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The map label for this gene is manX [C]

Identifier: 85060404

GI number: 85060404

Start: 4164279

End: 4164728

Strand: Reverse

Name: manX [C]

Synonym: SG2426

Alternate gene names: 85060404

Gene position: 4164728-4164279 (Counterclockwise)

Preceding gene: 85060405

Following gene: 85060403

Centisome position: 99.85

GC content: 42.22

Gene sequence:

>450_bases
ATGAATAATATAAAACTGGTACGGGTCGATTTTCTCCTCATTCATGGGAAAGTCATTACAAAATGGCGAAATATTATTTC
CGCAACCGAAATCGTTATTGTGGATGACAAATTATCCAAAGACTCTTTTTTGGCCGAAATTTATGTGATGGCGCCACCAC
TAGGCGTCGATGTCCATGTCATGGCTGAAGAGGATTTTATTAAAGCCTCTCAGGAGGGGTACTTCGACACGAATAAAATC
AACATACTGGTTTTATTCAAATCCATCGGTAACATCAAGCATCTGATGGCAAAGGATATCGTATTTAATGCAGTTCAGGT
TGGGGAACTCGGTGGTGGTGTAAACCGGCGCAGCGTAGTGAACGACATATCTATTGATGATCAGGATTTACAGGATCTCA
AATTTATTCAGTCGCAGGGAACCGCCGTTTACTTCCAGGTCACCCCGTAG

Upstream 100 bases:

>100_bases
TACCGTAATGAATTAAATATTGAGGAACTGGTAAACCGAATTATTGAAGCAGGCGGTGAAAGTATCAAAAACGTGACATC
AATAGCTAACGGGAATACCT

Downstream 100 bases:

>100_bases
GAAGTCAAATTAACGTTGGATAAGGTCATTACGCGTCTGGAGGGTTGACTATGTGGGAAGTCGCGATTTTTACAGGGCTT
TGGTATTGGATATGTAAAAC

Product: putative PTS system IIB component

Products: protein histidine; sugar phosphate

Alternate protein names: PTS System IIB Component; PTS System Transporter Subunit IIB; Phosphotransferase System; Phosphotransferase System IIB; Protein-N(Pi)-Phosphohistidine-Sugarphosphotran Sferase; PTS System Protein

Number of amino acids: Translated: 149; Mature: 149

Protein sequence:

>149_residues
MNNIKLVRVDFLLIHGKVITKWRNIISATEIVIVDDKLSKDSFLAEIYVMAPPLGVDVHVMAEEDFIKASQEGYFDTNKI
NILVLFKSIGNIKHLMAKDIVFNAVQVGELGGGVNRRSVVNDISIDDQDLQDLKFIQSQGTAVYFQVTP

Sequences:

>Translated_149_residues
MNNIKLVRVDFLLIHGKVITKWRNIISATEIVIVDDKLSKDSFLAEIYVMAPPLGVDVHVMAEEDFIKASQEGYFDTNKI
NILVLFKSIGNIKHLMAKDIVFNAVQVGELGGGVNRRSVVNDISIDDQDLQDLKFIQSQGTAVYFQVTP
>Mature_149_residues
MNNIKLVRVDFLLIHGKVITKWRNIISATEIVIVDDKLSKDSFLAEIYVMAPPLGVDVHVMAEEDFIKASQEGYFDTNKI
NILVLFKSIGNIKHLMAKDIVFNAVQVGELGGGVNRRSVVNDISIDDQDLQDLKFIQSQGTAVYFQVTP

Specific function: This Is A Component Of The Phosphoenolpyruvate-Dependent Sugar Phosphotransferase System (Pts), A Major Carbohydrate Active -Transport System. The IIcd Domains Contain The Sugar Binding Site And The Transmembrane Channel; The IIa Domain Contains The Prim

COG id: COG3444

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IIB

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788120, Length=137, Percent_Identity=24.8175182481752, Blast_Score=64, Evalue=5e-12,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.1.69

Molecular weight: Translated: 16679; Mature: 16679

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS51101 PTS_EIIB_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNIKLVRVDFLLIHGKVITKWRNIISATEIVIVDDKLSKDSFLAEIYVMAPPLGVDVHV
CCCEEEEEEEEEEEECHHHHHHHHHHCEEEEEEECCCCCCCCEEEEEEEECCCCCCEEEE
MAEEDFIKASQEGYFDTNKINILVLFKSIGNIKHLMAKDIVFNAVQVGELGGGVNRRSVV
EECCCHHCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHEEECCCCCCCCCCCEE
NDISIDDQDLQDLKFIQSQGTAVYFQVTP
ECCCCCCCCHHHHHHHHCCCCEEEEEECC
>Mature Secondary Structure
MNNIKLVRVDFLLIHGKVITKWRNIISATEIVIVDDKLSKDSFLAEIYVMAPPLGVDVHV
CCCEEEEEEEEEEEECHHHHHHHHHHCEEEEEEECCCCCCCCEEEEEEEECCCCCCEEEE
MAEEDFIKASQEGYFDTNKINILVLFKSIGNIKHLMAKDIVFNAVQVGELGGGVNRRSVV
EECCCHHCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHEEECCCCCCCCCCCEE
NDISIDDQDLQDLKFIQSQGTAVYFQVTP
ECCCCCCCCHHHHHHHHCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA