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The map label for this gene is ilvE [H]

Identifier: 85060373

GI number: 85060373

Start: 4104606

End: 4105535

Strand: Reverse

Name: ilvE [H]

Synonym: SG2395

Alternate gene names: 85060373

Gene position: 4105535-4104606 (Counterclockwise)

Preceding gene: 85060374

Following gene: 85060372

Centisome position: 98.43

GC content: 56.34

Gene sequence:

>930_bases
ATGACAACGAAGAAAGCTGATTTCATTTGGTTTAACGGTGAAATGGTTCCGTGGGCAGAAGCCAAAGTCCATGTAATGTC
GCATGCGCTGCATTACGGGTCATCAGTCTTTGAAGGTGTACGTTGCTACGATTCGCATAAAGGCCCGGTGGTATTTCGTC
ATCGTGAACACATGCAGCGCCTACACAACTCGGCCAAAATCTATCGCATGCCGGTCACGCAAAGTGTCGATGAGCTTATG
GCGGCCACGCGCGCCGTTATACTGAAAAATAACCTGGTCAGCGCCTACATCCGACCGCTGGTGTTTGTCGGAGATGTCGG
CATGGGCGTTAACCCGCCGGCGGGCTATACCACCGACGTCATTATCGCCGCGTTCCCCTGGGGCGCTTATCTGGGCGAAG
AAGCCCTGGATCAGGGGATCGACGCGATGGTGTCTTCGTGGAACCGCGCGGCGGCCAATACTATCCCTACCGCGGTCAAA
GCCGGCGGCAATTACCTGTCTTCAATGCTGGTGGGCAGCGAAGCCCGCCGCCATGGTTATCAAGAGGGTATCGCGCTGGA
CGTACATGGTTATGTCTCCGAAGGTGCCGGCGAGAATTTGTTTGAAGTGAAGGACGGTATTTTGTTTACGCCGCCGTTCA
CCTCATCCGCGCTGCCCGGCATTACGCGCGACGCCATTATCAAGCTGGCCATCGCGGCCGGGCTGGAAGTGCGGGAACAG
GTCTTGTCCCGTGAGTCCCTCTATTTGGCCGACGAAGTCTTTATGTCCGGTACCGCCGCCGAGATTACCCCGGTGCGCAG
CGTTGACGGCATCAAAGTCGGCATCGGTAAATGCGGCCCGGTGACCAAGCAATTGCAGCAGGCGTTCTTCGGCTTGTTTA
CCGGCGAGACTGAAGATAAATGGGGCTGGCTGGATCCGGTCAATCAATAA

Upstream 100 bases:

>100_bases
TCTGCACAATTAAGCAAACTAATGGATGTTTCCTGTGTCGAGATCCAGCAATTAACAACACAAAAAATACGTGCCTAAGG
CCGGGTAAGGAAAGAAAAAA

Downstream 100 bases:

>100_bases
CGATAACGCTTTCGTGCCGCGGCGCTGCGGCTGCCCTGGCCATCTGAATAACTGGAGTTATGAGCATGCCTAAGTACCGT
TCCGCTACCACCACGCACGG

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT; Transaminase B [H]

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MTTKKADFIWFNGEMVPWAEAKVHVMSHALHYGSSVFEGVRCYDSHKGPVVFRHREHMQRLHNSAKIYRMPVTQSVDELM
AATRAVILKNNLVSAYIRPLVFVGDVGMGVNPPAGYTTDVIIAAFPWGAYLGEEALDQGIDAMVSSWNRAAANTIPTAVK
AGGNYLSSMLVGSEARRHGYQEGIALDVHGYVSEGAGENLFEVKDGILFTPPFTSSALPGITRDAIIKLAIAAGLEVREQ
VLSRESLYLADEVFMSGTAAEITPVRSVDGIKVGIGKCGPVTKQLQQAFFGLFTGETEDKWGWLDPVNQ

Sequences:

