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The map label for this gene is rffG [H]

Identifier: 85060359

GI number: 85060359

Start: 4085327

End: 4086400

Strand: Reverse

Name: rffG [H]

Synonym: SG2381

Alternate gene names: 85060359

Gene position: 4086400-4085327 (Counterclockwise)

Preceding gene: 85060360

Following gene: 85060358

Centisome position: 97.97

GC content: 61.08

Gene sequence:

>1074_bases
ATGAGATGCCTGCTGATTACCGGCGGCGCGGGGTTTATCGGCTCGGCGCTGGTGCGCTATATCCTTGGCGCTACCGACTA
TCGGGTGCTGGTCGTCGACAAGTTGACCTATGCCGGCAATCTGGACTCGCTCGCGCCGGTGGCAGGGCATCCCCATTATC
GCTTCGCCCGCGCCGATATCGGCGATGGCCCGGCGATGGCGCGGCTGCTCGCGGAGTTTCAGCCTTACGCCATCATGCAT
CTGGCGGCGGAAAGCCATGTCGATCGCTCCATTGACGGTCCGGCGGCGTTTATCGATACCAATATCACCGGGACCTCGAT
CCTTCTGGAAGCGGCGCGCGAATATTGGCAGGCTTTACCCCCCGCTGCCCGCGCCGCTTTTCGCTTTCATCATGTCTCCA
CCGATGAAGTATACGGCGATTTGCCCGATGACGGCAGCCGGTTTAACGAAAGCTCGCCCTATGCGCCCAGCAGCCCGTAC
TCCGCCTCGAAAGCCGCCAGCGATCATTTGGCGCGCGCCTGGATGCGCACTTACGGTCTGCCGGTGCTGGTAACCCATTG
CTCCAATAATTATGGACCCTATCATTTTCCGGAAAAGCTGATTCCGCTGATGATTATCAACGCGCTGGCGGGCAAGCCGT
TACCGGTTTACGGCGACGGCGGCCAGATTCGTGACTGGCTGTATGTGGAAGATCATGCCCGCGCGCTCTATCAGGTGGTG
ACCCGCGGCCGTCCCGGTGAAACCTATACCATCGGCGGCCATAACGAGCGGCGCAATATTGAGGTGGTCGAGGCGCTGTG
CGCGCTATTGGAGCAGGCTGGTGCGCCTCATCCTGCCGGCGTCACGGCGTTCAGGCAACTCATTACGCTTGTAGAAGATC
GGCCGGGGCATGACCGGCGCTATGCGATTGACGCCGGCAAAATTGCCCGGGAATTAGGCTGGCGGCCGCAGGAAACGTTT
GACAGCGGCCTGGCGAAAACCGTGCAGTGGTATTTGACCCATCGTGAGTGGTGGCAGCGCGTTCTGGACGGCAGCTATCG
CGGCGAACGTCTCGGTCTCGGGCCAGGCTTGTAA

Upstream 100 bases:

>100_bases
CCGATGTGCTGGTGATGCTGGTGGATCATCAACAGTTTCGCGCCATGGCGCCTGGTGCAATTCACCAGCGCTGGGTGGTG
GATACCCGGGGAGTCTGGCG

Downstream 100 bases:

>100_bases
GGCGAGGAGTGCAGATGAAAGGTATTGTCTTGGCGGGCGGCAGCGGCAGTCGCCTCTATCCGATTACCCGCGGTATTTCC
AAACAGCTGTTGCCCGTGTA

Product: dTDP-D-glucose-4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MRCLLITGGAGFIGSALVRYILGATDYRVLVVDKLTYAGNLDSLAPVAGHPHYRFARADIGDGPAMARLLAEFQPYAIMH
LAAESHVDRSIDGPAAFIDTNITGTSILLEAAREYWQALPPAARAAFRFHHVSTDEVYGDLPDDGSRFNESSPYAPSSPY
SASKAASDHLARAWMRTYGLPVLVTHCSNNYGPYHFPEKLIPLMIINALAGKPLPVYGDGGQIRDWLYVEDHARALYQVV
TRGRPGETYTIGGHNERRNIEVVEALCALLEQAGAPHPAGVTAFRQLITLVEDRPGHDRRYAIDAGKIARELGWRPQETF
DSGLAKTVQWYLTHREWWQRVLDGSYRGERLGLGPGL

Sequences:

>Translated_357_residues
MRCLLITGGAGFIGSALVRYILGATDYRVLVVDKLTYAGNLDSLAPVAGHPHYRFARADIGDGPAMARLLAEFQPYAIMH
LAAESHVDRSIDGPAAFIDTNITGTSILLEAAREYWQALPPAARAAFRFHHVSTDEVYGDLPDDGSRFNESSPYAPSSPY
SASKAASDHLARAWMRTYGLPVLVTHCSNNYGPYHFPEKLIPLMIINALAGKPLPVYGDGGQIRDWLYVEDHARALYQVV
TRGRPGETYTIGGHNERRNIEVVEALCALLEQAGAPHPAGVTAFRQLITLVEDRPGHDRRYAIDAGKIARELGWRPQETF
DSGLAKTVQWYLTHREWWQRVLDGSYRGERLGLGPGL
>Mature_357_residues
MRCLLITGGAGFIGSALVRYILGATDYRVLVVDKLTYAGNLDSLAPVAGHPHYRFARADIGDGPAMARLLAEFQPYAIMH
LAAESHVDRSIDGPAAFIDTNITGTSILLEAAREYWQALPPAARAAFRFHHVSTDEVYGDLPDDGSRFNESSPYAPSSPY
SASKAASDHLARAWMRTYGLPVLVTHCSNNYGPYHFPEKLIPLMIINALAGKPLPVYGDGGQIRDWLYVEDHARALYQVV
TRGRPGETYTIGGHNERRNIEVVEALCALLEQAGAPHPAGVTAFRQLITLVEDRPGHDRRYAIDAGKIARELGWRPQETF
DSGLAKTVQWYLTHREWWQRVLDGSYRGERLGLGPGL

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=339, Percent_Identity=34.5132743362832, Blast_Score=214, Evalue=9e-56,
Organism=Homo sapiens, GI42516563, Length=341, Percent_Identity=26.3929618768328, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI4504031, Length=345, Percent_Identity=22.0289855072464, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI48994969, Length=353, Percent_Identity=71.1048158640227, Blast_Score=527, Evalue=1e-151,
Organism=Escherichia coli, GI1788353, Length=353, Percent_Identity=64.3059490084986, Blast_Score=471, Evalue=1e-134,
Organism=Escherichia coli, GI1788366, Length=355, Percent_Identity=24.7887323943662, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1786974, Length=359, Percent_Identity=26.7409470752089, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17568069, Length=333, Percent_Identity=35.7357357357357, Blast_Score=204, Evalue=7e-53,
Organism=Caenorhabditis elegans, GI115532424, Length=333, Percent_Identity=30.6306306306306, Blast_Score=156, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17539532, Length=346, Percent_Identity=25.7225433526012, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17507723, Length=257, Percent_Identity=24.5136186770428, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17539422, Length=257, Percent_Identity=24.5136186770428, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI133901788, Length=257, Percent_Identity=24.5136186770428, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI133901786, Length=259, Percent_Identity=24.3243243243243, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17539424, Length=257, Percent_Identity=24.5136186770428, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI133901790, Length=257, Percent_Identity=24.5136186770428, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6319493, Length=364, Percent_Identity=25.2747252747253, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21356223, Length=341, Percent_Identity=27.8592375366569, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI19923002, Length=362, Percent_Identity=26.7955801104972, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24158427, Length=345, Percent_Identity=24.6376811594203, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 39407; Mature: 39407

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRCLLITGGAGFIGSALVRYILGATDYRVLVVDKLTYAGNLDSLAPVAGHPHYRFARADI
CEEEEEECCCHHHHHHHHHHHHCCCCEEEEEEEEEHHCCCCCCCCCCCCCCCCEEEECCC
GDGPAMARLLAEFQPYAIMHLAAESHVDRSIDGPAAFIDTNITGTSILLEAAREYWQALP
CCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCC
PAARAAFRFHHVSTDEVYGDLPDDGSRFNESSPYAPSSPYSASKAASDHLARAWMRTYGL
HHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
PVLVTHCSNNYGPYHFPEKLIPLMIINALAGKPLPVYGDGGQIRDWLYVEDHARALYQVV
EEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCEEEEEEEHHHHHHHHHHH
TRGRPGETYTIGGHNERRNIEVVEALCALLEQAGAPHPAGVTAFRQLITLVEDRPGHDRR
HCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCE
YAIDAGKIARELGWRPQETFDSGLAKTVQWYLTHREWWQRVLDGSYRGERLGLGPGL
EEECHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRCLLITGGAGFIGSALVRYILGATDYRVLVVDKLTYAGNLDSLAPVAGHPHYRFARADI
CEEEEEECCCHHHHHHHHHHHHCCCCEEEEEEEEEHHCCCCCCCCCCCCCCCCEEEECCC
GDGPAMARLLAEFQPYAIMHLAAESHVDRSIDGPAAFIDTNITGTSILLEAAREYWQALP
CCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCC
PAARAAFRFHHVSTDEVYGDLPDDGSRFNESSPYAPSSPYSASKAASDHLARAWMRTYGL
HHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
PVLVTHCSNNYGPYHFPEKLIPLMIINALAGKPLPVYGDGGQIRDWLYVEDHARALYQVV
EEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCEEEEEEEHHHHHHHHHHH
TRGRPGETYTIGGHNERRNIEVVEALCALLEQAGAPHPAGVTAFRQLITLVEDRPGHDRR
HCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCE
YAIDAGKIARELGWRPQETFDSGLAKTVQWYLTHREWWQRVLDGSYRGERLGLGPGL
EEECHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 7559340 [H]