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The map label for this gene is pldA [H]

Identifier: 85060316

GI number: 85060316

Start: 4014996

End: 4015865

Strand: Reverse

Name: pldA [H]

Synonym: SG2338

Alternate gene names: 85060316

Gene position: 4015865-4014996 (Counterclockwise)

Preceding gene: 85060319

Following gene: 85060315

Centisome position: 96.28

GC content: 52.99

Gene sequence:

>870_bases
ATGCGCAAACTTTGGGGGGCAACCGCTTTCTTGCTGGGATCGACTTCTTTCGTTTACGCTCAAGAAGCCACGGTTGAGAA
GATCCATGATACACCGGCGGTGAAGGGAAGCATCATTGCCAATCTGTTACAGGAGCACGACAATCCCTTCACGCTTTACC
CGTATGAGAGCAACTACCTGCTCTACACCTATACCAGCAACATCAATAAACAGGCGATAAGCAGCTACGATTGGGCCAAT
AACGCCAAAAAAGACGAAGTGGCTTTCCAATTAAGCCTGGGATTTCCGTTGTGGCGCGGTATCGCGGGCGATAATTCGTT
GCTGGGCGCGTCCTATACCCAGCGCTCTTGGTGGCAGTTATCCAACAGTGAAGAATCGTCGCCCTTTCGCGAAACCAACT
ATGAACCTCAGCTGTTCATTGGCTGGGCAACGGATTATGATTTCGCAGGCTGGACCTTGCGCGATGTGGAGCTGGGTCTG
AACCATCAGTCCAACGGCCGCTCGGATCCCACTTCGCGCAGCTGGAACCGCGCCTATACGCGGCTCATGGCGCAAAACGG
GAACTGGCAGGTAGAAGTGAAGCCGTGGCTGCGGCTGGACGACAGCAGCGATAATCCCGATATTATCAAGTATATGGGCC
ACTACCGGCTGAAGATAGGTTATGCCTGGGGAGAAAGTATCTTCAGCGCGGAAGGACGCTACAACTGGAACAGCGGCTAC
GGTGGAGCGCAGGTGGGCTGGAGCTATCCCGTCAGCCGGCACGTCCGTTTTTATACCCAGGTGTTTAGTGGTTATGGCGA
GTCGCTAATCGATTATAATCATCGCCAGACGTGTATCGGGATGGGCTTTATGCTCAATGATATGATGTAA

Upstream 100 bases:

>100_bases
ACTTTAGGCAGTTTTCCCTAAAACAAAGTTCTAAGAGCGTGTAAAATGATTAGCTTTGAATGATTTTGTTGGTGGGTTAC
GCGCTTTCGGAGAACATTTT

Downstream 100 bases:

>100_bases
TTCACAGGGACTTAACCACGGACGCCGCTGCGGCGTTCGTTTGAAGACAAAGTTGGAGCAGATGTGTCTACGGCGGAAAT
AATCCAGCGGGAAGTGTTGG

Product: phospholipase A

Products: NA

Alternate protein names: Detergent-resistant phospholipase A; DR-phospholipase A; Outer membrane phospholipase A; OM PLA; OMPLA; Phosphatidylcholine 1-acylhydrolase [H]

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MRKLWGATAFLLGSTSFVYAQEATVEKIHDTPAVKGSIIANLLQEHDNPFTLYPYESNYLLYTYTSNINKQAISSYDWAN
NAKKDEVAFQLSLGFPLWRGIAGDNSLLGASYTQRSWWQLSNSEESSPFRETNYEPQLFIGWATDYDFAGWTLRDVELGL
NHQSNGRSDPTSRSWNRAYTRLMAQNGNWQVEVKPWLRLDDSSDNPDIIKYMGHYRLKIGYAWGESIFSAEGRYNWNSGY
GGAQVGWSYPVSRHVRFYTQVFSGYGESLIDYNHRQTCIGMGFMLNDMM

Sequences:

>Translated_289_residues
MRKLWGATAFLLGSTSFVYAQEATVEKIHDTPAVKGSIIANLLQEHDNPFTLYPYESNYLLYTYTSNINKQAISSYDWAN
NAKKDEVAFQLSLGFPLWRGIAGDNSLLGASYTQRSWWQLSNSEESSPFRETNYEPQLFIGWATDYDFAGWTLRDVELGL
NHQSNGRSDPTSRSWNRAYTRLMAQNGNWQVEVKPWLRLDDSSDNPDIIKYMGHYRLKIGYAWGESIFSAEGRYNWNSGY
GGAQVGWSYPVSRHVRFYTQVFSGYGESLIDYNHRQTCIGMGFMLNDMM
>Mature_289_residues
MRKLWGATAFLLGSTSFVYAQEATVEKIHDTPAVKGSIIANLLQEHDNPFTLYPYESNYLLYTYTSNINKQAISSYDWAN
NAKKDEVAFQLSLGFPLWRGIAGDNSLLGASYTQRSWWQLSNSEESSPFRETNYEPQLFIGWATDYDFAGWTLRDVELGL
NHQSNGRSDPTSRSWNRAYTRLMAQNGNWQVEVKPWLRLDDSSDNPDIIKYMGHYRLKIGYAWGESIFSAEGRYNWNSGY
GGAQVGWSYPVSRHVRFYTQVFSGYGESLIDYNHRQTCIGMGFMLNDMM

Specific function: Hydrolysis of phosphatidylcholine with phospholipase A2 (EC 3.1.1.4) and phospholipase A1 (EC 3.1.1.32) activities [H]

COG id: COG2829

COG function: function code M; Outer membrane phospholipase A

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein. Note=One of the very few enzymes located there (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phospholipase A1 family [H]

Homologues:

Organism=Escherichia coli, GI2367300, Length=291, Percent_Identity=72.8522336769759, Blast_Score=452, Evalue=1e-128,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003187 [H]

Pfam domain/function: PF02253 PLA1 [H]

EC number: =3.1.1.32; =3.1.1.4 [H]

Molecular weight: Translated: 33214; Mature: 33214

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLWGATAFLLGSTSFVYAQEATVEKIHDTPAVKGSIIANLLQEHDNPFTLYPYESNYL
CCCHHCCEEEEECCCEEEEECHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCEE
LYTYTSNINKQAISSYDWANNAKKDEVAFQLSLGFPLWRGIAGDNSLLGASYTQRSWWQL
EEEEECCCCHHHHHCCCCCCCCCCCCEEEEEEECCHHHHCCCCCCCEECCCCCCCHHEEE
SNSEESSPFRETNYEPQLFIGWATDYDFAGWTLRDVELGLNHQSNGRSDPTSRSWNRAYT
CCCCCCCCCHHCCCCCEEEEEEECCCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHH
RLMAQNGNWQVEVKPWLRLDDSSDNPDIIKYMGHYRLKIGYAWGESIFSAEGRYNWNSGY
HHHCCCCCEEEEEECEEEECCCCCCCHHHEECCCEEEEEEEEECCHHHCCCCCCCCCCCC
GGAQVGWSYPVSRHVRFYTQVFSGYGESLIDYNHRQTCIGMGFMLNDMM
CCCCCCCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCEEEEHHHHHHCCC
>Mature Secondary Structure
MRKLWGATAFLLGSTSFVYAQEATVEKIHDTPAVKGSIIANLLQEHDNPFTLYPYESNYL
CCCHHCCEEEEECCCEEEEECHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCEE
LYTYTSNINKQAISSYDWANNAKKDEVAFQLSLGFPLWRGIAGDNSLLGASYTQRSWWQL
EEEEECCCCHHHHHCCCCCCCCCCCCEEEEEEECCHHHHCCCCCCCEECCCCCCCHHEEE
SNSEESSPFRETNYEPQLFIGWATDYDFAGWTLRDVELGLNHQSNGRSDPTSRSWNRAYT
CCCCCCCCCHHCCCCCEEEEEEECCCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHH
RLMAQNGNWQVEVKPWLRLDDSSDNPDIIKYMGHYRLKIGYAWGESIFSAEGRYNWNSGY
HHHCCCCCEEEEEECEEEECCCCCCCHHHEECCCEEEEEEEEECCHHHCCCCCCCCCCCC
GGAQVGWSYPVSRHVRFYTQVFSGYGESLIDYNHRQTCIGMGFMLNDMM
CCCCCCCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCEEEEHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]