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The map label for this gene is wecC

Identifier: 85060360

GI number: 85060360

Start: 4086397

End: 4087659

Strand: Reverse

Name: wecC

Synonym: SG2382

Alternate gene names: 85060360

Gene position: 4087659-4086397 (Counterclockwise)

Preceding gene: 85060361

Following gene: 85060359

Centisome position: 98.0

GC content: 60.81

Gene sequence:

>1263_bases
ATGAGTTTTGACACAATTTCCGTAATTGGACTGGGGTACATCGGTCTGCCCACCGCCGCGGCGTTTGCCTCGCGCCGCAA
GCGGGTAATAGGGGTAGACACCAATCAGCACGCGGTGGATACCATTAACCGCGGCGCGATTCATATCATTGAACCCGATT
TGGACCGGGTCGTAACCGAAGCCGTGCACGAGGGGCTGCTGCGGGCGGTAAGCCAGCCGCAGCCGGCGGACGCGTTCCTT
ATTGCCGTACCAACCCCTTTTAAAGGCGCTCATCAACCCGATCTCAGCTATGTGCAGGCGGCGGCGTTAGCGTTAGCGCC
GGTGTTAACGCCGGGCTGTCTGGTGATCCTCGAATCCACCTCTCCGGTGGGGACAACCGAACAGATGGCCGACTGGCTGG
CCGGTGCACGTCCCGATCTGACCTTCCCACACCAGGTGGGCGAAGCGGCGGATATCAATATCGCCTATTGCCCGGAGCGC
GTGCTGCCCGGTAAGGTAATGGTGGAACTGATTAAAAATGACCGGGTGATCGGCGGTATGACGCCGCGCTGTTCGGCACG
CGCCGCCGAGCTGTATCGCATTTTCCTGGAAGGGGAGTGCGTCGTAACACATGTCCGTACCGCCGAAATGTGTAAATTGA
CCGAAAACAGCTTCCGCGATGTCAATATTGCCTTCGCTAATGAACTGTCGGTGCTCTGTGCGGAACAGCAGATTAATGTC
TGGGAACTGATAGCGCTGGCCAATCGCCATCCGCGGGTCAATATTCTGCAGCCGGGGCCAGGCGTCGGCGGCCATTGTAT
CGCGGTGGATCCCTGGTTCATTGTGGCGCAGAACCCGGCGCAGGCGCGGCTTATCCGCACCGCCCGCCAGGTCAACGATG
ATAAGCCCGACTGGGTGGTGGAAAAAGTAAAGGCGGCGGTGGTGGATTGCCTCAACGACAGCGGGCGCGGCAGTAGCGAT
GTGACCATCGCCTGCTTCGGGCTGGCGTTCAAGCCCGATATTGACGATTTGCGCGAAAGCCCCGCGCTGCACGTGACGCA
GCTGATTGCCCGTTGGCATCGCGGCCAGACGCTGGCGGTGGAGCCGAATGTCCATGCACTGCCGGCATCGCTGGACGGGC
TGGTCACGCTGGTTGACCGCGATCAGGCGCTGGCGACCGCCGATGTGCTGGTGATGCTGGTGGATCATCAACAGTTTCGC
GCCATGGCGCCTGGTGCAATTCACCAGCGCTGGGTGGTGGATACCCGGGGAGTCTGGCGATGA

Upstream 100 bases:

>100_bases
CAGGCGTATCAACGGATGAGTCGGGCACATAACCCCTACGGCGACGGCCACGCCTGTGAGCGGATTATCACGGTATTAAA
AAAAGATAGCAGGTGAATCC

Downstream 100 bases:

>100_bases
GATGCCTGCTGATTACCGGCGGCGCGGGGTTTATCGGCTCGGCGCTGGTGCGCTATATCCTTGGCGCTACCGACTATCGG
GTGCTGGTCGTCGACAAGTT

Product: UDP-N-acetyl-D-mannosamine dehydrogenase

Products: UDP-N-acetyl-D-mannosaminuronic-acid; NADH; Proton [C]

Alternate protein names: UDP-ManNAc 6-dehydrogenase [H]

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MSFDTISVIGLGYIGLPTAAAFASRRKRVIGVDTNQHAVDTINRGAIHIIEPDLDRVVTEAVHEGLLRAVSQPQPADAFL
IAVPTPFKGAHQPDLSYVQAAALALAPVLTPGCLVILESTSPVGTTEQMADWLAGARPDLTFPHQVGEAADINIAYCPER
VLPGKVMVELIKNDRVIGGMTPRCSARAAELYRIFLEGECVVTHVRTAEMCKLTENSFRDVNIAFANELSVLCAEQQINV
WELIALANRHPRVNILQPGPGVGGHCIAVDPWFIVAQNPAQARLIRTARQVNDDKPDWVVEKVKAAVVDCLNDSGRGSSD
VTIACFGLAFKPDIDDLRESPALHVTQLIARWHRGQTLAVEPNVHALPASLDGLVTLVDRDQALATADVLVMLVDHQQFR
AMAPGAIHQRWVVDTRGVWR

Sequences:

>Translated_420_residues
MSFDTISVIGLGYIGLPTAAAFASRRKRVIGVDTNQHAVDTINRGAIHIIEPDLDRVVTEAVHEGLLRAVSQPQPADAFL
IAVPTPFKGAHQPDLSYVQAAALALAPVLTPGCLVILESTSPVGTTEQMADWLAGARPDLTFPHQVGEAADINIAYCPER
VLPGKVMVELIKNDRVIGGMTPRCSARAAELYRIFLEGECVVTHVRTAEMCKLTENSFRDVNIAFANELSVLCAEQQINV
WELIALANRHPRVNILQPGPGVGGHCIAVDPWFIVAQNPAQARLIRTARQVNDDKPDWVVEKVKAAVVDCLNDSGRGSSD
VTIACFGLAFKPDIDDLRESPALHVTQLIARWHRGQTLAVEPNVHALPASLDGLVTLVDRDQALATADVLVMLVDHQQFR
AMAPGAIHQRWVVDTRGVWR
>Mature_419_residues
SFDTISVIGLGYIGLPTAAAFASRRKRVIGVDTNQHAVDTINRGAIHIIEPDLDRVVTEAVHEGLLRAVSQPQPADAFLI
AVPTPFKGAHQPDLSYVQAAALALAPVLTPGCLVILESTSPVGTTEQMADWLAGARPDLTFPHQVGEAADINIAYCPERV
LPGKVMVELIKNDRVIGGMTPRCSARAAELYRIFLEGECVVTHVRTAEMCKLTENSFRDVNIAFANELSVLCAEQQINVW
ELIALANRHPRVNILQPGPGVGGHCIAVDPWFIVAQNPAQARLIRTARQVNDDKPDWVVEKVKAAVVDCLNDSGRGSSDV
TIACFGLAFKPDIDDLRESPALHVTQLIARWHRGQTLAVEPNVHALPASLDGLVTLVDRDQALATADVLVMLVDHQQFRA
MAPGAIHQRWVVDTRGVWR

Specific function: Catalyzes the four-electron oxidation of UDP-N-acetyl-D- mannosamine (UDP-ManNAc), reducing NAD(+) and releasing UDP-N- acetylmannosaminuronic acid (UDP-ManNAcA) [H]

COG id: COG0677

COG function: function code M; UDP-N-acetyl-D-mannosaminuronate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UDP-glucose/GDP-mannose dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4507813, Length=368, Percent_Identity=26.9021739130435, Blast_Score=92, Evalue=9e-19,
Organism=Escherichia coli, GI48994968, Length=420, Percent_Identity=75.9523809523809, Blast_Score=670, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17560350, Length=368, Percent_Identity=27.1739130434783, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI17136908, Length=365, Percent_Identity=26.3013698630137, Blast_Score=95, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR016040
- InterPro:   IPR017476
- InterPro:   IPR014027
- InterPro:   IPR014026
- InterPro:   IPR014028
- InterPro:   IPR001732 [H]

Pfam domain/function: PF00984 UDPG_MGDP_dh; PF03720 UDPG_MGDP_dh_C; PF03721 UDPG_MGDP_dh_N [H]

EC number: 1.1.1.- [C]

Molecular weight: Translated: 45642; Mature: 45511

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFDTISVIGLGYIGLPTAAAFASRRKRVIGVDTNQHAVDTINRGAIHIIEPDLDRVVTE
CCCCEEEEEEECCCCHHHHHHHHHCCCEEEEECCCCHHHHHHCCCEEEEECCCHHHHHHH
AVHEGLLRAVSQPQPADAFLIAVPTPFKGAHQPDLSYVQAAALALAPVLTPGCLVILEST
HHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECC
SPVGTTEQMADWLAGARPDLTFPHQVGEAADINIAYCPERVLPGKVMVELIKNDRVIGGM
CCCCCHHHHHHHHCCCCCCCCCCHHCCCCCCCEEEECCCHHCCHHHHHHHHHCCCEEECC
TPRCSARAAELYRIFLEGECVVTHVRTAEMCKLTENSFRDVNIAFANELSVLCAEQQINV
CCCCHHHHHHHEEEEECCCEEEEECCHHHHHHHHCCCCCEEEEEEECCCEEEEECCCCCH
WELIALANRHPRVNILQPGPGVGGHCIAVDPWFIVAQNPAQARLIRTARQVNDDKPDWVV
HHHHHHHCCCCEEEEECCCCCCCCEEEEECCEEEEECCCHHHHHHHHHHHCCCCCCHHHH
EKVKAAVVDCLNDSGRGSSDVTIACFGLAFKPDIDDLRESPALHVTQLIARWHRGQTLAV
HHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCCHHHHHCCCCHHHHHHHHHHHCCCEEEE
EPNVHALPASLDGLVTLVDRDQALATADVLVMLVDHQQFRAMAPGAIHQRWVVDTRGVWR
CCCCEECCCCCCCEEEEEECCCHHHHHHHHHHEECCHHHHHCCCCCHHCEEEECCCCCCC
>Mature Secondary Structure 
SFDTISVIGLGYIGLPTAAAFASRRKRVIGVDTNQHAVDTINRGAIHIIEPDLDRVVTE
CCCEEEEEEECCCCHHHHHHHHHCCCEEEEECCCCHHHHHHCCCEEEEECCCHHHHHHH
AVHEGLLRAVSQPQPADAFLIAVPTPFKGAHQPDLSYVQAAALALAPVLTPGCLVILEST
HHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECC
SPVGTTEQMADWLAGARPDLTFPHQVGEAADINIAYCPERVLPGKVMVELIKNDRVIGGM
CCCCCHHHHHHHHCCCCCCCCCCHHCCCCCCCEEEECCCHHCCHHHHHHHHHCCCEEECC
TPRCSARAAELYRIFLEGECVVTHVRTAEMCKLTENSFRDVNIAFANELSVLCAEQQINV
CCCCHHHHHHHEEEEECCCEEEEECCHHHHHHHHCCCCCEEEEEEECCCEEEEECCCCCH
WELIALANRHPRVNILQPGPGVGGHCIAVDPWFIVAQNPAQARLIRTARQVNDDKPDWVV
HHHHHHHCCCCEEEEECCCCCCCCEEEEECCEEEEECCCHHHHHHHHHHHCCCCCCHHHH
EKVKAAVVDCLNDSGRGSSDVTIACFGLAFKPDIDDLRESPALHVTQLIARWHRGQTLAV
HHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCCHHHHHCCCCHHHHHHHHHHHCCCEEEE
EPNVHALPASLDGLVTLVDRDQALATADVLVMLVDHQQFRAMAPGAIHQRWVVDTRGVWR
CCCCEECCCCCCCEEEEEECCCHHHHHHHHHHEECCHHHHHCCCCCHHCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-N-acetyl-D-mannosamine; NAD [C]

Specific reaction: UDP-N-acetyl-D-mannosamine + NAD = UDP-N-acetyl-D-mannosaminuronic-acid + NADH + Proton [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]