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The map label for this gene is rph

Identifier: 85060191

GI number: 85060191

Start: 3845286

End: 3846005

Strand: Reverse

Name: rph

Synonym: SG2213

Alternate gene names: 85060191

Gene position: 3846005-3845286 (Counterclockwise)

Preceding gene: 85060193

Following gene: 85060190

Centisome position: 92.2

GC content: 58.61

Gene sequence:

>720_bases
ATGCGTCCAGCAGGCCGAAGCGTACAGCAAGTACGCCCAGTAACGATTACCCGCCATTTTACCCGTCATGCGGAAGGCTC
AGTGCTGATAGCATTCGGCGACACCCGCGTGCTGTGTACTGCGTCGGTGGAAGAGGGGGTGCCGCGGTTTCTCAAGGGGC
AGGGGCAGGGCTGGATCACCGCCGAATACGGCATGCTGCCGCGCGCAACTCATACCCGCAATCCTCGCGAAGCGGCGAAG
GGCAAGCAAGGCGGCCGTACCCTGGAAATTCAGCGACTTATTGCCCGTTCGCTGCGCGCCGCGGTGGATTTAACCAAATT
AGGTGAATATACCATTACCCTGGATTGCGACGTGATACAGGCCGACGGCGGCACCCGAACGGCGTCCATTACCGGTGCCT
GCGTGGCGCTGGCCGACGCCCTGAGCAGCATGCAAAAGAAAGGTCAGCTAAAAACCAACCCGATGAAAGGGTTCGTGGCA
GCGATTTCGGTGGGTATTGTGGCCGGTGAAGCGGTGTGTGATTTGGAGTATGTCGAAGATTCGGCTGCTGAAACCGATAT
GAACGTGGTGATGATGGAAGACGGTCGCATGATTGAGGTACAAGGTACCGCGGAAGGAGAACCTTTCAGTCAGCAGGAAC
TCAACACGCTGCTTGAGCTGGCCCGTGGCGGCATCGATAGCCTGGTTCAGGCGCAAAAAGCCGCGCTGGTGGGAGCATAG

Upstream 100 bases:

>100_bases
CTGGGATTATAGCCCCGCTGGGGCAGGCAAGATAGGCATTAGGCGGTGAAGTCCGTATAATGCGCAGCCAACCTTTGATT
TGCGAGCCGGAGAAACCCCT

Downstream 100 bases:

>100_bases
TCCACCGCGATCGCGGGCTGTTAGCGCCTGCTTCGTGATGTTGATCACCGTTTGGCGCCCGCTACGCTCGAGTGTTGATT
AAGCAGGCGTCTGGGCGGGC

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MRPAGRSVQQVRPVTITRHFTRHAEGSVLIAFGDTRVLCTASVEEGVPRFLKGQGQGWITAEYGMLPRATHTRNPREAAK
GKQGGRTLEIQRLIARSLRAAVDLTKLGEYTITLDCDVIQADGGTRTASITGACVALADALSSMQKKGQLKTNPMKGFVA
AISVGIVAGEAVCDLEYVEDSAAETDMNVVMMEDGRMIEVQGTAEGEPFSQQELNTLLELARGGIDSLVQAQKAALVGA

Sequences:

>Translated_239_residues
MRPAGRSVQQVRPVTITRHFTRHAEGSVLIAFGDTRVLCTASVEEGVPRFLKGQGQGWITAEYGMLPRATHTRNPREAAK
GKQGGRTLEIQRLIARSLRAAVDLTKLGEYTITLDCDVIQADGGTRTASITGACVALADALSSMQKKGQLKTNPMKGFVA
AISVGIVAGEAVCDLEYVEDSAAETDMNVVMMEDGRMIEVQGTAEGEPFSQQELNTLLELARGGIDSLVQAQKAALVGA
>Mature_239_residues
MRPAGRSVQQVRPVTITRHFTRHAEGSVLIAFGDTRVLCTASVEEGVPRFLKGQGQGWITAEYGMLPRATHTRNPREAAK
GKQGGRTLEIQRLIARSLRAAVDLTKLGEYTITLDCDVIQADGGTRTASITGACVALADALSSMQKKGQLKTNPMKGFVA
AISVGIVAGEAVCDLEYVEDSAAETDMNVVMMEDGRMIEVQGTAEGEPFSQQELNTLLELARGGIDSLVQAQKAALVGA

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family

Homologues:

Organism=Escherichia coli, GI157672248, Length=228, Percent_Identity=82.0175438596491, Blast_Score=379, Evalue=1e-107,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNPH_SODGM (Q2NQT7)

Other databases:

- EMBL:   AP008232
- RefSeq:   YP_455893.1
- ProteinModelPortal:   Q2NQT7
- SMR:   Q2NQT7
- STRING:   Q2NQT7
- GeneID:   3868758
- GenomeReviews:   AP008232_GR
- KEGG:   sgl:SG2213
- NMPDR:   fig|343509.6.peg.4860
- eggNOG:   COG0689
- HOGENOM:   HBG737187
- OMA:   MLPRATG
- PhylomeDB:   Q2NQT7
- ProtClustDB:   PRK00173
- BioCyc:   SGLO343509:SG2213-MONOMER
- HAMAP:   MF_00564
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336
- TIGRFAMs:   TIGR01966

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.56

Molecular weight: Translated: 25473; Mature: 25473

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPAGRSVQQVRPVTITRHFTRHAEGSVLIAFGDTRVLCTASVEEGVPRFLKGQGQGWIT
CCCCCCCHHHCCCEEEEHHHHHCCCCCEEEEECCCEEEEEECHHHCCHHHHCCCCCEEEE
AEYGMLPRATHTRNPREAAKGKQGGRTLEIQRLIARSLRAAVDLTKLGEYTITLDCDVIQ
ECCCCCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEE
ADGGTRTASITGACVALADALSSMQKKGQLKTNPMKGFVAAISVGIVAGEAVCDLEYVED
CCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHC
SAAETDMNVVMMEDGRMIEVQGTAEGEPFSQQELNTLLELARGGIDSLVQAQKAALVGA
CCCCCCCEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRPAGRSVQQVRPVTITRHFTRHAEGSVLIAFGDTRVLCTASVEEGVPRFLKGQGQGWIT
CCCCCCCHHHCCCEEEEHHHHHCCCCCEEEEECCCEEEEEECHHHCCHHHHCCCCCEEEE
AEYGMLPRATHTRNPREAAKGKQGGRTLEIQRLIARSLRAAVDLTKLGEYTITLDCDVIQ
ECCCCCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEE
ADGGTRTASITGACVALADALSSMQKKGQLKTNPMKGFVAAISVGIVAGEAVCDLEYVED
CCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHC
SAAETDMNVVMMEDGRMIEVQGTAEGEPFSQQELNTLLELARGGIDSLVQAQKAALVGA
CCCCCCCEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA