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The map label for this gene is gcvH

Identifier: 85059979

GI number: 85059979

Start: 3457728

End: 3458123

Strand: Reverse

Name: gcvH

Synonym: SG2001

Alternate gene names: 85059979

Gene position: 3458123-3457728 (Counterclockwise)

Preceding gene: 85059980

Following gene: 85059978

Centisome position: 82.91

GC content: 53.79

Gene sequence:

>396_bases
ATGAGCAATGTGCCGACAGAATTAAAATATACGGCATCCCATGAGTGGGTGCTTAATGAAGGCGATGGTGTTTACTGTGT
CGGGATCACTGAGCATGCCCAGGAACTGTTGGGCGATATGGTGTTTATGGACCTGCCCGAGGTTGGCGCCACGTTTTCCG
CCGGCGAAGATTGCGCGGTGGCGGAATCGGTGAAAGCCGCATCGGATATTTATGCGCCTATCAGCGGCGAAATCGTGGCG
GTGAACGATGATTTGGAAGCGTCGCCGGAGTTGGTCAACAGCGCGCCTTACACCGATGGCTGGCTGTTTAAAATCAAGGC
GACCGACGACAGCGAAGTGAACGAATTGCTCGATGCCGCCGCTTATCAGGCCGCTATTGACGAAGAAGACGAATAA

Upstream 100 bases:

>100_bases
TCCGTAACCGCGAAATGCCGGTTAAGGTCACCAAACCCGGTTTTGTGCGCGCCGGTAAAGCGGTTGCGCAATAACTAACT
TTGTCAGAGTGAGGATGGCG

Downstream 100 bases:

>100_bases
CGCCGACGCCCCGGCCGCGACCGCCGGGGCGCACCTTGCGCTTACCCTTTTCCGGTCCCATGCCCGATGTAGGAATTGCT
GTCCATGACCCAGACTCTTA

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 131; Mature: 130

Protein sequence:

>131_residues
MSNVPTELKYTASHEWVLNEGDGVYCVGITEHAQELLGDMVFMDLPEVGATFSAGEDCAVAESVKAASDIYAPISGEIVA
VNDDLEASPELVNSAPYTDGWLFKIKATDDSEVNELLDAAAYQAAIDEEDE

Sequences:

>Translated_131_residues
MSNVPTELKYTASHEWVLNEGDGVYCVGITEHAQELLGDMVFMDLPEVGATFSAGEDCAVAESVKAASDIYAPISGEIVA
VNDDLEASPELVNSAPYTDGWLFKIKATDDSEVNELLDAAAYQAAIDEEDE
>Mature_130_residues
SNVPTELKYTASHEWVLNEGDGVYCVGITEHAQELLGDMVFMDLPEVGATFSAGEDCAVAESVKAASDIYAPISGEIVAV
NDDLEASPELVNSAPYTDGWLFKIKATDDSEVNELLDAAAYQAAIDEEDE

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=123, Percent_Identity=44.7154471544715, Blast_Score=112, Evalue=8e-26,
Organism=Homo sapiens, GI89057342, Length=123, Percent_Identity=43.9024390243902, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI1789271, Length=129, Percent_Identity=75.1937984496124, Blast_Score=201, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI17551294, Length=122, Percent_Identity=45.9016393442623, Blast_Score=113, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17507493, Length=113, Percent_Identity=49.5575221238938, Blast_Score=112, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6319272, Length=132, Percent_Identity=38.6363636363636, Blast_Score=100, Evalue=1e-22,
Organism=Drosophila melanogaster, GI17865652, Length=123, Percent_Identity=42.2764227642276, Blast_Score=106, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_SODGM (Q2NRE9)

Other databases:

- EMBL:   AP008232
- RefSeq:   YP_455681.1
- ProteinModelPortal:   Q2NRE9
- SMR:   Q2NRE9
- STRING:   Q2NRE9
- GeneID:   3867082
- GenomeReviews:   AP008232_GR
- KEGG:   sgl:SG2001
- NMPDR:   fig|343509.6.peg.4329
- eggNOG:   COG0509
- HOGENOM:   HBG693789
- OMA:   SASEYEQ
- PhylomeDB:   Q2NRE9
- ProtClustDB:   PRK01202
- BioCyc:   SGLO343509:SG2001-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 14035; Mature: 13904

Theoretical pI: Translated: 3.47; Mature: 3.47

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNVPTELKYTASHEWVLNEGDGVYCVGITEHAQELLGDMVFMDLPEVGATFSAGEDCAV
CCCCCCCEEEECCCCEEEECCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
AESVKAASDIYAPISGEIVAVNDDLEASPELVNSAPYTDGWLFKIKATDDSEVNELLDAA
HHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHCCCCCCCCEEEEEEECCCHHHHHHHHHH
AYQAAIDEEDE
HHHHHCCCCCC
>Mature Secondary Structure 
SNVPTELKYTASHEWVLNEGDGVYCVGITEHAQELLGDMVFMDLPEVGATFSAGEDCAV
CCCCCCEEEECCCCEEEECCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
AESVKAASDIYAPISGEIVAVNDDLEASPELVNSAPYTDGWLFKIKATDDSEVNELLDAA
HHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHCCCCCCCCEEEEEEECCCHHHHHHHHHH
AYQAAIDEEDE
HHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA