCTGTCGCATTCCGAACTGGCCCGCCGGATCGAT
GCCCCTCTGGGCACGGTGAAAAGCTGGATCCGGCGCGGCCTTGCAGGCCTGAAAGGATGCCTCGGTGGCTGA

Upstream 100 bases:

>100_bases
ATGAACGCAAGCATGGCAGCTTGCCTCACCCGCAGGAAAATCAACGCTTTGTGGATTGGCCATACATGGCTAGATGCCAT
CGAACGGGAGACAAGGGCCG

Downstream 100 bases:

>100_bases
TACGGCATCGCTTCACGAAGACGATTTCCTGCTCGCGGGCGAGCTGTCGCTCGGCGTACTCGAGGGCGAAGAGCTTTCCA
CCGCCCGGCGGCTGCAACTC

Product: sigma-70 region 2:sigma-70 region 4

Products: NA

Alternate protein names: Sigma-K factor [H]

Number of amino acids: Translated: 183; Mature: 182

Protein sequence:

>183_residues
MTQSRREALKLAMAGMAAGDRASLDTVYEMTSSKLFASILRVVRVRERAEDLLQDTYVKAWQRAGRFDPAKGSPITWLCT
IARNTALNDIRRDGVVRSEADDTLPDVADDAKPQDEWLCDLEDAEALQRCLEGLEKDHRRSIRLAFFEGLSHSELARRID
APLGTVKSWIRRGLAGLKGCLGG

Sequences:

>Translated_183_residues
MTQSRREALKLAMAGMAAGDRASLDTVYEMTSSKLFASILRVVRVRERAEDLLQDTYVKAWQRAGRFDPAKGSPITWLCT
IARNTALNDIRRDGVVRSEADDTLPDVADDAKPQDEWLCDLEDAEALQRCLEGLEKDHRRSIRLAFFEGLSHSELARRID
APLGTVKSWIRRGLAGLKGCLGG
>Mature_182_residues
TQSRREALKLAMAGMAAGDRASLDTVYEMTSSKLFASILRVVRVRERAEDLLQDTYVKAWQRAGRFDPAKGSPITWLCTI
ARNTALNDIRRDGVVRSEADDTLPDVADDAKPQDEWLCDLEDAEALQRCLEGLEKDHRRSIRLAFFEGLSHSELARRIDA
PLGTVKSWIRRGLAGLKGCLGG

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 20402; Mature: 20271

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQSRREALKLAMAGMAAGDRASLDTVYEMTSSKLFASILRVVRVRERAEDLLQDTYVKA
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WQRAGRFDPAKGSPITWLCTIARNTALNDIRRDGVVRSEADDTLPDVADDAKPQDEWLCD
HHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHCC
LEDAEALQRCLEGLEKDHRRSIRLAFFEGLSHSELARRIDAPLGTVKSWIRRGLAGLKGC
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LGG
CCC
>Mature Secondary Structure 
TQSRREALKLAMAGMAAGDRASLDTVYEMTSSKLFASILRVVRVRERAEDLLQDTYVKA
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WQRAGRFDPAKGSPITWLCTIARNTALNDIRRDGVVRSEADDTLPDVADDAKPQDEWLCD
HHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHCC
LEDAEALQRCLEGLEKDHRRSIRLAFFEGLSHSELARRIDAPLGTVKSWIRRGLAGLKGC
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LGG
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA