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The map label for this gene is pyrE

Identifier: 85060190

GI number: 85060190

Start: 3843338

End: 3843979

Strand: Reverse

Name: pyrE

Synonym: SG2212

Alternate gene names: 85060190

Gene position: 3843979-3843338 (Counterclockwise)

Preceding gene: 85060191

Following gene: 85060186

Centisome position: 92.16

GC content: 53.74

Gene sequence:

>642_bases
GTGAAAGAGTACCAGAGTAAATTTATCGAGTATGCATTAAACAAACAGGTATTAAAATTCGGCGAATTTACGCTTAAATC
CGGCCGTATCAGCCCCTATTTTTTTAATGCCGGTTTGTTTAACACCGGTCGCGATCTGGCTTTCCTGGGGCGGGTTTACG
CCGCGGCGCTGATGGATTCCGGCGTGCGTTTCGATGTGTTATTCGGGCCGGCCTACAAGGGGATTCCCATTGCGACGACC
ACCGCGGTGGCACTGGCTGAGCAACATCAACGGGACGTTCCCTACTGTTTTAATCGCAAGGAAGCGAAGGACCACGGCGA
AGGCGGTACGCTGGTCGGCAGCCCGCTTCAGGGTAAAGTGATGCTGGTGGATGATGTTATCACCGCGGGTACCGCTATTC
GCGAGTCGATGGAGATTATCGCCGCGCACCAGGCAACGCTTGCCGGTGTAATGATTTCCCTGGACAGGCAGGAAAAAGGG
CGCAGTGAGATTTCCGCTATTCAGGAGGTGGAGCGGGATTATCACTGCCAGGTGCTTGCCATCATCACGTTGGCGGATTT
AATCGCCTATCTGGAAGAAAAAGGCGAAATGGCCGGCCATCTCGCCGCGGTCCGTGCCTATCAGCAGCAATACGGCATTT
AA

Upstream 100 bases:

>100_bases
GCATCTTGGTAACGGCCATTTTATCATCCCCCCGGCGCTGATACCTTTCAGGTACTCTGCACCATAGCTTGTCCAACCCA
CCGGTGAGGAGTAACAGTCC

Downstream 100 bases:

>100_bases
ACGGGCCGGGCGAGACGGGCGTTCCGCGTTGGTGCCGATCGTTGTAAAGGTGCGTCGCGGCGCTCAGTGCAGCTGCGCTA
CCAAAAGCGGCCAGCGTAGC

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MKEYQSKFIEYALNKQVLKFGEFTLKSGRISPYFFNAGLFNTGRDLAFLGRVYAAALMDSGVRFDVLFGPAYKGIPIATT
TAVALAEQHQRDVPYCFNRKEAKDHGEGGTLVGSPLQGKVMLVDDVITAGTAIRESMEIIAAHQATLAGVMISLDRQEKG
RSEISAIQEVERDYHCQVLAIITLADLIAYLEEKGEMAGHLAAVRAYQQQYGI

Sequences:

>Translated_213_residues
MKEYQSKFIEYALNKQVLKFGEFTLKSGRISPYFFNAGLFNTGRDLAFLGRVYAAALMDSGVRFDVLFGPAYKGIPIATT
TAVALAEQHQRDVPYCFNRKEAKDHGEGGTLVGSPLQGKVMLVDDVITAGTAIRESMEIIAAHQATLAGVMISLDRQEKG
RSEISAIQEVERDYHCQVLAIITLADLIAYLEEKGEMAGHLAAVRAYQQQYGI
>Mature_213_residues
MKEYQSKFIEYALNKQVLKFGEFTLKSGRISPYFFNAGLFNTGRDLAFLGRVYAAALMDSGVRFDVLFGPAYKGIPIATT
TAVALAEQHQRDVPYCFNRKEAKDHGEGGTLVGSPLQGKVMLVDDVITAGTAIRESMEIIAAHQATLAGVMISLDRQEKG
RSEISAIQEVERDYHCQVLAIITLADLIAYLEEKGEMAGHLAAVRAYQQQYGI

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) [H]

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507835, Length=181, Percent_Identity=25.414364640884, Blast_Score=81, Evalue=8e-16,
Organism=Escherichia coli, GI1790073, Length=213, Percent_Identity=81.6901408450704, Blast_Score=363, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17508631, Length=196, Percent_Identity=27.5510204081633, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323530, Length=220, Percent_Identity=48.6363636363636, Blast_Score=205, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6323927, Length=221, Percent_Identity=44.7963800904977, Blast_Score=192, Evalue=4e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.4.2.10 [H]

Molecular weight: Translated: 23458; Mature: 23458

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00136 SUBTILASE_ASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEYQSKFIEYALNKQVLKFGEFTLKSGRISPYFFNAGLFNTGRDLAFLGRVYAAALMDS
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCC
GVRFDVLFGPAYKGIPIATTTAVALAEQHQRDVPYCFNRKEAKDHGEGGTLVGSPLQGKV
CCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCEECCCCCCCE
MLVDDVITAGTAIRESMEIIAAHQATLAGVMISLDRQEKGRSEISAIQEVERDYHCQVLA
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHCHHHHH
IITLADLIAYLEEKGEMAGHLAAVRAYQQQYGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKEYQSKFIEYALNKQVLKFGEFTLKSGRISPYFFNAGLFNTGRDLAFLGRVYAAALMDS
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCC
GVRFDVLFGPAYKGIPIATTTAVALAEQHQRDVPYCFNRKEAKDHGEGGTLVGSPLQGKV
CCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCEECCCCCCCE
MLVDDVITAGTAIRESMEIIAAHQATLAGVMISLDRQEKGRSEISAIQEVERDYHCQVLA
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHCHHHHH
IITLADLIAYLEEKGEMAGHLAAVRAYQQQYGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA