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The map label for this gene is rhtC [H]

Identifier: 85060314

GI number: 85060314

Start: 4012389

End: 4013024

Strand: Reverse

Name: rhtC [H]

Synonym: SG2336

Alternate gene names: 85060314

Gene position: 4013024-4012389 (Counterclockwise)

Preceding gene: 85060315

Following gene: 85060313

Centisome position: 96.21

GC content: 61.48

Gene sequence:

>636_bases
TTGGCAGCGGCGTCCCGGGATAGGTACCCCCTGACGCGTGGGGCATCGTGCCTCGGCGTTTTATTTCTGGAGCAGGAAAA
CTATGTTTATGCTGTTTGTTACCGTGGCGCTGGTGCACCTGATTGCCCTGATGTCGCCCGGTCCCGATTCCTTTTTCGTC
TCGCAGACGGCCGCCAGCCGCTCGCGTCGCCAGGCGATGTACGGCGTCCTGGGCATTATCCTTGGGGTAGCCATCTGGGC
GGCGGTCTGTATCTTTGCTGGATGGGCTGGCAATTGCTGCGCGCAGCGCGCCGACGGGCGCCCGCGGGTGACGCGCCGGC
CGTGGAGGTTGCCGCCAGCGGCGGGTGGGAACATCGCTTTTTGCGCGGTTTGTTAACCAATCTAGCCAACCCCAAGGCGG
TCATTTACTTTGGTATCGTCTTCTCGCTCTTCGTTGGCGATGATGTGGGCGCCGGCGCCCGCTTAGGAATATTTGCCCTG
ATTTTGGGCGAAACCCTGCTGTGGTTTTCCCTGGTGGCGGTGGTATTCGCCCTGCCGGCGATGCGCCGCGGCTATCAGCG
TCTTGCGCGCTGGATTGATGGCGTGGCCGGTGTGTTGTTTGCCGGCTTCGGCATTAATCTCATTTTGTCGCGCTGA

Upstream 100 bases:

>100_bases
CGGTCTGCCGTTTCTGGCCCTTATCCGTGAACACCTGTCTGATGAGGGCTCGCGGCCGACCCTGCGTTAAAGGCGGTAAG
GCCGATAGGTTCGCCAGGCT

Downstream 100 bases:

>100_bases
GGCGACGCCGGGCGATGTGCCGGGTGCGTAACGCGGAGTGGTTAACCCGTGAACGGCAATTTGCCGCGTTCTCTGCCGGC
GAGCTGCTGGATTTTTGGCG

Product: threonine efflux transport protein

Products: Proton [Cytoplasm]; L-threonine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MAAASRDRYPLTRGASCLGVLFLEQENYVYAVCYRGAGAPDCPDVARSRFLFRLADGRQPLASPGDVRRPGHYPWGSHLG
GGLYLCWMGWQLLRAARRRAPAGDAPAVEVAASGGWEHRFLRGLLTNLANPKAVIYFGIVFSLFVGDDVGAGARLGIFAL
ILGETLLWFSLVAVVFALPAMRRGYQRLARWIDGVAGVLFAGFGINLILSR

Sequences:

>Translated_211_residues
MAAASRDRYPLTRGASCLGVLFLEQENYVYAVCYRGAGAPDCPDVARSRFLFRLADGRQPLASPGDVRRPGHYPWGSHLG
GGLYLCWMGWQLLRAARRRAPAGDAPAVEVAASGGWEHRFLRGLLTNLANPKAVIYFGIVFSLFVGDDVGAGARLGIFAL
ILGETLLWFSLVAVVFALPAMRRGYQRLARWIDGVAGVLFAGFGINLILSR
>Mature_210_residues
AAASRDRYPLTRGASCLGVLFLEQENYVYAVCYRGAGAPDCPDVARSRFLFRLADGRQPLASPGDVRRPGHYPWGSHLGG
GLYLCWMGWQLLRAARRRAPAGDAPAVEVAASGGWEHRFLRGLLTNLANPKAVIYFGIVFSLFVGDDVGAGARLGIFALI
LGETLLWFSLVAVVFALPAMRRGYQRLARWIDGVAGVLFAGFGINLILSR

Specific function: Conducts the efflux of threonine [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994978, Length=134, Percent_Identity=70.1492537313433, Blast_Score=182, Evalue=1e-47,
Organism=Escherichia coli, GI1786522, Length=128, Percent_Identity=32.8125, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22943; Mature: 22812

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAASRDRYPLTRGASCLGVLFLEQENYVYAVCYRGAGAPDCPDVARSRFLFRLADGRQP
CCCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCC
LASPGDVRRPGHYPWGSHLGGGLYLCWMGWQLLRAARRRAPAGDAPAVEVAASGGWEHRF
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHH
LRGLLTNLANPKAVIYFGIVFSLFVGDDVGAGARLGIFALILGETLLWFSLVAVVFALPA
HHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MRRGYQRLARWIDGVAGVLFAGFGINLILSR
HHHHHHHHHHHHHHHHHHHHHHCCCEEEECC
>Mature Secondary Structure 
AAASRDRYPLTRGASCLGVLFLEQENYVYAVCYRGAGAPDCPDVARSRFLFRLADGRQP
CCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCC
LASPGDVRRPGHYPWGSHLGGGLYLCWMGWQLLRAARRRAPAGDAPAVEVAASGGWEHRF
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHH
LRGLLTNLANPKAVIYFGIVFSLFVGDDVGAGARLGIFALILGETLLWFSLVAVVFALPA
HHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MRRGYQRLARWIDGVAGVLFAGFGINLILSR
HHHHHHHHHHHHHHHHHHHHHHCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; L-threonine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + L-threonine [Cytoplasm] = Proton [Cytoplasm] + L-threonine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]