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The map label for this gene is envZ

Identifier: 85060296

GI number: 85060296

Start: 3981398

End: 3982780

Strand: Reverse

Name: envZ

Synonym: SG2318

Alternate gene names: 85060296

Gene position: 3982780-3981398 (Counterclockwise)

Preceding gene: 85060297

Following gene: 85060290

Centisome position: 95.48

GC content: 57.27

Gene sequence:

>1383_bases
ATGATGCGATGGCGCATCTCTCCGCGCAGTTCCTTCGCCCGCAGCCTCTTGTTAATCGTTACCTTGCTGTTCGTCAGCCT
GGTAACGACCTACCTGGTGGTCTTGAATTTCGCCATCTTACCGAGTCTGCAACAGTTCAATAAAGTGCTGGCTTATGAAG
TCAGAATGTTGATGACAGACCAGTTGCAGCTGGAGGACGGTACCTTACTGGAAGTGCCGCCGGCGTTCCGGCGCGAAATT
TACCGTGAATTGGGTATCTCGCTGTATACCAATCCAGCCGCGGAAGAGAGCGGCTTGCGCTGGGCGCAACATTATGAATT
CCTGAGCCAGCAAATGGCTCAGCAGCTCGGCAGCCCGACCGATGTACGCGTCGAAGTCACCAAAAATTCCCCAGTTGTCT
GGCTGAAAACCTGGTTATCGCCGGATATCTGGGTACGCATTCCGTTGACCGAAATCCATCAGGGCGGCTTCTCGCCGTTA
TTCCGTTACACCCTGGCGATTATGTTGCTGGCTATCGGCGGTGCCTGGCTGTTTATCCGTATTCAAAACCGGCCGCTGGT
GGATTTGGAACATGCCGCGCTGCAGGTGGGTAAGGGCATCATTCCACCGCCGCTGCGGGAGTATGGCGCCTCGGAGGTGC
GCTCGGTGACGCGGGCGTTCAACCAGATGACGGCCGGCGTCAAGCTATTGGCGGATGACCGGACCTTGTTGATGGCCGGC
GTCAGCCATGATTTGCGCACGCCGCTGACCCGTATCCGCCTGGCGACGGAAATGATGAGTGCGGAAGACGGCTATCTTGC
CGAGTCGATCAATAAAGATATCGAAGAGTGCAACGCTATCATCGAGCAGTTCATCGATTATCTGCGCACCGGCCAGGAGA
TGCAGACCGAAATGGCGGATCTCAACAGCATCCTGACCGAGGTGGTGGCGGCGGAGAGCGGCTATGAGCGCGTGATCGAA
ACCGATATTTGCGGCGAGGAGCTTATTGTTAACGTCAATCCGCTGTCCATTAAACGCGCGGCGCTGAATATGGTGGTGAA
TGCCGCGCGCTACGGCAACGGCTGTATAAAGGTAAATAGCGGACGCGAAACCAATCGGGTCTGGTTTGAGGTCGAGGATG
ATGGTCCGGGCATCCAGCCGGATCAATTACAATATTTATTTCAACCGTTCGTGCGCGGCGACAGCGCGCGCAGCACCAGC
GGTACCGGCCTCGGTCTGGCCATTGTCCAGCGCATCATCGATGCGCATGCCGGTAAGCTGGAAATCGGCCGCAGCGCGCG
GCAGGGCTTGCGTATCCGCGCCTATTTGCCGCTACCGGTCGCGTCGGTGGATCACGGTGCCAGCGTCAGTAAAGCCAAAG
CCGCCGGCAAAAGCGCCGACTAA

Upstream 100 bases:

>100_bases
CGCGTCTGCGGCGCATGGTGGAAGAAGATCCCGCCCATCCGCGCTATATTCAGACCGTATGGGGTCTGGGCTATGTCTTT
GTGCCGGATGGCAGTAAAGC

Downstream 100 bases:

>100_bases
GCGGCGGCTTAGGCAAAGTGCGCCGCCGCCGGGCCGGTCAACGTCATACGCGTGACGGTATGGCCTCTTCCTGAGCGGCT
GTCTTACCAAGGTGTCAGGG

Product: osmolarity sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MMRWRISPRSSFARSLLLIVTLLFVSLVTTYLVVLNFAILPSLQQFNKVLAYEVRMLMTDQLQLEDGTLLEVPPAFRREI
YRELGISLYTNPAAEESGLRWAQHYEFLSQQMAQQLGSPTDVRVEVTKNSPVVWLKTWLSPDIWVRIPLTEIHQGGFSPL
FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTRAFNQMTAGVKLLADDRTLLMAG
VSHDLRTPLTRIRLATEMMSAEDGYLAESINKDIEECNAIIEQFIDYLRTGQEMQTEMADLNSILTEVVAAESGYERVIE
TDICGEELIVNVNPLSIKRAALNMVVNAARYGNGCIKVNSGRETNRVWFEVEDDGPGIQPDQLQYLFQPFVRGDSARSTS
GTGLGLAIVQRIIDAHAGKLEIGRSARQGLRIRAYLPLPVASVDHGASVSKAKAAGKSAD

Sequences:

>Translated_460_residues
MMRWRISPRSSFARSLLLIVTLLFVSLVTTYLVVLNFAILPSLQQFNKVLAYEVRMLMTDQLQLEDGTLLEVPPAFRREI
YRELGISLYTNPAAEESGLRWAQHYEFLSQQMAQQLGSPTDVRVEVTKNSPVVWLKTWLSPDIWVRIPLTEIHQGGFSPL
FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTRAFNQMTAGVKLLADDRTLLMAG
VSHDLRTPLTRIRLATEMMSAEDGYLAESINKDIEECNAIIEQFIDYLRTGQEMQTEMADLNSILTEVVAAESGYERVIE
TDICGEELIVNVNPLSIKRAALNMVVNAARYGNGCIKVNSGRETNRVWFEVEDDGPGIQPDQLQYLFQPFVRGDSARSTS
GTGLGLAIVQRIIDAHAGKLEIGRSARQGLRIRAYLPLPVASVDHGASVSKAKAAGKSAD
>Mature_460_residues
MMRWRISPRSSFARSLLLIVTLLFVSLVTTYLVVLNFAILPSLQQFNKVLAYEVRMLMTDQLQLEDGTLLEVPPAFRREI
YRELGISLYTNPAAEESGLRWAQHYEFLSQQMAQQLGSPTDVRVEVTKNSPVVWLKTWLSPDIWVRIPLTEIHQGGFSPL
FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTRAFNQMTAGVKLLADDRTLLMAG
VSHDLRTPLTRIRLATEMMSAEDGYLAESINKDIEECNAIIEQFIDYLRTGQEMQTEMADLNSILTEVVAAESGYERVIE
TDICGEELIVNVNPLSIKRAALNMVVNAARYGNGCIKVNSGRETNRVWFEVEDDGPGIQPDQLQYLFQPFVRGDSARSTS
GTGLGLAIVQRIIDAHAGKLEIGRSARQGLRIRAYLPLPVASVDHGASVSKAKAAGKSAD

Specific function: Member of the two-component regulatory system envZ/ompR involved in the regulation of osmoregulation (genes ompF and ompC). EnvZ functions as a membrane-associated protein kinase that phosphorylates ompR in response to environmental signals [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1789808, Length=440, Percent_Identity=84.5454545454545, Blast_Score=759, Evalue=0.0,
Organism=Escherichia coli, GI1790346, Length=274, Percent_Identity=31.021897810219, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1787894, Length=272, Percent_Identity=30.8823529411765, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI87082128, Length=291, Percent_Identity=25.085910652921, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1786912, Length=217, Percent_Identity=27.6497695852535, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1786600, Length=245, Percent_Identity=30.2040816326531, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1790436, Length=220, Percent_Identity=24.5454545454545, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1788549, Length=215, Percent_Identity=28.8372093023256, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786783, Length=292, Percent_Identity=27.7397260273973, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1788393, Length=246, Percent_Identity=27.2357723577236, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1790551, Length=255, Percent_Identity=26.6666666666667, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI145693157, Length=239, Percent_Identity=24.6861924686192, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1789149, Length=225, Percent_Identity=28.8888888888889, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87081816, Length=272, Percent_Identity=26.8382352941176, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1789403, Length=246, Percent_Identity=25.2032520325203, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1787374, Length=230, Percent_Identity=24.3478260869565, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 51215; Mature: 51215

