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The map label for this gene is nudE

Identifier: 85060290

GI number: 85060290

Start: 3974272

End: 3974835

Strand: Reverse

Name: nudE

Synonym: SG2312

Alternate gene names: 85060290

Gene position: 3974835-3974272 (Counterclockwise)

Preceding gene: 85060296

Following gene: 85060287

Centisome position: 95.29

GC content: 57.45

Gene sequence:

>564_bases
ATGGATAAGAATCTGCAAAAACCCAAAATACATCGCGTGGCGACCGTTGCCCAGTCCCGCCTGTTCACGGTTGAGTCCGT
CGATCTGGAGTTCAGCAACGGCGCGCGGCGGGTCTATGAGCGCATGAAGCCTTCCCATCACGAGACGGTGATGGTCGTAC
CGGTGCTGGACGACCATCTACTGCTGATCCGTGAATACGGCGCCGGCATCGAGGATTATGAATTGGGGTTTCCAAAAGGA
CTTATCGACCCCGGCGAAGTGGTGATGGAAGCGGCCAACCGCGAGCTGATGGAGGAGGCGGGCTTTGGAGGGCGCAACTT
TACGTTTTTGCGCCGCTTGAGTATGGCGCCGTCCTATTTCTCCAGCAAAATGCACATTGTTGTGGCGCAAAATTTGTATC
CCCAGCGGTTGCAGGGCGATGAGCCGGAACCGTTGCCGCTGGTGCGCTGGCCGATAAGCCGTATGCTGGCGCTGCTTGAC
GAGCCCGATTTTTGCGAGGCGCGTAACGTGAGCGCGCTGTTTCTCGCCCATGCCTGGCTTAATCGTACACCGGGAGAAGA
GTAA

Upstream 100 bases:

>100_bases
GACAGGTTGCGGCCCGGTCCATTCTTAACGCACAATGTTGACCAGGCAGGCCGATGGGCGGGCATCTCCTGGCCATCCAG
TGCGCAATCTCGGGAACGAT

Downstream 100 bases:

>100_bases
GCCCGGGCCGGGTCCAAAGACATCCGGCCCGGCGAGCGGTCAGAACAGTTCCTGCTGCTGGCCGTTGTCCATCAGCGTCG
TGCCGACTTCATGTACTACC

Product: ADP-ribose diphosphatase NudE

Products: AMP; D-Ribose 5- Phosphate. [C]

Alternate protein names: NA

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MDKNLQKPKIHRVATVAQSRLFTVESVDLEFSNGARRVYERMKPSHHETVMVVPVLDDHLLLIREYGAGIEDYELGFPKG
LIDPGEVVMEAANRELMEEAGFGGRNFTFLRRLSMAPSYFSSKMHIVVAQNLYPQRLQGDEPEPLPLVRWPISRMLALLD
EPDFCEARNVSALFLAHAWLNRTPGEE

Sequences:

>Translated_187_residues
MDKNLQKPKIHRVATVAQSRLFTVESVDLEFSNGARRVYERMKPSHHETVMVVPVLDDHLLLIREYGAGIEDYELGFPKG
LIDPGEVVMEAANRELMEEAGFGGRNFTFLRRLSMAPSYFSSKMHIVVAQNLYPQRLQGDEPEPLPLVRWPISRMLALLD
EPDFCEARNVSALFLAHAWLNRTPGEE
>Mature_187_residues
MDKNLQKPKIHRVATVAQSRLFTVESVDLEFSNGARRVYERMKPSHHETVMVVPVLDDHLLLIREYGAGIEDYELGFPKG
LIDPGEVVMEAANRELMEEAGFGGRNFTFLRRLSMAPSYFSSKMHIVVAQNLYPQRLQGDEPEPLPLVRWPISRMLALLD
EPDFCEARNVSALFLAHAWLNRTPGEE

Specific function: Active on adenosine(5')triphospho(5')adenosine (Ap3A), ADP-ribose, NADH, adenosine(5')diphospho(5')adenosine (Ap2A) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1789800, Length=180, Percent_Identity=72.7777777777778, Blast_Score=278, Evalue=1e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 3.6.1.- [C]

Molecular weight: Translated: 21335; Mature: 21335

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKNLQKPKIHRVATVAQSRLFTVESVDLEFSNGARRVYERMKPSHHETVMVVPVLDDHL
CCCCCCCCHHHHHHHHHHHHEEEEEEECEEECCCHHHHHHHHCCCCCCEEEEEEECCCCE
LLIREYGAGIEDYELGFPKGLIDPGEVVMEAANRELMEEAGFGGRNFTFLRRLSMAPSYF
EEEHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHH
SSKMHIVVAQNLYPQRLQGDEPEPLPLVRWPISRMLALLDEPDFCEARNVSALFLAHAWL
CCCEEEEEECCCCHHHHCCCCCCCCCEEHHCHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
NRTPGEE
CCCCCCC
>Mature Secondary Structure
MDKNLQKPKIHRVATVAQSRLFTVESVDLEFSNGARRVYERMKPSHHETVMVVPVLDDHL
CCCCCCCCHHHHHHHHHHHHEEEEEEECEEECCCHHHHHHHHCCCCCCEEEEEEECCCCE
LLIREYGAGIEDYELGFPKGLIDPGEVVMEAANRELMEEAGFGGRNFTFLRRLSMAPSYF
EEEHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHH
SSKMHIVVAQNLYPQRLQGDEPEPLPLVRWPISRMLALLDEPDFCEARNVSALFLAHAWL
CCCEEEEEECCCCHHHHCCCCCCCCCEEHHCHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
NRTPGEE
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ADP-Ribose; H2O [C]

Specific reaction: ADP-Ribose + H2O = AMP + D-Ribose 5- Phosphate. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 9452430 [H]