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The map label for this gene is gntT [H]

Identifier: 85060310

GI number: 85060310

Start: 4007119

End: 4008435

Strand: Reverse

Name: gntT [H]

Synonym: SG2332

Alternate gene names: 85060310

Gene position: 4008435-4007119 (Counterclockwise)

Preceding gene: 85060311

Following gene: 85060308

Centisome position: 96.1

GC content: 56.11

Gene sequence:

>1317_bases
ATGCCACTTTTTATTGTTGCCATTGGGGTAGCACTCCTGTTGCTCCTGATGATCCGTTTTAAACTAAACGGTTTCATCGC
GTTAATCCTGGTTGCACTGGCTGTAGGGATCCTGCAGGGCATGCCGGTCAACAAAGTCATCGAATCGATCAAAAACGGCG
TTGGCGGCACGCTGGGCAGTCTGGCGCTGATTATGGGTTTCGGCGCCATGCTGGGAAAATTGCTGGCGGATTGCGGCGGT
GCTCAGCGTATTGCCACCACGCTTATCGACAAATTTGGACAGCTTTATATTCAATGGGCGGTGGTATTAACGGGTTTCAC
CGTCGGTTTCGCGCTGTTTTATGAGATTGGTTTTGTGCTGATGCTGCCGCTGGTGTTCAGTATCGCCGCCAACGCGCGTA
TTTCGCTACTGTACGTGGGCGTACCAATGGCCGCCGCGCTGTCGGTAACGCACGGCTTCCTGCCGCCGCACCCTGGCCCT
ACCGCGATTGCCGCCATCTTCCACGCTGACATGGGCAGAACGCTGTTATACGGCACCATCCTCGCCATTCCGACGGTCAT
TCTGGCCGGACCGGTCTATTCGCGTTTCCTGTTCCATATTGACAAGCCGGTGCCGGAAGGTCTGTATAACCCGAAAAAGT
TTACCGAGCAGGAGATGCCGAGCTTTGCCGTCAGCGTCTGGACTTCGCTGGTACCGGTGGTATTAATGGCGCTGCGTGCC
GTTACCGAAATGGTATTACCGGCGGGCAATCCCATCCTGGCGTATACCGAGTTCTTCGGCGAACCGATTATGGCCACGTT
TATCGCCGTGCTGATCGCCATCTTCACGTTCGGTCTTAATCGCGGCCGCTCTATGGATGAGGTGATGAATACCATTACCG
ATTCCATAAAAATTATCGCCATGATGCTCTTGATTATCGGCGGCGGCGGGGCCTTTAAACAGGTCCTGGTAGACAGCGGC
GTGGATAAATATATCGCTTCGCTGATGGAAGGCAGCACCATTTCACCGTTGTTGCTCGCCTGGACCATCGCCGCTACGCT
GCGCATTGCGCTGGGTTCGGCGACGGTCGCCGCGATCACCGCCGGCGGTATCGCCGCACCGCTTATCGCCACCACCGGCG
TCAGCCCCGAGCTGATGGTTATCGCCGTCGGCTCCGGCAGCGTGATCTTTTCTCACGTCAATGACCCCGGCTTCTGGCTG
TTCAAGGAGTATTTCAACCTGACCATTGGCGAGACCATCCGCTCCTGGTCAGTGCTGGAAACCATCATCGCGGTCGGCGG
CCTGGTGGGCTGTCTGCTGCTGAATATGGTGATTTAA

Upstream 100 bases:

>100_bases
TACCGGTAACATCATATCATAAGATATGATTTGAAGACTATCATATATTGACCGGCGCGCGACTCACCGGTTCGGACAGA
TATCCATTGGGGGAATTATC

Downstream 100 bases:

>100_bases
GCTTTAGCGAATAGCACGTGGTAACACGTGCGGTCCGGCGTGGCAGTGTGCCCGCCGGGCCTTATTTTATCTTGTCGGCC
TGCCGGCTCCCTTACCCGAG

Product: high-affinity gluconate transport protein

Products: Proton [Cytoplasm]; gluconate [Cytoplasm] [C]

Alternate protein names: Gluconate permease; Gnt-I system [H]

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MPLFIVAIGVALLLLLMIRFKLNGFIALILVALAVGILQGMPVNKVIESIKNGVGGTLGSLALIMGFGAMLGKLLADCGG
AQRIATTLIDKFGQLYIQWAVVLTGFTVGFALFYEIGFVLMLPLVFSIAANARISLLYVGVPMAAALSVTHGFLPPHPGP
TAIAAIFHADMGRTLLYGTILAIPTVILAGPVYSRFLFHIDKPVPEGLYNPKKFTEQEMPSFAVSVWTSLVPVVLMALRA
VTEMVLPAGNPILAYTEFFGEPIMATFIAVLIAIFTFGLNRGRSMDEVMNTITDSIKIIAMMLLIIGGGGAFKQVLVDSG
VDKYIASLMEGSTISPLLLAWTIAATLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSHVNDPGFWL
FKEYFNLTIGETIRSWSVLETIIAVGGLVGCLLLNMVI

Sequences:

>Translated_438_residues
MPLFIVAIGVALLLLLMIRFKLNGFIALILVALAVGILQGMPVNKVIESIKNGVGGTLGSLALIMGFGAMLGKLLADCGG
AQRIATTLIDKFGQLYIQWAVVLTGFTVGFALFYEIGFVLMLPLVFSIAANARISLLYVGVPMAAALSVTHGFLPPHPGP
TAIAAIFHADMGRTLLYGTILAIPTVILAGPVYSRFLFHIDKPVPEGLYNPKKFTEQEMPSFAVSVWTSLVPVVLMALRA
VTEMVLPAGNPILAYTEFFGEPIMATFIAVLIAIFTFGLNRGRSMDEVMNTITDSIKIIAMMLLIIGGGGAFKQVLVDSG
VDKYIASLMEGSTISPLLLAWTIAATLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSHVNDPGFWL
FKEYFNLTIGETIRSWSVLETIIAVGGLVGCLLLNMVI
>Mature_437_residues
PLFIVAIGVALLLLLMIRFKLNGFIALILVALAVGILQGMPVNKVIESIKNGVGGTLGSLALIMGFGAMLGKLLADCGGA
QRIATTLIDKFGQLYIQWAVVLTGFTVGFALFYEIGFVLMLPLVFSIAANARISLLYVGVPMAAALSVTHGFLPPHPGPT
AIAAIFHADMGRTLLYGTILAIPTVILAGPVYSRFLFHIDKPVPEGLYNPKKFTEQEMPSFAVSVWTSLVPVVLMALRAV
TEMVLPAGNPILAYTEFFGEPIMATFIAVLIAIFTFGLNRGRSMDEVMNTITDSIKIIAMMLLIIGGGGAFKQVLVDSGV
DKYIASLMEGSTISPLLLAWTIAATLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSHVNDPGFWLF
KEYFNLTIGETIRSWSVLETIIAVGGLVGCLLLNMVI

Specific function: Part of the gluconate utilization system Gnt-I; high- affinity intake of gluconate [H]

COG id: COG2610

COG function: function code GE; H+/gluconate symporter and related permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gntP permease family [H]

Homologues:

Organism=Escherichia coli, GI48994935, Length=438, Percent_Identity=84.2465753424658, Blast_Score=713, Evalue=0.0,
Organism=Escherichia coli, GI1790716, Length=438, Percent_Identity=62.1004566210046, Blast_Score=549, Evalue=1e-157,
Organism=Escherichia coli, GI2367370, Length=449, Percent_Identity=48.9977728285078, Blast_Score=372, Evalue=1e-104,
Organism=Escherichia coli, GI1790776, Length=438, Percent_Identity=40.1826484018265, Blast_Score=339, Evalue=2e-94,
Organism=Escherichia coli, GI1788707, Length=434, Percent_Identity=35.9447004608295, Blast_Score=268, Evalue=6e-73,
Organism=Escherichia coli, GI48994939, Length=434, Percent_Identity=35.4838709677419, Blast_Score=258, Evalue=4e-70,
Organism=Escherichia coli, GI1789097, Length=440, Percent_Identity=32.7272727272727, Blast_Score=219, Evalue=3e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003474 [H]

Pfam domain/function: PF02447 GntP_permease [H]

EC number: NA

Molecular weight: Translated: 46306; Mature: 46175

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLFIVAIGVALLLLLMIRFKLNGFIALILVALAVGILQGMPVNKVIESIKNGVGGTLGS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHH
LALIMGFGAMLGKLLADCGGAQRIATTLIDKFGQLYIQWAVVLTGFTVGFALFYEIGFVL
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLPLVFSIAANARISLLYVGVPMAAALSVTHGFLPPHPGPTAIAAIFHADMGRTLLYGTI
HHHHHHHHHCCCEEEEEEECCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHH
LAIPTVILAGPVYSRFLFHIDKPVPEGLYNPKKFTEQEMPSFAVSVWTSLVPVVLMALRA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTEMVLPAGNPILAYTEFFGEPIMATFIAVLIAIFTFGLNRGRSMDEVMNTITDSIKIIA
HHHHHHCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
MMLLIIGGGGAFKQVLVDSGVDKYIASLMEGSTISPLLLAWTIAATLRIALGSATVAAIT
HHHHHHCCCHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
AGGIAAPLIATTGVSPELMVIAVGSGSVIFSHVNDPGFWLFKEYFNLTIGETIRSWSVLE
HCCHHHHHHHHCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHH
TIIAVGGLVGCLLLNMVI
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PLFIVAIGVALLLLLMIRFKLNGFIALILVALAVGILQGMPVNKVIESIKNGVGGTLGS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHH
LALIMGFGAMLGKLLADCGGAQRIATTLIDKFGQLYIQWAVVLTGFTVGFALFYEIGFVL
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLPLVFSIAANARISLLYVGVPMAAALSVTHGFLPPHPGPTAIAAIFHADMGRTLLYGTI
HHHHHHHHHCCCEEEEEEECCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHH
LAIPTVILAGPVYSRFLFHIDKPVPEGLYNPKKFTEQEMPSFAVSVWTSLVPVVLMALRA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTEMVLPAGNPILAYTEFFGEPIMATFIAVLIAIFTFGLNRGRSMDEVMNTITDSIKIIA
HHHHHHCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
MMLLIIGGGGAFKQVLVDSGVDKYIASLMEGSTISPLLLAWTIAATLRIALGSATVAAIT
HHHHHHCCCHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
AGGIAAPLIATTGVSPELMVIAVGSGSVIFSHVNDPGFWLFKEYFNLTIGETIRSWSVLE
HCCHHHHHHHHCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHH
TIIAVGGLVGCLLLNMVI
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; gluconate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + gluconate [Periplasm] = Proton [Cytoplasm] + gluconate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 2845225; 7984428; 3040894; 9045817 [H]