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRWRISPRSSFARSLLLIVTLLFVSLVTTYLVVLNFAILPSLQQFNKVLAYEVRMLMTD
CCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
QLQLEDGTLLEVPPAFRREIYRELGISLYTNPAAEESGLRWAQHYEFLSQQMAQQLGSPT
CEEECCCCEEECCHHHHHHHHHHCCCEEECCCCCCHHCCHHHHHHHHHHHHHHHHCCCCC
DVRVEVTKNSPVVWLKTWLSPDIWVRIPLTEIHQGGFSPLFRYTLAIMLLAIGGAWLFIR
CEEEEEECCCCEEEEEECCCCCEEEEEEHHHHCCCCCHHHHHHHHHHHHHHHCCEEEEEE
IQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTRAFNQMTAGVKLLADDRTLLMAG
ECCCCCCHHHHHHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHHHCCEEEEECCCEEEEEC
VSHDLRTPLTRIRLATEMMSAEDGYLAESINKDIEECNAIIEQFIDYLRTGQEMQTEMAD
CCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LNSILTEVVAAESGYERVIETDICGEELIVNVNPLSIKRAALNMVVNAARYGNGCIKVNS
HHHHHHHHHHHCCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCEEEECC
GRETNRVWFEVEDDGPGIQPDQLQYLFQPFVRGDSARSTSGTGLGLAIVQRIIDAHAGKL
CCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCE
EIGRSARQGLRIRAYLPLPVASVDHGASVSKAKAAGKSAD
EECHHHHCCCEEEEEECCCCCCCCCCCCHHHHHCCCCCCC
>Mature Secondary Structure
MMRWRISPRSSFARSLLLIVTLLFVSLVTTYLVVLNFAILPSLQQFNKVLAYEVRMLMTD
CCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
QLQLEDGTLLEVPPAFRREIYRELGISLYTNPAAEESGLRWAQHYEFLSQQMAQQLGSPT
CEEECCCCEEECCHHHHHHHHHHCCCEEECCCCCCHHCCHHHHHHHHHHHHHHHHCCCCC
DVRVEVTKNSPVVWLKTWLSPDIWVRIPLTEIHQGGFSPLFRYTLAIMLLAIGGAWLFIR
CEEEEEECCCCEEEEEECCCCCEEEEEEHHHHCCCCCHHHHHHHHHHHHHHHCCEEEEEE
IQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTRAFNQMTAGVKLLADDRTLLMAG
ECCCCCCHHHHHHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHHHCCEEEEECCCEEEEEC
VSHDLRTPLTRIRLATEMMSAEDGYLAESINKDIEECNAIIEQFIDYLRTGQEMQTEMAD
CCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LNSILTEVVAAESGYERVIETDICGEELIVNVNPLSIKRAALNMVVNAARYGNGCIKVNS
HHHHHHHHHHHCCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCEEEECC
GRETNRVWFEVEDDGPGIQPDQLQYLFQPFVRGDSARSTSGTGLGLAIVQRIIDAHAGKL
CCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCE
EIGRSARQGLRIRAYLPLPVASVDHGASVSKAKAAGKSAD
EECHHHHCCCEEEEEECCCCCCCCCCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 6292200; 2997120; 9278503; 8132603; 2277041; 2824492; 1323560; 9817206; 10426948 [H]