>Translated_309_residues
MTTKKADFIWFNGEMVPWAEAKVHVMSHALHYGSSVFEGVRCYDSHKGPVVFRHREHMQRLHNSAKIYRMPVTQSVDELM
AATRAVILKNNLVSAYIRPLVFVGDVGMGVNPPAGYTTDVIIAAFPWGAYLGEEALDQGIDAMVSSWNRAAANTIPTAVK
AGGNYLSSMLVGSEARRHGYQEGIALDVHGYVSEGAGENLFEVKDGILFTPPFTSSALPGITRDAIIKLAIAAGLEVREQ
VLSRESLYLADEVFMSGTAAEITPVRSVDGIKVGIGKCGPVTKQLQQAFFGLFTGETEDKWGWLDPVNQ
>Mature_308_residues
TTKKADFIWFNGEMVPWAEAKVHVMSHALHYGSSVFEGVRCYDSHKGPVVFRHREHMQRLHNSAKIYRMPVTQSVDELMA
ATRAVILKNNLVSAYIRPLVFVGDVGMGVNPPAGYTTDVIIAAFPWGAYLGEEALDQGIDAMVSSWNRAAANTIPTAVKA
GGNYLSSMLVGSEARRHGYQEGIALDVHGYVSEGAGENLFEVKDGILFTPPFTSSALPGITRDAIIKLAIAAGLEVREQV
LSRESLYLADEVFMSGTAAEITPVRSVDGIKVGIGKCGPVTKQLQQAFFGLFTGETEDKWGWLDPVNQ

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI50658084, Length=283, Percent_Identity=27.208480565371, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI38176287, Length=287, Percent_Identity=27.8745644599303, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI296010906, Length=287, Percent_Identity=27.8745644599303, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI296010904, Length=287, Percent_Identity=27.8745644599303, Blast_Score=85, Evalue=7e-17,
Organism=Escherichia coli, GI48994963, Length=309, Percent_Identity=88.673139158576, Blast_Score=581, Evalue=1e-167,
Organism=Caenorhabditis elegans, GI17565728, Length=287, Percent_Identity=29.2682926829268, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17568601, Length=282, Percent_Identity=28.0141843971631, Blast_Score=79, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6322608, Length=324, Percent_Identity=25.9259259259259, Blast_Score=85, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6322002, Length=313, Percent_Identity=25.5591054313099, Blast_Score=80, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24641779, Length=286, Percent_Identity=25.8741258741259, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 33650; Mature: 33519

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTKKADFIWFNGEMVPWAEAKVHVMSHALHYGSSVFEGVRCYDSHKGPVVFRHREHMQR
CCCCCCCEEEECCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHH
LHNSAKIYRMPVTQSVDELMAATRAVILKNNLVSAYIRPLVFVGDVGMGVNPPAGYTTDV
HHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCE
IIAAFPWGAYLGEEALDQGIDAMVSSWNRAAANTIPTAVKAGGNYLSSMLVGSEARRHGY
EEEECCCHHHHCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHCCH
QEGIALDVHGYVSEGAGENLFEVKDGILFTPPFTSSALPGITRDAIIKLAIAAGLEVREQ
HCCEEEEEECHHCCCCCCCHHHHCCCEEECCCCCCCCCCCCCHHHHHHHHHHHCHHHHHH
VLSRESLYLADEVFMSGTAAEITPVRSVDGIKVGIGKCGPVTKQLQQAFFGLFTGETEDK
HHHHHHHHHHHHHHHCCCCHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCCC
WGWLDPVNQ
CCCCCCCCC
>Mature Secondary Structure 
TTKKADFIWFNGEMVPWAEAKVHVMSHALHYGSSVFEGVRCYDSHKGPVVFRHREHMQR
CCCCCCEEEECCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHH
LHNSAKIYRMPVTQSVDELMAATRAVILKNNLVSAYIRPLVFVGDVGMGVNPPAGYTTDV
HHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCE
IIAAFPWGAYLGEEALDQGIDAMVSSWNRAAANTIPTAVKAGGNYLSSMLVGSEARRHGY
EEEECCCHHHHCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHCCH
QEGIALDVHGYVSEGAGENLFEVKDGILFTPPFTSSALPGITRDAIIKLAIAAGLEVREQ
HCCEEEEEECHHCCCCCCCHHHHCCCEEECCCCCCCCCCCCCHHHHHHHHHHHCHHHHHH
VLSRESLYLADEVFMSGTAAEITPVRSVDGIKVGIGKCGPVTKQLQQAFFGLFTGETEDK
HHHHHHHHHHHHHHHCCCCHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCCC
WGWLDPVNQ
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]